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MX2019004629A - Metodo para preparar rebaudiosido c mediante el uso del metodo enzimatico. - Google Patents

Metodo para preparar rebaudiosido c mediante el uso del metodo enzimatico.

Info

Publication number
MX2019004629A
MX2019004629A MX2019004629A MX2019004629A MX2019004629A MX 2019004629 A MX2019004629 A MX 2019004629A MX 2019004629 A MX2019004629 A MX 2019004629A MX 2019004629 A MX2019004629 A MX 2019004629A MX 2019004629 A MX2019004629 A MX 2019004629A
Authority
MX
Mexico
Prior art keywords
enzymatic
rebaudioside
preparing rebaudioside
udp
substrate
Prior art date
Application number
MX2019004629A
Other languages
English (en)
Inventor
Tao Alex
Li Guoqing
Wang Wenxia
Zheng Leilei
Zhu Chunlei
Liang Xiaoliang
Chan Kuikiu
Original Assignee
Pepsico Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pepsico Inc filed Critical Pepsico Inc
Publication of MX2019004629A publication Critical patent/MX2019004629A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporciona un método para preparar Rebaudiósido C mediante el uso de un método enzimático, que comprende el uso de rubosósido o dulcósido A como un sustrato y haciendo que el sustrato, en presencia de un donante glicosilo, reaccione bajo la catálisis de la célula recombinante que contiene glicosiltransferasa UDP y/o glicosiltransferasa UDP preparadas de estas para generar Rebaudiósido C.
MX2019004629A 2016-10-21 2016-10-21 Metodo para preparar rebaudiosido c mediante el uso del metodo enzimatico. MX2019004629A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/102910 WO2018072203A1 (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙c的方法

Publications (1)

Publication Number Publication Date
MX2019004629A true MX2019004629A (es) 2019-06-06

Family

ID=62018093

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019004629A MX2019004629A (es) 2016-10-21 2016-10-21 Metodo para preparar rebaudiosido c mediante el uso del metodo enzimatico.

Country Status (8)

Country Link
US (2) US11312985B2 (es)
EP (1) EP3530746A4 (es)
JP (1) JP2019532650A (es)
CN (2) CN117051063A (es)
AU (1) AU2016427120B2 (es)
MX (1) MX2019004629A (es)
RU (1) RU2736155C1 (es)
WO (1) WO2018072203A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11359222B2 (en) 2016-10-21 2022-06-14 Pepsico, Inc. Enzymatic method for preparing Rebaudioside j
JP6972123B2 (ja) 2016-10-21 2021-11-24 ペプシコ・インク 酵素法を使用するレバウディオサイドｎを調製するための方法

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE547953T1 (de) 2007-01-22 2012-03-15 Cargill Inc Verfahren zur herstellung gereinigter rebaudiosid-a-zusammensetzungen durch solvent/antisolvent- kristallisation
CN101225424B (zh) 2007-09-13 2013-05-29 天津药物研究院 环黄芪醇的单葡萄糖苷、其制备方法、药物组合物和应用
CN102216313A (zh) 2008-10-03 2011-10-12 守田化学工业株式会社 新的甜菊醇糖苷
CA2775732C (en) 2009-10-15 2018-10-09 Purecircle Sdn Bhd High-purity rebaudioside d and applications
US8501261B2 (en) 2010-05-21 2013-08-06 Purecircle Sdn Bhd High-purity Rebaudioside C and process for purification of the same
AU2015261617C1 (en) * 2010-06-02 2019-01-31 Lallemand Bio-Ingredients Usa Llc Recombinant production of steviol glycosides
NZ604915A (en) * 2010-06-02 2014-10-31 Evolva Nutrition Inc Recombinant production of steviol glycosides
US8962698B2 (en) 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
CN103732753B (zh) 2011-08-08 2018-03-30 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN103031283B (zh) 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法
EP3345490B1 (en) 2011-12-19 2023-11-08 The Coca-Cola Company Beverage comprising rebaudioside x
WO2013110673A1 (en) 2012-01-23 2013-08-01 Dsm Ip Assets B.V. Diterpene production
CN110358795B (zh) 2012-05-22 2024-12-03 谱赛科有限责任公司 高纯度的甜菊醇糖苷
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
US20150342234A1 (en) * 2012-12-05 2015-12-03 Evolva Sa Steviol Glycoside Compositions Sensory Properties
CN103088041B (zh) 2013-01-29 2015-01-07 江南大学 一种可用于高效生产角质酶的角质酶基因及其应用
EP3892728A1 (en) 2013-02-06 2021-10-13 Evolva SA Methods for improved production of rebaudioside d and rebaudioside m
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
SG2013092820A (en) 2013-12-16 2015-07-30 Ngee Ann Polytechnic Xylose isomerase genes for xylose-fermenting yeast construction
CN103757074B (zh) * 2014-01-16 2015-12-02 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
US20170211113A1 (en) 2014-01-28 2017-07-27 Pepsico, Inc. Method for preparing rebaudioside m by using enzyme method
WO2016028899A1 (en) 2014-08-19 2016-02-25 The Coca-Cola Company Methods for preparing rebaudioside i and uses
EP4063513A1 (en) 2015-05-29 2022-09-28 Cargill, Incorporated Heat treatment to produce glycosides
CN105200098A (zh) 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法
US20190203244A1 (en) 2015-08-20 2019-07-04 Pepsico, Inc. Preparation of rebaudioside m in a single reaction vessel
US11359222B2 (en) 2016-10-21 2022-06-14 Pepsico, Inc. Enzymatic method for preparing Rebaudioside j
JP6972123B2 (ja) 2016-10-21 2021-11-24 ペプシコ・インク 酵素法を使用するレバウディオサイドｎを調製するための方法

Also Published As

Publication number Publication date
EP3530746A1 (en) 2019-08-28
JP2019532650A (ja) 2019-11-14
CN110199029A (zh) 2019-09-03
US20190367960A1 (en) 2019-12-05
CN117051063A (zh) 2023-11-14
EP3530746A4 (en) 2020-06-24
US11976312B2 (en) 2024-05-07
AU2016427120A1 (en) 2019-05-30
US20220220525A1 (en) 2022-07-14
AU2016427120A8 (en) 2019-06-06
US11312985B2 (en) 2022-04-26
WO2018072203A1 (zh) 2018-04-26
CA3041150A1 (en) 2018-04-26
CN110199029B (zh) 2023-09-05
AU2016427120B2 (en) 2022-08-18
RU2736155C1 (ru) 2020-11-12

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