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WO2008090760A1 - Fluorescent detector, microchip and examination system - Google Patents

Fluorescent detector, microchip and examination system Download PDF

Info

Publication number
WO2008090760A1
WO2008090760A1 PCT/JP2008/050117 JP2008050117W WO2008090760A1 WO 2008090760 A1 WO2008090760 A1 WO 2008090760A1 JP 2008050117 W JP2008050117 W JP 2008050117W WO 2008090760 A1 WO2008090760 A1 WO 2008090760A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescent
detector
microchip
nucleic acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/050117
Other languages
French (fr)
Japanese (ja)
Inventor
Tsuneo Sawazumi
Akihisa Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Konica Minolta Medical and Graphic Inc
Original Assignee
Konica Minolta Medical and Graphic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Konica Minolta Medical and Graphic Inc filed Critical Konica Minolta Medical and Graphic Inc
Priority to JP2008555011A priority Critical patent/JPWO2008090760A1/en
Publication of WO2008090760A1 publication Critical patent/WO2008090760A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

It is intended to provide a fluorescent detector which has a simple constitution and yet enables highly sensitive and highly accurate measurement of fluorescent intensity serving as a label by a technique of detecting a target nucleic acid sequence with the use of fluorescent detection using a hybridization reaction between a target DNA and a fluorescent labeled probe to cause a change in the fluorescent resonance energy transfer, i.e., a technique typified by the cycling probe method; a microchip, and a fluorescent detection system comprising the same. Namely, a fluorescent detector by which a target nucleic acid sequence is detected with the use of a hybridization reaction between a target DNA and a fluorescent labeled probe to cause a change in the fluorescent resonance energy transfer, characterized in that the detector has a light source of excitation light for fluorescent light emission and a section for detecting the fluorescence emitted by the excitation and a longer wavelength side part from the maximum emission wavelength of the emission spectrum of the excitation light at least partly overlaps with the emission spectrum of the fluorescence.
PCT/JP2008/050117 2007-01-26 2008-01-09 Fluorescent detector, microchip and examination system Ceased WO2008090760A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008555011A JPWO2008090760A1 (en) 2007-01-26 2008-01-09 Fluorescence detection device, microchip, and inspection system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007016155 2007-01-26
JP2007-016155 2007-01-26

Publications (1)

Publication Number Publication Date
WO2008090760A1 true WO2008090760A1 (en) 2008-07-31

Family

ID=39644335

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050117 Ceased WO2008090760A1 (en) 2007-01-26 2008-01-09 Fluorescent detector, microchip and examination system

Country Status (2)

Country Link
JP (1) JPWO2008090760A1 (en)
WO (1) WO2008090760A1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103782157A (en) * 2011-08-30 2014-05-07 奥林巴斯株式会社 Method for detecting target particles in biosample containing pancreatic juice
CN104076004A (en) * 2014-07-16 2014-10-01 常熟理工学院 Method for detecting mercury ionic concentration in sample
CN104280373A (en) * 2014-09-23 2015-01-14 天津科技大学 Method for detecting divalent and trivalent iron ions by utilizing fluorescence enhancement method
WO2022136402A1 (en) * 2020-12-22 2022-06-30 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001255328A (en) * 2000-03-10 2001-09-21 Hitachi Software Eng Co Ltd Method and apparatus for detecting hybridization reaction
JP2004233114A (en) * 2003-01-29 2004-08-19 Fuji Electric Holdings Co Ltd Antigen separation apparatus and antigen measurement method and apparatus using the same
JP2005315758A (en) * 2004-04-30 2005-11-10 Shimadzu Corp Microchip analysis method and apparatus
WO2005108571A1 (en) * 2004-05-07 2005-11-17 Konica Minolta Medical & Graphic, Inc. Micro-reactor for testing, genetic testing apparatus, and genetic testing method
JP2006271350A (en) * 2005-03-30 2006-10-12 Shimadzu Corp Reaction vessel processing equipment

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001255328A (en) * 2000-03-10 2001-09-21 Hitachi Software Eng Co Ltd Method and apparatus for detecting hybridization reaction
JP2004233114A (en) * 2003-01-29 2004-08-19 Fuji Electric Holdings Co Ltd Antigen separation apparatus and antigen measurement method and apparatus using the same
JP2005315758A (en) * 2004-04-30 2005-11-10 Shimadzu Corp Microchip analysis method and apparatus
WO2005108571A1 (en) * 2004-05-07 2005-11-17 Konica Minolta Medical & Graphic, Inc. Micro-reactor for testing, genetic testing apparatus, and genetic testing method
JP2006271350A (en) * 2005-03-30 2006-10-12 Shimadzu Corp Reaction vessel processing equipment

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HARVEY J.J. ET AL.: "Characterization and applications of CataCleave probe in real-time detection assays", ANALYTICAL BIOCHEMISTRY, vol. 333, no. 2, 15 October 2004 (2004-10-15), pages 246 - 255, XP004573012, DOI: doi:10.1016/j.ab.2004.05.037 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103782157A (en) * 2011-08-30 2014-05-07 奥林巴斯株式会社 Method for detecting target particles in biosample containing pancreatic juice
CN103782157B (en) * 2011-08-30 2016-01-20 奥林巴斯株式会社 Method for detecting target particles in biological sample containing pancreatic juice
CN104076004A (en) * 2014-07-16 2014-10-01 常熟理工学院 Method for detecting mercury ionic concentration in sample
CN104280373A (en) * 2014-09-23 2015-01-14 天津科技大学 Method for detecting divalent and trivalent iron ions by utilizing fluorescence enhancement method
WO2022136402A1 (en) * 2020-12-22 2022-06-30 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing

Also Published As

Publication number Publication date
JPWO2008090760A1 (en) 2010-05-20

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