MX2012002411A - Metodos de ensayo para el mdv-1. - Google Patents
Metodos de ensayo para el mdv-1.Info
- Publication number
- MX2012002411A MX2012002411A MX2012002411A MX2012002411A MX2012002411A MX 2012002411 A MX2012002411 A MX 2012002411A MX 2012002411 A MX2012002411 A MX 2012002411A MX 2012002411 A MX2012002411 A MX 2012002411A MX 2012002411 A MX2012002411 A MX 2012002411A
- Authority
- MX
- Mexico
- Prior art keywords
- mdv
- nucleic acid
- vaccine
- virulent
- snp
- Prior art date
Links
- 238000003556 assay Methods 0.000 title 1
- VHTUHGNVVZPWGO-UHFFFAOYSA-N 7-(2-hydroxyethyl)-1,3-dimethyl-8-(pyridin-3-ylmethyl)purine-2,6-dione Chemical compound OCCN1C=2C(=O)N(C)C(=O)N(C)C=2N=C1CC1=CC=CN=C1 VHTUHGNVVZPWGO-UHFFFAOYSA-N 0.000 abstract 4
- 239000000523 sample Substances 0.000 abstract 4
- 229960005486 vaccine Drugs 0.000 abstract 4
- 239000012472 biological sample Substances 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 241000700605 Viruses Species 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000011002 quantification Methods 0.000 abstract 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 238000011529 RT qPCR Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000003753 real-time PCR Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Un método para la cuantificación de una cepa de vacuna y/o una cepa virulenta del Serotipo-1 de Virus de Enfermedad de Marek (MDV-1) en una muestra de un ave que comprende las etapas de: (i) proporcionar una muestra biológica del ave y opcionalmente aislar el ácido nucleico de la muestra biológica; (ii) someter la muestra biológica de (i) a PCR cuantitativo de tiempo real (qPCR) que comprende: (a) amplificación de la región del gen pp38 dentro de la muestra de ácido nucleico de (i), tal región contiene una diferencia de polimorfismo de nucleótido simple (SNP) consistente entre las cepas de vacuna y virulenta del MDV-1; y (b) poner en contacto el ácido nucleico amplificado de (a) con una sonda de ácido nucleico detectable específica para el SNP de la cepa de vacuna del MDV-1 y/o una sonda de ácido nucleico detectable específica para el SNP de la cepa virulenta del MDV-1; e (iii) medir los cambios en la señal detectable producida por la sonda de (ii). Los métodos también se proporcionan para la cuantificación absoluta de los virus de vacuna y virulencia.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0914826.3A GB0914826D0 (en) | 2009-08-25 | 2009-08-25 | Assay methods |
| PCT/GB2010/001602 WO2011023950A1 (en) | 2009-08-25 | 2010-08-25 | Assay methods for mdv-1 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2012002411A true MX2012002411A (es) | 2012-04-10 |
Family
ID=41171882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012002411A MX2012002411A (es) | 2009-08-25 | 2010-08-25 | Metodos de ensayo para el mdv-1. |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US8865409B2 (es) |
| EP (1) | EP2470676B1 (es) |
| JP (2) | JP6025562B2 (es) |
| CN (1) | CN102686748B (es) |
| AU (1) | AU2010288269C1 (es) |
| BR (1) | BR112012004102B1 (es) |
| CA (1) | CA2771962C (es) |
| CY (1) | CY1116130T1 (es) |
| DK (1) | DK2470676T3 (es) |
| ES (1) | ES2538189T3 (es) |
| GB (1) | GB0914826D0 (es) |
| HR (1) | HRP20150308T1 (es) |
| MX (1) | MX2012002411A (es) |
| PL (1) | PL2470676T3 (es) |
| PT (1) | PT2470676E (es) |
| SI (1) | SI2470676T1 (es) |
| WO (1) | WO2011023950A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0914826D0 (en) | 2009-08-25 | 2009-09-30 | Wyeth Corp | Assay methods |
| US9631243B2 (en) * | 2012-06-18 | 2017-04-25 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods and compositions for identifying JC virus |
| EP2845904A1 (en) * | 2013-09-06 | 2015-03-11 | Ceva Sante Animale | Recombinant Marek's disease viruses and uses thereof |
| EP2889381A1 (en) * | 2013-12-27 | 2015-07-01 | Universite De Liege | Detection method for species specific identification of nucleic acid of interest |
| CN105886662B (zh) * | 2016-01-26 | 2020-03-24 | 金宇保灵生物药品有限公司 | 快速选育猪瘟兔化弱毒株(c株)st敏感细胞系的方法及专用引物 |
| CN108034767A (zh) * | 2018-01-03 | 2018-05-15 | 山东农业大学 | 鉴别血清i型马立克病毒基因缺失疫苗、传代致弱疫苗与野毒的引物、探针及试剂盒 |
| WO2020098808A1 (en) | 2018-11-17 | 2020-05-22 | Beijing Bytedance Network Technology Co., Ltd. | Construction of merge with motion vector difference candidates |
| CN109355434B (zh) * | 2018-11-26 | 2022-04-15 | 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） | 定量检测鸡马立克氏病毒Meq基因缺失疫苗株和野毒株的荧光定量PCR试剂盒及其应用 |
| CN110699485B (zh) * | 2019-08-16 | 2023-03-28 | 广州千寻生物技术有限公司 | 一种快速检测马立克氏病病毒的rpa引物对、探针、试剂盒及检测方法 |
| CN111733297A (zh) * | 2020-08-10 | 2020-10-02 | 中国兽医药品监察所 | 一种马立克氏病病毒血清3型荧光定量pcr试剂盒及其应用 |
| CN113025734A (zh) * | 2021-03-19 | 2021-06-25 | 广东省农业科学院动物卫生研究所 | 鉴别布鲁氏菌疫苗株a19与野毒株的引物、探针及应用 |
| CN114317827B (zh) * | 2022-01-07 | 2023-03-24 | 河南省农业科学院 | 一种鉴别不同血清型马立克病病毒感染与免疫的多重pcr引物、方法及应用 |
| CN114540312B (zh) * | 2022-03-16 | 2023-03-24 | 河南省农业科学院 | 一种血清1型马立克病病毒pp38单克隆抗体的制备方法及应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5827738A (en) * | 1995-10-27 | 1998-10-27 | Board Of Trustees Operating Michigan State University | Sustainable chick cell line infected with Marek's disease virus |
| US5702659A (en) | 1995-11-30 | 1997-12-30 | Corning Incorporated | Honeycomb extrusion die and methods |
| GB0914826D0 (en) | 2009-08-25 | 2009-09-30 | Wyeth Corp | Assay methods |
| CN101824488A (zh) * | 2010-04-09 | 2010-09-08 | 山东农业大学 | 鸡多重病毒核酸探针斑点杂交检测试剂盒 |
-
2009
- 2009-08-25 GB GBGB0914826.3A patent/GB0914826D0/en not_active Ceased
-
2010
- 2010-08-25 AU AU2010288269A patent/AU2010288269C1/en active Active
- 2010-08-25 US US13/391,563 patent/US8865409B2/en active Active
- 2010-08-25 DK DK10752901.8T patent/DK2470676T3/en active
- 2010-08-25 EP EP10752901.8A patent/EP2470676B1/en active Active
- 2010-08-25 ES ES10752901.8T patent/ES2538189T3/es active Active
- 2010-08-25 PL PL10752901T patent/PL2470676T3/pl unknown
- 2010-08-25 CN CN201080043079.6A patent/CN102686748B/zh active Active
- 2010-08-25 SI SI201030866T patent/SI2470676T1/sl unknown
- 2010-08-25 CA CA2771962A patent/CA2771962C/en active Active
- 2010-08-25 HR HRP20150308TT patent/HRP20150308T1/hr unknown
- 2010-08-25 WO PCT/GB2010/001602 patent/WO2011023950A1/en not_active Ceased
- 2010-08-25 MX MX2012002411A patent/MX2012002411A/es active IP Right Grant
- 2010-08-25 PT PT10752901T patent/PT2470676E/pt unknown
- 2010-08-25 JP JP2012526114A patent/JP6025562B2/ja active Active
- 2010-08-25 BR BR112012004102-9A patent/BR112012004102B1/pt active IP Right Grant
-
2014
- 2014-08-25 US US14/467,941 patent/US9416428B2/en active Active
- 2014-08-25 US US14/467,856 patent/US9416427B2/en active Active
-
2015
- 2015-03-20 CY CY20151100277T patent/CY1116130T1/el unknown
- 2015-09-28 JP JP2015189321A patent/JP2016052307A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2010288269B2 (en) | 2015-05-21 |
| US20150024382A1 (en) | 2015-01-22 |
| CA2771962A1 (en) | 2011-03-03 |
| EP2470676B1 (en) | 2015-01-28 |
| AU2010288269C1 (en) | 2015-11-26 |
| EP2470676A1 (en) | 2012-07-04 |
| DK2470676T3 (en) | 2015-02-16 |
| WO2011023950A1 (en) | 2011-03-03 |
| HRP20150308T1 (hr) | 2015-04-24 |
| CA2771962C (en) | 2015-09-29 |
| ES2538189T3 (es) | 2015-06-17 |
| CY1116130T1 (el) | 2017-02-08 |
| JP6025562B2 (ja) | 2016-11-16 |
| CN102686748B (zh) | 2015-09-09 |
| CN102686748A (zh) | 2012-09-19 |
| JP2013502916A (ja) | 2013-01-31 |
| JP2016052307A (ja) | 2016-04-14 |
| SI2470676T1 (sl) | 2015-03-31 |
| PT2470676E (pt) | 2015-03-30 |
| US9416428B2 (en) | 2016-08-16 |
| BR112012004102A2 (pt) | 2016-03-15 |
| PL2470676T3 (pl) | 2015-06-30 |
| US20120208177A1 (en) | 2012-08-16 |
| US20150024383A1 (en) | 2015-01-22 |
| GB0914826D0 (en) | 2009-09-30 |
| US8865409B2 (en) | 2014-10-21 |
| US9416427B2 (en) | 2016-08-16 |
| BR112012004102B1 (pt) | 2021-03-30 |
| AU2010288269A1 (en) | 2012-03-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GB | Transfer or rights |
Owner name: ZOETIS W LLC |
|
| HC | Change of company name or juridical status |
Owner name: ZOETIS W LLC |
|
| FG | Grant or registration |