WO1999001221A1 - Micro-reaction vessel, arrangement of micro-reaction vessels and method for discharging a liquid therefrom - Google Patents

Abstract

The invention relates to a micro-reaction vessel, an arrangement of micro-reaction vessels and to a method for discharging a liquid contained in a micro-reaction vessel or arrangement of micro-reaction vessels. The bottom of the micro-reaction vessel is designed in such a way as to create an outlet solely due to the effect of an external force e.g. the impact of compressed air and/or by supplying local heat possibly by means of an electric resistance heater, and to enable a liquid in the reaction chamber(3) to be discharged. The outlet duct is therefore closed by a stopper or by a layer (23) located on the underside(21) of the bottom and acting as a burst plate. It is also possible to include notches in the outlet duct as break-points or indents in the outlet duct. One advantage of the invention is that devices such as pipettes are not required to transfer the contents of the micro-reaction vessel. The invention also relates to arrangements(30) of micro-reaction vessels in a row or in lines and columns in the form of a matrix similar to known titer plates. The arrangement can also comprise liquid-connected micro-reaction vessels which are joined to each other by means of junction canals.

Description

MICRO REACTION TUBE, ARRANGEMENT OF MICRO REACTION TUBE AND METHOD FOR DISPENSING A LIQUID FROM THE SAME

description

The invention relates to microreaction vessels, their arrangements and a method for dispensing a liquid from a microreaction vessel or an arrangement of microreaction vessels.

In biochemistry and pharmaceutical active ingredient research, especially using the concepts of combinatorial chemistry, the smallest amounts of biochemically active substances are converted or tested for their effectiveness. The aim is to implement a large number of samples in the shortest possible time, which is associated with a minimal number of work steps with simultaneous automation.

For the implementation of a large number of small amounts of material, titer plates are currently used which have depressions into which substances are pipetted and react with one another. To transfer the reaction products into other titer plates, for example, it is necessary to take up the contents of the wells in pipettes. It is also known to move thin rods into the wells of the titer plate in order to transfer the adhering substances to other vessels. However, the use of such additional devices, such as pipettes or rods, for transferring the reaction products from one titer plate to another has disadvantages. Additional devices mean additional work steps, a risk of sample contamination and a loss of sample quantity.

For this purpose, a Tiier plate is described in WO 97/15394, the recesses of which have a comparatively large filling opening and a small outlet opening. The surface tension prevents the liquid from flowing out. The liquid can be discharged through the outlet opening by means of a gas pressure surge.

WO 92/02303 describes a titer plate, the recesses of which are closed off by a porous membrane which retains the liquid. The liquid is only dispensed when a gas pressure surge is applied.

A disadvantage of such titer plates is that the dissolved components crystallize out to close the outlet openings or the membrane pores can lead. Another disadvantage is that the dimensioning of the outlet openings or the membrane pores depends on the surface tension of the respective liquid.

The object of the invention is to provide microreaction vessels and arrangements of such microreaction vessels which allow a liquid to be dispensed in a simple manner without having to use devices for this purpose which come into contact with the contents of the microreaction vessel and which do not have the aforementioned disadvantages of known titer plates exhibit. Furthermore, it is an object of the invention to provide a corresponding method for dispensing a liquid from a microreaction vessel or an arrangement of microreaction vessels.

This object is achieved with a microreaction vessel according to the features of claim 1, with arrangements of microreaction vessels according to the features of claim 17 and 22 and with a method according to the feature of claim 23. Advantageous refinements are the subject of the dependent claims.

The bottom of the microreaction vessel comprising at least one filling opening and a reaction chamber is designed such that at least one outlet opening can be produced by the action of an external force and / or local heat supply.

This solution is based on the general idea of the invention that the microreaction vessel initially has no outlet openings, and that at least one outlet opening is only formed by the action of an external force and / or local heat supply.

With the microreaction vessel according to the invention it is therefore possible to add various substances, preferably liquids, metered through the filling opening, to react with one another in the reaction chamber and to dispense a depression, for example, into a titer plate located under the microreaction vessel through the outlet opening to be produced. This partial or complete transfer of the contents of the microreaction vessel does not require a device that comes into contact with the contents of the microreaction vessel. A risk of contamination and the loss of the contents of the microreaction vessel is minimized. Since the outlet opening is only being created, there is also no problem of closure by crystallization.

According to a preferred embodiment, the outlet opening can be generated as an external force by a gas pressure surge introduced via the filling opening. When the gas pressure surge is initiated and the outlet opening is formed, the liquid in the chamber is also partially or completely released. A type of stamp can also be provided as the external force, which preferably engages on the underside of the bottom of the microreaction vessel.

According to a first advantageous variant, at least one outlet channel located in the bottom of the microreaction vessel is closed by a material serving as a plug, which can be removed by the action of a force such as a blast of compressed air. Plastic, elastic or highly viscous materials are preferably provided for this purpose.

According to a second advantageous variant, the microreaction vessel has at least one outlet channel which is covered with a thin layer on the underside of the bottom of the microreaction vessel. The entire underside of the microreaction vessel can also be covered with this layer. This layer can be held on the underside of the bottom of the microreaction vessel by adhesive bonding, fusing or by adhesive forces. The thin layer can advantageously be an integral part of at least the bottom of the microreaction vessel. A thin layer thinner than 100 μm, in particular thinner than 10 μm, is preferred. The action of a force, for example a blast of compressed air, destroys the thin layer in the manner of a rupture disk or detaches it from the underside of the bottom of the microreaction vessel, so as to produce the outlet opening. However, it is also conceivable for the base to be deflected to such an extent by a stamp engaging on the underside of the base that the thin layer tears. The stamp is advantageously designed in this case, for example as a hollow cylinder, and the point of application selected so that the stamp does not come into contact with the contents of the reaction chamber. According to a third embodiment variant, the underside and / or the top of the bottom of the microreaction vessel have one or more predetermined breaking points, for example notches. At these points, one or more outlet openings open under the action of a force, for example by means of a plunger engaging from below.

A fourth embodiment provides that the bottom of the microreaction vessel has one or more incisions at the location of the outlet opening to be created, which cut through the bottom from the bottom to the top partially or completely. Under the action of force from the inside of the microreaction vessel on the top of the bottom, the bottom bulges, as a result of which the outlet opening is opened in the form of a gap in the region of the incision. It is also conceivable to apply force to the underside of the bottom of the microreaction vessel, for example by means of a hollow cylinder as a stamp, to arch the bottom in the direction of the chamber of the microreaction vessel and thus to produce the outlet opening. If the material of the floor has elastic properties, the outlet opening can be opened and closed reversibly.

According to a further embodiment, the outlet opening can be generated by local heat supply by means of electrical energy. This can be supported by an external force, for example a gas pressure surge introduced via the filling opening.

For this purpose, a first embodiment variant provides that the microreaction vessel in the region of the opening to be produced has two electrodes, which are arranged on the underside of the base or in the base and are spaced apart from one another and form an electrical spark gap. By applying a sufficiently high voltage, a spark is formed between the two electrodes and thus a rapid, strong temperature increase in this area.

In a second embodiment variant, at least one heating resistor is arranged on the underside of the floor or in the floor itself in the region of the opening to be produced. The electrical heating resistor is connected to electrical leads. When an electrical current flows through the Heating resistance there is a local temperature increase in the bottom of the microreaction vessel.

The electrodes, heating resistors or electrical leads can advantageously be applied by means of thin-film techniques, for example printed on the bottom of the microreaction vessel or the arrangement of microreaction vessels.

An outlet opening can be produced in a simple manner by local heat supply, preferably according to one of the two design variants described above, in connection with one of the four design variants listed above for designing the bottom of the microreaction vessel.

For example, the outlet channel can be closed by a material serving as a plug, which can be softened when heated and, if necessary supported by a gas pressure surge, can be removed from the outlet channel.

In connection with the embodiment variant, which provides a thin layer covering the outlet channel, this layer is preferably designed in such a way that it cracks, melts or decomposes at this point when heat is applied. This can be a thin plastic layer, for example. In connection with the third embodiment variant, this can be supported by predetermined breaking points.

The fourth embodiment variant with incisions in the bottom of the microreaction vessel can also be combined with the embodiment that provides for local heating. For example, the floor in the area of the incisions can be designed such that an outlet opening in the form of a gap is opened when heated by thermal expansion.

The reaction chamber of the microreaction vessel can be designed in such a way that it has approximately the same diameter as the filling opening. Thus, a cylindrical shape of the chamber can be provided, which tapers conically at the end opposite the filling opening to the diameter of the outlet channel. The reaction chamber can also have a larger diameter than the filling opening. So has a spherical shape Chamber the advantage of less evaporation and a reduced risk of splashing when adding liquids.

Filling openings with a diameter of 100 μm to 2 mm are preferred for aqueous systems. Such microreaction vessels can have, for example, a chamber volume of 1 μl to 500 μl. Depending on the application, other dimensions for the diameter and chamber volume can also be used.

For some liquids to be dosed, it may be advantageous for droplet formation if the outlet opening to be created is formed into a tip. It can also be advantageous if the outlet opening widens outwards in a conical manner.

Furthermore, a sieve or a filter can be fitted in the chamber or between the chamber and the outlet channel, so that when the chamber is acted upon by a blast of compressed air with the discharge of the liquid through the outlet opening, solid components are simultaneously separated.

The microreaction vessel preferably consists of a material which is chemically inert to the liquids used and which is biocompatible with the biochemical substances used. For example, plastics which include, for example, polycarbonate, polyethylene ether ketone, cyclic olefin copolymers, polymethyl methacrylate or mixtures thereof are suitable.

The microreaction vessel is preferably a one-piece molded body made of plastic. The use of microtechnologically manufactured mold inserts during manufacture, for example in the injection molding process, enables the high precision required.

In order to allow a simple connection of the microreaction vessels to one another, these advantageously have connecting elements, such as plug-in and / or locking elements.

The independent claim 17 relates to an arrangement of several microreaction vessels according to the invention side by side in a row or in rows and columns in the form of a matrix. With such arrangements, a large number of samples can be handled simultaneously.

The matrix-like arrangement advantageously has the same number of chambers, for example 96, 384 or 864, and the same external dimensions as standard titer plates. This enables automated handling using commercially available devices.

The arrangement can consist of individual microreaction vessels or arrangements, such as rows, of microreaction vessels which are connected to one another in a detachable or non-detachable manner. A detachable connection is made possible, for example, via latching and / or plug-in elements. An inseparable connection is achieved by gluing or welding. According to another variant, the microreaction vessels are an integral part of the arrangement. For example, the arrangement can consist of a single molded body produced by injection molding.

This arrangement can also include microreaction vessels which have electrical heating resistors arranged in the region of the outlet openings to be produced on the underside of the base or in the base itself or electrodes spaced apart from one another, each forming an electrical spark gap. For this purpose, the arrangement has electrical contacts which are connected to the heating resistors or electrodes via electrical leads. If the arrangement is a titer plate, these electrical contacts can be arranged, for example, on the side, on the top or on the bottom. Individual outlet openings can thus be produced in a targeted manner via an electrical control unit.

The further arrangement according to the invention according to claim 22 has at least one microreaction vessel according to the invention and at least one further microreaction vessel which comprises a filling opening and a reaction chamber, the microreaction vessel according to the invention and the further microreaction vessel each having at least one lateral connection channel via which the at least two microreaction vessels are fluidly connected to one another are connected. Due to the fluidic connection of at least two microreaction vessels, reactions can also be carried out in several steps with several reaction partners in a single arrangement of microreaction vessels. In this way, intermediates for inclusion in subsequent reactions can be transferred from one microreaction vessel to another via the side connection and reacted there with another starting material. The contents of the reaction chamber can then be dispensed from the microreaction vessel according to the invention in accordance with one of the methods described above, for example by means of a gas pressure surge. The transfer of the contents of one reaction chamber into another via lateral connecting channels can also be carried out via a gas pressure surge.

Here, different embodiments can be implemented, which differ in the number and type of arrangement of the connecting channels, outlet openings and the shape of the chamber. Arrangements are preferred in which the connecting channels open into the lower region of the reaction chamber, so as to enable the reaction chamber to be completely emptied.

The method for dispensing a liquid from a microreaction vessel or an arrangement of microreaction vessels provides, according to claim 23, that at least one outlet opening is created by the action of an external force and / or by local heat supply. The microreaction vessels and their arrangements according to claims 1 to 22 are particularly suitable for this.

A gas pressure surge is preferably provided as the external force, which creates the outlet opening and at least partially releases the liquid.

The generation of an outlet opening by local heating is preferably achieved by softening, melting or destroying a thin plastic layer. For this purpose, the heat is advantageously supplied locally by an electrical resistance heater or an electrical discharge.

Exemplary embodiments are explained in more detail below with reference to the schematic drawings: FIG. 1 shows a microreaction vessel with a plug in the outlet channel in cross section from the side,

Figure 2 shows a microreaction vessel with a thin layer on the underside of the

Floor in cross section from the side,

FIG. 3 shows a microreaction vessel in which the thin layer is an integral part of the bottom, in cross section from the side,

Figure 4 shows a microreaction vessel with predetermined breaking points in the bottom in cross section of the

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Figure 5a shows a microreaction vessel with an incision in the bottom in cross section of the

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5b shows the microreaction vessel according to FIG. 5a in cross section from above.

FIG. 6 shows a matrix-like arrangement of microreaction vessels in perspective

Representation cut from the side,

7a-c three fluidly connected to one another via connecting channels

Microreaction vessels in cross section from the side,

FIG. 8 shows a microreaction vessel with an electrical heating resistor in cross section from the side, 9a and 9b a microreaction vessel with an electrical heating resistor in a top view from below,

FIG. 9c shows a microreaction vessel with spaced electrodes in a top view from below,

Figure 10 shows a microreaction vessel with spaced electrodes in cross section from the

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11a shows an arrangement of 3x3 microreaction vessels with electrical contacts in a perspective view,

Figure 11 b shows the arrangement of Figure 11a in plan view from below.

FIG. 1 shows a microreaction vessel 1 in which the bottom has an outlet channel 4 connecting the upper side 20 to the lower side 21, a plug 22 being located in the outlet channel 4. The plug 22 can be pushed out to the underside 21 of the base by a gas pressure surge introduced via the filling opening 2, whereby an outlet opening 5 is created and a liquid in the chamber 3 is dispensed.

In Figure 2, the outlet channel 4 is closed by a thin layer 23 located on the underside 21 of the bottom of the microreaction vessel 1, which can be, for example, a laminated thin plastic film. This layer 23 can also be an integral part of the floor, as shown in FIG. 3. The layer 23 is preferably so thin that it is destroyed in the manner of a rupture disk under the action of, for example, a gas pressure surge, and an outlet opening is thus formed.

FIG. 4 shows a microreaction vessel 1 with notches 24 in the top 20 and the bottom 21 of the bottom. These indentations 24 surrounding a central region 26 represent predetermined breaking points. Under the action The central region 26 can be separated from the rest of the floor by an external force, as a result of which an outlet channel 4 with an outlet opening 5 is formed.

A microreaction vessel with two incisions 25 penetrating the bottom from the top 20 to the bottom 21 is shown in FIGS. 5a and 5b. FIG. 5a shows the microreaction vessel in cross section from the side, a section 25 being visible. Figure 5b shows the top 20 of the bottom of the microreaction vessel with two intersecting incisions 25 and the wall 6 of the microreaction vessel 1 in section from above. By the action of a force from one side of the base, the base bulges to the other side with the release of a gap serving as the outlet channel 4 along the incisions 25 and thus with the formation of an outlet opening 5.

A one-piece matrix-like arrangement 30 of microreaction vessels is shown in FIG. 6, a row of microreaction vessels 1 being shown in cross section from the side. Such microreaction vessels arranged in the manner of known titer plates allow simple handling of a large number of substances under the same conditions. The microreaction vessels shown here correspond to the microreaction vessel according to FIG. 2 with a thin layer 23 located on the underside 21 of the bottom. However, other microreaction vessels according to the invention can also be used for such an arrangement. The contents of individual microreaction vessels can be released, for example, by targeted pressurization of individual filling openings 2 with compressed air blasts. For this purpose, the thin layer in the area of the respective outlet channel 4 is destroyed in the manner of a rupture disk by the compressed air blast.

An arrangement of three microreaction vessels 1, 10a, 10b is shown in FIGS. 7a-c. The middle microreaction vessel 1 represents a microreaction vessel according to the invention in the manner of a microreaction vessel according to FIG. 2 with a thin layer 23 on the underside 21 of the bottom. This is via additional connecting channels 7 and 9 with the two connecting channels 14 and 13 of the laterally arranged microreaction vessels 10a , 10b connected. Furthermore, the middle microreaction vessel 1 has an outlet channel 4 at the bottom of the reaction chamber 3, which leads to the thin layer 23 located on the underside of the middle microreaction vessel 1. Substances or liquids which react with one another in the chamber 12a can be added via the filling opening 11a of the microreaction vessel 10a arranged on the left in FIG. 7a. By acting on the chamber 12a of the left microreaction vessel 10 with a blast of compressed air P, which is indicated here as a large arrow above the filling opening 11a, the contents of the chamber 12a can be conveyed into the chamber 3 of the central microreaction vessel 1 via the connecting channels 13, 9. The direction of flow is indicated by a small arrow below the microreaction vessel 10a. In this case, it may be advantageous to also apply a blast of compressed air to the right microreaction vessel 10b at the same time.

If the left microreaction vessel 10a is simultaneously pressurized via the filling opening 11a and the middle microreaction vessel 1 via the filling opening 2, the contents of the left microreaction vessel 1 can be conveyed into the middle microreaction vessel 1 and from there into the right microreaction vessel 10b (FIG 7b). If all three microreaction vessels 1, 10a, 10b are acted upon by a blast of compressed air through the filling openings 2, 11a, 11b, the contents of the central microreaction vessel 1 can be dispensed via the outlet channel 4 to form an outlet opening 5 in the thin layer 23 (FIG. 7c).

The microreaction vessels 1, 10a, 10b can be detachably connected to one another, for example with the aid of plug-in elements in the manner of tongue and groove. The microreaction vessels 1, 10a, 10b can also be permanently connected to one another or can be produced as a one-piece molded body, preferably made of a plastic.

FIGS. 8 to 11 b show microreaction vessels and their arrangements in which an outlet opening can be generated by local heat supply, here in the form of electrical energy.

FIG. 8 shows a microreaction vessel 1 in cross section from the side, the underside 21 of the bottom of which has a heating resistor 40 connected to electrical leads 41a, 41b. The heating resistor 40 is located in the region of the outlet channel 4, which opens out on a thin layer 23. When current flows through the heating resistor heats up this thin layer, depending on the material used at this point tears open, melts or decomposes. This can be supported by a gas pressure surge, which at the same time pushes out at least part of the liquid in the reaction chamber 3 through the outlet channel 4 and the outlet opening, not shown here. The underside of this microreaction vessel is shown in Figure 9a. The heating resistor 40 can be made of the same material as the feeds 41 a, 41 b, for example by means of thin film technology. By reducing the cross section compared to the feeds 41 a, 41 b, a higher resistance is achieved.

Another embodiment of the electrical heating resistor 40, in the form of two semicircles surrounding a central region, is shown in FIG. 9b. The outlet opening to be produced lies in this middle region, in which the highest temperatures are reached when heated.

In FIG. 9c, the electrical feeds are designed as two electrodes 43a, 43b, which form an electrical spark gap 42 due to their spacing. If the voltage is sufficiently high, an electrical discharge takes place between the opposing electrodes 43a and 43b, as a result of which the floor is locally heated in the region of the gap 42.

A further variant of two spaced electrodes 43a, 43b forming a spark gap 42 is shown in FIG. In contrast to the exemplary embodiment according to FIG. 9c, the electrodes are arranged in the bottom of the microreaction vessel. This enables a targeted heating of the material in the region of the outlet opening to be produced.

An arrangement of 3 × 3 microreaction vessels is shown in FIGS. 11 a and 11 b, the outlet openings of which can be generated by means of local heat supply by electrical energy. FIG. 11b, in which the position of the reaction chambers 3 is indicated by broken circles, shows the arrangement with a view of the underside 21. On the underside 21, the electrical leads 44a, 44b,.. connect the laterally arranged electrical contacts 45. This arrangement makes it possible to electrically control each individual heating resistor by means of an electrical control unit, not shown here, and thus to produce individual outlet openings in a targeted manner. Reference numerals

- Microreaction vessel filling opening reaction chamber outlet channel outlet opening wall side connecting channel side connecting channel a, 10b microreaction vessel a, 11b filling opening a, 12b reaction chamber side connecting channel side connecting channel top of the bottom underside of the bottom grafting thin layer notch incision middle area arrangement of microreaction vessels arrangement of fluidly connected microreaction vessels Arrangement of microreaction vessels with electrical heating elements electrical heating resistor a, 41 b electrical supply gap a, 43b electrode a, 44b electrical supply electrical connection contacts

Claims

claims Microreaction vessel (1) with at least one filling opening (2) and a reaction chamber (3), the bottom of which is designed such that at least one outlet opening (5) can be produced by the action of an external force and / or by local heat supply. Microreaction vessel according to claim 1, characterized in that the outlet opening (5) can be generated by a gas pressure surge introduced via the filling opening (2). 3. Microreaction vessel according to claim 1 or 2, characterized in that the microreaction vessel (1) has at least one outlet channel (4) which is at least partially closed with a material serving as a plug (22). Microreaction vessel according to claim 1 or 2, characterized in that the microreaction vessel (1) has at least one outlet channel (4) which is covered with a thin layer (23) on the underside (21) of the bottom of the microreaction vessel. Microreaction vessel according to claim 4, characterized in that the thin layer (23) covers the entire underside (21) of the bottom of the microreaction vessel (1). Microreaction vessel according to claim 4, characterized in that the thin layer (23) is an integral part of at least the bottom of the microreaction vessel (1). Microreaction vessel according to one of claims 4 to 6, characterized in that the thin layer (23) has a thickness of less than 100 μm, preferably less than 10 μm. 8. microreaction vessel according to claim 1 or 2, characterized in that the top (20) and / or the bottom (21) of the bottom has one or more, the position of the outlet opening (5) predetermined predetermined breaking points (24). Microreaction vessel according to claim 1 or 2, characterized in that the bottom at the location of the outlet opening (5) to be formed has at least one cut (25) which at least partially cuts through the bottom from the bottom (21) to the top (20). 10. Microreaction vessel according to one of claims 1 to 9, characterized in that the microreaction vessel (1) in the region of the outlet opening to be produced two on the underside (21) of the bottom or in the bottom, spaced from one another, forming an electrical spark gap (42) Has electrodes (43a, 43b). 11. Microreaction vessel according to one of claims 1 to 9, characterized in that the microreaction vessel (1) in the region of the outlet opening to be produced has at least one electrical heating resistor (40) arranged on the underside (21) of the base or in the base, the electrical Heating resistor with electrical leads (41a, 41 b) is connected. 12. Microreaction vessel according to one of the preceding claims, characterized in that the filling opening (2) has a diameter of 100 μm to 2 mm. 13. Microreaction vessel according to one of the preceding claims, characterized in that the reaction chamber (3) has a volume of 1 ul to 500 ul. 14. Microreaction vessel according to one of the preceding claims, characterized in that a sieve or a filter is located in the reaction chamber (3) or between the reaction chamber (3) and the outlet opening (5) to be produced. 15. Microreaction vessel according to one of the preceding claims, characterized in that the microreaction vessel (1) has connecting elements by means of which microreaction vessels can be laterally connected to one another. 16. Microreaction vessel according to one of the preceding claims, characterized in that the microreaction vessel is a one-piece molded body made of plastic. 17. Arrangement (30) of several microreaction vessels (1) according to one of claims 1 to 16 side by side in a row or in rows and columns in the form of a matrix. 18. An arrangement (30) according to claim 17, characterized by microreaction vessels (1) arranged in the manner of a microtiter plate. 19. The arrangement according to claim 17 or 18, characterized in that the arrangement (30) comprises individual microreaction vessels (1) or arrangements of microreaction vessels (1) which are detachably or non-detachably connected to one another. 20. The arrangement according to claim 17 or δ, characterized in that the microreaction vessels (1) are an integral part of the arrangement (30). 21. Arrangement (32) according to one of claims 17 to 20, characterized in that the arrangement in the region of the outlet openings to be produced on the underside (21) of the bottom or in the bottom arranged electrical heating resistors (40) or spaced apart, each an electrical Has spark gap (42) forming electrodes (43a, 43b), the electrical heating resistors or the electrodes being connected via electrical leads (44a, 44b, ...) to electrical connection contacts (45) arranged on the arrangement (32). 22. Arrangement (31) of at least one microreaction vessel (1) according to one of claims 1 to 16 and at least one further microreaction vessel (10a, 10b), which has a filling opening (11a, 11b) and a reaction chamber (12a, 12b), thereby characterized in that the microreaction vessel (1) and the further microreaction vessel (10a, 10b) each have at least one lateral connecting channel (7, 9, 13, 14) via which the at least two microreaction vessels (1, 10a, 10b) are fluidly connected to one another are. 23. Procedure for dispensing a liquid from a Microreaction vessel or an arrangement of microreaction vessels in which at least one outlet opening is produced by the action of an external force and / or by local heat supply. 24. The method according to claim 23, characterized in that the outlet opening is generated by a gas pressure surge and the liquid is at least partially discharged. 25. The method according to claim 23, characterized in that in A thin plastic layer is softened, melted or destroyed by local heat supply in the area of the outlet opening to be produced. 26. The method according to claim 25, characterized in that local heat is supplied by an electrical resistance heater or an electrical discharge.