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MX2017011681A - Metodo para la sintesis microbiana de novo de terpenos. - Google Patents

Metodo para la sintesis microbiana de novo de terpenos.

Info

Publication number
MX2017011681A
MX2017011681A MX2017011681A MX2017011681A MX2017011681A MX 2017011681 A MX2017011681 A MX 2017011681A MX 2017011681 A MX2017011681 A MX 2017011681A MX 2017011681 A MX2017011681 A MX 2017011681A MX 2017011681 A MX2017011681 A MX 2017011681A
Authority
MX
Mexico
Prior art keywords
terpenes
heterologous
relates
microbial production
polypeptide
Prior art date
Application number
MX2017011681A
Other languages
English (en)
Inventor
Pelzer Ralf
Schrader Jens
Buchhaupt Markus
Sonntag Frank
KRONER Cora
Brueser Heike
Schroeder Hartwig
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of MX2017011681A publication Critical patent/MX2017011681A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/0301—Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/0101—(2E,6E)-Farnesyl diphosphate synthase (2.5.1.10), i.e. geranyltranstransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01036—Mevalonate kinase (2.7.1.36)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04002—Phosphomevalonate kinase (2.7.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01033—Diphosphomevalonate decarboxylase (4.1.1.33), i.e. mevalonate-pyrophosphate decarboxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03104—Alpha-humulene synthase (4.2.3.104)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/03—Intramolecular oxidoreductases (5.3) transposing C=C bonds (5.3.3)
    • C12Y503/03002—Isopentenyl-diphosphate DELTA-isomerase (5.3.3.2)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invención se refiere a la producción microbiana de terpenos. Los métodos conocidos para la producción microbiana de terpenos se realizan principalmente en función de la conversión directa de azúcares. Por lo tanto, los sustratos alternativos, en particular, las fuentes de carbono alternativas para su uso en la producción microbiana de terpenos eran deseables. La invención se refiere a una bacteria metilotrófica que contiene ADN recombinante que codifica al menos un polipéptido con actividad enzimática para la expresión heteróloga en dicha bacteria, en donde al menos dicho polipéptido con actividad enzimática se selecciona del grupo que consiste en una enzima de una vía del mevalonato heteróloga, una terpeno sintasa heteróloga y, en forma opcional, una sintasa heteróloga de un precursor de prenil difosfato. La invención se refiere además, en particular, a un método para la síntesis microbiana de novo de sesquiterpenos o diterpenos de metanol y/o etanol.
MX2017011681A 2015-03-11 2016-03-11 Metodo para la sintesis microbiana de novo de terpenos. MX2017011681A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102015103608.8A DE102015103608A1 (de) 2015-03-11 2015-03-11 Verfahren zur mikrobiellen de novo Synthese von Terpenen
PCT/EP2016/055239 WO2016142503A1 (de) 2015-03-11 2016-03-11 Verfahren zur mikrobiellen de novo synthese von terpenen

Publications (1)

Publication Number Publication Date
MX2017011681A true MX2017011681A (es) 2017-11-10

Family

ID=55538208

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017011681A MX2017011681A (es) 2015-03-11 2016-03-11 Metodo para la sintesis microbiana de novo de terpenos.

Country Status (7)

Country Link
US (1) US20180105838A1 (es)
EP (1) EP3268467A1 (es)
JP (1) JP2018507698A (es)
CN (1) CN107429223A (es)
DE (1) DE102015103608A1 (es)
MX (1) MX2017011681A (es)
WO (1) WO2016142503A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6741687B2 (ja) 2015-05-04 2020-08-19 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ メロナールの調製方法
BR112018011459A2 (pt) 2015-12-08 2018-11-27 Basf Se processo, material zeolítico, e, uso de um material zeolítico.
WO2017140909A1 (de) 2016-02-19 2017-08-24 Basf Se Enzymatische zyklisierung von homofarnesylsäure
US10981885B2 (en) 2016-05-31 2021-04-20 Basf Se Tetrahydropyranyl lower alkyl esters and the production of same using a ketene compound
CN108949788B (zh) * 2018-07-10 2020-07-14 广东省微生物研究所（广东省微生物分析检测中心） 番茄红素合成相关基因及其应用
CN109852600B (zh) * 2018-12-25 2021-12-31 天津大学 一种小萼苔倍半萜合成酶MTb及其基因序列
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用
CN112175891B (zh) * 2019-11-12 2023-04-21 中国科学院青岛生物能源与过程研究所 一种生产海藻糖的基因工程菌及其构建方法与应用
CN112852650B (zh) * 2019-11-27 2023-04-14 暨南大学 一种高产檀香烯和檀香醇的酿酒酵母工程菌及其构建方法与应用
CN112592843B (zh) * 2020-12-25 2022-06-14 南京师范大学 一种生产α-蛇麻烯的重组解脂耶氏酵母菌及其构建方法和应用
CN112941063B (zh) * 2021-04-22 2022-08-05 杭州师范大学 一种α-檀香烯合成酶、基因及应用
CN114181964B (zh) * 2021-11-02 2023-06-09 云南大学 一种表达盒组合、重组载体和重组酿酒酵母及其应用
CN116083462B (zh) * 2021-11-05 2024-11-15 武汉大学 一种萜类合酶基因及其表达盒、表达载体和重组微生物菌株
CN117165502A (zh) * 2022-05-26 2023-12-05 牛津大学(苏州)科技有限公司 一种合成萜类化合物的共培养系统和方法
WO2025102880A1 (en) * 2023-11-16 2025-05-22 Hong Kong Baptist University Method to produce unusual monoterpenoid and plant transformation vector

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7659097B2 (en) 2006-05-26 2010-02-09 Amyris Biotechnologies, Inc. Production of isoprenoids
EP3020799B1 (en) * 2006-09-26 2020-08-26 The Regents of The University of California Production of isoprenoids and isoprenoid precursors
SG194691A1 (en) * 2011-04-29 2013-12-30 Danisco Us Inc Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities
AU2012335091B2 (en) * 2011-11-09 2016-06-16 Amyris, Inc. Production of acetyl-coenzyme a derived isoprenoids
US20130323820A1 (en) * 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
EP2875136B1 (en) 2012-07-18 2017-04-12 Isobionics B.V. Rhodobacter for preparing terpenoids
WO2014089436A1 (en) * 2012-12-07 2014-06-12 Ginkgo Bioworks, Inc. Methods and systems for methylotrophic production of organic compounds
KR20150100666A (ko) * 2012-12-27 2015-09-02 세키스이가가쿠 고교가부시키가이샤 재조합 세포, 및 이소프렌의 생산 방법
WO2014138419A1 (en) * 2013-03-07 2014-09-12 Calysta Energy, Inc. Compositions and methods for biological production of isoprene
CN104120141A (zh) * 2014-07-14 2014-10-29 青岛农业大学 一种微生物催化合成β-石竹烯的方法及能够合成β-石竹烯的重组细胞

Also Published As

Publication number Publication date
US20180105838A1 (en) 2018-04-19
EP3268467A1 (de) 2018-01-17
CN107429223A (zh) 2017-12-01
JP2018507698A (ja) 2018-03-22
WO2016142503A1 (de) 2016-09-15
DE102015103608A1 (de) 2016-09-15

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