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MX2016000128A - Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas. - Google Patents

Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.

Info

Publication number
MX2016000128A
MX2016000128A MX2016000128A MX2016000128A MX2016000128A MX 2016000128 A MX2016000128 A MX 2016000128A MX 2016000128 A MX2016000128 A MX 2016000128A MX 2016000128 A MX2016000128 A MX 2016000128A MX 2016000128 A MX2016000128 A MX 2016000128A
Authority
MX
Mexico
Prior art keywords
filamentous fungal
mutation
mannosyltransferase
fungal cell
methods
Prior art date
Application number
MX2016000128A
Other languages
English (en)
Other versions
MX364532B (es
Inventor
Markku Saloheimo
Jari Natunen
Jukka Hiltunen
Anne Huuskonen
Christian Ostermeier
Benjamin Patrick Sommer
Ramon Wahl
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48740957&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016000128(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of MX2016000128A publication Critical patent/MX2016000128A/es
Publication of MX364532B publication Critical patent/MX364532B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01109—Dolichyl-phosphate-mannose-protein mannosyltransferase (2.4.1.109)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)

Abstract

La presente descripción se refiere a composiciones y métodos útiles para la producción de proteínas heterólogas con O-manosilación reducida en células de hongos filamentosos, como células Trichoderma; más específicamente, la invención proporciona una célula de hongos filamentosos deficiente de PMT que comprende a) al menos una primera mutación que reduce una actividad de proteasa endógena en comparación con una célula de hongos filamentosos parentales que no tienen dicha primera mutación y, b) al menos una segunda mutación en un gen PMT que reduce la actividad endógena de 0-manosiltransferasa comparada con una célula de hongos filamentosos parentales que no tienen dicha segunda mutación, en donde dicha célula de hongos filamentosos es seleccionada del grupo consistente en células Trichoderma, Neurospora, Myceliophthora o Chrysosporium.
MX2016000128A 2013-07-04 2014-07-03 Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas. MX364532B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13175141 2013-07-04
PCT/EP2014/064248 WO2015001049A1 (en) 2013-07-04 2014-07-03 O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof

Publications (2)

Publication Number Publication Date
MX2016000128A true MX2016000128A (es) 2016-03-01
MX364532B MX364532B (es) 2019-04-29

Family

ID=48740957

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016000128A MX364532B (es) 2013-07-04 2014-07-03 Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.

Country Status (15)

Country Link
US (4) US20160376570A1 (es)
EP (1) EP3016970B1 (es)
KR (1) KR102111078B1 (es)
CN (1) CN105492457A (es)
BR (1) BR112015032695A2 (es)
CA (1) CA2916594C (es)
DK (1) DK3016970T3 (es)
ES (1) ES2739280T3 (es)
HR (1) HRP20191069T1 (es)
MX (1) MX364532B (es)
PL (1) PL3016970T3 (es)
RU (1) RU2016103373A (es)
SG (1) SG11201510814YA (es)
SI (1) SI3016970T1 (es)
WO (1) WO2015001049A1 (es)

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CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
US20210388310A1 (en) * 2018-09-20 2021-12-16 Perfect Day, Inc. Methods and compositions for producing homokaryotic filamentous fungal cells
WO2020112881A1 (en) * 2018-11-28 2020-06-04 Novozymes A/S Modified filamentous fungal host cells
CN110885810B (zh) * 2019-12-16 2021-03-23 华中农业大学 一种利用真核发酵制备得到的抑菌蛋白酶CmYC1及其应用
CN115976033B (zh) * 2022-12-14 2024-05-17 西南大学 瓜实蝇tssk1和tssk3基因及其应用
WO2025005761A1 (ko) * 2023-06-29 2025-01-02 씨제이제일제당 (주) 아스파르트산 프로테아제 변이체
CN119931997B (zh) * 2025-04-09 2025-07-25 安琪酵母股份有限公司 天冬氨酸蛋白酶突变体及其应用

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Also Published As

Publication number Publication date
US20220213451A1 (en) 2022-07-07
EP3016970A1 (en) 2016-05-11
KR102111078B1 (ko) 2020-05-18
US20160376570A1 (en) 2016-12-29
DK3016970T3 (da) 2019-06-24
CN105492457A (zh) 2016-04-13
US20200040314A1 (en) 2020-02-06
US20200362319A1 (en) 2020-11-19
PL3016970T3 (pl) 2019-09-30
BR112015032695A2 (pt) 2017-10-24
RU2016103373A (ru) 2017-08-10
KR20160030127A (ko) 2016-03-16
EP3016970B1 (en) 2019-05-22
US10724013B2 (en) 2020-07-28
SI3016970T1 (sl) 2019-08-30
WO2015001049A1 (en) 2015-01-08
MX364532B (es) 2019-04-29
SG11201510814YA (en) 2016-01-28
ES2739280T3 (es) 2020-01-30
CA2916594A1 (en) 2015-01-08
RU2016103373A3 (es) 2018-03-26
CA2916594C (en) 2020-03-10
HRP20191069T1 (hr) 2019-11-01

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