[go: up one dir, main page]

BR0012099A - Transferência citoplásmica para de-diferenciar células recipientes - Google Patents

Transferência citoplásmica para de-diferenciar células recipientes

Info

Publication number
BR0012099A
BR0012099A BR0012099-5A BR0012099A BR0012099A BR 0012099 A BR0012099 A BR 0012099A BR 0012099 A BR0012099 A BR 0012099A BR 0012099 A BR0012099 A BR 0012099A
Authority
BR
Brazil
Prior art keywords
cells
methods
differentiate
cytoplasm
cytoplasmic transfer
Prior art date
Application number
BR0012099-5A
Other languages
English (en)
Inventor
Karen B Chapman
Original Assignee
Advanced Cell Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Tech Inc filed Critical Advanced Cell Tech Inc
Publication of BR0012099A publication Critical patent/BR0012099A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

"TRANSFERêNCIA CITOPLáSMICA PARA DE-DIFERENCIAR CéLULAS RECIPIENTES". Métodos para de-diferenciação ou alteração da vida útil de células "recipientes" desejadas, por exemplo, células somáticas humanas, através de introdução de citoplasma de um tipo de célula menos diferenciado mais primitivo, por exemplo, oócito ou blastómero, são proporcionados. Esses métodos podem ser usados para produzir células do tronco embriónico e aumentar a eficiência da terapia genética permitindo que células desejadas sejam submetidas a modificações genéticas múltiplas sem se tornarem senescentes. Tal citoplasma pode ser fracionado e/ou submetido à hibridização subtrativa e os materiais ativos (suficientes para de-diferenciação) identificados e produzidos por meio de métodos recombinantes.
BR0012099-5A 1999-06-30 2000-06-30 Transferência citoplásmica para de-diferenciar células recipientes BR0012099A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14125099P 1999-06-30 1999-06-30
PCT/US2000/018063 WO2001000650A1 (en) 1999-06-30 2000-06-30 Cytoplasmic transfer to de-differentiate recipient cells

Publications (1)

Publication Number Publication Date
BR0012099A true BR0012099A (pt) 2003-07-29

Family

ID=22494867

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0012099-5A BR0012099A (pt) 1999-06-30 2000-06-30 Transferência citoplásmica para de-diferenciar células recipientes

Country Status (11)

Country Link
US (4) US20020001842A1 (pt)
EP (1) EP1196426A4 (pt)
JP (1) JP2003503071A (pt)
CN (1) CN1362965A (pt)
AU (1) AU782286B2 (pt)
BR (1) BR0012099A (pt)
CA (1) CA2377515C (pt)
IL (2) IL147179A0 (pt)
MX (1) MXPA01013450A (pt)
NZ (1) NZ516236A (pt)
WO (1) WO2001000650A1 (pt)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6800480B1 (en) 1997-10-23 2004-10-05 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
IL147179A0 (en) 1999-06-30 2002-08-14 Advanced Cell Tech Inc Cytoplasmic transfer to de-differentiate recipient cells
WO2007019398A1 (en) 2005-08-03 2007-02-15 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
MXPA02002443A (es) * 1999-09-07 2004-07-16 Advanced Cell Tech Inc Restauracion de telomeros y extension de la longevidad en animales clonados con celulas somaticas senescentes.
WO2001035735A1 (en) * 1999-11-19 2001-05-25 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US20050042749A1 (en) 2001-05-16 2005-02-24 Carpenter Melissa K. Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease
EP1287116A2 (en) 2000-05-17 2003-03-05 Geron Corporation Neural progenitor cell populations
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
NZ525607A (en) * 2000-12-22 2005-05-27 Aurox Llc Methods for cloning mammals using reprogrammed donor chromatin or donor cells
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
WO2002074935A2 (en) * 2001-03-21 2002-09-26 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals
CA2442177A1 (en) 2001-03-29 2002-10-10 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway
GB0118984D0 (en) * 2001-08-03 2001-09-26 Intercytex Ltd Fusion of cells
JP2005500847A (ja) * 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
US20030044976A1 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
WO2003054146A2 (en) 2001-11-14 2003-07-03 Northwestern University Self-assembly and mineralization of peptide-amphiphile nanofibers
US7371719B2 (en) 2002-02-15 2008-05-13 Northwestern University Self-assembly of peptide-amphiphile nanofibers under physiological conditions
AU2003254298A1 (en) 2002-08-02 2004-02-23 Stratatech Corporation Species specific dna detection
EP1391503A1 (en) * 2002-08-12 2004-02-25 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts A method of cell re-programming by cytoplasmic transfer
WO2004018628A2 (en) 2002-08-21 2004-03-04 Northwestern University Charged peptide-amphiphile solutions & self-assembled peptide nanofiber networks formed therefrom
EP1563084A4 (en) * 2002-11-08 2006-03-01 Hematech Llc TRANSGENE UNGULATES WITH REDUCED PRION PROTEIN ACTIVITY AND USES THEREOF
US7554021B2 (en) 2002-11-12 2009-06-30 Northwestern University Composition and method for self-assembly and mineralization of peptide amphiphiles
WO2004046167A2 (en) 2002-11-14 2004-06-03 Northwestern University Synthesis and self-assembly of abc triblock bola peptide
AU2004210853A1 (en) 2003-02-11 2004-08-26 Northwestern University Methods and materials for nanocrystalline surface coatings and attachment of peptide amphiphile nanofibers thereon
EP1620546A2 (en) * 2003-04-21 2006-02-01 The University of Massachusetts Reprogramming nuclear function with somatic cell cytoplasm
WO2004111195A2 (en) * 2003-06-10 2004-12-23 Trustees Of The University Of Pennsylvania Methods and use of zona-free therapeutic cloning
GB0316089D0 (en) * 2003-07-09 2003-08-13 Xo Bioscience Ltd Differentiation method
US7823789B2 (en) 2004-12-21 2010-11-02 Cognex Technology And Investment Corporation Low profile illumination for direct part mark readers
FR2861997A1 (fr) * 2003-11-06 2005-05-13 Fred Zacouto Stimulateur cardiaque orthorythmique inotrope
EP2314305A3 (en) 2003-12-05 2011-08-10 Northwestern University Self-assembling peptide amphiphiles and related methods for growth factor delivery
WO2005056576A2 (en) 2003-12-05 2005-06-23 Northwestern University Branched peptide amphiphiles, related epitope compounds and self assembled structures thereof
US20050214257A1 (en) 2003-12-23 2005-09-29 Northwestern University Compositions and methods for controlling stem cell and tumor cell differentiation, growth, and formation
CA2563064A1 (en) 2004-04-22 2005-11-10 Kirin Beer Kabushiki Kaisha Transgenic animals and uses thereof
KR20070108555A (ko) 2005-03-04 2007-11-12 노오쓰웨스턴 유니버시티 혈관신생 헤파린 결합 펩티드 양친매성 화합물
FR2885368A1 (fr) * 2005-05-04 2006-11-10 Fred Zacouto Systemes et procedes de regeneration cellulaire et utilisations de telles cellules regenerees
WO2007026255A2 (en) * 2005-06-22 2007-03-08 Universitetet I Oslo Dedifferentiated cells and methods of making and using dedifferentiated cells
US20070087437A1 (en) * 2005-10-14 2007-04-19 Jifan Hu Methods for rejuvenating cells in vitro and in vivo
US8114843B2 (en) 2005-11-18 2012-02-14 The Regents Of The University Of California Photoreactive regulator of protein function and methods of use thereof
CN101679942A (zh) 2007-02-23 2010-03-24 先进细胞技术公司 重编程分化细胞和从重编程的细胞产生动物和胚胎干细胞的高效方法
US8076295B2 (en) 2007-04-17 2011-12-13 Nanotope, Inc. Peptide amphiphiles having improved solubility and methods of using same
JP2009024027A (ja) * 2008-09-16 2009-02-05 Tadashi Goino 生理活性組成物
US9249412B2 (en) 2009-01-26 2016-02-02 Fred Zacouto Simplified method for partial genetic and epigenetic reprogramming of cells using siRNA specific for a heterochromatin protein 1
CN101798569B (zh) * 2009-02-09 2012-11-21 北京瑞途之星科技有限公司 以卵裂后的发育胚胎替代卵母细胞的治疗性克隆新方法
US8450271B2 (en) 2009-04-13 2013-05-28 Northwestern University Peptide-based scaffolds for cartilage regeneration and methods for their use
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
MX2013003681A (es) 2010-10-01 2013-11-20 Moderna Therapeutics Inc Ácidos nucleicos manipulados y métodos de uso de los mismos.
WO2012054896A1 (en) 2010-10-22 2012-04-26 Biotime Inc. Methods of modifying transcriptional regulatory networks in stem cells
DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy
CN102266350A (zh) * 2011-07-20 2011-12-07 黄必录 逆转干细胞衰老的方案
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CN102321571A (zh) * 2011-09-21 2012-01-18 王跃嗣 从分化细胞制备自体造血干细胞的方法
SI3682905T1 (sl) 2011-10-03 2022-04-29 Modernatx, Inc. Modificirani nukleozidi, nukleotidi in nukleinske kisline ter njihove uporabe
US20130156849A1 (en) 2011-12-16 2013-06-20 modeRNA Therapeutics Modified nucleoside, nucleotide, and nucleic acid compositions
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
HK1206601A1 (en) 2012-04-02 2016-01-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of biologics and proteins associated with human disease
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
SI2922554T1 (sl) 2012-11-26 2022-06-30 Modernatx, Inc. Terminalno modificirana RNA
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
CN113648323A (zh) 2013-06-05 2021-11-16 再生疗法有限公司 用于哺乳动物物种中诱导性组织再生的组合物和方法
EP3052106A4 (en) 2013-09-30 2017-07-19 ModernaTX, Inc. Polynucleotides encoding immune modulating polypeptides
SG11201602503TA (en) 2013-10-03 2016-04-28 Moderna Therapeutics Inc Polynucleotides encoding low density lipoprotein receptor
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
WO2016044721A1 (en) 2014-09-19 2016-03-24 Regenerative Medical Solutions, Inc. Compositions and methods for differentiating stem cells into cell populations comprising beta-like cells
WO2016118824A1 (en) 2015-01-22 2016-07-28 Regenerative Medical Solutions, Inc. Markers for differentiation of stem cells into differentiated cell populations
CA3188112A1 (en) 2015-03-23 2016-09-29 Astellas Institute For Regenerative Medicine Improved assays for potency of human retinal pigment epithelium (rpe) cells and photoreceptor progenitors
WO2017100313A1 (en) 2015-12-07 2017-06-15 Biotime, Inc. Methods for the re-derivation of diverse pluripotent stem cell-derive brown fat cells
CN106755113B (zh) * 2016-12-29 2020-06-19 赛业（苏州）生物科技有限公司 Es细胞基因打靶技术制备基因修饰小鼠的改进方法
JP2024534952A (ja) * 2021-09-07 2024-09-26 ユバ バイオサイエンシーズ， インコーポレイテッド 卵巣老化、卵巣疾患および状態、ならびにその症状を処置するための組成物および方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6344526A (ja) * 1986-08-12 1988-02-25 Kingo Yoshida 幼若細胞の細胞質・ミトコンドリア・イントロン他注入による若返り長寿法
US5480772A (en) 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
US5830651A (en) 1995-06-01 1998-11-03 Signal Pharmaceuticals, Inc. Human oligodendroglial progenitor cell line
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
WO1997035967A2 (en) 1996-03-28 1997-10-02 Cold Spring Harbor Laboratory Transgenic organisms with altered telomerase activity
EP0934403A4 (en) * 1996-08-19 2001-03-14 Univ Massachusetts ROWS OF EMBRYONIC OR STRAIN-LIKE CELLS PRODUCED BY CROSS NUCLEAR TRANSPLANTATION OF SPECIES
US20010012513A1 (en) 1996-08-19 2001-08-09 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
AU8587598A (en) * 1997-07-26 1999-02-16 Wisconsin Alumni Research Foundation Trans-species nuclear transfer
GB9809178D0 (en) * 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
EP1204790A4 (en) * 1999-04-23 2004-08-04 Monica Palacios-Boyce MICROELECTROMECHANICAL DEVICE FOR HANDLING CELLS OR EMBRYONS, KITS THEREFOR, METHOD FOR THE PRODUCTION THEREOF AND METHOD FOR THE USE THEREOF
US20040199935A1 (en) 1999-06-30 2004-10-07 Chapman Karen B. Cytoplasmic transfer to de-differentiate recipient cells
IL147179A0 (en) 1999-06-30 2002-08-14 Advanced Cell Tech Inc Cytoplasmic transfer to de-differentiate recipient cells
AU784731B2 (en) * 1999-12-20 2006-06-08 University Of Massachusetts Embryonic or stem-like cells produced by cross species nuclear transplantation
US20030044976A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies

Also Published As

Publication number Publication date
NZ516236A (en) 2004-12-24
CA2377515C (en) 2013-09-03
EP1196426A4 (en) 2003-09-03
US20150132853A1 (en) 2015-05-14
US20020001842A1 (en) 2002-01-03
US20130104253A1 (en) 2013-04-25
MXPA01013450A (es) 2003-09-04
CA2377515A1 (en) 2001-01-04
IL147179A0 (en) 2002-08-14
CN1362965A (zh) 2002-08-07
AU782286B2 (en) 2005-07-14
EP1196426A1 (en) 2002-04-17
IL147179A (en) 2015-01-29
WO2001000650A1 (en) 2001-01-04
AU5902800A (en) 2001-01-31
JP2003503071A (ja) 2003-01-28
US9580683B2 (en) 2017-02-28
US20170226475A1 (en) 2017-08-10

Similar Documents

Publication Publication Date Title
BR0012099A (pt) Transferência citoplásmica para de-diferenciar células recipientes
SG147468A1 (en) High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
GB2432846B (en) Medium and culture of embryonic stem cells
HUP0105052A2 (hu) Eljárások nukleinsav amplifikálására és szekvenálására
Korody et al. Rewinding extinction in the northern white rhinoceros: genetically diverse induced pluripotent stem cell bank for genetic rescue
BR0015633A (pt) Planta, polinucleotìdeo, vetor, célula, composição, polipeptìdeo, métodos para produzir uma planta e para identificar um fator que é modulado por, ou interage com, um polipeptìdeo codificado por um polinucleotìdeo, uma molécula que module a atividade ou a expressão de um polinucleotìdeo ou polipeptìdeo de interesse e uma sequência similar ou homóloga a um ou mais polinucleotìdeos, e, sistema integrado, meio computadorizado ou legìvel em computador
BR0015374A (pt) Diferenciação hematopoiética de troncocélulas embriÈnicas humanas
BR0009550A (pt) Bactéria methylophilus, métodos para produzir um l-aminoácido e para produzir células bacterianas de uma bactéria methylophilus com um teor aumentado de um l-aminoácido, e, dna
DK1681355T3 (da) Sekvensspecifik DNA-rekombination i eukaryote celler
BR9815855A (pt) Genes e métodos para controle de nematóides em plantas
WO1995034668A3 (en) The cytoplasmic inhibition of gene expression
MacGillivray et al. Transcriptional regulation in eukaryotic cells
BR9908499A (pt) Linhagens celulares embrionárias ousemelhantes a tronco produzidas portransplante nuclear de espécies cruzado
BR0211491A (pt) Geração de células dendrìticas totalmente madura e estáveis de produtos de leucafereses para aplicações clìnicas
WO2000051424A3 (en) Genetic modification of somatic cells and uses thereof
IL123411A0 (en) High efficiency methods and compositions for integrating exogenous dna into genomic dna of sperm
BR0314402A (pt) Expansão ex-vivo de populações de células raìzes em culturas celulares mononucleares
ATE386130T1 (de) Adenovirale vektoren für die gentherapie
BR0016561A (pt) Células embrionárias ou similares a células tronco produzidas por transplantes nuclear em espécie cruzadas
AR023987A1 (es) Vides resistentes a un patogeno
ES8307290A1 (es) Procedimiento para la produccion de un microorganismo que utiliza metanol geneticamente modificado.
WO2023154826A3 (en) Adaptations for high efficiency i-f3-crispr-cas systems for guide rna-directed transposition in human cells
BR9801085A (pt) Processo para a preparação de moenomicina a.
Csordás Mitochondrial transfer between eukaryotic animal cells and its physiologic role
BR0213259A (pt) Método para produzir um composto bioativo apresentando uma bioatividade conhecida, e, células recombinantes

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 8A E 9A ANUIDADES

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 2008 DE 30/06/2009.

B15K Others concerning applications: alteration of classification

Ipc: C12N 5/0735 (2010.01), A61K 48/00 (2006.01), C12N