[go: up one dir, main page]

WO2008111485A1 - モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット - Google Patents

モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット Download PDF

Info

Publication number
WO2008111485A1
WO2008111485A1 PCT/JP2008/054054 JP2008054054W WO2008111485A1 WO 2008111485 A1 WO2008111485 A1 WO 2008111485A1 JP 2008054054 W JP2008054054 W JP 2008054054W WO 2008111485 A1 WO2008111485 A1 WO 2008111485A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
compound
skeleton
mononucleoside
mononucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/054054
Other languages
English (en)
French (fr)
Inventor
Akimitsu Okamoto
Shuji Ikeda
Takeshi Kubota
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN
Original Assignee
RIKEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN filed Critical RIKEN
Priority to US12/530,574 priority Critical patent/US8383792B2/en
Priority to JP2009504009A priority patent/JP4761086B2/ja
Priority to EP08721474A priority patent/EP2130835B1/en
Priority to CN2008800076357A priority patent/CN101631796B/zh
Priority to ES08721474T priority patent/ES2385268T3/es
Publication of WO2008111485A1 publication Critical patent/WO2008111485A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

　例えば、核酸の二重らせん構造を効果的に検出可能な標識物質を提供する。 　モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物であって、前記構造が下記式（１）、（１ｂ）または（１ｃ）で表される化合物、その互変異性体若しくは立体異性体、またはそれらの塩を提供する。 　例えば、Ｂは、核酸塩基骨格を有する原子団であり、Ｅは、デオキシリボース骨格、リボース骨格、もしくはそれらのいずれかから誘導される構造を有する原子団、またはペプチド構造もしくはペプトイド構造を有する原子団であり、Ｚ１１およびＺ１２は、それぞれ、水素原子、保護基、または蛍光性を示す原子団であり、同一でも異なっていてもよい。  
PCT/JP2008/054054 2007-03-09 2008-03-06 モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット Ceased WO2008111485A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/530,574 US8383792B2 (en) 2007-03-09 2008-03-06 Compound having structure derived from mononucleoside or mononucleotide, nucleic acid, labeling substance, and method and kit for detection of nucleic acid
JP2009504009A JP4761086B2 (ja) 2007-03-09 2008-03-06 核酸、標識物質、核酸検出方法およびキット
EP08721474A EP2130835B1 (en) 2007-03-09 2008-03-06 Compound having structure derived from mononucleoside or mononucleotide, nucleic acid, labeling substance, and method and kit for detection of nucleic acid
CN2008800076357A CN101631796B (zh) 2007-03-09 2008-03-06 具有由单核苷或单核苷酸衍生的结构的化合物、核酸、标记物以及核酸检测方法和试剂盒
ES08721474T ES2385268T3 (es) 2007-03-09 2008-03-06 Compuesto que tiene una estructura derivada de mononucleósido o mononucleótido, ácido nucleico, sustancia marcadora y método y kit para la detección de ácido nucleico

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP2007-059921 2007-03-09
JP2007059921 2007-03-09
JP2007-246253 2007-09-21
JP2007246253 2007-09-21
JP2007-335352 2007-12-26
JP2007335352 2007-12-26

Publications (1)

Publication Number Publication Date
WO2008111485A1 true WO2008111485A1 (ja) 2008-09-18

Family

ID=39759427

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/054054 Ceased WO2008111485A1 (ja) 2007-03-09 2008-03-06 モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット

Country Status (6)

Country Link
US (1) US8383792B2 (ja)
EP (1) EP2130835B1 (ja)
JP (2) JP4761086B2 (ja)
KR (1) KR101551985B1 (ja)
ES (1) ES2385268T3 (ja)
WO (1) WO2008111485A1 (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010061922A1 (ja) 2008-11-27 2010-06-03 独立行政法人理化学研究所 新規ＭｕｔＳタンパク質およびそれを用いた変異の判定方法
WO2012091091A1 (ja) 2010-12-28 2012-07-05 独立行政法人理化学研究所 化合物、核酸、核酸の製造方法および核酸を製造するためのキット
WO2014034818A1 (ja) * 2012-08-30 2014-03-06 株式会社ダナフォーム 標的核酸の分析方法、キットおよび分析機器
WO2014038561A1 (ja) * 2012-09-04 2014-03-13 株式会社ダナフォーム 化合物、核酸、標識物質および検出方法
WO2015152024A1 (ja) * 2014-03-31 2015-10-08 独立行政法人理化学研究所 蛍光性標識一本鎖核酸及びその用途
JPWO2014013954A1 (ja) * 2012-07-16 2016-06-30 株式会社ダナフォーム 核酸プローブ、核酸プローブの設計方法、およびターゲット配列の検出方法

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008111485A1 (ja) * 2007-03-09 2008-09-18 Riken モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット
CA2622649C (en) * 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
RU2452944C1 (ru) * 2010-10-05 2012-06-10 Государственное образовательное учреждение высшего профессионального образования "Самарский государственный университет" Способ оценки подлинности лекарственного растительного сырья и устройство для его осуществления
EP2574669B1 (en) 2011-06-29 2018-10-17 Kabushiki Kaisha Dnaform Biological sample pretreatment method, method for detecting rna, and pretreatment kit
WO2013045487A1 (de) * 2011-09-28 2013-04-04 F. Hoffmann-La Roche Ag Azomediatoren
DK3041854T3 (da) 2013-08-08 2020-03-02 Scripps Research Inst En fremgangsmåde til stedspecifik enzymatisk mærkning af nukleinsyrer in vitro gennem inkorporering af unaturlige nukleotider
GB201410693D0 (en) 2014-06-16 2014-07-30 Univ Southampton Splicing modulation
WO2016054615A2 (en) 2014-10-03 2016-04-07 Cold Spring Harbor Laboratory Targeted augmentation of nuclear gene output
RU2741115C2 (ru) 2015-08-28 2021-01-22 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Способ анализа матричной нуклеиновой кислоты, способ анализа вещества-мишени, набор для анализа матричной нуклеиновой кислоты или вещества-мишени и анализатор для матричной нуклеиновой кислоты или вещества-мишени
IL308174A (en) 2015-10-09 2024-01-01 Univ Southampton Modulation of gene expression and screening for deregulated protein expression
KR102604132B1 (ko) 2015-12-14 2023-11-17 콜드스프링하버러보러토리 상염색체 우성 정신 지체 5 및 드라베 증후군의 치료를 위한 안티센스 올리고머
US11096956B2 (en) 2015-12-14 2021-08-24 Stoke Therapeutics, Inc. Antisense oligomers and uses thereof
WO2018025705A1 (ja) 2016-08-03 2018-02-08 国立研究開発法人理化学研究所 分析セル、分析デバイス、分析装置および分析システム
JP6448695B2 (ja) 2017-03-21 2019-01-09 株式会社東芝 Ｒｎａ増幅方法、ｒｎａ検出方法及びアッセイキット
CN111051512A (zh) 2017-07-11 2020-04-21 辛索克斯公司 非天然核苷酸的掺入及其方法
JP7055691B2 (ja) 2017-07-11 2022-04-18 株式会社東芝 短鎖核酸伸長用プライマーセット、アッセイキット、短鎖核酸伸長方法、増幅方法及び検出方法
EP3661956A4 (en) 2017-08-03 2021-04-28 Synthorx, Inc. CYTOKINE CONJUGATES FOR THE TREATMENT OF PROLIFERATIVES AND INFECTIOUS DISEASES
IL321831A (en) 2017-08-25 2025-08-01 Stoke Therapeutics Inc Antisense oligomers for the treatment of conditions and diseases
BR112020022512A2 (pt) 2018-05-04 2021-05-04 Stoke Therapeutics, Inc. métodos e composições para tratamento de doença de armazenamento de éster de colesteril
KR102627534B1 (ko) * 2018-06-21 2024-01-19 에프. 호프만-라 로슈 아게 전부-lna 올리고뉴클레오티드 혼성화
CN114949240B (zh) 2019-02-06 2025-09-23 新索思股份有限公司 Il-2缀合物及其使用方法
BR112022022889A2 (pt) 2020-05-11 2023-04-04 Stoke Therapeutics Inc Oligômeros antissentido de opa1 para tratamento de condições e doenças

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11127862A (ja) * 1997-10-31 1999-05-18 Canon Inc 標的核酸の検出方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69216498T2 (de) * 1991-08-13 1997-04-30 Bayer Ag Fluoreszierendes Etikett
DE4302459A1 (de) * 1993-01-29 1994-08-04 Bayer Ag Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren
US5986086A (en) * 1997-06-20 1999-11-16 Amersham Pharmacia Biotech Inc. Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides
US6743588B2 (en) * 2001-01-11 2004-06-01 Tosoh Corporation Fluorescent dye and method of measuring nucleic acid
JP4265134B2 (ja) 2001-01-11 2009-05-20 東ソー株式会社 新規蛍光色素及び核酸の測定方法
US20030165865A1 (en) 2001-01-29 2003-09-04 Hinkel Christopher A. Methods of analysis of nucleic acids
US20030082584A1 (en) 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
JP2003344290A (ja) 2002-05-27 2003-12-03 Aisin Cosmos R & D Co Ltd 温度調節付蛍光検出装置
JP2004081057A (ja) 2002-08-26 2004-03-18 Kansai Tlo Kk 蛍光ｒｎａプローブ
EP1594987B1 (en) * 2003-02-21 2009-05-27 GeneForm Technologies Limited Nucleic acid sequencing methods, kits and reagents
JP3897805B2 (ja) 2003-12-25 2007-03-28 独立行政法人理化学研究所 核酸の増幅法およびこれを利用した変異核酸の検出法
WO2006097320A2 (de) * 2005-03-17 2006-09-21 Genovoxx Gmbh Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
JP4920201B2 (ja) 2005-05-19 2012-04-18 日鉄環境エンジニアリング株式会社 核酸中の特定塩基を測定する方法
US20070048773A1 (en) * 2005-07-29 2007-03-01 Applera Corporation Detection of polyphosphate using fluorescently labeled polyphosphate acceptor substrates
WO2008111485A1 (ja) * 2007-03-09 2008-09-18 Riken モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット
CA2622649C (en) * 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11127862A (ja) * 1997-10-31 1999-05-18 Canon Inc 標的核酸の検出方法

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
ANALYTICA CHIMICA ACTA, vol. 470, 2002, pages 57 - 70
ANALYTICAL BIOCHEMISTRY, vol. 281, 2000, pages 26 - 35
BIOPOLYMERS, vol. 46, 1998, pages 39 - 51
CHEMISTRY - A EUROPEAN JOURNAL, vol. 12, 2006, pages 2270 - 2281
GELMINI, S. ET AL., CLIN. CHEM., vol. 43, 1997, pages 752 - 758
H. S. RYE ET AL.: "High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange", NUCLEIC ACIDS RES., vol. 19, 1991, pages 327 - 33
HRDLICKA, P. J. ET AL., J. AM. CHEM. SOC., vol. 127, 2005, pages 13293 - 13299
IKEDA S. ET AL.: "Kakusan Kenshutsu no Tameno Shinki Keiko Probe", SYMPOSIUM ON PHOTOCHEMISTRY, 21 September 2007 (2007-09-21), pages 182, XP008178464 *
L. G. LEE; C. H. CHEN; L. A. CHIU: "Thiazole orange: a new dye for reticulocyte analysis", CYTOMETRY, vol. 7, 1986, pages 508 - 17, XP000613662, DOI: doi:10.1002/cyto.990070603
NAZARENKO, I. A.; BHATNAGAR, S. K.; HOHMAN, R. J., NUCLEIC ACIDS RES., vol. 25, 1997, pages 2516 - 2521
ORGANIC LETTERS, vol. 6, 2000, pages 517 - 519
See also references of EP2130835A4
SVANVIK, N. ET AL., ANAL BIOCHEM., vol. 281, 2000, pages 26 - 35
TETRAHEDRON, vol. 43, no. 20, 1987, pages 4601 - 4607
TYAGI, S.; KRAMER, F. R., NAT. BIOTECHNOL., vol. 14, 1996, pages 303 - 308
WHITCOMBE, D. ET AL., NAT. BIOTECHNOL., vol. 17, 1999, pages 804 - 807

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010061922A1 (ja) 2008-11-27 2010-06-03 独立行政法人理化学研究所 新規ＭｕｔＳタンパク質およびそれを用いた変異の判定方法
JP5975524B2 (ja) * 2010-12-28 2016-08-23 国立研究開発法人理化学研究所 化合物、核酸、核酸の製造方法および核酸を製造するためのキット
WO2012091091A1 (ja) 2010-12-28 2012-07-05 独立行政法人理化学研究所 化合物、核酸、核酸の製造方法および核酸を製造するためのキット
US9499576B2 (en) 2010-12-28 2016-11-22 Riken Compound, nucleic, acid, method for producing nucleic acid, and kit for producing nucleic acid
US9862989B2 (en) 2012-07-16 2018-01-09 Kabushiki Kaisha Dnaform Nucleic acid probe, method for designing nucleic acid probe, and method for detecting target sequence
JPWO2014013954A1 (ja) * 2012-07-16 2016-06-30 株式会社ダナフォーム 核酸プローブ、核酸プローブの設計方法、およびターゲット配列の検出方法
US10066264B2 (en) 2012-08-30 2018-09-04 Kabushiki Kaisha Dnaform Method for analyzing target nucleic acid, kit, and analyzer
JPWO2014034818A1 (ja) * 2012-08-30 2016-08-08 株式会社ダナフォーム 標的核酸の分析方法、キットおよび分析機器
WO2014034818A1 (ja) * 2012-08-30 2014-03-06 株式会社ダナフォーム 標的核酸の分析方法、キットおよび分析機器
EA032482B1 (ru) * 2012-08-30 2019-06-28 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Способ анализа нуклеиновой кислоты-мишени
JPWO2014038561A1 (ja) * 2012-09-04 2016-08-12 株式会社ダナフォーム 化合物、核酸、標識物質および検出方法
WO2014038561A1 (ja) * 2012-09-04 2014-03-13 株式会社ダナフォーム 化合物、核酸、標識物質および検出方法
US10294261B2 (en) 2012-09-04 2019-05-21 Kabushiki Kaisha Dnaform Compound, nucleic acid, labeling substance, and detection method
EA034342B1 (ru) * 2012-09-04 2020-01-29 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Соединение, нуклеиновая кислота, вещество, несущее метку, и способ обнаружения
WO2015152024A1 (ja) * 2014-03-31 2015-10-08 独立行政法人理化学研究所 蛍光性標識一本鎖核酸及びその用途
JPWO2015152024A1 (ja) * 2014-03-31 2017-04-13 国立研究開発法人理化学研究所 蛍光性標識一本鎖核酸及びその用途
US10428371B2 (en) 2014-03-31 2019-10-01 Kabushiki Kaisha Dnaform Fluorescent labeled single-stranded nucleic acid and use thereof
EA037029B1 (ru) * 2014-03-31 2021-01-28 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Флуоресцентно меченная одноцепочечная нуклеиновая кислота и ее применение

Also Published As

Publication number Publication date
JP4761086B2 (ja) 2011-08-31
KR101551985B1 (ko) 2015-09-09
EP2130835A4 (en) 2010-06-16
ES2385268T3 (es) 2012-07-20
KR20090117814A (ko) 2009-11-12
US20100092971A1 (en) 2010-04-15
JPWO2008111485A1 (ja) 2010-06-24
EP2130835B1 (en) 2012-05-23
EP2130835A1 (en) 2009-12-09
US8383792B2 (en) 2013-02-26
JP2011173891A (ja) 2011-09-08

Similar Documents

Publication Publication Date Title
WO2008111485A1 (ja) モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット
IL198741A0 (en) Hepatitis c virus inhibitor compounds, compositions containing the same and uses thereof
WO2008060927A3 (en) Hepatitis c virus inhibitors
EP1970453A3 (en) Primer, primer set, and nucleic acid amplification method and mutation detection method using the same
WO2011139699A3 (en) 5' modified nucleosides and oligomeric compounds prepared therefrom
WO2008036168A3 (en) New small molecule inhibitors of mdm2 and the uses thereof
WO2006091646A3 (en) Small molecule inhibitors of mdm2 and uses thereof
UA91255C2 (uk) Антивірусні нуклеозиди
EP3330277A3 (en) Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
WO2008156139A1 (ja) 同位体化合物を標識として使用するタンパク質の分析方法
NO20075616L (no) Tripeptider som hepatitt C virus inhibitorer
MD4403B1 (ro) Compuşi antivirali în bază de dihidroxiizocromen-naftoimidazoli condensaţi
WO2009143369A3 (en) Method of preparing nucleosides and analogs thereof without using chromatography
EA201101240A1 (ru) Ингибиторы вируса гепатита с
MX2010002904A (es) N-fenil-dioxo-hidropirimidinas utiles como inhibidores de virus de hepatitis c (hcv).
MX2010004566A (es) Prueba de deteccion de bordetella.
WO2009051387A3 (en) Process for preparation of compound containing 6a group element using reductant
MY160455A (en) Detection and enumeration of microorganisms
IL189883A0 (en) Isoquinolines as igf-1r inhibitors
NO20081206L (no) Isoquinolines derivatives as IGF -1R inhibitors
WO2008088051A1 (ja) ホスホリルコリン基含有化合物、ホスホリルコリン基含有化合物の製造方法、表面改質剤、及び表面改質剤を用いた表面改質方法
NZ592385A (en) Highly purified polylactic acid or a derivative thereof, a salt of the same, and purification method thereof
UA101018C2 (ru) Пирролопиримидинкарбоксамиды
WO2007053193A3 (en) Unsolvated benzodiazepine compositions and methods
WO2010149598A3 (en) Heterocyclic antiviral compound

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200880007635.7

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08721474

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2009504009

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 12530574

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008721474

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 1020097019542

Country of ref document: KR