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WO1998030992B1 - Colorants fluorescents a base de cyanine - Google Patents

Colorants fluorescents a base de cyanine

Info

Publication number
WO1998030992B1
WO1998030992B1 PCT/US1998/000475 US9800475W WO9830992B1 WO 1998030992 B1 WO1998030992 B1 WO 1998030992B1 US 9800475 W US9800475 W US 9800475W WO 9830992 B1 WO9830992 B1 WO 9830992B1
Authority
WO
WIPO (PCT)
Prior art keywords
independently
dye
integer
formula
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/000475
Other languages
English (en)
Other versions
WO1998030992A3 (fr
WO1998030992A2 (fr
Filing date
Publication date
Priority claimed from US08/781,326 external-priority patent/US6027709A/en
Priority to CA002278313A priority Critical patent/CA2278313C/fr
Priority to AU60206/98A priority patent/AU739930B2/en
Priority to AT98903434T priority patent/ATE236442T1/de
Priority to EP98903434A priority patent/EP1019887B1/fr
Priority to JP53116298A priority patent/JP4361138B2/ja
Application filed filed Critical
Priority to DE69812982T priority patent/DE69812982T2/de
Publication of WO1998030992A2 publication Critical patent/WO1998030992A2/fr
Publication of WO1998030992A3 publication Critical patent/WO1998030992A3/fr
Publication of WO1998030992B1 publication Critical patent/WO1998030992B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

Cette invention se rapporte à de nouveaux colorants fluorescents à base de cyanine qui s'avèrent utiles en tant que groupes de marquage de biomolécules.
PCT/US1998/000475 1997-01-10 1998-01-08 Colorants fluorescents a base de cyanine Ceased WO1998030992A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE69812982T DE69812982T2 (de) 1997-01-10 1998-01-08 Fluoreszierende cyaninfarbstoffe
AU60206/98A AU739930B2 (en) 1997-01-10 1998-01-08 Fluorescent cyanine dyes
AT98903434T ATE236442T1 (de) 1997-01-10 1998-01-08 Fluoreszierende cyaninfarbstoffe
EP98903434A EP1019887B1 (fr) 1997-01-10 1998-01-08 Colorants fluorescents a base de cyanine
JP53116298A JP4361138B2 (ja) 1997-01-10 1998-01-08 蛍光シアニン色素
CA002278313A CA2278313C (fr) 1997-01-10 1998-01-08 Colorants fluorescents a base de cyanine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/781,326 1997-01-10
US08/781,326 US6027709A (en) 1997-01-10 1997-01-10 Fluorescent cyanine dyes

Publications (3)

Publication Number Publication Date
WO1998030992A2 WO1998030992A2 (fr) 1998-07-16
WO1998030992A3 WO1998030992A3 (fr) 1998-11-12
WO1998030992B1 true WO1998030992B1 (fr) 1998-12-17

Family

ID=25122364

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/000475 Ceased WO1998030992A2 (fr) 1997-01-10 1998-01-08 Colorants fluorescents a base de cyanine

Country Status (8)

Country Link
US (1) US6027709A (fr)
EP (1) EP1019887B1 (fr)
JP (1) JP4361138B2 (fr)
AT (1) ATE236442T1 (fr)
AU (1) AU739930B2 (fr)
CA (1) CA2278313C (fr)
DE (1) DE69812982T2 (fr)
WO (1) WO1998030992A2 (fr)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
WO1998035012A2 (fr) * 1997-02-12 1998-08-13 Chan Eugene Y Procedes et produits permettant d'analyser des polymeres
US6114350A (en) * 1999-04-19 2000-09-05 Nen Life Science Products, Inc. Cyanine dyes and synthesis methods thereof
DE19921234B4 (de) * 1999-05-07 2005-07-28 Few Chemicals Gmbh Cyanin-Farbstoffe
WO2001066153A1 (fr) * 2000-03-03 2001-09-13 Phanos Technologies, Inc. Sondes intercalaires a membrane fluorescente et procedes d'utilisation de ces dernieres
US20030223935A1 (en) * 2001-03-05 2003-12-04 Gray Brian D. Fluorescent membrane intercalating probes and methods for their use
WO2001074950A2 (fr) * 2000-04-03 2001-10-11 Sun Chemical Corporation Pigments de mono et de bishydrazone
JP2001288197A (ja) * 2000-04-10 2001-10-16 Fuji Photo Film Co Ltd 蛍光ヌクレオチド
DE10018199A1 (de) 2000-04-12 2001-10-31 Few Chemicals Gmbh Fluoreszenzmarker
CN101525660A (zh) * 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
US6397150B1 (en) 2000-07-27 2002-05-28 Visible Genetics Inc. Method and apparatus for sequencing of DNA using an internal calibrant
DE10046215B4 (de) * 2000-09-19 2004-04-15 Institut für Chemo- und Biosensorik Münster e.V. i.Ins. Fluorochrome und deren Verwendung
JP4989013B2 (ja) * 2000-09-19 2012-08-01 リ−コール インコーポレーティッド シアニン色素
US7597878B2 (en) * 2000-09-19 2009-10-06 Li-Cor, Inc. Optical fluorescent imaging
WO2002026891A1 (fr) 2000-09-29 2002-04-04 Molecular Probes, Inc. Colorants carbocyanines modifies et leurs conjugues
CA2425663C (fr) * 2000-10-11 2009-12-29 Applera Corporation Conjugues fluorescents a nucleobase contenant des lieurs anioniques
US6663847B1 (en) * 2000-10-13 2003-12-16 Mallinckrodt Inc. Dynamic organ function monitoring agents
AU2002236683A1 (en) * 2000-10-27 2002-05-21 Beth Israel Deaconess Medical Center Non-isotopic detection of osteoblastic activity in vivo using modified bisphosphonates
US6331632B1 (en) * 2000-11-09 2001-12-18 Beckman Coulter, Inc. Cyanine dye phosphoramidites
EP1354064A2 (fr) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres
EP1221465A1 (fr) * 2001-01-03 2002-07-10 Innosense S.r.l. Colorants polyméthiniques symétriques et monofonctionnalisés comme réactifs de marquage
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
JP4676654B2 (ja) * 2001-07-19 2011-04-27 富士フイルム株式会社 蛍光ヌクレオチド及びそれを用いた標識法
US6533396B2 (en) 2001-08-16 2003-03-18 Benq Corporation Printhead cartridge with asymmetrical contacts
US20050032060A1 (en) * 2001-08-31 2005-02-10 Shishir Shah Arrays comprising pre-labeled biological molecules and methods for making and using these arrays
EP1451318A4 (fr) 2001-10-12 2007-06-27 Perkinelmer Las Inc Compilations d'acides nucleiques, reseaux et procedes d'utilisation de ceux-ci
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
US6838289B2 (en) * 2001-11-14 2005-01-04 Beckman Coulter, Inc. Analyte detection system
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
AU2003225763A1 (en) * 2002-03-11 2003-09-29 Visen Medical, Inc. Optical imaging probes
US6916621B2 (en) * 2002-03-27 2005-07-12 Spectral Genomics, Inc. Methods for array-based comparitive binding assays
JP2005532434A (ja) * 2002-07-01 2005-10-27 グアヴァ テクノロジーズ，インコーポレーテッド 蛍光色素、エネルギー転移ペア及び方法
US8188116B2 (en) * 2002-09-04 2012-05-29 Vanderbilt University Agents for therapy efficacy monitoring and deep tissue imaging
US7754884B2 (en) * 2005-01-03 2010-07-13 Vanderbilt University Targeted, NIR imaging agents for therapy efficacy monitoring, deep tissue disease demarcation and deep tissue imaging
US7005518B2 (en) * 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
US20040132092A1 (en) * 2003-01-03 2004-07-08 Stetson Christopher M. Determining the density of functional moieties on polymer reagents
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
ATE550099T1 (de) 2003-04-04 2012-04-15 Hoffmann La Roche Verbessertes system für mehrfarbige echtzeit-pcr
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (fr) * 2003-07-29 2005-01-29 Invitrogen Corporation Tests de kinase et de phosphatase
US7619059B2 (en) * 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
US7271265B2 (en) * 2003-08-11 2007-09-18 Invitrogen Corporation Cyanine compounds and their application as quenching compounds
DE602004025592D1 (de) * 2003-09-12 2010-04-01 Life Technologies Corp Anwendungen des Resonanzenergietransfers zwischen Terbium und GFP
AU2004286845A1 (en) * 2003-10-30 2005-05-19 Tufts-New England Medical Center Prenatal diagnosis using cell-free fetal DNA in amniotic fluid
US7776529B2 (en) * 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
WO2005056687A2 (fr) 2003-12-05 2005-06-23 Molecular Probes, Inc. Colorants de cyanine a substitution de methine
US7446202B2 (en) * 2003-12-05 2008-11-04 Molecular Probes, Inc. Cyanine dye compounds
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
US7230117B2 (en) * 2004-07-12 2007-06-12 Beckman Coulter, Inc. Stable cyanine dye phosphoramidites
EP1885718B1 (fr) 2005-05-11 2017-03-15 Life Technologies Corporation Composes chimiques fluorescents possedant une selectivite elevee pour l'adn double brin, et procedes d'utilisation
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
FR2889700B1 (fr) * 2005-08-11 2012-11-23 Synthinnove Lab Marqueurs, leur procede de fabrication et leurs applications
JP5106397B2 (ja) * 2005-09-02 2012-12-26 ビセン メディカル， インコーポレイテッド 生体適合性ｎ，ｎ−二置換スルホンアミド含有蛍光色素標識
WO2007028163A1 (fr) * 2005-09-02 2007-03-08 Visen Medical, Inc. Agents d'imagerie fluorescentes biocompatibles
EP1934202B1 (fr) 2005-09-02 2019-01-09 Visen Medical, Inc. Fluorophores proches infrarouge derives d'acide nicotinique et d'acide picolinique
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US8802025B2 (en) * 2007-09-27 2014-08-12 Sysmex Corporation Reagent kit for sample analysis and sample analysis method
US20090214436A1 (en) * 2008-02-18 2009-08-27 Washington University Dichromic fluorescent compounds
CA2723315A1 (fr) 2008-04-30 2009-11-05 Ben-Gurion University Of The Negev Research And Development Authority Systemes d'administration vasculaire
JP5500875B2 (ja) * 2009-01-30 2014-05-21 キヤノン株式会社 新規化合物、該新規化合物を用いたプローブ及び該新規化合物もしくは該プローブを用いた蛍光イメージング用造影剤
US9155471B2 (en) 2009-05-27 2015-10-13 Lumicell, Inc'. Methods and systems for spatially identifying abnormal cells
WO2010138738A1 (fr) 2009-05-27 2010-12-02 Lumicell Diagnostics, Inc. Procédés et systèmes destinés à identifier de manière spatiale des cellules anormales
WO2011151814A2 (fr) 2010-05-29 2011-12-08 Ben-Gurion University Of The Negev Research And Development Authority Conjugués peptide-polymère bloqués de pénétration cellulaire pour diagnostic et applications thérapeutiques
WO2012054749A1 (fr) 2010-10-20 2012-04-26 Li-Cor, Inc. Colorants à base de cyanine et leurs conjugués
US9314304B2 (en) 2010-12-08 2016-04-19 Lumicell, Inc. Methods and system for image guided cell ablation with microscopic resolution
EP3338617B1 (fr) 2012-01-23 2020-08-19 Washington University Systèmes et dispositifs d'imagerie par lunette
US10791937B2 (en) 2013-03-14 2020-10-06 Lumicell, Inc. Medical imaging device and methods of use
CA2901000C (fr) * 2013-03-15 2023-03-14 Visen Medical, Inc. Colorants d'heptamethine cyanine pontee par cyclohexyle 4,4-disubstitue et leurs utilisations
AR102743A1 (es) 2014-11-26 2017-03-22 Li Cor Inc Medios de contraste ir para imágenes por fluorescencia
WO2016179350A1 (fr) 2015-05-06 2016-11-10 Washington University Composés à motifs de ciblage rd et leurs procédés d'utilisation
US10300153B2 (en) 2015-11-30 2019-05-28 Asthra, Llc Immunological detection of altered cells
CN106280533B (zh) * 2016-08-17 2018-03-09 赣南师范大学 一种近红外荧光染料及其合成方法和用于寄生虫荧光标记
EP3609543A1 (fr) 2017-04-11 2020-02-19 Li-Cor, Inc. Colorants cyanine solides
WO2019213543A1 (fr) 2018-05-04 2019-11-07 Li-Cor, Inc. Colorants d'hémicyanine de 2-[2-[4-[bis(2-sulfoéthyl)amino]phényl]éthényl]-1-butyl-3,3-diméthyl-3h-indolium pour la détection d'anticorps et d'autres biomolécules
US20220025432A1 (en) * 2018-12-06 2022-01-27 Futuresynthesis Spólka Z O.O. Method for fluorescence labeling of biological materials, thermally removable fluorescent labels for this method, and methods of their preparation and use
WO2021119423A1 (fr) 2019-12-13 2021-06-17 Washington University Colorants fluorescents dans l'infrarouge proche, formulations et procédés associés
EP4423502A1 (fr) 2021-10-26 2024-09-04 Caribou Biosciences, Inc. Analyse de l'activité d'une endonucléase en temps réel couplée à l'exonucléase

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5091519A (en) * 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
CA1340806C (fr) * 1986-07-02 1999-11-02 James Merrill Prober Methode, systeme et reactifs pour le sequencage de l'adn
US5047519A (en) * 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5151507A (en) * 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
DE3912046B4 (de) * 1988-09-02 2004-03-25 Carnegie Mellon University Verfahren zum Markieren einer Komponente einer wäßrigen Flüssigkeit und lumineszenzphotostabiles Reaktionsprodukt
JPH07145148A (ja) * 1992-06-02 1995-06-06 Bio Sensor Kenkyusho:Kk ポリメチン系化合物およびそれを用いる測定方法
US5366623A (en) * 1992-09-11 1994-11-22 Colonel Clair Apparatus for magnetically treating a fluid
JP3176163B2 (ja) * 1993-01-28 2001-06-11 イビデン株式会社 蛍光標識試薬および蛍光免疫測定法
EP0620703B1 (fr) * 1993-04-12 1997-12-29 Ibiden Co, Ltd. Compositions à base de résine et plaques à circuit imprimé utilisant celles-ci
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
DE4326466A1 (de) * 1993-08-06 1995-02-09 Boehringer Mannheim Gmbh Infrarot-Farbstoff-markierte Nucleotide und ihre Verwendung in der Nucleinsäure-Detektion
JP3399598B2 (ja) * 1993-09-17 2003-04-21 イビデン株式会社 Ｎ−部分置換アミノグリカンを用いた免疫測定用標識試薬
JPH0783925A (ja) * 1993-09-17 1995-03-31 Ibiden Co Ltd 蛍光標識試薬および蛍光免疫測定法
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
CN1093768C (zh) * 1995-01-30 2002-11-06 第一化学药品株式会社 诊断用标记物

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