MX2016010717A - Métodos y composiciones para el perfilado de adn. - Google Patents
Métodos y composiciones para el perfilado de adn.Info
- Publication number
- MX2016010717A MX2016010717A MX2016010717A MX2016010717A MX2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- acid sample
- target sequence
- primers
- amplification
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 239000003550 marker Substances 0.000 abstract 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
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- C12Q2600/00—Oligonucleotides characterized by their use
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- Physics & Mathematics (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Abstract
Las realizaciones desveladas en el presente documento proporcionan métodos para construir un perfil de ADN que comprende: una muestra de ácido nucleico, la amplificación de la muestra de ácido nucleico con una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana que comprende un polimorfismo mononucleotídico (SNP) y al menos una secuencia diana que comprende una repetición en tándem, y determinar los genotipos de dicho al menos un SNP y de dicha al menos una repetición en tándem en los productos de amplificación, construyéndose de esta manera el perfil de ADN de la muestra de ácido nucleico. Las realizaciones desveladas en el presente documento proporcionan además, una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana corta y al menos una secuencia diana larga en una muestra de ácido nucleico, en la que la amplificación de la muestra de ácido nucleico usando la pluralidad de cebadores en una sola reacción da como resultado al menos un producto de amplificación corto y al menos un producto de amplificación largo, en la que cada uno de la pluralidad de cebadores comprende una o más secuencias marcadoras.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461940942P | 2014-02-18 | 2014-02-18 | |
| US201462043060P | 2014-08-28 | 2014-08-28 | |
| US201562103524P | 2015-01-14 | 2015-01-14 | |
| PCT/US2015/015939 WO2015126766A1 (en) | 2014-02-18 | 2015-02-13 | Methods and compositions for dna profiling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016010717A true MX2016010717A (es) | 2017-04-27 |
| MX379233B MX379233B (es) | 2025-03-10 |
Family
ID=52629678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016010717A MX379233B (es) | 2014-02-18 | 2015-02-13 | Métodos y composiciones para el perfilado de adn. |
Country Status (22)
| Country | Link |
|---|---|
| US (2) | US10422002B2 (es) |
| EP (2) | EP3108009B1 (es) |
| JP (3) | JP7011392B2 (es) |
| CN (1) | CN106164298B (es) |
| AU (2) | AU2015219289B2 (es) |
| BR (2) | BR112016019267B8 (es) |
| CA (1) | CA2940048C (es) |
| DK (1) | DK3108009T3 (es) |
| ES (1) | ES2866044T3 (es) |
| HR (1) | HRP20210953T1 (es) |
| HU (1) | HUE055256T2 (es) |
| LT (1) | LT3108009T (es) |
| MX (1) | MX379233B (es) |
| MY (2) | MY200537A (es) |
| NZ (1) | NZ723399A (es) |
| PL (1) | PL3108009T3 (es) |
| PT (1) | PT3108009T (es) |
| RS (1) | RS61976B1 (es) |
| RU (2) | RU2708337C2 (es) |
| SA (1) | SA516371691B1 (es) |
| SI (1) | SI3108009T1 (es) |
| WO (1) | WO2015126766A1 (es) |
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| JP6382935B2 (ja) * | 2013-03-15 | 2018-08-29 | アッヴィ・ドイチュラント・ゲー・エム・ベー・ハー・ウント・コー・カー・ゲー | 抗egfr抗体薬物複合体製剤 |
| CN106164298B (zh) * | 2014-02-18 | 2020-02-21 | 伊鲁米那股份有限公司 | 用于dna谱系分析的方法及组合物 |
| WO2016100049A1 (en) | 2014-12-18 | 2016-06-23 | Edico Genome Corporation | Chemically-sensitive field effect transistor |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| JP6966681B2 (ja) * | 2015-04-24 | 2021-11-17 | アティラ バイオシステムズ インコーポレイテッドAtila Biosystems Incorporated | 限られたヌクレオチド組成を有するプライマーを用いた増幅 |
| CN105755129B (zh) * | 2016-03-21 | 2020-03-31 | 北京市理化分析测试中心 | 基于新一代测序的基因座d8s1179的str分型方法 |
| WO2017196720A1 (en) * | 2016-05-13 | 2017-11-16 | Colorado State University Research Foundation | High throughput method to genotype plants |
| US10811539B2 (en) | 2016-05-16 | 2020-10-20 | Nanomedical Diagnostics, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10822647B2 (en) * | 2016-07-12 | 2020-11-03 | Biodynamics S.R.L. | Methods for using long ssDNA polynucleotides as primers (superprimers) in PCR assays |
| ES2870639T3 (es) | 2016-10-24 | 2021-10-27 | Geneinfosec Inc | Ocultación de información presente en los ácidos nucleicos |
| CN118638898A (zh) * | 2017-03-23 | 2024-09-13 | 华盛顿大学 | 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用 |
| CN106835292B (zh) * | 2017-04-05 | 2019-04-09 | 北京泛生子基因科技有限公司 | 一步法快速构建扩增子文库的方法 |
| EP3619325B1 (en) | 2017-05-05 | 2024-01-24 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
| WO2018217912A1 (en) * | 2017-05-23 | 2018-11-29 | President And Fellows Of Harvard College | Multiplex end-tagging amplification of nucleic acids |
| EP3642362B1 (en) * | 2017-06-20 | 2025-10-15 | Illumina, Inc. | Methods for addressing inefficiencies in amplification reactions |
| CN111183229A (zh) | 2017-08-11 | 2020-05-19 | 阿提拉生物系统股份有限公司 | 使用有限核苷酸组成的引物的数字扩增 |
| CN107868837B (zh) * | 2017-12-12 | 2019-03-01 | 苏州普瑞森基因科技有限公司 | 一种用于分析肠道微生物的引物组合物及其应用 |
| CN109988826A (zh) * | 2017-12-30 | 2019-07-09 | 安诺优达基因科技(北京)有限公司 | 一种基于str计算混合样本嵌合比例的方法以及系统 |
| EP3517629A1 (en) * | 2018-01-30 | 2019-07-31 | Myway Genetics S.r.L. | Use of cfdna fragments as biomarkers in patients after organ transplantation |
| CN108342469A (zh) * | 2018-02-27 | 2018-07-31 | 广州中安基因科技有限公司 | 一种皮肤基因检测的基因芯片 |
| AU2019240244A1 (en) * | 2018-03-22 | 2020-11-19 | President And Fellows Of Harvard College | Methods and compositions for molecular authentication |
| JP6646120B1 (ja) * | 2018-10-01 | 2020-02-14 | 日本ソフトウェアマネジメント株式会社 | 分解されたdnaで個体識別可能なdna鑑定方法 |
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| CN111485024B (zh) * | 2019-01-29 | 2024-04-26 | 深圳华大法医科技有限公司 | 用于个体特征确认的引物组合、及其应用 |
| KR102351579B1 (ko) * | 2019-06-25 | 2022-01-17 | 대한민국 | 상염색체 str 좌위를 포함한 ngs 패널 및 다중증폭 시스템 |
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