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WO1999055841A3 - Procede d'obtention de cellules souches - Google Patents

Procede d'obtention de cellules souches Download PDF

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Publication number
WO1999055841A3
WO1999055841A3 PCT/GB1999/001333 GB9901333W WO9955841A3 WO 1999055841 A3 WO1999055841 A3 WO 1999055841A3 GB 9901333 W GB9901333 W GB 9901333W WO 9955841 A3 WO9955841 A3 WO 9955841A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
stem cells
obtaining
oocyte
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1999/001333
Other languages
English (en)
Other versions
WO1999055841A2 (fr
Inventor
Austin Smith
Peter Scott Mountford
Megan Munsie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Edinburgh
Original Assignee
University of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Edinburgh filed Critical University of Edinburgh
Priority to JP2000545985A priority Critical patent/JP2002512787A/ja
Priority to IL13932299A priority patent/IL139322A0/xx
Priority to EP99918186A priority patent/EP1073719A2/fr
Priority to AU36212/99A priority patent/AU3621299A/en
Priority to CA002329975A priority patent/CA2329975A1/fr
Publication of WO1999055841A2 publication Critical patent/WO1999055841A2/fr
Publication of WO1999055841A3 publication Critical patent/WO1999055841A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant d'obtenir une cellule souche. Ce procédé consiste à obtenir une cellule somatique contenant un gène codant pour un marqueur qui est exprimé de manière différentielle dans (a) les cellules souches requises et (b) les cellules autres que les cellules souches requises. Ce procédé consiste ensuite à obtenir un ovocyte énucléé, à transférer le noyau de la cellule somatique dans l'ovocyte énucléé pour former un ovocyte transgénique puis à cultiver ce dernier ovocyte pour produire une culture de cellules dérivées de l'ovocyte transgénique. Ce procédé consiste enfin à identifier une cellule de la culture exprimant le marqueur, ce qui indique alors que le noyau de cette cellule a été reprogrammé. L'invention traite également de dosages pour des facteurs qui peuvent reprogrammer les noyaux et les cellules souches obtenus directement ou indirectement à l'aide des procédés de reprogrammation nucléaires selon l'invention
PCT/GB1999/001333 1998-04-29 1999-04-29 Procede d'obtention de cellules souches Ceased WO1999055841A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2000545985A JP2002512787A (ja) 1998-04-29 1999-04-29 幹細胞を得る方法
IL13932299A IL139322A0 (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
EP99918186A EP1073719A2 (fr) 1998-04-29 1999-04-29 Procede d'obtention de cellules souches
AU36212/99A AU3621299A (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
CA002329975A CA2329975A1 (fr) 1998-04-29 1999-04-29 Procede d'obtention de cellules souches

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9809178.8A GB9809178D0 (en) 1998-04-29 1998-04-29 Nuclear reprogramming of somatic cells
GB9809178.8 1998-04-29

Publications (2)

Publication Number Publication Date
WO1999055841A2 WO1999055841A2 (fr) 1999-11-04
WO1999055841A3 true WO1999055841A3 (fr) 2000-01-06

Family

ID=10831203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001333 Ceased WO1999055841A2 (fr) 1998-04-29 1999-04-29 Procede d'obtention de cellules souches

Country Status (8)

Country Link
EP (1) EP1073719A2 (fr)
JP (1) JP2002512787A (fr)
AU (1) AU3621299A (fr)
CA (1) CA2329975A1 (fr)
GB (1) GB9809178D0 (fr)
IL (1) IL139322A0 (fr)
MX (1) MXPA00010473A (fr)
WO (1) WO1999055841A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11851670B2 (en) 2008-06-13 2023-12-26 Whitehead Institute For Biomedical Research Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
BR0012099A (pt) * 1999-06-30 2003-07-29 Advanced Cell Tech Inc Transferência citoplásmica para de-diferenciar células recipientes
CN1377424A (zh) * 1999-09-07 2002-10-30 先进细胞技术公司 由衰老体细胞克隆的动物中的端粒恢复和细胞寿命延长
NZ536786A (en) * 1999-09-07 2006-09-29 Advanced Cell Tech Inc Method for generating immune-compatible cells and tissues using nuclear transfer techniques
CA2394614A1 (fr) * 1999-12-20 2001-06-28 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cellules souches ou embryonnaires produites par transplantation nucleaire heterospecifique
NZ526243A (en) * 2000-11-30 2006-02-24 Stemron Inc Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
JPWO2003027277A1 (ja) * 2001-09-21 2005-01-06 独立行政法人 科学技術振興機構 再プログラム化因子のスクリーニング方法、該方法によりスクリーニングされた再プログラム化因子および該再プログラム化因子を利用する方法、並びに、未分化融合細胞を分化させる方法および細胞、組織、臓器の製造方法
CN1558949A (zh) * 2001-11-06 2004-12-29 �Ϻ��ڶ�ҽ�ƴ�ѧ 利用兔卵母细胞制备体细胞胚胎
CN1683524A (zh) * 2003-04-08 2005-10-19 东南大学 用体细胞核移植技术制备移核胚胎干细胞的方法
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
JP4901471B2 (ja) * 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
CA2683056C (fr) 2007-04-07 2020-03-24 Whitehead Institute For Biomedical Research Reprogrammation de cellules somatiques
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
CN101855350B (zh) 2008-05-02 2014-12-31 国立大学法人京都大学 核重编程序方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (fr) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection et propagation de cellules souches d'animaux transgeniques
WO1995020042A1 (fr) * 1994-01-25 1995-07-27 Ppl Therapeutics (Scotland) Limited Isolement de cellules souche embryonnaires
WO1997007669A1 (fr) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Populations de cellules quiescentes pour transfert de noyau
WO1999019469A1 (fr) * 1997-10-09 1999-04-22 Biotransplant, Inc. Cellules souches porcines comprenant un marqueur sous un promoteur oct-4

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (fr) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection et propagation de cellules souches d'animaux transgeniques
WO1995020042A1 (fr) * 1994-01-25 1995-07-27 Ppl Therapeutics (Scotland) Limited Isolement de cellules souche embryonnaires
WO1997007669A1 (fr) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Populations de cellules quiescentes pour transfert de noyau
WO1999019469A1 (fr) * 1997-10-09 1999-04-22 Biotransplant, Inc. Cellules souches porcines comprenant un marqueur sous un promoteur oct-4

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J.A. THOMSON ET AL.: "EMBRYONIC STEN CELL LUNES DERIVED FROM HUMAN BLASTOCYSTS.", SCIENCE, vol. 282, 6 November 1998 (1998-11-06), LANCASTER, PA, US, pages 1145 - 1147, XP002121340 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11851670B2 (en) 2008-06-13 2023-12-26 Whitehead Institute For Biomedical Research Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides

Also Published As

Publication number Publication date
MXPA00010473A (es) 2002-04-24
IL139322A0 (en) 2001-11-25
AU3621299A (en) 1999-11-16
GB9809178D0 (en) 1998-07-01
JP2002512787A (ja) 2002-05-08
EP1073719A2 (fr) 2001-02-07
WO1999055841A2 (fr) 1999-11-04
CA2329975A1 (fr) 1999-11-04

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