[go: up one dir, main page]

MX2016010717A - Métodos y composiciones para el perfilado de adn. - Google Patents

Métodos y composiciones para el perfilado de adn.

Info

Publication number
MX2016010717A
MX2016010717A MX2016010717A MX2016010717A MX2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A
Authority
MX
Mexico
Prior art keywords
nucleic acid
acid sample
target sequence
primers
amplification
Prior art date
Application number
MX2016010717A
Other languages
English (en)
Other versions
MX379233B (es
Inventor
M Stephens Kathryn
Holt Cydne
davis Carey
Jager Anne
Han Yonmee
Silva David
Jui Richard Shen Min-
AMINI Sasan
Steemers Frank
Walichiewicz Paulina
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2016010717A publication Critical patent/MX2016010717A/es
Publication of MX379233B publication Critical patent/MX379233B/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10Nucleic acid binding proteins
    • C12Q2522/101Single or double stranded nucleic acid binding proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/155Modifications characterised by incorporating/generating a new priming site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/204Modifications characterised by specific length of the oligonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Las realizaciones desveladas en el presente documento proporcionan métodos para construir un perfil de ADN que comprende: una muestra de ácido nucleico, la amplificación de la muestra de ácido nucleico con una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana que comprende un polimorfismo mononucleotídico (SNP) y al menos una secuencia diana que comprende una repetición en tándem, y determinar los genotipos de dicho al menos un SNP y de dicha al menos una repetición en tándem en los productos de amplificación, construyéndose de esta manera el perfil de ADN de la muestra de ácido nucleico. Las realizaciones desveladas en el presente documento proporcionan además, una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana corta y al menos una secuencia diana larga en una muestra de ácido nucleico, en la que la amplificación de la muestra de ácido nucleico usando la pluralidad de cebadores en una sola reacción da como resultado al menos un producto de amplificación corto y al menos un producto de amplificación largo, en la que cada uno de la pluralidad de cebadores comprende una o más secuencias marcadoras.
MX2016010717A 2014-02-18 2015-02-13 Métodos y composiciones para el perfilado de adn. MX379233B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461940942P 2014-02-18 2014-02-18
US201462043060P 2014-08-28 2014-08-28
US201562103524P 2015-01-14 2015-01-14
PCT/US2015/015939 WO2015126766A1 (en) 2014-02-18 2015-02-13 Methods and compositions for dna profiling

Publications (2)

Publication Number Publication Date
MX2016010717A true MX2016010717A (es) 2017-04-27
MX379233B MX379233B (es) 2025-03-10

Family

ID=52629678

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016010717A MX379233B (es) 2014-02-18 2015-02-13 Métodos y composiciones para el perfilado de adn.

Country Status (22)

Country Link
US (2) US10422002B2 (es)
EP (2) EP3108009B1 (es)
JP (3) JP7011392B2 (es)
CN (1) CN106164298B (es)
AU (2) AU2015219289B2 (es)
BR (2) BR112016019267B8 (es)
CA (1) CA2940048C (es)
DK (1) DK3108009T3 (es)
ES (1) ES2866044T3 (es)
HR (1) HRP20210953T1 (es)
HU (1) HUE055256T2 (es)
LT (1) LT3108009T (es)
MX (1) MX379233B (es)
MY (2) MY200537A (es)
NZ (1) NZ723399A (es)
PL (1) PL3108009T3 (es)
PT (1) PT3108009T (es)
RS (1) RS61976B1 (es)
RU (2) RU2708337C2 (es)
SA (1) SA516371691B1 (es)
SI (1) SI3108009T1 (es)
WO (1) WO2015126766A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6382935B2 (ja) * 2013-03-15 2018-08-29 アッヴィ・ドイチュラント・ゲー・エム・ベー・ハー・ウント・コー・カー・ゲー 抗egfr抗体薬物複合体製剤
CN106164298B (zh) * 2014-02-18 2020-02-21 伊鲁米那股份有限公司 用于dna谱系分析的方法及组合物
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
JP6966681B2 (ja) * 2015-04-24 2021-11-17 アティラ バイオシステムズ インコーポレイテッドAtila Biosystems Incorporated 限られたヌクレオチド組成を有するプライマーを用いた増幅
CN105755129B (zh) * 2016-03-21 2020-03-31 北京市理化分析测试中心 基于新一代测序的基因座d8s1179的str分型方法
WO2017196720A1 (en) * 2016-05-13 2017-11-16 Colorado State University Research Foundation High throughput method to genotype plants
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10822647B2 (en) * 2016-07-12 2020-11-03 Biodynamics S.R.L. Methods for using long ssDNA polynucleotides as primers (superprimers) in PCR assays
ES2870639T3 (es) 2016-10-24 2021-10-27 Geneinfosec Inc Ocultación de información presente en los ácidos nucleicos
CN118638898A (zh) * 2017-03-23 2024-09-13 华盛顿大学 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用
CN106835292B (zh) * 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
EP3619325B1 (en) 2017-05-05 2024-01-24 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
WO2018217912A1 (en) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
EP3642362B1 (en) * 2017-06-20 2025-10-15 Illumina, Inc. Methods for addressing inefficiencies in amplification reactions
CN111183229A (zh) 2017-08-11 2020-05-19 阿提拉生物系统股份有限公司 使用有限核苷酸组成的引物的数字扩增
CN107868837B (zh) * 2017-12-12 2019-03-01 苏州普瑞森基因科技有限公司 一种用于分析肠道微生物的引物组合物及其应用
CN109988826A (zh) * 2017-12-30 2019-07-09 安诺优达基因科技(北京)有限公司 一种基于str计算混合样本嵌合比例的方法以及系统
EP3517629A1 (en) * 2018-01-30 2019-07-31 Myway Genetics S.r.L. Use of cfdna fragments as biomarkers in patients after organ transplantation
CN108342469A (zh) * 2018-02-27 2018-07-31 广州中安基因科技有限公司 一种皮肤基因检测的基因芯片
AU2019240244A1 (en) * 2018-03-22 2020-11-19 President And Fellows Of Harvard College Methods and compositions for molecular authentication
JP6646120B1 (ja) * 2018-10-01 2020-02-14 日本ソフトウェアマネジメント株式会社 分解されたdnaで個体識別可能なdna鑑定方法
KR102097721B1 (ko) * 2019-01-24 2020-04-06 주식회사 시선바이오머티리얼스 태그서열 snp를 이용한 단일 검출 프로브 기반 다중 표적 검출방법
CN111485024B (zh) * 2019-01-29 2024-04-26 深圳华大法医科技有限公司 用于个体特征确认的引物组合、及其应用
KR102351579B1 (ko) * 2019-06-25 2022-01-17 대한민국 상염색체 str 좌위를 포함한 ngs 패널 및 다중증폭 시스템
EP4028546A1 (en) * 2019-09-11 2022-07-20 Parlanca Limited A multiplex method of preparing a sequencing library
BR112022019785A2 (pt) * 2020-03-31 2022-11-16 Personal Genome Diagnostics Inc Composições e métodos para determinação da qualidade de ácido nucleico
CN112592981B (zh) * 2020-12-01 2023-06-20 广州精科医学检验所有限公司 用于dna档案建库的引物组、试剂盒和方法
CN112342303A (zh) * 2020-12-04 2021-02-09 郑州高新生物技术有限公司 一种基于ngs的人类y染色体str和snp遗传标记联合检测体系及检测方法
CN112852970A (zh) * 2020-12-31 2021-05-28 百特元生物科技(北京)有限公司 一种同时扩增人37个y-str基因座的引物组、试剂盒及其应用
JP2024507168A (ja) * 2021-02-12 2024-02-16 ベロジェン・インコーポレイテッド Dnaベースの血縁関係解析のための方法および組成物
CN113293205B (zh) * 2021-05-24 2024-11-26 深圳市真迈生物科技有限公司 测序方法
KR102337267B1 (ko) * 2021-06-01 2021-12-08 주식회사 다우진유전자연구소 유전자 감식을 위한 24개의 str 유전자 마커를 이용한 pcr 다중증폭 시스템
CN113930490B (zh) * 2021-09-27 2024-09-10 江汉大学 二代测序平台中利用分子特异性条形码评估str滑脱的方法及其应用
WO2023064818A1 (en) * 2021-10-13 2023-04-20 Verogen, Inc. Methods and compositions for improving accuracy of dna based kinship analysis
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
CN119731731A (zh) 2022-08-16 2025-03-28 诺杰有限公司 用于失踪人员和灾难/冲突受害者的亲属关系评估的方法和系统
EP4599059A2 (en) * 2022-10-21 2025-08-13 Watchmaker Genomics, Inc. Methods and compositions for sequencing library normalization
EP4603595A1 (en) * 2024-02-13 2025-08-20 ImmuneDiscover Sweden AB A method for typing the immune genes and the allelic variants thereof

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP1591541B1 (en) 1997-04-01 2012-02-15 Illumina Cambridge Limited Method of nucleic acid sequencing
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
GB9929381D0 (en) * 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
DE60131194T2 (de) 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire Sequenzbestimmung in echtzeit
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7078168B2 (en) * 2001-02-27 2006-07-18 Biotage Ab Method for determining allele frequencies
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Gatewayエントリークローンの作製方法
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
ES2949821T3 (es) 2004-01-07 2023-10-03 Illumina Cambridge Ltd Matrices moleculares
WO2006004659A1 (en) 2004-06-30 2006-01-12 Applera Corporation Methods for analyzing short tandem repeats and single nucleotide polymorphisms
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
EP3415641B1 (en) 2004-09-17 2023-11-01 Pacific Biosciences Of California, Inc. Method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
US8623628B2 (en) 2005-05-10 2014-01-07 Illumina, Inc. Polymerases
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CN101460953B (zh) 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
WO2007147018A1 (en) * 2006-06-14 2007-12-21 Cellpoint Diagnostics, Inc. Analysis of rare cell-enriched samples
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US9434997B2 (en) * 2007-08-24 2016-09-06 Lawrence Livermore National Security, Llc Methods, compounds and systems for detecting a microorganism in a sample
US20090163366A1 (en) * 2007-12-24 2009-06-25 Helicos Biosciences Corporation Two-primer sequencing for high-throughput expression analysis
EP2271772B1 (en) * 2008-03-11 2014-07-16 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US10662474B2 (en) 2010-01-19 2020-05-26 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic DNA by whole genome sequencing
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CA3106132C (en) 2011-05-12 2023-08-29 Ande Corporation Methods and compositions for rapid multiplex amplification of str loci
EP3290528B1 (en) 2011-09-23 2019-08-14 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2013151622A1 (en) 2012-04-03 2013-10-10 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
WO2014004852A2 (en) * 2012-06-29 2014-01-03 General Electric Company Kit for isothermal dna amplification starting from an rna template
CN106164298B (zh) * 2014-02-18 2020-02-21 伊鲁米那股份有限公司 用于dna谱系分析的方法及组合物

Also Published As

Publication number Publication date
AU2015219289A1 (en) 2016-09-08
MY200537A (en) 2024-01-02
WO2015126766A1 (en) 2015-08-27
RU2708337C2 (ru) 2019-12-05
US20200224268A1 (en) 2020-07-16
AU2021203877B2 (en) 2023-11-23
CA2940048A1 (en) 2016-08-27
HUE055256T2 (hu) 2021-11-29
HRP20210953T1 (hr) 2021-09-03
JP2021177777A (ja) 2021-11-18
US20150232929A1 (en) 2015-08-20
RU2019138698A3 (es) 2020-06-19
AU2015219289B2 (en) 2021-05-06
EP3108009A1 (en) 2016-12-28
US11530446B2 (en) 2022-12-20
RU2019138698A (ru) 2020-01-27
RU2016133987A (ru) 2018-03-27
BR112016019267B8 (pt) 2023-01-10
BR112016019267A2 (pt) 2017-10-10
MY182415A (en) 2021-01-25
NZ723399A (en) 2020-08-28
AU2021203877A1 (en) 2021-07-08
RS61976B1 (sr) 2021-07-30
JP2024059651A (ja) 2024-05-01
BR122022007092B8 (pt) 2023-01-31
NZ761724A (en) 2024-04-26
CN106164298A (zh) 2016-11-23
DK3108009T3 (da) 2021-06-07
PT3108009T (pt) 2021-05-10
JP2017509329A (ja) 2017-04-06
CA2940048C (en) 2023-03-14
RU2752700C2 (ru) 2021-07-30
PL3108009T3 (pl) 2021-10-11
MX379233B (es) 2025-03-10
BR122022007092B1 (pt) 2022-07-19
BR112016019267B1 (pt) 2022-06-28
US10422002B2 (en) 2019-09-24
EP3108009B1 (en) 2021-03-24
RU2016133987A3 (es) 2018-12-19
EP3910069A1 (en) 2021-11-17
WO2015126766A8 (en) 2016-11-03
CN106164298B (zh) 2020-02-21
LT3108009T (lt) 2021-07-12
ES2866044T3 (es) 2021-10-19
JP7770437B2 (ja) 2025-11-14
SI3108009T1 (sl) 2021-09-30
JP7011392B2 (ja) 2022-01-26
SA516371691B1 (ar) 2022-03-20

Similar Documents

Publication Publication Date Title
MX2016010717A (es) Métodos y composiciones para el perfilado de adn.
CY1125029T1 (el) Τροποποιημενοι νουκλεοζιτες, νουκλεοτιδια, και νουκλεϊκα οξεα και χρησεις αυτων
CY1120520T1 (el) Συναρμολογηση dna διαμεσολαβουμενη απο νουκλεαση
GB2537077A (en) Methods for sequencing nucleic acids
CO2017011957A2 (es) Variantes de alfa-amilasa y polinucleótidos que codifican las mismas
GB2544001A (en) Methods for increasing Cas9-mediated engineering efficiency
PL3216867T3 (pl) Sposób modyfikowania sekwencji genomu w celu wprowadzania swoistej mutacji do docelowej sekwencji DNA poprzez reakcję usuwania zasady i stosowany w nim kompleks cząsteczkowy
WO2015196128A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015196130A3 (en) Alternative nucleic acid molecules and uses thereof
EP3204397C0 (en) GALNAC PHOSPHORAMIDITES, THEIR CONJUGATE WITH NUCLEIC ACIDS AND THEIR USE
MX2016007541A (es) Moléculas modificadas de ácido nucleico y usos de las mismas.
MX2016012508A (es) Detección de secuencia por objetivo de ácido nucleico usando diferentes temperaturas de detección.
DK3348636T3 (da) Fremgangsmåde til modificering af en genomsekvens, der specifikt omdanner nukleobasen af en targetteret DNA-sekvens, og molekylær sekvens anvendt i fremgangsmåden
HUE052552T2 (hu) Javított nukleinsav-konstrukciók eukarióta génexpresszióhoz
BR112017011510A2 (pt) edição de rna direcionado
MX2017001405A (es) Deteccion de acidos nucleicos diana mediante el uso de hibridacion.
MX2019012252A (es) Composiciones y metodos para inhibir la expresion del gen alas1.
EA201692535A1 (ru) Замещенные нуклеозиды, нуклеотиды и их аналоги
HK1252166A1 (zh) 从环状rna产生环状dna的方法
CY1122975T1 (el) Συνθεσεις και μεθοδοι για την αναστολη της εκφρασης του γονιδιου alas1
GB201510723D0 (en) High throughput isothermal nucleic acid amplification
MX2018014163A (es) Composiciones de nucleotido marcados y metodos para el secuenciamiento de acido nucleico.
IL277813A (en) Tm-enhanced double-helix nucleic acid tracer molecules with nucleotides
MX2017014206A (es) Introgresion de un rendimieno qtl en plantas de cucumis sativus.
MX2017004034A (es) Mutantes de recombinasas.