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WO2008090760A1 - 蛍光検出装置、マイクロチップ、及び検査システム - Google Patents

蛍光検出装置、マイクロチップ、及び検査システム Download PDF

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Publication number
WO2008090760A1
WO2008090760A1 PCT/JP2008/050117 JP2008050117W WO2008090760A1 WO 2008090760 A1 WO2008090760 A1 WO 2008090760A1 JP 2008050117 W JP2008050117 W JP 2008050117W WO 2008090760 A1 WO2008090760 A1 WO 2008090760A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescent
detector
microchip
nucleic acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/050117
Other languages
English (en)
French (fr)
Inventor
Tsuneo Sawazumi
Akihisa Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Konica Minolta Medical and Graphic Inc
Original Assignee
Konica Minolta Medical and Graphic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Konica Minolta Medical and Graphic Inc filed Critical Konica Minolta Medical and Graphic Inc
Priority to JP2008555011A priority Critical patent/JPWO2008090760A1/ja
Publication of WO2008090760A1 publication Critical patent/WO2008090760A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　標的ＤＮＡと蛍光標識したプローブとのハイブリダイゼーションにより蛍光共鳴エネルギー遷移現象に変化を生ずる反応を用いて標的核酸配列を蛍光検出を利用して検出する手法、すなわちサイクリング・プローブ法に代表される手法によって、簡易な構成でありながら、標識となる蛍光強度の測定を高感度・高精度に実現することができる蛍光検出装置、マイクロチップ、及びそれらから構成される蛍光検出装置を提供することを目的とする。 そのためには、標的ＤＮＡと蛍光標識したプローブとのハイブリダイゼーションにより蛍光共鳴エネルギー遷移現象に変化を生ずる反応を用いて標的核酸配列を検出する蛍光検出装置であって、蛍光発光をさせるための励起光の光源部と励起により発生する蛍光の検出部とを有し、該励起光の発光スペクトルの極大発光波長より長波長側部分が該蛍光の発光スペクトルと少なくとも部分的に重なることを特徴とする蛍光検出装置とする。
PCT/JP2008/050117 2007-01-26 2008-01-09 蛍光検出装置、マイクロチップ、及び検査システム Ceased WO2008090760A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008555011A JPWO2008090760A1 (ja) 2007-01-26 2008-01-09 蛍光検出装置、マイクロチップ、及び検査システム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-016155 2007-01-26
JP2007016155 2007-01-26

Publications (1)

Publication Number Publication Date
WO2008090760A1 true WO2008090760A1 (ja) 2008-07-31

Family

ID=39644335

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050117 Ceased WO2008090760A1 (ja) 2007-01-26 2008-01-09 蛍光検出装置、マイクロチップ、及び検査システム

Country Status (2)

Country Link
JP (1) JPWO2008090760A1 (ja)
WO (1) WO2008090760A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103782157A (zh) * 2011-08-30 2014-05-07 奥林巴斯株式会社 包含胰液的生物试样中的目标粒子的检测方法
CN104076004A (zh) * 2014-07-16 2014-10-01 常熟理工学院 一种检测样品中汞离子浓度的方法
CN104280373A (zh) * 2014-09-23 2015-01-14 天津科技大学 利用荧光增强法检测二、三价铁离子的方法
WO2022136402A1 (en) * 2020-12-22 2022-06-30 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001255328A (ja) * 2000-03-10 2001-09-21 Hitachi Software Eng Co Ltd ハイブリダイゼーション反応検出方法及び検出装置
JP2004233114A (ja) * 2003-01-29 2004-08-19 Fuji Electric Holdings Co Ltd 抗原の分離装置並びにこれを利用した抗原の計測方法及び装置
JP2005315758A (ja) * 2004-04-30 2005-11-10 Shimadzu Corp マイクロチップ分析方法及び装置
WO2005108571A1 (ja) * 2004-05-07 2005-11-17 Konica Minolta Medical & Graphic, Inc. 検査用マイクロリアクタおよび遺伝子検査装置ならびに遺伝子検査方法
JP2006271350A (ja) * 2005-03-30 2006-10-12 Shimadzu Corp 反応容器処理装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001255328A (ja) * 2000-03-10 2001-09-21 Hitachi Software Eng Co Ltd ハイブリダイゼーション反応検出方法及び検出装置
JP2004233114A (ja) * 2003-01-29 2004-08-19 Fuji Electric Holdings Co Ltd 抗原の分離装置並びにこれを利用した抗原の計測方法及び装置
JP2005315758A (ja) * 2004-04-30 2005-11-10 Shimadzu Corp マイクロチップ分析方法及び装置
WO2005108571A1 (ja) * 2004-05-07 2005-11-17 Konica Minolta Medical & Graphic, Inc. 検査用マイクロリアクタおよび遺伝子検査装置ならびに遺伝子検査方法
JP2006271350A (ja) * 2005-03-30 2006-10-12 Shimadzu Corp 反応容器処理装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HARVEY J.J. ET AL.: "Characterization and applications of CataCleave probe in real-time detection assays", ANALYTICAL BIOCHEMISTRY, vol. 333, no. 2, 15 October 2004 (2004-10-15), pages 246 - 255, XP004573012, DOI: doi:10.1016/j.ab.2004.05.037 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103782157A (zh) * 2011-08-30 2014-05-07 奥林巴斯株式会社 包含胰液的生物试样中的目标粒子的检测方法
CN103782157B (zh) * 2011-08-30 2016-01-20 奥林巴斯株式会社 包含胰液的生物试样中的目标粒子的检测方法
CN104076004A (zh) * 2014-07-16 2014-10-01 常熟理工学院 一种检测样品中汞离子浓度的方法
CN104280373A (zh) * 2014-09-23 2015-01-14 天津科技大学 利用荧光增强法检测二、三价铁离子的方法
WO2022136402A1 (en) * 2020-12-22 2022-06-30 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing

Also Published As

Publication number Publication date
JPWO2008090760A1 (ja) 2010-05-20

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