[go: up one dir, main page]

MX2022005777A - Etiquetado utilizando transposomas inmovilizados con ligadores. - Google Patents

Etiquetado utilizando transposomas inmovilizados con ligadores.

Info

Publication number
MX2022005777A
MX2022005777A MX2022005777A MX2022005777A MX2022005777A MX 2022005777 A MX2022005777 A MX 2022005777A MX 2022005777 A MX2022005777 A MX 2022005777A MX 2022005777 A MX2022005777 A MX 2022005777A MX 2022005777 A MX2022005777 A MX 2022005777A
Authority
MX
Mexico
Prior art keywords
ligators
labeling
compositions
transposomes
immobilized
Prior art date
Application number
MX2022005777A
Other languages
English (en)
Inventor
Grace Desantis
Stephen M Gross
jian-sen Li
Natalie Morrell
Andrew Slatter
Kevin Shen
Samantha Snow
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2022005777A publication Critical patent/MX2022005777A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Peptides Or Proteins (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente descripción se refiere a métodos, composiciones, y equipos para tratar ácidos nucleicos diana, incluyendo métodos y composiciones para fragmentar y etiquetar ácido nucleico (por ejemplo, ADN) utilizando complejos de transposomas enlazados a un soporte sólido.
MX2022005777A 2017-02-21 2018-12-13 Etiquetado utilizando transposomas inmovilizados con ligadores. MX2022005777A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762461620P 2017-02-21 2017-02-21

Publications (1)

Publication Number Publication Date
MX2022005777A true MX2022005777A (es) 2022-06-09

Family

ID=61557365

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2018015529A MX392295B (es) 2017-02-21 2018-02-20 Etiquetado utilizando transposomas inmovilizados con ligadores
MX2022005777A MX2022005777A (es) 2017-02-21 2018-12-13 Etiquetado utilizando transposomas inmovilizados con ligadores.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2018015529A MX392295B (es) 2017-02-21 2018-02-20 Etiquetado utilizando transposomas inmovilizados con ligadores

Country Status (16)

Country Link
US (4) US10920219B2 (es)
EP (2) EP3452621B1 (es)
JP (3) JP7164276B2 (es)
KR (3) KR20250143875A (es)
CN (2) CN109415758B (es)
AU (2) AU2018225503B2 (es)
BR (1) BR112018076259A2 (es)
CA (1) CA3026206A1 (es)
DK (1) DK3452621T3 (es)
ES (1) ES2933806T3 (es)
FI (1) FI3452621T3 (es)
IL (3) IL303805B2 (es)
MX (2) MX392295B (es)
NZ (1) NZ748776A (es)
SG (1) SG11201810639RA (es)
WO (1) WO2018156519A1 (es)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
IL303805B2 (en) 2017-02-21 2024-08-01 Illumina Inc Tegumentation using fixed transpososomes with linkers
EP4345172A3 (en) 2017-06-05 2024-07-03 Becton, Dickinson and Company Sample indexing for single cells
WO2019104034A1 (en) 2017-11-21 2019-05-31 Arima Genomics, Inc. Preserving spatial-proximal contiguity and molecular contiguity in nucleic acid templates
JP7511344B2 (ja) 2018-04-20 2024-07-05 イルミナ インコーポレイテッド 単一細胞を封入する方法、封入された細胞およびその使用
US11773441B2 (en) * 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
CA3114732A1 (en) * 2019-01-11 2020-07-16 Illumina Cambridge Limited Complex surface-bound transposome complexes
TWI873119B (zh) 2019-01-29 2025-02-21 美商伊路米納有限公司 流通槽及製備其之方法
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
CA3128098A1 (en) 2019-02-14 2020-08-20 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized tn5 transposase
CN112888791A (zh) * 2019-04-29 2021-06-01 伊鲁米那股份有限公司 通过快速温育和核酸富集来鉴定和分析微生物样本
JP7603586B2 (ja) 2019-07-12 2024-12-20 イルミナ ケンブリッジ リミテッド 電気泳動を用いた核酸ライブラリの調製
CN110734908B (zh) * 2019-11-15 2021-06-08 福州福瑞医学检验实验室有限公司 高通量测序文库的构建方法以及用于文库构建的试剂盒
WO2021154648A2 (en) * 2020-01-27 2021-08-05 Illumina, Inc. Kit, system, and flow cell
MX2021015805A (es) * 2020-01-27 2022-04-01 Illumina Inc Kit, sistema, y celda de flujo.
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US20230105642A1 (en) * 2020-03-30 2023-04-06 Illumina, Inc. Method and compositions for preparing nucleic acid libraries
JP2023532905A (ja) * 2020-07-08 2023-08-01 イルミナ インコーポレイテッド トランスポソーム担体としてのビーズ
BR112023002051A2 (pt) * 2020-08-06 2023-04-18 Illumina Inc Preparação de bibliotecas de sequenciamento de rna e dna usando transpossomos ligados a microesferas
IL299783A (en) 2020-08-18 2023-03-01 Illumina Inc Sequence-Specific Targeted Transposition, Selection and Sorting of Nucleic Acids
CN112176421A (zh) * 2020-09-22 2021-01-05 上海英基生物科技有限公司 一种rna建库的方法
MX2023004461A (es) 2020-10-21 2023-05-03 Illumina Inc Plantillas de secuenciacion que comprenden multiples insertos y composiciones y metodos para mejorar la productividad de secuenciacion.
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
CA3214278A1 (en) 2021-03-29 2022-10-06 Illumina, Inc Improved methods of library preparation
BR112023019959A2 (pt) 2021-03-30 2024-01-30 Illumina Inc Métodos aprimorados de preparação isotérmica de biblioteca e dna complementar
JP2024511760A (ja) * 2021-03-31 2024-03-15 イルミナ インコーポレイテッド エラー補正のための固有分子識別子を有するトランスポゾンベースの技術を使用した指向性タグメンテーション配列決定ライブラリーの調製方法
US20240336913A1 (en) * 2021-06-01 2024-10-10 Derek BOGDANOFF Method for producing a population of symmetrically barcoded transposomes
WO2022256227A1 (en) * 2021-06-01 2022-12-08 The Regents Of The University Of California Methods for fragmenting complementary dna
AU2022355095A1 (en) * 2021-09-29 2024-04-11 Seqwell, Inc. Kits and methods for preparation of nucleic acid libraries for sequencing
CN114544925B (zh) * 2021-11-22 2025-11-14 浙江省农业科学院 一种利用CUT&Tag技术鉴定植物中转录因子与染色质互作的试剂盒及方法
JP2025500468A (ja) * 2021-12-23 2025-01-09 イルミナ インコーポレイテッド フローセル及び方法
CN116891532B (zh) * 2022-04-06 2025-02-07 南京诺唯赞生物科技股份有限公司 一种基于生物素:亲合素的二抗:转座酶复合物及其在检测蛋白质与dna相互作用中的应用
WO2023204594A1 (ko) * 2022-04-19 2023-10-26 서울대학교산학협력단 태그멘테이션을 이용한 벡터 삽입위치 검출 및 클론 정량 방법
WO2023225095A1 (en) * 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
US20250327064A1 (en) * 2022-05-26 2025-10-23 Illumina, Inc. Preparation of long read nucleic acid libraries
JPWO2024024583A1 (es) * 2022-07-25 2024-02-01
WO2024050304A1 (en) * 2022-08-29 2024-03-07 Illumina, Inc. Preparation and use of blocked substrates
WO2024254003A1 (en) 2023-06-05 2024-12-12 Illumina, Inc. Identification and mapping of methylation sites
WO2025128786A1 (en) * 2023-12-12 2025-06-19 Baylor College Of Medicine Methods for simultaneous characterization of genome and transcriptome of a single cell
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5964443A (en) 1997-08-13 1999-10-12 Cipa - Usa, Inc. Mounting bracket system for a rearview mirror
CN1367840A (zh) 1999-08-02 2002-09-04 威斯康星校友研究基金会 突变型Tn5转座酶及其使用方法
DE60131194T2 (de) 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire Sequenzbestimmung in echtzeit
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP3750020B2 (ja) 2000-12-07 2006-03-01 学校法人慶應義塾 Ｃ末端修飾タンパク質およびその製造方法、ならびに、ｃ末端修飾タンパク質の製造に用いる修飾剤および翻訳テンプレート、ならびに、ｃ末端修飾タンパク質を用いたタンパク質相互作用の検出方法
WO2003006625A2 (en) 2001-07-13 2003-01-23 Ambergen, Inc. Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
WO2004093645A2 (en) 2003-04-17 2004-11-04 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
JP2008513782A (ja) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 分子解析のための装置及び方法
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP3285067B1 (en) 2006-12-14 2022-06-22 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN102264914B (zh) 2008-10-24 2015-08-12 阿霹震中科技公司 用于修饰核酸的转座子末端组合物和方法
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
EP2458157B1 (fr) 2010-11-30 2015-10-14 Techspace Aero S.A. Abradable de virole intérieure de stator
US9238671B2 (en) 2011-01-28 2016-01-19 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
CN103443338B (zh) 2011-02-02 2017-09-22 华盛顿大学商业化中心 大规模平行邻接作图
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2013085918A1 (en) 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
NO2694769T3 (es) 2012-03-06 2018-03-03
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP3553175B1 (en) 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
EP3241913B1 (en) * 2013-07-03 2019-02-20 Illumina, Inc. System for sequencing by orthogonal synthesis
JP6626830B2 (ja) * 2013-11-07 2019-12-25 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． Ｄｎａ操作のための複数のトランスポザーゼアダプター
AU2014364926B2 (en) * 2013-12-20 2017-12-14 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
EP3102691B1 (en) 2014-02-03 2019-09-11 Thermo Fisher Scientific Baltics UAB Method for controlled dna fragmentation
TWI682997B (zh) 2014-04-15 2020-01-21 美商伊路米納有限公司 用於改善插入序列傾向及增加ｄｎａ輸入耐受度之經修飾的轉位酶
ES2713153T3 (es) * 2014-06-30 2019-05-20 Illumina Inc Métodos y composiciones que utilizan la transposición unilateral
CN107075513B (zh) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
DK3208335T3 (da) 2014-10-14 2020-08-31 Mgi Tech Co Ltd Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
JP7001475B2 (ja) * 2015-02-10 2022-01-19 イラミーナ インコーポレーテッド 細胞の構成成分を分析するための方法及び組成物
ES3039235T3 (en) * 2015-05-28 2025-10-20 Illumina Cambridge Ltd Surface-based tagmentation
IL303805B2 (en) 2017-02-21 2024-08-01 Illumina Inc Tegumentation using fixed transpososomes with linkers

Also Published As

Publication number Publication date
JP7164276B2 (ja) 2022-11-01
US20250333726A1 (en) 2025-10-30
IL311544A (en) 2024-05-01
JP2020510401A (ja) 2020-04-09
EP3452621A1 (en) 2019-03-13
IL303805B1 (en) 2024-04-01
IL263118A (en) 2018-12-31
AU2018225503B2 (en) 2024-07-18
CN109415758A (zh) 2019-03-01
BR112018076259A2 (pt) 2019-03-26
KR102866805B1 (ko) 2025-09-30
ES2933806T3 (es) 2023-02-14
FI3452621T3 (fi) 2022-12-15
MX392295B (es) 2025-03-24
SG11201810639RA (en) 2018-12-28
DK3452621T3 (da) 2022-12-12
US20210139887A1 (en) 2021-05-13
WO2018156519A1 (en) 2018-08-30
IL263118B2 (en) 2023-12-01
AU2018225503A9 (en) 2019-01-03
KR20190120056A (ko) 2019-10-23
CN115927538A (zh) 2023-04-07
IL303805A (en) 2023-08-01
US10920219B2 (en) 2021-02-16
US20180245069A1 (en) 2018-08-30
EP3783112A1 (en) 2021-02-24
US20230392142A1 (en) 2023-12-07
EP3452621B1 (en) 2022-09-21
IL303805B2 (en) 2024-08-01
JP2024133722A (ja) 2024-10-02
CA3026206A1 (en) 2018-08-30
KR20230165372A (ko) 2023-12-05
MX2018015529A (es) 2019-05-16
RU2018140894A3 (es) 2021-11-16
IL263118B1 (en) 2023-08-01
US12234451B2 (en) 2025-02-25
RU2018140894A (ru) 2020-05-20
AU2024227397A1 (en) 2025-01-09
JP2022177288A (ja) 2022-11-30
CN115927538B (zh) 2025-11-25
KR102607830B1 (ko) 2023-12-01
KR20250143875A (ko) 2025-10-02
AU2018225503A1 (en) 2018-12-13
NZ748776A (en) 2022-08-26
CN109415758B (zh) 2022-08-30
JP7630471B2 (ja) 2025-02-17
US11708573B2 (en) 2023-07-25

Similar Documents

Publication Publication Date Title
MX2022005777A (es) Etiquetado utilizando transposomas inmovilizados con ligadores.
MX2023008931A (es) Complejos de transposoma ligados a superficie de complejo.
WO2015160895A3 (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
PH12021551114A1 (en) Crispr-cas12j enzyme and system
SA523441205B1 (ar) التحرير المتعدد للجينوم
CA2884084C (en) Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
EP4269577A3 (en) Nucleobase editors and uses thereof
MX2017003847A (es) Proteinas biespecificas activables por proteasas.
CY1121657T1 (el) Μεθοδοι και συνθεσεις για κατευθυνομενη απο rna τροποποιηση dna στοχου και για κατευθυνομενη απο rna ρυθμιση της μεταγραφης
ES2721309T3 (es) Composición para preparar tagatosa y método para preparar tagatosa a partir de fructosa
WO2019139645A3 (en) High efficiency base editors comprising gam
MX391646B (es) Composiciones y métodos para la edición genética eficaz en e. coli usando sistemas de ácido desoxirribonucleico (arn) guía/endonucleasa de sistema asociado a repeticiones palindrómicas cortas agrupadas regularmente espaciadas (cas) en combinación con moldes de modificación de polinucleótido circular.
EP3942066A4 (en) NUCLEIC ACID HYBRIDIZATION PROCEDURE
EP3699280A3 (en) Novel cas9 systems and methods of use
MX391311B (es) Acidos nucleicos modificados dirigidos a acidos nucleicos.
MX2018000729A (es) Métodos para amplificar las secuencias de ácido nucleico.
SG10201903408VA (en) Contiguity preserving transposition
WO2018175258A8 (en) Methods and compositions for preparing nucleic acid libraries
EA201890565A1 (ru) Композиции и способы улучшения специфичности в геномной инженерии с применением рнк-направляемых эндонуклеаз
EP4520841A3 (en) Enzyme- and amplification-free sequencing
WO2014182528A3 (en) Multiplex labeling of molecules by sequential hybridization barcoding
WO2015035162A3 (en) Cas9 variants and uses thereof
EP4527942A3 (en) Preserving genomic connectivity information in fragmented genomic dna samples
WO2020030984A3 (en) Compositions and methods for genome engineering with cas12a proteins
MX2021001418A (es) Produccion de proteinas heteromultimericas usando celulas de mamifero.