MX2012001000A - Metodo y kit para la deteccion de un microorganismo. - Google Patents
Metodo y kit para la deteccion de un microorganismo.Info
- Publication number
- MX2012001000A MX2012001000A MX2012001000A MX2012001000A MX2012001000A MX 2012001000 A MX2012001000 A MX 2012001000A MX 2012001000 A MX2012001000 A MX 2012001000A MX 2012001000 A MX2012001000 A MX 2012001000A MX 2012001000 A MX2012001000 A MX 2012001000A
- Authority
- MX
- Mexico
- Prior art keywords
- test sample
- nucleic acid
- cells
- microorganisms
- rna
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 239000000825 pharmaceutical preparation Substances 0.000 abstract 2
- 239000003431 cross linking reagent Substances 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Las células vivas de un microorganismo en una muestra de prueba se detectan distinguiendo las células vivas de las células muertas o las células dañadas mediante los siguientes pasos de: a) agregar un agente capaz de unirse de manera covalente al ADN o ARN mediante la irradiación con una luz, que tiene una longitud de onda de 350 nm a 700 nm a la muestra de prueba; b) irradiar la muestra de prueba a la cual se agrega un agente reticulante con una luz que tiene una longitud de onda of 350 nm a 700 nm; c) amplificar una región objetivo de ADN o ARN del microorganismo contenido en la muestra de prueba mediante un método de amplificación de los ácidos nucleicos en la presencia de un agente para suprimir la acción de una sustancia inhibidora de la amplificación de los ácidos nucleicos, sin extraer los ácidos nucleicos de las células; y d) analizar el producto amplificado.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009173566 | 2009-07-24 | ||
| PCT/JP2010/062474 WO2011010740A1 (ja) | 2009-07-24 | 2010-07-23 | 微生物検出法及び微生物検出キット |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2012001000A true MX2012001000A (es) | 2012-03-16 |
Family
ID=43499212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012001000A MX2012001000A (es) | 2009-07-24 | 2010-07-23 | Metodo y kit para la deteccion de un microorganismo. |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9394572B2 (es) |
| EP (1) | EP2458002B1 (es) |
| JP (1) | JP4825313B2 (es) |
| KR (1) | KR101383389B1 (es) |
| CN (2) | CN103820578B (es) |
| AU (1) | AU2010275576B2 (es) |
| CA (1) | CA2768699C (es) |
| MX (1) | MX2012001000A (es) |
| NZ (1) | NZ597138A (es) |
| RU (1) | RU2527897C2 (es) |
| SG (1) | SG177738A1 (es) |
| WO (1) | WO2011010740A1 (es) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103443294B (zh) * | 2011-01-24 | 2015-09-30 | 宝生物工程株式会社 | 用于修饰核酸的方法 |
| WO2013006793A2 (en) * | 2011-07-06 | 2013-01-10 | Quest Diagnostics Investments Incorporated | Direct amplification and detection of viral and bacterial pathogens |
| US9709500B2 (en) | 2012-05-02 | 2017-07-18 | Charles River Laboratories, Inc. | Optical method for detecting viable microorganisms in a cell sample |
| EP2769204B1 (en) | 2012-05-02 | 2016-02-17 | Charles River Laboratories, Inc. | Cell capture system and use thereof |
| US8993260B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher |
| KR20150127850A (ko) * | 2012-07-13 | 2015-11-18 | 솔젠트 (주) | 중합효소 연쇄반응을 이용한 분자진단검사용 중합효소 및 보조효소의 ｄｎａ 오염 제거를 위한 정제 방법 |
| DE102012014981B3 (de) | 2012-07-26 | 2013-07-18 | Sartorius Stedim Biotech Gmbh | Verfahren zur Differenzierung zwischen lebenden und toten Zellen |
| CN102851381A (zh) * | 2012-09-21 | 2013-01-02 | 武汉真福医药科技发展有限公司 | 快速检测单增李斯特菌的lamp试剂盒 |
| CN104278024B (zh) * | 2013-07-05 | 2016-12-28 | 中国人民解放军军事医学科学院微生物流行病研究所 | 用于鉴定人类腺病毒55型的引物组合物以及它们的应用 |
| JP6139425B2 (ja) * | 2014-01-30 | 2017-05-31 | 森永乳業株式会社 | 微生物検出法及び微生物検出キット |
| JP2015188343A (ja) * | 2014-03-27 | 2015-11-02 | セイコーエプソン株式会社 | バイオチップ |
| WO2017010001A1 (ja) * | 2015-07-16 | 2017-01-19 | 森永乳業株式会社 | 微生物検出法及び微生物検出キット |
| JP6979269B2 (ja) * | 2016-10-28 | 2021-12-08 | 森永乳業株式会社 | 微生物の死細胞及び／又は不活化ウイルスの測定方法 |
| CN107058517B (zh) * | 2017-03-13 | 2021-05-28 | 新乡医学院第一附属医院 | 一种用于结核分枝杆菌感染检测的试剂盒及检测方法 |
| CN106995805A (zh) * | 2017-03-26 | 2017-08-01 | 海南大学 | 一种溶菌酶标记的工程化噬菌体快速检测微生物 |
| CN107326094A (zh) * | 2017-09-04 | 2017-11-07 | 重庆市畜牧科学院 | 基于环介导等温扩增检测鸡鼻炎型克雷伯氏菌的试剂盒及其检测方法 |
| JP7350716B2 (ja) * | 2018-03-27 | 2023-09-26 | 森永乳業株式会社 | 微生物の細胞及び／又はウイルスの測定方法 |
| CN108588188B (zh) * | 2018-04-28 | 2021-11-09 | 天津科技大学 | 一种混合微生物发酵过程中微生物定量检测方法 |
| CN109295174B (zh) * | 2018-10-22 | 2022-05-27 | 益善生物技术股份有限公司 | 一种用于检测真菌感染的试剂组、试剂盒以及检测方法 |
| TR201820388A2 (tr) | 2018-12-25 | 2019-01-21 | Tuerkiye Bilimsel Ve Teknolojik Arastirma Kurumu Tuebitak | Salmonellanin klasi̇k kültür metoduna alternati̇f hizli ve taşinabi̇li̇r mi̇kroakişkan tespi̇t si̇stemi̇ |
| JP7535312B2 (ja) * | 2019-02-28 | 2024-08-16 | デイ ゼロ ダイアグノスティックス， インコーポレイテッド | 核酸増幅用の臨床サンプルを調製する改善された方法 |
| WO2020182924A1 (en) * | 2019-03-14 | 2020-09-17 | Merck Patent Gmbh | A novel sampling method for long-term monitoring of microbes |
| WO2020218553A1 (ja) * | 2019-04-26 | 2020-10-29 | ｂｉｔＢｉｏｍｅ株式会社 | デジタル微生物叢解析 |
| JPWO2020218557A1 (es) * | 2019-04-26 | 2020-10-29 | ||
| US11453907B2 (en) | 2020-03-23 | 2022-09-27 | The Broad Institute, Inc. | Crispr effector system based coronavirus diagnostics |
| CN112063732B (zh) * | 2020-09-17 | 2022-05-31 | 扬州大学 | 一种能够特异性识别阪崎肠杆菌存活细胞的快速定量检测方法及其引物 |
| JPWO2022071469A1 (es) * | 2020-09-30 | 2022-04-07 | ||
| CN113652358B (zh) * | 2021-09-15 | 2023-09-12 | 合肥工业大学 | 一种基于竹红菌素的光动力处理抑制克罗诺杆菌生长的用途 |
| CN115851441A (zh) * | 2022-11-15 | 2023-03-28 | 深圳科君生物科技有限公司 | 一种用于从生物样本中分离活菌的密度梯度离心法 |
| CN117487896B (zh) * | 2023-12-29 | 2024-06-25 | 清华大学 | 拮抗剂以及含有其的pcr预混液、pcr检测试剂盒和pcr检测方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001008685A (ja) * | 1999-06-25 | 2001-01-16 | Shimadzu Corp | 核酸合成法 |
| GB0008787D0 (en) | 2000-04-10 | 2000-05-31 | Norsk Naeringsmiddelforskning | A method of cell detection |
| WO2002052034A1 (fr) | 2000-12-26 | 2002-07-04 | Joji Oshima | Methodes de bioscopie et d'amplification d'acides nucleiques |
| WO2004104196A1 (ja) | 2003-05-20 | 2004-12-02 | G & G Science Co., Ltd. | 緩衝剤組成物 |
| CN101287843B (zh) | 2005-07-21 | 2012-11-07 | 森永乳业株式会社 | 用于检测微生物的方法及试剂盒 |
| KR20100115383A (ko) | 2006-02-17 | 2010-10-27 | 모리나가 뉴교 가부시키가이샤 | 미생물 검출법 및 미생물 검출 키트 |
| US8221975B2 (en) * | 2007-08-16 | 2012-07-17 | Morinaga Milk Industry Co., Ltd. | Method for detection of microorganism |
-
2010
- 2010-07-23 WO PCT/JP2010/062474 patent/WO2011010740A1/ja not_active Ceased
- 2010-07-23 SG SG2012005013A patent/SG177738A1/en unknown
- 2010-07-23 MX MX2012001000A patent/MX2012001000A/es not_active Application Discontinuation
- 2010-07-23 CA CA2768699A patent/CA2768699C/en active Active
- 2010-07-23 EP EP10802360.7A patent/EP2458002B1/en active Active
- 2010-07-23 US US13/386,601 patent/US9394572B2/en active Active
- 2010-07-23 CN CN201410067344.4A patent/CN103820578B/zh not_active Expired - Fee Related
- 2010-07-23 CN CN201080033156.XA patent/CN102471768B/zh not_active Expired - Fee Related
- 2010-07-23 NZ NZ597138A patent/NZ597138A/xx not_active IP Right Cessation
- 2010-07-23 AU AU2010275576A patent/AU2010275576B2/en not_active Ceased
- 2010-07-23 RU RU2012106617/10A patent/RU2527897C2/ru active
- 2010-07-23 KR KR1020127004714A patent/KR101383389B1/ko not_active Expired - Fee Related
- 2010-07-23 JP JP2010529962A patent/JP4825313B2/ja active Active
-
2016
- 2016-06-21 US US15/188,755 patent/US10329604B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20120048647A (ko) | 2012-05-15 |
| EP2458002B1 (en) | 2015-03-25 |
| US10329604B2 (en) | 2019-06-25 |
| CA2768699C (en) | 2017-08-22 |
| US20120122082A1 (en) | 2012-05-17 |
| NZ597138A (en) | 2012-12-21 |
| CN102471768B (zh) | 2014-07-23 |
| US20160298181A1 (en) | 2016-10-13 |
| EP2458002A4 (en) | 2013-01-30 |
| CN102471768A (zh) | 2012-05-23 |
| US9394572B2 (en) | 2016-07-19 |
| RU2527897C2 (ru) | 2014-09-10 |
| EP2458002A1 (en) | 2012-05-30 |
| CN103820578A (zh) | 2014-05-28 |
| JP4825313B2 (ja) | 2011-11-30 |
| WO2011010740A1 (ja) | 2011-01-27 |
| SG177738A1 (en) | 2012-02-28 |
| KR101383389B1 (ko) | 2014-04-08 |
| CA2768699A1 (en) | 2011-01-27 |
| AU2010275576A1 (en) | 2012-01-19 |
| JPWO2011010740A1 (ja) | 2013-01-07 |
| AU2010275576B2 (en) | 2013-02-28 |
| RU2012106617A (ru) | 2013-08-27 |
| CN103820578B (zh) | 2015-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |