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WO2002018541A3 - Sulfotransferase cystosolique specifique du systeme nerveux destinee a l'analyse de medicament - Google Patents

Sulfotransferase cystosolique specifique du systeme nerveux destinee a l'analyse de medicament Download PDF

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Publication number
WO2002018541A3
WO2002018541A3 PCT/US2001/041986 US0141986W WO0218541A3 WO 2002018541 A3 WO2002018541 A3 WO 2002018541A3 US 0141986 W US0141986 W US 0141986W WO 0218541 A3 WO0218541 A3 WO 0218541A3
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WO
WIPO (PCT)
Prior art keywords
sultn
expressed
sulfotransferase
cystosolic
nervous system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/041986
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English (en)
Other versions
WO2002018541A2 (fr
Inventor
David H Farb
Stella Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Boston University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston University filed Critical Boston University
Priority to AU2001295018A priority Critical patent/AU2001295018A1/en
Publication of WO2002018541A2 publication Critical patent/WO2002018541A2/fr
Anticipated expiration legal-status Critical
Publication of WO2002018541A3 publication Critical patent/WO2002018541A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On a identifié SULTn, gène codant pour une nouvelle sulfotransférase du système nerveux, en recherchant dans une base de données de marqueurs de séquence exprimée destinés à la sulfotransférase spécifiquement exprimée dans le cerveau. SULTn est un polypeptide de 33.085 Mr qui structurellement ressemble à des sulfotransférases cystosoliques (identique à 34-39 %, par exemple), avec des motifs structurels de signature de site de liaison de cosubstrat de 3'-phosphoadénosine 5'-sulfotransférase dans le domaine catalytique du polypeptide. Sultn se lie à l'adénosine 3',5'-diphosphate-agarose, analogue du cosubstrat, et des expériences de la concurrence ont démontré la spécificité de cette liaison. A la différence de toutes les sulfotransférases cystosoliques connues, qui sont hautement exprimées dans le foie humain, des transcrits de SULTn de 2,4 kb ont été détectés par analyse de transfert Northern uniquement dans le cerveau humain, mais pas dans le foie, ni dans le coeur, ni dans le placenta, ni dans les poumons, ni dans le muscle squelettique, ni dans le rein, ni dans le pancréas. L'hybridation in situ d'embryons de rat a révélé une expression de développement précoce du gène SULTn dans le système nerveux central, périphérique et entérique. Sultn est largement exprimé dans le système nerveux de l'adulte, y compris dans la couche de cellules de Purkinje du cortex cérébelleux. Le schéma d'hybridation in situ dans le système nerveux était cohérent avec la coloration des neurones, et était différent de l'hybridation de protéine acide fibrillaire gliale, marqueur d'astrocyte. Le gène SULTn humain possède un total de 7 exons codants sur le chromosome 22 (22q13) qui couvre plus de 47kb d'ADN génomique. L'identification d'une nouvelle sulfotransférase cystosolique exprimée exclusivement dans les tissus nerveux révèle ainsi l'existence d'une sulfonation spécifique du système nerveux.
PCT/US2001/041986 2000-09-01 2001-09-04 Sulfotransferase cystosolique specifique du systeme nerveux destinee a l'analyse de medicament Ceased WO2002018541A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001295018A AU2001295018A1 (en) 2000-09-01 2001-09-04 A neural specific cystosolic sulfotransferase for drug screening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22992900P 2000-09-01 2000-09-01
US60/229,929 2000-09-01

Publications (2)

Publication Number Publication Date
WO2002018541A2 WO2002018541A2 (fr) 2002-03-07
WO2002018541A3 true WO2002018541A3 (fr) 2003-04-10

Family

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Family Applications (1)

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PCT/US2001/041986 Ceased WO2002018541A2 (fr) 2000-09-01 2001-09-04 Sulfotransferase cystosolique specifique du systeme nerveux destinee a l'analyse de medicament

Country Status (2)

Country Link
AU (1) AU2001295018A1 (fr)
WO (1) WO2002018541A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7628989B2 (en) 2001-04-10 2009-12-08 Agensys, Inc. Methods of inducing an immune response

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1589985A4 (fr) * 2003-02-04 2007-06-06 Millennium Pharm Inc Procede et compositions pour traiter la douleur et les troubles douloureux en utilisant 16386, 15402, 21165, 1423, 636, 12303, 21425, 27410, 38554, 38555, 55063, 57145, 59914, 94921, 16852, 33260, 58573, 30911, 85913, 14303, 16816, 17827 or 32620
US20060223065A1 (en) * 2003-09-30 2006-10-05 Evotec Neuroscience Gmbh Diagnostic and therapeutic use of a sulfotransferase for neurodegenerative diseases
US7951542B2 (en) 2009-11-04 2011-05-31 Surgene, LLC Methods and compositions for the treatment of psychotic disorders through the identification of the SULT4A1-1 haplotype
US7985551B2 (en) 2009-11-04 2011-07-26 Suregene, Llc Methods and compositions for the treatment of psychotic disorders through the identification of the SULT4A1-1 haplotype
US7951543B2 (en) 2009-11-04 2011-05-31 Suregene, Llc Methods and compositions for the treatment of psychotic disorders through the identification of the SULT4A1-1 haplotype
US7972793B2 (en) 2009-11-04 2011-07-05 Suregene, Llc Methods and compositions for the treatment of psychotic disorders through the identification of the SULT4A1-1 haplotype
CN119752835B (zh) * 2025-01-10 2025-10-14 华中农业大学 一种与茶树中磺基代谢物含量相关的分子标记及其应用

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BIOCHEM. J., vol. 346, no. PT. 2, 3 April 2000 (2000-04-03), pages 857 - 864 *
BIOCHEM. J., vol. 346, no. PT. 3, 3 April 2000 (2000-04-03), pages 857 - 864 *
DATABASE GENBANK [online] 22 June 2001 (2001-06-22), MARTIN ET AL.: "Molecular identificatioon of a rat nervouw system cytoplasmic sulfotransferase, NST", XP002957205, Database accession no. (AF176343) *
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE (BERHESDA, MD, USA); 1 January 2000 (2000-01-01), SAKAKIBARA ET AL.: "Molecular cloning, expression and functional characterization of novel sulfotransferase from brain", XP002957201, Database accession no. (AF115311) *
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE (BETHESDA, MD, USA); 12 December 1999 (1999-12-12), BURGESS J., XP002956900, Database accession no. (Z97055) *
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE (BETHESDA, MD, USA); 22 June 2001 (2001-06-22), MARTIN ET AL.: "Molecular identification of a human nervous system cytoplasmic sulfotransferase, NST", XP002957204, Database accession no. (AF176342) *
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE (BETHESDA, MD, USA); 9 August 2000 (2000-08-09), WALTHER ET AL.: "Molecular and physical characterization of human SULT4A1, representing a novel cytosolic sulfotransferase family", XP002957202, Database accession no. (AF251263) *
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE (BETHESDA, MD, USA); FALANY ET AL.: "Molecular cloning and expression of novel sulfotransferase-like cDNAs from human and rat brain", XP002957203, Database accession no. (AF188698) *
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE (BETHESDA, MD, USA); FALANY ET AL.: "Molecular cloning and expression of novel sulfotransferase-like cDNAs from human and rat brain", XP002957206, Database accession no. (AF188699) *
FALANY ET AL.: "Molecular cloning and expression of novel sulfotransferase-like cDNAs from human and rat brain", BIOCHEMICAL JOURNAL, vol. 346, no. PART 3, 15 March 2000 (2000-03-15), pages 857 - 864, XP002957207 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7628989B2 (en) 2001-04-10 2009-12-08 Agensys, Inc. Methods of inducing an immune response
US7641905B2 (en) 2001-04-10 2010-01-05 Agensys, Inc. Methods of inducing an immune response

Also Published As

Publication number Publication date
AU2001295018A1 (en) 2002-03-13
WO2002018541A2 (fr) 2002-03-07

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