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MX2023008931A - Complejos de transposoma ligados a superficie de complejo. - Google Patents

Complejos de transposoma ligados a superficie de complejo.

Info

Publication number
MX2023008931A
MX2023008931A MX2023008931A MX2023008931A MX2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A
Authority
MX
Mexico
Prior art keywords
transposome complexes
complex surface
complexes linked
compositions
methods
Prior art date
Application number
MX2023008931A
Other languages
English (en)
Inventor
Andrew Slatter
Esther Musgrave-Brown
Susan C Verity
Niall Gormley
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of MX2023008931A publication Critical patent/MX2023008931A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente descripción se relaciona con métodos, composiciones, y kits para generar una genoteca de fragmentos de ácido nucleico marcados sin usar la amplificación por PCR, que incluye métodos y composiciones para la fragmentación y marcación de ácidos nucleicos (p. ej., ADN) usando complejos de transposoma inmovilizados en soporte sólido.
MX2023008931A 2019-01-11 2021-03-26 Complejos de transposoma ligados a superficie de complejo. MX2023008931A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962791509P 2019-01-11 2019-01-11
US201962840610P 2019-04-30 2019-04-30

Publications (1)

Publication Number Publication Date
MX2023008931A true MX2023008931A (es) 2023-08-10

Family

ID=69172787

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2021003625A MX2021003625A (es) 2019-01-11 2020-01-10 Complejos de transposoma ligados a superficie de complejo.
MX2023008931A MX2023008931A (es) 2019-01-11 2021-03-26 Complejos de transposoma ligados a superficie de complejo.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2021003625A MX2021003625A (es) 2019-01-11 2020-01-10 Complejos de transposoma ligados a superficie de complejo.

Country Status (13)

Country Link
US (3) US11685946B2 (es)
EP (1) EP3908672A1 (es)
JP (2) JP7561120B2 (es)
KR (1) KR20210114918A (es)
CN (2) CN112930405B (es)
AU (1) AU2020206443A1 (es)
BR (1) BR112021006038A2 (es)
CA (1) CA3114732A1 (es)
IL (1) IL281664B2 (es)
MX (2) MX2021003625A (es)
MY (1) MY204975A (es)
SG (1) SG11202102918WA (es)
WO (1) WO2020144373A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
BR112023002051A2 (pt) * 2020-08-06 2023-04-18 Illumina Inc Preparação de bibliotecas de sequenciamento de rna e dna usando transpossomos ligados a microesferas
CA3191834A1 (en) * 2020-09-11 2022-03-17 Steven Henikoff Improved high efficiency targeted in situ genome-wide profiling
DK4288562T3 (da) * 2021-02-04 2025-02-10 Illumina Inc Indekseret long-read-generering på transposom-bundne beads
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
CA3214278A1 (en) * 2021-03-29 2022-10-06 Illumina, Inc Improved methods of library preparation
JP2024511760A (ja) 2021-03-31 2024-03-15 イルミナ インコーポレイテッド エラー補正のための固有分子識別子を有するトランスポゾンベースの技術を使用した指向性タグメンテーション配列決定ライブラリーの調製方法
KR102875170B1 (ko) * 2021-04-09 2025-10-30 주식회사 셀레믹스 간소화된 차세대 염기서열 분석 라이브러리 전처리 방법
AU2022355095A1 (en) * 2021-09-29 2024-04-11 Seqwell, Inc. Kits and methods for preparation of nucleic acid libraries for sequencing
WO2023225095A1 (en) * 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
JPWO2024024583A1 (es) * 2022-07-25 2024-02-01
CN118272508B (zh) * 2023-06-28 2024-11-08 浙江大学 基于单端接头转座酶的单细胞基因组测序试剂盒及其应用
WO2025117738A1 (en) 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
WO2025207886A1 (en) * 2024-03-28 2025-10-02 Illumina, Inc. Kits and methods for on-flow cell library preparation and methylation detection
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
DE60131194T2 (de) 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire Sequenzbestimmung in echtzeit
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
JP2008513782A (ja) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 分子解析のための装置及び方法
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN102264914B (zh) 2008-10-24 2015-08-12 阿霹震中科技公司 用于修饰核酸的转座子末端组合物和方法
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CN103443338B (zh) * 2011-02-02 2017-09-22 华盛顿大学商业化中心 大规模平行邻接作图
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP3553175B1 (en) * 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
WO2015026853A2 (en) 2013-08-19 2015-02-26 Abbott Molecular Inc. Next-generation sequencing libraries
JP6626830B2 (ja) 2013-11-07 2019-12-25 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． Ｄｎａ操作のための複数のトランスポザーゼアダプター
WO2015089339A2 (en) 2013-12-11 2015-06-18 Changping Shi Compositions, methods and kits for dna fragmentation and tagmentation
TWI682997B (zh) 2014-04-15 2020-01-21 美商伊路米納有限公司 用於改善插入序列傾向及增加ｄｎａ輸入耐受度之經修飾的轉位酶
WO2016037361A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 试剂盒及其在核酸测序中的用途
AU2015331739B2 (en) * 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
US10815478B2 (en) 2014-11-05 2020-10-27 Illumina, Inc. Method of sequential tagmentation with transposase compositions for reduction of insertion bias
ES3039235T3 (en) 2015-05-28 2025-10-20 Illumina Cambridge Ltd Surface-based tagmentation
US20170283864A1 (en) * 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
WO2018013710A1 (en) * 2016-07-12 2018-01-18 F. Hoffman-La Roche Ag Primer extension target enrichment
US10207332B2 (en) 2016-07-29 2019-02-19 Jtekt Corporation Work holder and work machining method
IL303805B2 (en) * 2017-02-21 2024-08-01 Illumina Inc Tegumentation using fixed transpososomes with linkers
CN110832087B (zh) 2017-05-08 2025-03-18 伊鲁米那股份有限公司 用于多核苷酸样品的索引的通用短衔接子
US20180340169A1 (en) * 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4180534A1 (en) * 2017-11-02 2023-05-17 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
AU2018366213B2 (en) * 2017-11-08 2025-05-15 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters

Also Published As

Publication number Publication date
MY204975A (en) 2024-09-25
CN112930405A (zh) 2021-06-08
SG11202102918WA (en) 2021-04-29
IL281664B1 (en) 2024-03-01
CN112930405B (zh) 2025-01-28
CA3114732A1 (en) 2020-07-16
CN119842869A (zh) 2025-04-18
US12448644B2 (en) 2025-10-21
WO2020144373A1 (en) 2020-07-16
AU2020206443A1 (en) 2021-04-15
US20210269856A1 (en) 2021-09-02
US11685946B2 (en) 2023-06-27
JP7561120B2 (ja) 2024-10-03
US20230340568A1 (en) 2023-10-26
JP2024170677A (ja) 2024-12-10
EP3908672A1 (en) 2021-11-17
US11306348B2 (en) 2022-04-19
MX2021003625A (es) 2021-05-27
IL281664A (en) 2021-05-31
BR112021006038A2 (pt) 2021-10-26
JP2022516821A (ja) 2022-03-03
KR20210114918A (ko) 2021-09-24
US20200224246A1 (en) 2020-07-16
IL281664B2 (en) 2024-07-01

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