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MX2024014263A - Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e - Google Patents

Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e

Info

Publication number
MX2024014263A
MX2024014263A MX2024014263A MX2024014263A MX2024014263A MX 2024014263 A MX2024014263 A MX 2024014263A MX 2024014263 A MX2024014263 A MX 2024014263A MX 2024014263 A MX2024014263 A MX 2024014263A MX 2024014263 A MX2024014263 A MX 2024014263A
Authority
MX
Mexico
Prior art keywords
rebaudioside
steviol glycoside
biosynthetic production
enzymes
aspects
Prior art date
Application number
MX2024014263A
Other languages
English (en)
Inventor
Guohong Mao
Jacob Edward Vick
Michael Batten
Yang Luo
Yilin Wu
Xiaodan Yu
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of MX2024014263A publication Critical patent/MX2024014263A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22035—Histolysain (3.4.22.35)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Seasonings (AREA)

Abstract

La presente invención se relaciona, en algunos aspectos, a la producción de rebaudiósido de glucósido de esteviol D4 mediante el uso de rebaudiósido E. En algunos aspectos, la invención se refiere a enzimas CP1 mutantes, enzimas HV1 mutantes, así como a células hospederas y a métodos que utilizan tales enzimas, tales como para producir rebaudiósido D4.
MX2024014263A 2017-03-06 2019-09-04 Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e MX2024014263A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762467467P 2017-03-06 2017-03-06
PCT/US2017/064430 WO2018164747A1 (en) 2017-03-06 2017-12-04 Biosynthetic production of steviol glycoside rebaudioside d4 from rebaudioside e

Publications (1)

Publication Number Publication Date
MX2024014263A true MX2024014263A (es) 2024-12-06

Family

ID=63448867

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019010537A MX2019010537A (es) 2017-03-06 2017-12-04 Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e.
MX2024014263A MX2024014263A (es) 2017-03-06 2019-09-04 Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2019010537A MX2019010537A (es) 2017-03-06 2017-12-04 Produccion biosintetica del rebaudiosido d4 de glucosido de esteviol a partir del rebaudiosido e.

Country Status (12)

Country Link
US (2) US11306340B2 (es)
EP (1) EP3592157A4 (es)
JP (2) JP7194111B2 (es)
KR (1) KR102554790B1 (es)
CN (1) CN110381751B (es)
AU (1) AU2017402700A1 (es)
BR (1) BR112019018361B1 (es)
CA (1) CA3055288A1 (es)
MX (2) MX2019010537A (es)
MY (1) MY202423A (es)
RU (1) RU2764803C2 (es)
WO (1) WO2018164747A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107105734A (zh) 2014-09-19 2017-08-29 谱赛科有限责任公司 高纯度甜菊醇糖苷
EP3277829B1 (en) 2015-04-03 2020-07-08 DSM IP Assets B.V. Steviol glycosides
MX2019004368A (es) 2016-10-14 2019-08-12 Conagen Inc Produccion biosintetica de glicosidos de esteviol y procesos de los mismos.
EP3644758A4 (en) 2017-06-30 2021-03-31 Conagen Inc. Hydrolysis of steviol glycosides by beta-glucosidase
EP3764810A4 (en) * 2018-03-16 2021-10-20 PureCircle USA Inc. HIGH PURITY STEVIOL GLYCOSIDES
CA3094205A1 (en) * 2018-03-16 2019-09-19 Purecircle Usa Inc. High-purity steviol glycosides
CN111424065B (zh) * 2020-03-24 2022-08-16 金禾益康(北京)生物科技有限公司 使用糖基转移酶对甜菊糖苷类化合物进行糖基化方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2708548T3 (pl) * 2009-10-15 2018-07-31 Purecircle Sdn Bhd Wysokiej czystości Rebaudiozyd D i zastosowania
CN108396044A (zh) * 2011-08-08 2018-08-14 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN102766667B (zh) * 2012-08-14 2014-06-11 成都南诺格生物科技有限责任公司 甜菊苷转化为莱鲍迪苷e的方法
US9611498B2 (en) * 2012-09-29 2017-04-04 Shanghai Institutes For Biological Sciences, Chinese Academy Of Sciences Method for producing stevioside compounds by microorganism
CN103114099B (zh) * 2013-02-07 2014-06-11 广西大学 编码糖基水解酶家族1的β-葡萄糖苷酶基因及其应用
CN103397064B (zh) * 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
ES2709439T3 (es) * 2013-11-01 2019-04-16 Conagen Inc Producción recombinante de glucósidos de esteviol
US9522929B2 (en) * 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
CN107105734A (zh) * 2014-09-19 2017-08-29 谱赛科有限责任公司 高纯度甜菊醇糖苷
MY182396A (en) * 2014-11-05 2021-01-23 Manus Biosynthesis Inc Microbial production of steviol glycosides
MX2019004368A (es) * 2016-10-14 2019-08-12 Conagen Inc Produccion biosintetica de glicosidos de esteviol y procesos de los mismos.
AU2018215335B2 (en) * 2017-02-03 2020-10-01 Tate & Lyle Solutions Usa Llc Engineered glycosyltransferases and steviol glycoside glucosylation methods

Also Published As

Publication number Publication date
RU2019130032A3 (es) 2021-08-06
WO2018164747A1 (en) 2018-09-13
AU2017402700A1 (en) 2019-10-17
BR112019018361B1 (pt) 2023-02-14
JP7194111B2 (ja) 2022-12-21
JP2023036665A (ja) 2023-03-14
EP3592157A1 (en) 2020-01-15
CN110381751B (zh) 2023-09-05
US11306340B2 (en) 2022-04-19
EP3592157A4 (en) 2021-01-13
MX2019010537A (es) 2019-11-21
BR112019018361A2 (pt) 2020-04-07
US20220282297A1 (en) 2022-09-08
CA3055288A1 (en) 2018-09-13
KR20190125330A (ko) 2019-11-06
US20200102588A1 (en) 2020-04-02
KR102554790B1 (ko) 2023-07-13
JP2020511132A (ja) 2020-04-16
MY202423A (en) 2024-04-28
RU2764803C2 (ru) 2022-01-21
RU2019130032A (ru) 2021-04-07
CN110381751A (zh) 2019-10-25

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