MX2019004628A - Metodo para preparar rebaudiosido n usando el metodo enzimatico. - Google Patents
Metodo para preparar rebaudiosido n usando el metodo enzimatico.Info
- Publication number
- MX2019004628A MX2019004628A MX2019004628A MX2019004628A MX2019004628A MX 2019004628 A MX2019004628 A MX 2019004628A MX 2019004628 A MX2019004628 A MX 2019004628A MX 2019004628 A MX2019004628 A MX 2019004628A MX 2019004628 A MX2019004628 A MX 2019004628A
- Authority
- MX
- Mexico
- Prior art keywords
- rebaudioside
- enzymatic
- preparing rebaudioside
- preparing
- glycosyltransferase
- Prior art date
Links
- 229930188195 rebaudioside Natural products 0.000 title abstract 3
- 238000006911 enzymatic reaction Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 239000001512 FEMA 4601 Substances 0.000 abstract 1
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 abstract 1
- HINSNOJRHFIMKB-DJDMUFINSA-N [(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl] (1R,4S,5R,9S,10R,13S)-13-[(2S,3R,4S,5R,6R)-5-hydroxy-6-(hydroxymethyl)-3,4-bis[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]oxan-2-yl]oxy-5,9-dimethyl-14-methylidenetetracyclo[11.2.1.01,10.04,9]hexadecane-5-carboxylate Chemical compound [H][C@@]1(O[C@@H]2[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]2OC(=O)[C@]2(C)CCC[C@@]3(C)[C@]4([H])CC[C@@]5(C[C@]4(CC5=C)CC[C@]23[H])O[C@]2([H])O[C@H](CO)[C@@H](O)[C@H](O[C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O[C@]2([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O HINSNOJRHFIMKB-DJDMUFINSA-N 0.000 abstract 1
- 238000006555 catalytic reaction Methods 0.000 abstract 1
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 abstract 1
- 239000000348 glycosyl donor Substances 0.000 abstract 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 abstract 1
- 235000019203 rebaudioside A Nutrition 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/08—Dextran
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se proporciona un método para preparar rebaudiósido N usando un método enzimático, que comprende el uso de rebaudiósido A o rebaudiósido J como un sustrato, y hacer que el substrato, en la presencia de un donante de glicosilo, reaccione bajo la catálisis de una UDP-glicosiltransferasa y/o una célula recombinante que contiene UDP-glicosiltransferasa para generar rebaudiósido N.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2016/102948 WO2018072213A1 (zh) | 2016-10-21 | 2016-10-21 | 一种酶法制备瑞鲍迪甙n的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2019004628A true MX2019004628A (es) | 2019-06-06 |
Family
ID=62018053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019004628A MX2019004628A (es) | 2016-10-21 | 2016-10-21 | Metodo para preparar rebaudiosido n usando el metodo enzimatico. |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11352653B2 (es) |
| EP (1) | EP3530745A4 (es) |
| JP (1) | JP6972123B2 (es) |
| CN (2) | CN109890973B (es) |
| AU (1) | AU2016427130B2 (es) |
| BR (1) | BR112019008048A2 (es) |
| CA (1) | CA3041152A1 (es) |
| MX (1) | MX2019004628A (es) |
| RU (1) | RU2737118C2 (es) |
| WO (1) | WO2018072213A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11359222B2 (en) | 2016-10-21 | 2022-06-14 | Pepsico, Inc. | Enzymatic method for preparing Rebaudioside j |
| WO2018072203A1 (zh) | 2016-10-21 | 2018-04-26 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙c的方法 |
| US20210246156A1 (en) * | 2018-06-08 | 2021-08-12 | Purecircle Usa, Inc. | High-purity steviol glycosides |
| JP7318989B2 (ja) * | 2019-03-29 | 2023-08-01 | コナゲン インコーポレイテッド | Ｕｄｐ－ラムノースの生合成生産 |
| EP4086237B1 (en) | 2021-05-05 | 2025-08-06 | Hypex Bio Explosives Technology AB | Composition for forming a hydrogen peroxide based emulsion explosive |
| KR102628374B1 (ko) * | 2021-10-19 | 2024-01-24 | 씨제이제일제당 주식회사 | 신규한 당전이효소 및 이의 용도 |
| CN116813679A (zh) * | 2023-06-25 | 2023-09-29 | 东台市浩瑞生物科技有限公司 | 一种甜菊糖苷rn晶型h1及其制备方法和用途 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE547953T1 (de) | 2007-01-22 | 2012-03-15 | Cargill Inc | Verfahren zur herstellung gereinigter rebaudiosid-a-zusammensetzungen durch solvent/antisolvent- kristallisation |
| CN101225424B (zh) | 2007-09-13 | 2013-05-29 | 天津药物研究院 | 环黄芪醇的单葡萄糖苷、其制备方法、药物组合物和应用 |
| CN102216313A (zh) | 2008-10-03 | 2011-10-12 | 守田化学工业株式会社 | 新的甜菊醇糖苷 |
| CA2775732C (en) | 2009-10-15 | 2018-10-09 | Purecircle Sdn Bhd | High-purity rebaudioside d and applications |
| NZ604915A (en) | 2010-06-02 | 2014-10-31 | Evolva Nutrition Inc | Recombinant production of steviol glycosides |
| US8962698B2 (en) | 2011-01-28 | 2015-02-24 | Tate & Lyle Ingredients Americas Llc | Rebaudioside-mogroside V blends |
| CN103732753B (zh) | 2011-08-08 | 2018-03-30 | 埃沃尔瓦公司 | 甜菊醇糖苷类的重组生产 |
| CN103031283B (zh) | 2011-10-08 | 2015-07-08 | 成都华高瑞甜科技有限公司 | 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法 |
| CN103159808B (zh) * | 2011-12-09 | 2017-03-29 | 上海泓博智源医药股份有限公司 | 一种制备天然甜味剂的工艺方法 |
| EP3345490B1 (en) | 2011-12-19 | 2023-11-08 | The Coca-Cola Company | Beverage comprising rebaudioside x |
| WO2013110673A1 (en) | 2012-01-23 | 2013-08-01 | Dsm Ip Assets B.V. | Diterpene production |
| CN110358795B (zh) * | 2012-05-22 | 2024-12-03 | 谱赛科有限责任公司 | 高纯度的甜菊醇糖苷 |
| US9752174B2 (en) | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| US20150342234A1 (en) | 2012-12-05 | 2015-12-03 | Evolva Sa | Steviol Glycoside Compositions Sensory Properties |
| CN103088041B (zh) | 2013-01-29 | 2015-01-07 | 江南大学 | 一种可用于高效生产角质酶的角质酶基因及其应用 |
| EP3892728A1 (en) | 2013-02-06 | 2021-10-13 | Evolva SA | Methods for improved production of rebaudioside d and rebaudioside m |
| US9717267B2 (en) | 2013-03-14 | 2017-08-01 | The Coca-Cola Company | Beverages containing rare sugars |
| CN103397064B (zh) | 2013-08-14 | 2015-04-15 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙m的方法 |
| AU2014306548B2 (en) * | 2013-08-15 | 2018-02-22 | Cargill, Incorporated | Sweetener and sweetened compositions incorporating Rebaudoside N |
| RU2741103C2 (ru) | 2013-11-01 | 2021-01-22 | Конаджен Инк. | Рекомбинантное получение стевиол-гликозидов |
| SG2013092820A (en) | 2013-12-16 | 2015-07-30 | Ngee Ann Polytechnic | Xylose isomerase genes for xylose-fermenting yeast construction |
| CN103757074B (zh) | 2014-01-16 | 2015-12-02 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙m的方法 |
| US20170211113A1 (en) | 2014-01-28 | 2017-07-27 | Pepsico, Inc. | Method for preparing rebaudioside m by using enzyme method |
| US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
| WO2016028899A1 (en) | 2014-08-19 | 2016-02-25 | The Coca-Cola Company | Methods for preparing rebaudioside i and uses |
| CN106714578B (zh) | 2014-10-03 | 2020-07-28 | 康纳根有限公司 | 无热量甜味剂及合成方法 |
| JP2018503388A (ja) | 2015-01-30 | 2018-02-08 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | 組み換え宿主におけるステビオールグリコシドの生産 |
| EP4063513A1 (en) | 2015-05-29 | 2022-09-28 | Cargill, Incorporated | Heat treatment to produce glycosides |
| CN105200098A (zh) | 2015-06-30 | 2015-12-30 | 苏州汉酶生物技术有限公司 | 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法 |
| US20190203244A1 (en) | 2015-08-20 | 2019-07-04 | Pepsico, Inc. | Preparation of rebaudioside m in a single reaction vessel |
| US11359222B2 (en) | 2016-10-21 | 2022-06-14 | Pepsico, Inc. | Enzymatic method for preparing Rebaudioside j |
| WO2018072203A1 (zh) | 2016-10-21 | 2018-04-26 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙c的方法 |
-
2016
- 2016-10-21 JP JP2019520995A patent/JP6972123B2/ja active Active
- 2016-10-21 CN CN201680089986.1A patent/CN109890973B/zh active Active
- 2016-10-21 US US16/343,335 patent/US11352653B2/en active Active
- 2016-10-21 EP EP16919469.3A patent/EP3530745A4/en active Pending
- 2016-10-21 CA CA3041152A patent/CA3041152A1/en active Pending
- 2016-10-21 MX MX2019004628A patent/MX2019004628A/es unknown
- 2016-10-21 BR BR112019008048A patent/BR112019008048A2/pt not_active Application Discontinuation
- 2016-10-21 CN CN202310412340.4A patent/CN116515929A/zh active Pending
- 2016-10-21 RU RU2019112909A patent/RU2737118C2/ru active
- 2016-10-21 AU AU2016427130A patent/AU2016427130B2/en active Active
- 2016-10-21 WO PCT/CN2016/102948 patent/WO2018072213A1/zh not_active Ceased
-
2022
- 2022-05-16 US US17/663,631 patent/US11976313B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112019008048A2 (pt) | 2019-07-02 |
| AU2016427130B2 (en) | 2022-08-18 |
| CN109890973B (zh) | 2023-05-23 |
| JP2019531083A (ja) | 2019-10-31 |
| EP3530745A1 (en) | 2019-08-28 |
| AU2016427130A1 (en) | 2019-05-30 |
| CN109890973A (zh) | 2019-06-14 |
| RU2019112909A (ru) | 2020-11-23 |
| WO2018072213A1 (zh) | 2018-04-26 |
| CA3041152A1 (en) | 2018-04-26 |
| EP3530745A4 (en) | 2020-07-22 |
| US20220275415A1 (en) | 2022-09-01 |
| JP6972123B2 (ja) | 2021-11-24 |
| US11976313B2 (en) | 2024-05-07 |
| US20210214761A1 (en) | 2021-07-15 |
| CN116515929A (zh) | 2023-08-01 |
| RU2019112909A3 (es) | 2020-11-23 |
| RU2737118C2 (ru) | 2020-11-24 |
| US11352653B2 (en) | 2022-06-07 |
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