WO2000035583A1 - Method for releasing a fluid, fluidic element and a device for handling such elements - Google Patents

Abstract

Fluidic elements which comprise cavities for accommodating fluids and which can be opened according to need in order to release the fluid are required for biotechnological applications or in the field of active substance research. According to the inventive method, a fluid located in a cavity is released by using laser radiation to destroy a wall, said wall delimiting the cavity, in the area to be opened. In the fluidic element, the material of the wall which delimits the cavity is selected and the wall is configured such that an area of the wall can be destroyed using laser radiation in order to open the cavity. This is advantageous in that the fluid located inside the cavity is reliably prevented from escaping when the cavities are subjected to external forces such as external pressure or centrifugal forces. The inventive method enables the targeted connection of cavities in complex fluidic systems. A device is also provided for handling the described elements.

Description

 Method for dispensing a fluid, fluidic component and device for handling such components

The invention relates to a method for dispensing a fluid according to the preamble of claim 1, a fluidic component and a device for handling fluidic components.

Fluidic components with at least one cavity for holding fluids are increasingly used in biotechnology, combinatorial chemistry and in high-throughput screening in drug discovery. Fluidic components can be, for example, microreaction vessels, titer plates, analysis systems integrated on one component or components for capillary electrophoresis. Such cavities in particular have chambers and channels. Frequently, there is a requirement that such a cavity should first be closed, for example for storing substances or for carrying out a chemical reaction, and that the cavity should later be opened or connected to another cavity, for example in order to dispense a substance or a substance to continue processing.

Titer plates are known from WO 97/15394, in which the individual cavities designed as depressions each have a small opening in their base. A liquid in a cavity can be dispensed through the small opening by a gas pressure pulse. The disadvantage here is that the size of the opening has to be matched to the surface tension of the liquid used. Furthermore, crystallization associated with the evaporation of the liquid can lead to clogging of the small opening.

EP 0 329 120 A2 proposes an arrangement of reaction vessels in the manner of a titer plate, with small openings being provided with a plug in the bottoms of the cavities after an embodiment are closed. Here, too, the plug can be pressed out and the liquid can be dispensed by applying a gas pressure pulse. It is disadvantageous here that metered delivery is not readily possible due to the pressure to be built up to remove the plug. In addition, the graft also released could interfere with further handling.

A device for handling such titer plates with a device for the targeted application of a gas pressure pulse is proposed in WO 92/02303.

WO 97/21090 proposes integrated systems for microanalysis or microsynthesis as fluidic components in which a liquid is moved in defined channels by centrifugal forces. For this purpose, the component is rotated around an axis. The component, preferably a disc, has channels, reagents, reservoirs, reaction chambers, detection chambers, and inlets and outlets. In addition, active systems, such as heat exchangers, electrophoresis or analysis systems, can also be provided. For the selective opening of cavities, pages 24 - 28 mention piezoelectric, pneumatic, electrostatic valve types. However, such valves require an electrical or pneumatic connection, which is not easy to implement due to the rotation of the component. In addition, valves that switch by relaxing polymeric structures that are not in equilibrium are proposed. Such valves switch when heated locally, for example by means of a laser. Since several materials have to be used and a structure has to be fixed in the non-equilibrium state, the production is relatively complex. Finally, valves with constrictions in capillary-like channels are also mentioned. Here, however, there is a dependency on the surface tension of the liquid used. The object of the invention is therefore to provide a method by means of which a fluid located in a cavity of a fluidic component can be dispensed through targeted, simple and contactless opening of at least a region of a wall delimiting the cavity without active elements within the fluidic Component are required. It is also an object of the invention to demonstrate a fluidic component that enables this method. Finally, the object of the invention is to propose a corresponding device for handling such fluidic components.

The object is achieved by a method according to claim 1, by a fluidic component according to claim 13 and by a device according to claim 28. Subclaims relate to advantageous embodiments of the invention.

According to the method, a fluid located in a cavity of a fluidic component is released, in which a wall delimiting the cavity is opened at least in one area. For this purpose, the area of the wall to be opened is at least partially destroyed by supplying energy by means of electromagnetic radiation, preferably laser radiation.

By means of electromagnetic radiation, in particular laser radiation, of sufficient energy density, an opening can be created in a targeted and contactless manner in the wall, which delimits the cavity in which the fluid is located.

The use of laser radiation to produce the finest structures and holes is known. For example, WO 97/15394 mentions that holes can be produced in the bottoms of the recesses in a titer plate using a laser. Such titer plates, in which each recess has a hole in the bottom, can hold liquids, the liquid not flowing out due to the surface tension. By targeting one Gas pressure pulse, the liquid can be released through the hole. All holes are therefore made in the titer plate during manufacture.

In contrast to this, according to the method according to the invention, an opening is specifically created only when the fluidic component is used, in order to release a fluid located in a cavity. The fluid is initially prevented from flowing out through the closed wall. The surface tension of the fluid and a pressure prevailing in the cavity play no role here. A region of the wall is deliberately destroyed by means of preferably a laser beam and an opening is thus created so that the fluid can flow out.

Liquids, gases and multiphase systems, such as dispersions or emulsions, are suitable as the fluid. Particularly suitable fluidic components are described in the claims 13 to 27. At least the area of the wall delimiting the cavity advantageously has a thickness of <500 μm, preferably <100 μm.

Electromagnetic radiation of at least one wavelength is advantageously used, which is absorbed by at least the region of the wall that is to be opened. The preferred wavelength range is from 100 nm to 12000 nm, particularly preferably from 300 nm to 2000 nm. Both suitable radiation sources, for example infrared diode lasers, fiber lasers, Nd: YAG lasers, excimer lasers or CO _2, can be used in these ranges - Find lasers as well as suitable materials that absorb in this area. The laser can be operated continuously or in pulsed mode. The area to be opened is destroyed by melting, evaporation, sublimation, decomposition and / or internal mechanical stresses of the wall material caused by the thermal load. For the sake of simplicity, only the terms laser beam, laser radiation and laser are used in the following, whereby the more general meaning of electromagnetic beam, electromagnetic radiation or electromagnetic radiation source is always to be understood. The laser radiation is advantageously focused on the area to be opened. However, it is also possible to work without focusing, for example with sufficient energy density by means of collimated laser radiation.

The method is particularly suitable for dispensing a fluid, in particular a liquid, from a fluidic component which has a plurality of cavities designed and arranged in the manner of the recesses in titer plates for receiving, chemically converting and / or storing fluids. In particular, titer plates with a sufficiently thin bottom are suitable, the bottom preferably consisting of a polymeric material.

According to a variant of the method, which is used, for example, in the case of titer plates, an opening is created in an outwardly bordering wall delimiting a cavity, as a result of which a fluid located in the cavity is released from the fluidic component to the outside.

According to a second variant of the method, the fluid is dispensed into a second cavity of the same fluidic component separated from the first cavity by the wall. A fluid located in a first cavity can thus be transferred into a second cavity of the fluidic component, the two cavities initially being separated from one another by a wall.

In order to reach the wall to be opened with the laser beam, it can be advantageous to guide the laser beam in such a way that part of the fluidic component is penetrated before the laser beam hits the area of the wall to be opened. For this purpose, a fluidic component is preferably used, which is largely transparent to the radiation used, at least in the part to be penetrated by the laser beam. However, it is also possible for the wall to be opened to have the same material as the part of the fluidic component to be penetrated by the laser beam. For this purpose, the laser beam is focused on the area to be opened. An energy density is thus achieved in the focused area, which enables a region of the wall to be destroyed, while the part to be penetrated by the laser beam lies in a widened beam area, so that no destruction takes place here due to the reduced energy density.

The focusing can be achieved by a lens system attached in the beam path. By changing the position of the focus, areas lying one behind the other in the laser beam can also be deliberately destroyed, thus opening up different cavities.

When penetrating a part of the fluidic component, the laser radiation is advantageously focused on the area to be opened. For this purpose, areas of the fluidic component are shaped and arranged in such a way that the beam is focused. This can be achieved, for example, by lens systems integrated into the component or embossed microlenses.

In the simplest case, the fluid in the cavity is released through the open wall due to gravity. For this purpose, an external pressure is advantageously applied. For example, gas pressure pulses allow a defined amount of liquid to be dispensed from the cavity. According to another variant, the fluid is released from the cavity when the fluidic component rotates about an axis due to the centrifugal forces that occur. This method is described in detail in WO 97/21090 and in the literature cited therein.

It is therefore particularly advantageous of the method according to the invention that the cavities are initially closed, so that external forces, such as external pressure or centrifugal forces, only release the fluid when the walls delimiting the cavities are deliberately opened. In a fluidic component, a plurality of areas to be opened that are located in one or different planes can be provided. In order to be able to open these areas in a targeted manner, the fluidic component and the laser or / and the laser beam are positioned relative to one another. For this purpose, for example, a mirror system can be provided that allows the laser beam to be deflected onto any areas of the fluidic component. However, the laser can also be positioned directly. The positioning can also be carried out by rotating the fluidic component, which is also used to transport liquids by centrifugal forces. Provided that the laser pulses are sufficiently short, the method can be carried out on the rotating fluidic component.

The fluidic component according to the invention has at least one cavity for receiving fluids, the material of a wall delimiting the cavity being selected and the wall being such that a region of the wall for opening the cavity by supplying energy by means of electromagnetic radiation, preferably by means of Laser radiation, is destructible. The fluidic component is particularly suitable for carrying out the method according to the invention.

The wall preferably has a thickness of <500 μm, particularly preferably <100 μm, at least in the area to be opened. The fluidic component, parts thereof or at least the wall to be opened advantageously consist of a polymeric material. Thermoplastic materials such as polymethyl methacrylate, polycarbonate, polyoxymethylene, polyethylene or cyclic olefin copolymers are particularly suitable. Using polymeric materials, fluidic components or parts thereof can be produced inexpensively by means of molding processes such as injection molding or stamping. The fluidic component particularly preferably consists of a one-piece polymeric part. Mold inserts produced by means of microtechnical processes, such as spark erosion and / or LIGA, are advantageously used for the production of impression-making microfluidic systems. The fluidic component or however, at least parts thereof can consist of other materials such as glass, quartz glass, metal and / or ceramic.

The material of the wall is advantageously selected, at least in the area to be opened, in such a way that laser radiation in at least part of the wavelength range from 100 nm to 12000 nm, preferably from 300 nm to 2000 nm, is absorbed. Instead of an absorbent material of the wall, a material absorbing the laser radiation can also be applied or integrated on or in the wall, at least in the area to be opened. Thus, particles, for example made of carbon, or a dye can be applied or introduced onto the surface of the wall or into the material of the wall. If the material is only applied or integrated in the area to be opened and the actual material of the wall is largely transparent for the wavelength range in question, the area can be opened in a targeted manner without exact positioning or focusing being required.

So that the laser radiation can penetrate parts of the fluidic component as far as the area to be opened, it is advantageous that the fluidic component is transparent at least in an area around the wall to be opened in a wavelength range in which the wall itself absorbs.

In order to achieve a focusing of the laser beam when penetrating the fluidic component, the component advantageously has at least one element for focusing laser radiation on the area of the wall to be opened. Thus, lens systems can be integrated at the same time during the production of the component, for example microlenses can be produced by hot stamping.

According to one embodiment, the fluidic component has a plurality of cavities arranged next to one another and accessible to one side. The cavities are preferably designed and arranged in the manner of the recesses in a titer plate. According to a further embodiment, the fluidic component has at least two cavities separated from one another by a wall. The cavities can be reaction chambers or channels, for example, which are connected to further channels or chambers. A fluid can thus be released from the first cavity into the second cavity or vice versa by opening the wall.

The fluidic component advantageously has at least two parts, an upper part and a lower part, both parts each having at least one of the cavities, and at least one film separating the two cavities from one another as a wall. The parts can be, for example, plate-shaped parts which have depressions or recesses, a film being arranged between the two parts, so that the depressions or recesses are covered by the foil and thereby defined cavities are formed. At least at one point, two cavities are separated from one another by the film, so that the two cavities are connected to one another by destroying the film in this area by means of the method according to the invention.

At least part of the fluidic component is particularly advantageously transparent in a wavelength range in which the film absorbs. It is thus possible to direct or focus the laser beam through the transparent part onto the area of the film to be opened.

According to another embodiment, the fluidic component has more than two parts, for example an upper, middle and lower part, parts having cavities and at least one film each between parts, for example between the upper and middle part and between the middle and lower part to separate voids. One part each and the adjacent film can also be realized as a one-piece part. With such multilayer designs, complex fluidic systems can be used Realize separate cavities to be connected to one another by the method according to the invention. The cavities, for example channels and chambers, can each extend in one part or over several parts.

The cavities are advantageously arranged in such a way that a liquid in or between the cavities can be moved by applying an external pressure, for example in the form of gas pressure pulses, by gravity or / and when the component rotates about an axis by centrifugal forces. Components and structures for transporting liquids by means of centrifugal forces are described in detail in WO 97/21090 and in the literature cited therein.

Depending on the application, the fluidic component advantageously has cavities designed as channels, reaction chambers, detection chambers, mixer structures, separating structures, branching structures and / or heat exchangers. Such components can be used for storage, for carrying out physical or / and chemical reactions and / or for analysis. Further functional elements, for example sensors, heating sources, active valves, pumps, analysis systems or electrophoresis systems, can be integrated into the fluidic components or connected to them.

Furthermore, the invention relates to a device for handling fluidic components according to the invention. The device has a receptacle for at least one such fluidic component, a positioning device for the relative positioning of the component and at least one laser and / or a laser beam with respect to one another, and a control unit connected to the positioning device and the laser for the targeted delivery of a fluid according to a method of the claims 1 to 12.

According to one embodiment, the device has at least one focusing unit connected to the control unit for focusing the laser beam on the area to be opened. This makes it possible to focus on different areas, in particular on different levels.

In order to transport liquids in cavities by means of centrifugal forces, the device has a drive device for rotating the fluidic component about an axis. This drive can simultaneously be part of the positioning device, for which purpose the drive is connected to the control unit.

If liquids are conveyed by means of external pressure, the device advantageously has a pressure transmitter device connected to the control unit for the targeted supply of gas pressure pulses to corresponding supplies of the fluidic component. This can be, for example, a compressed air source that has a fast-switching valve.

The device advantageously has at least one reading or / and writing unit connected to the control unit for reading out or writing information stored optically and / or magnetically on the fluidic component. For this purpose, the fluidic component has correspondingly readable or writable areas. The fluidic component can be designed in the form of a disk as an additional optical information carrier in the form of a CD-ROM or in the form of a flat chip as an additional magnetic information carrier in the form of a magnetic card.

The device can contain further devices for carrying out analyzes, separations, chemical reactions, sample treatment or sample transfer.

Exemplary embodiments of the invention are explained in more detail below with the aid of schematic drawings. They show a perspective view cut from the side: 1 shows a fluidic component in the manner of a titer plate,

2 shows a disk-shaped fluidic component with an upper and lower part and a film arranged between them,

3 shows a two-part fluidic component with cavities arranged one above the other.

A fluidic component 1 according to the invention is shown schematically in FIG. The cavities 2a, 2b, 2c, ... arranged next to one another and accessible from the top are designed and arranged in the manner of the recesses in a titer plate. Each cavity 2a, 2b, 2c, ... is bounded by a wall 3a, 3b, 3c, ... on the underside. The material of each wall 3a, 3b, 3c, ... is selected in such a way and each wall 3a, 3b, 3c, ... is designed such that it can be destroyed by means of a suitable wavelength and energy density. The fluidic component 1 consists of a one-piece part produced by molding from a thermoplastic polymer material, the walls 3a, 3b, 3c, ... having a thickness of approximately 250 μm. The diameter of each cylindrical cavity 2a, 2b, 2c, ... is approximately 2 mm. The fluidic component 1 serves to hold the smallest amounts of substance, their further chemical conversion and / or storage, for which purpose the top can be covered with a film, for example by lamination, to avoid evaporation. In order to be able to dispense the liquid 8 located in a cavity 2b, an area of the wall 3b is destroyed by means of a laser 5 using the method according to the invention. In FIG. 1, the wall 3a has an opening 4 created by means of the laser 5 as a small hole. By means of a mirror system, here only one mirror 7 and its direction of movement is indicated by a double arrow, the laser beam 6 can be directed onto walls 3a, 3b, 3c,...

For example, the fluidic component 1 can be made from polyparbonate by means of injection molding. A KrF excimer laser with a wavelength of 248 nm with an energy density of 1 J / cm ^{2 can be used} with a pulse duration of about 20 ns.

FIG. 2 shows a fluidic component 10 in which a liquid can be moved by means of centrifugal forces. The disk-shaped component 10 consists of an upper part 11a, a lower part 11b and a film 13 arranged between them. The upper part 11a has a plurality of bores as fluid feeds 20a, 20b, 20c,... The feed 20a is connected to a cavity 12a. The cavity 12a is formed by a recess in the underside of the upper part 11a. The lower part 11b has a recess on its upper side, which forms a cavity 12b and a detection chamber 21 connected to it. In the assembled state, the cavities 12a and 12b coincide at one point, but are separated from one another at this point by the film 13. There is a liquid 18 in the cavity 12a. In order to discharge the liquid 18 from the cavity 12a into the cavity 12b, the laser beam is emitted by means of a laser 15 and a mirror arrangement 17. 16 directed to the opening area of the wall 14 formed by the film 13. The lower part 11b is transparent in the relevant wavelength range in order to allow penetration of the laser beam, while the film absorbs in the wavelength range of the laser. After opening, the liquid 18 can be conveyed into the cavity 12b and the detection chamber 21 by centrifugal forces when the component 10 rotates about the axis 22.

For the sake of clarity, only a few cavities and structures are shown in the representation of the fluidic component. In such components, very complex fluidic systems with different functionalities can be implemented, the method according to the invention being particularly suitable for specifically opening individual cavities in order to allow a fluid to be dispensed. The fluidic component 30 according to FIG. 3 has an upper part 31a and a lower part 31b. In both parts 31a, 31b there are recesses 32a, 32b, 32c, ... and 35a, 35b, 35c, ... in the manner of the recesses of a titer plate, two recesses 32a and 35a, 32b and 35b , etc. are arranged one above the other and the two recesses are each separated by a wall 33a, 33b, .... The walls 33a, 33b, ».. are an integral part of the upper part 31a. The lower cavities 35a, 35b, 35c, ... are closed off by the walls 36a, 36b, 36c, ..., which are an integral part of the lower part 31b. A laser 39 and a mirror system 41 enable the positioning of the laser beam 40 on different areas of the fluidic component. By means of a focusing device 42, the laser beam 40 can be focused both on the walls 33a, 33b,... Or 36a, 36b,... Formed from the upper part 31a and the lower part 31b. A laser beam 40 focused on the wall 33b is shown schematically in FIG. The laser beam penetrates the lower part 31b in the area of the wall 36b, the largely transparent wall 36b not being destroyed due to the laser beam 40 being expanded in this area. After a portion of the wall 36b is destroyed, ie, a small opening is created, the liquid 43 in the cavity 32b could be dispensed into the cavity 35b. This has already been done with the cavities 32c, 35c to the right; the wall 33c therefore has an opening 38 and a liquid 44 is in the cavity 35c. The walls 33a and 36b each have an opening 34, 37 created by means of a laser.

The following results for example for this fluidic component 30

Possible application:

The recesses 32a, 32b, ... of the upper part 31a can accommodate substances for chemical conversion. After a reaction time, for example after passing through a temperature cycle, openings 39 are deliberately created in the walls 33a, 33b,... Of the upper part 31a by means of the laser 39. The substances from the upper cavities 32a, 32b, cavities 35a, 35b, ... located underneath. These cavities can already contain an active ingredient which enables the further conversion or / and analysis of the substances. The substances converted in this way can be stored in the lower cavities and, if necessary, can be released after opening the lower wall 36a, 36b, ... An analysis, for example by optical detection, of the substances in the cavities 35a, 35b, _* .. is also possible.

Reference list

Fluidic component a, 2b, 2c, ... Cavity a, 3b, 3c, ... opening wall

opening

laser

laser beam

mirror

Liquid 0 fluid component 1a, 11b upper / lower part 2a, 12b cavity 3 film 4 opening wall 5 laser 6 laser beam 7 mirror 8 liquid 0a, 20b, ... bore as feed 1 detection chamber 2 axis of rotation 0 fluid component 1a, 31b, ... upper / lower part 2a, 32b, ... cavity 3a, 33b, ... opening wall 4 opening 5a, 35b, ... opening 6a, 36b, ... opening wall 7 opening 8 opening laser

laser beam

mirror

Focusing device

liquid

Claims

claims 1. A method for dispensing a fluid located in a cavity of a fluidic component by opening at least one area of a wall delimiting the cavity, characterized by at least partially destroying the area of the wall to be opened by supplying energy by means of electromagnetic radiation, preferably laser radiation. 2. The method according to claim 1, characterized in that electromagnetic radiation of at least one wavelength is used, which is absorbed by the wall at least in the area to be opened. 3. The method according to claim 1 or 2, characterized in that electromagnetic radiation in a wavelength range from 100 nm to 12000 nm, preferably from 300 nm to 2000 nm is used. 4. The method according to any one of the preceding claims, characterized in that the electromagnetic radiation is focused on the area to be opened. 5. The method according to any one of the preceding claims, characterized in that a fluidic component with a wall delimiting the cavity, a thickness, at least in the area to be opened, less than 500 microns, preferably less than 100 microns, is used. 6. The method according to any one of the preceding claims, characterized in that a fluidic component is used with a plurality of cavities designed and arranged in the manner of the recesses of titer plates for receiving, chemical conversion, analysis or / and storage of fluids. 7. The method according to any one of the preceding claims, characterized in that the fluid is dispensed into a second cavity of the same fluidic component separated from the first cavity by the wall. 8. The method according to any one of the preceding claims, characterized in that the electromagnetic radiation is supplied such that the radiation penetrates part of the fluidic component before striking the area to be opened. 9. The method according to claim 8, characterized in that a fluidic component is used which is largely transparent to the radiation used, at least in the part to be penetrated by the radiation. 10. The method according to claim 8 or 9, characterized in that the radiation is focused on penetrating a portion of the fluidic component on the area to be opened. 11. The method according to any one of the preceding claims, characterized in that after opening the fluid is released by an applied external pressure and / or upon rotation of the fluidic component about an axis by centrifugal forces. 12. The method according to any one of the preceding claims, characterized in that the fluidic component and an electromagnetic radiation source, preferably a laser, and / or an electromagnetic beam, preferably a laser beam, are positioned relative to one another before opening. 13. Fluidic component for dispensing a fluid located in at least one cavity, the material of a wall delimiting the cavity being selected in such a way that the wall is designed such that a region of the wall for opening the cavity by supplying energy by means of electromagnetic radiation, preferably by means of Laser radiation, is destructible. 14. Fluidic component according to claim 13, characterized in that the wall has a thickness <500 microns, preferably <100 microns, at least in the area to be opened. 15. Fluidic component according to claim 13 or 14, characterized in that the wall at least in the area to be opened absorbs electromagnetic radiation in at least part of the wavelength range from 100 nm to 12000 nm, preferably from 300 nm to 2000 nm. 16. Fluidic component according to one of claims 13 to 15, characterized in that an electromagnetic radiation absorbing material is applied or integrated on or in the wall at least in the area to be opened. 17. Fluidic component according to one of claims 13 to 16, characterized in that the fluidic component is transparent at least in an area around the wall to be opened in a wavelength range in which the wall itself absorbs. 18. Fluidic component according to one of claims 13 to 17, characterized in that the fluidic component has at least one element for focusing electromagnetic radiation on the area of the wall to be opened. 19. Fluidic component according to one of claims 13 to 18, characterized in that the fluidic component consists of a one-piece polymeric part. 20. Fluidic component (1) according to one of claims 13 to 19, characterized by a plurality of cavities (2a, 2b, 2c, ...) arranged next to one another and accessible to one side. 21. Fluidic component according to claim 20, characterized in that the cavities are formed and arranged in the manner of the recesses of a titer plate. 22. Fluidic component according to one of claims 13 to 21, characterized by at least two cavities separated by a wall. 23. The fluid component (10) according to claim 22, characterized by at least two parts, an upper and a lower part (1a, 11b), the upper part (11a) and the lower part (11b) each having at least one of the cavities (12a, 12b ) and at least one film (13) separating the two cavities (12a, 12b) as a wall from each other. 24. Fluidic component (10) according to claim 23, characterized in that at least a part (11 b) of the fluidic component is transparent in a wavelength range in which the film (13) absorbs. 25. Fluidic component according to one of claims 13 to 24, characterized by more than two parts, parts having cavities and at least one film for separating cavities between parts. 26. Fluidic component according to one of claims 13 to 25, characterized in that the cavities are arranged such that a liquid in or between the cavities by applying an external pressure, by gravity or / and upon rotation of the component about an axis is movable by centrifugal forces. 27. Fluidic component according to one of claims 13 to 26, characterized in that the cavities have channels, reaction chambers, detection chambers, mixer structures, separating structures, branching structures and / or heat exchangers. 28. Device for handling fluidic components according to one of claims 13 to 27 with a receptacle for at least one such fluidic component, with at least one positioning device for the relative positioning of the fluidic component and at least one electromagnetic radiation source, preferably a laser, and / or an electromagnetic beam , preferably a laser beam, to one another and to a control unit connected to the positioning device and the electromagnetic radiation source for the targeted delivery of a fluid according to a method of claims 1 to 12. 29. The device according to claim 28, characterized by at least one focusing unit connected to the control unit for focusing the electromagnetic beam, preferably a laser beam, onto the area to be opened. 30. The device according to claim 28 or 29, characterized by a drive device for rotating the fluidic component about an axis for moving a liquid by centrifugal forces. 31. The device according to any one of claims 28 to 30, characterized by at least one reading and / or writing unit connected to the control unit for reading out or writing information stored optically and / or magnetically on the fluidic component. 32. Device according to one of claims 28 to 31, characterized by a pressure transmitter device connected to the control unit for the targeted supply of gas pressure pulses to corresponding supplies of the fluidic component.