[go: up one dir, main page]

WO1998019794A1 - Procede et dispositif pour la fabrication d'une plaque a cupules, notamment pour des echantillons de produits chimiques ou biologiques - Google Patents

Procede et dispositif pour la fabrication d'une plaque a cupules, notamment pour des echantillons de produits chimiques ou biologiques Download PDF

Info

Publication number
WO1998019794A1
WO1998019794A1 PCT/US1997/019280 US9719280W WO9819794A1 WO 1998019794 A1 WO1998019794 A1 WO 1998019794A1 US 9719280 W US9719280 W US 9719280W WO 9819794 A1 WO9819794 A1 WO 9819794A1
Authority
WO
WIPO (PCT)
Prior art keywords
sheet
wells
mold
plate
holes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/019280
Other languages
English (en)
Inventor
Thierry Dannoux
Gilbert Pujol
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corning Inc
Original Assignee
Corning Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9613530A external-priority patent/FR2755380B1/fr
Application filed by Corning Inc filed Critical Corning Inc
Priority to EP97911923A priority Critical patent/EP0936944A1/fr
Priority to JP52148298A priority patent/JP2001511078A/ja
Publication of WO1998019794A1 publication Critical patent/WO1998019794A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C51/00—Shaping by thermoforming, i.e. shaping sheets or sheet like preforms after heating, e.g. shaping sheets in matched moulds or by deep-drawing; Apparatus therefor
    • B29C51/18—Thermoforming apparatus
    • B29C51/20—Thermoforming apparatus having movable moulds or mould parts
    • B29C51/22—Thermoforming apparatus having movable moulds or mould parts rotatable about an axis
    • B29C51/225—Thermoforming apparatus having movable moulds or mould parts rotatable about an axis mounted on a vacuum drum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C2791/00—Shaping characteristics in general
    • B29C2791/004—Shaping under special conditions
    • B29C2791/006—Using vacuum

Definitions

  • the present invention relates to a method and a device for the manufacture of a plate of wells, notably for samples of chemical or biological products and, more particularly, to such a plate made from a sheet made of a thermoformed material in the plastic state.
  • Such plates of wells are known to carry out biological tests or cultures, and they are made from a sheet of organic plastic material (for example, polycarbonate) which has been thermoformed.
  • organic plastic material for example, polycarbonate
  • the samples contained in the wells often have to be observed with a microscope through the bottom of the wells, notably when these samples contain living cells which collect at the bottom of these wells as a result of Plates made of organic plastic material are generally not suitable for such observation because of unfavorable optical properties such as birefringence, fluorescence of the material, surfaces without optical finish, etc.
  • microtitration plate which is made of injection-molded plastic material, this plate comprising a two-dimensional distribution of bottomless recesses.
  • a plate of polycarbonate of optical quality is glued against all the recesses so as to form the bottoms of what become individual wells.
  • Gluing plates together also negatively impacts the cost of the manufacture of the plate.
  • the purpose of the present invention is to make a plate of wells which presents none of these drawbacks and which, consequently, permits observations with a microscope, while at the same time being inexpensive to manufacture .
  • This purpose of the invention is achieved, as well as others which will become apparent upon reading the following description, by a method for the manufacture of a plate of wells of the type described in the preamble of the present description.
  • the method comprises the steps of: a) providing a mold, which is perforated by a plurality of holes arranged according to a distribution of wells to be formed; b) placing a sheet of thermoformable material on said mold and heating said sheet to a temperature where it is in the plastic state, and c) establishing a gas pressure between the two sides of the sheet, so that the material of said sheet, being perpendicular to the holes of the mold, is aspired into these holes thereby assuming the shape of wells whereby the bottoms of the wells are separated frcm any contact with other surfaces of the mold.
  • this method allows the two opposite surfaces of the bottom of a well to preserve an optical quality surface or finish state which is compatible with observation through this bottom, this optical finish remaining intact because during the molding, any contact between the bottom of the well and other surfaces capable of altering its surface state is avoided.
  • the invention provides a device comprising a) a mold having a side capable of receiving a sheet of the thermoformable material, said mold being perforated, perpendicularly to this side, by a plurality of holes, b) an enclosure delimiting with said mold a space, c) means to heat the sheet and to bring it to the plastic state, and d) a low- pressure gas generator which can selectively be connected to said space to thermoform wells in said sheet, by aspiration into said holes of the material of said sheet located perpendicular to said holes.
  • the method according to the invention allows the making of a plate of wells comprising a distribution of thermoformed wells in a sheet made of a thermoplastic material, which plate is characterized in that two sides of the wall of the wells formed in the sheet present an optical finish.
  • the thermoplastic material used is preferably an inorganic glass.
  • Figure 1 is a diagram of a first embodiment of a device for the implementation of the method according to the invention.
  • Figure 2 is a perspective view of a part of a plate of wells obtained using the device of Figure 1.
  • Figure 3 is a cross-sectional view of the plate according to the invention, mounted on a frame adapted to this plate.
  • Figure 4 is a diagram of a second embodiment of a device to implement the method according to the invention. DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • FIG. 1 of the drawing in the appendix where a cross-sectional representation is given of an enclosure 1 delimiting a space 2 which can be placed in communication with a low-pressure generator (not shown) through the intermediary of a pipe 3.
  • the enclosure is closed by a mold 4 which assumes the general shape of a planar plate perforated by cylindrical holes 5 l f 5 2 ,..., 5 , etc., having axes perpendicular to its surface.
  • These holes can also be conical and in that cast they have a conicity of 5-20°. They can have a circular or polygonal section, for example, square, rounded at the edges.
  • the holes preferably have identical geometry and they are arranged in a dense regular two-dimensional distribution, in lines and in columns.
  • the enclosure 1 is placed in an oven, after having placed a sheet of thermoplastic material such as an inorganic glass or an organic material such as a polycarbonate on the mold 4.
  • the surface state of the sheet must be of compatible optical quality with that which is desired for the walls of the bottoms of the wells to be made.
  • the oven is heated at a temperature where the glass reaches a viscosity compatible with a thermoforming operation.
  • a low pressure is established in the spact 2, compared to the gas pressure existing in the oven, and notably at the level of the surface of the plate opposite that which faces the space 2. Due to the gas pressure difference, the glass plate is deformed in the areas of this plate covering the holes 5 ⁇ . These areas are aspired towards the space 2 and they then gradually assume an approximately hemispherical shape.
  • the temperature of the plate, the duration of application of the low pressure in the enclosure 1 and the value of this low pressure are adjusted so that during the course of their progressive deformation, the aspired areas of the plat e remain constantly separated from the surfaces of the hole and from any other surface in general.
  • the manufacture of a plate 6 of wells l l r 7 2 .. • , l ⁇ , etc. is ensured, as represented in cross section in Figure 1, the two sides of the wall of each well presenting a perfect optical finish because of the absence of any contact between these surfaces and the surfaces of the mold during the operation of forming these wells by thermoforming under a vacuum.
  • the optical finish so obtained is very advantageous in the observation of biological cultures, for example, through the bottoms of the wells where these cultures are carried out.
  • the material used to make the plate is a material of optical quality, s 1 . h as an organic or inorganic glass.
  • the selection of a mineral glass is particularly appropriate, because of its chemical inertness.
  • the plate of well 6 according to the invention presents wells whose wall is thinner at the bottom of the wells than at their opening because of the vacuum thermoforming method used to make these wells.
  • the method of manufacture according to the invention thus allows the making of plates with wells having a small bottom thickness, less than 0.2 mm, for example, compatible with observations using a microscope with a very short focal length, such as, for example, confocal microscopes.
  • a microscope with a very short focal length such as, for example, confocal microscopes.
  • the lens 20 of such a microscope is shown in the position of observation of a bottom of a well, through a drop 21 :f an index- adapting liquid.
  • the plate is made from a planar glass sheet referenced 0211 in the catalogues of the company Corning Incorporated, this plate covering a surface of 80 mm x 116 mm and comprising 96, 384, or 1536 wells arranged in 8, 16, or 32 adjacent rows of 12, 24, or 48 wells, respectively.
  • the plate can also have the standard dimensions of a microscope slide (26 x 76 mm) and then comprise, for example, 60 or 250 wells.
  • the separation of the wells is compatible with the usual means for multichannel distribution with pipettes.
  • a mold 4 is made of a refractory alloy referenced NS 30 in the catalogues of the company UGINE (AFNOR Z12CN25-20), this mold being perforated by holes having a depth of 2 mm and a diameter of 2 mm, these holes being separated by walls having a thickness of at least 0.25 mm and advantageously presenting a clearance angle of 5-20°C [sic] .
  • Such holes can be obtained by mechanical or chemical machining.
  • an alcohol suspension of powdered calcium hydroxide is prepared, the latter having a granular size of less than 1 mm, and a dispersant is added, such as the agent DISPERBYK 190 from the company BYK Chemie (Germany) .
  • This suspension facilitates the separation of the plate of wells and the mold, after the thermoforming of this plate. Being applied against the top surface of the mold, this suspension does not affect the surface state of the bottom of the wells.
  • planar glass sheet is mounted on the mold 4 and the plate/mold/enclosure assembly is placed in an oven, the latter is heated to a temperature of approximately 675-695°C, and this temperature is maintained for approximately 5-7 min, while applying a 0.75-bar low pressure to the space 2.
  • the starting planar glass sheet preferably has a thickness of 0.3 mm.
  • the bottoms of the wells present, as seen above, a smaller thickness, in this instance approximately 0.160 mm.
  • Such a thickness is compatible with the focal length of the lenses of microscopes, notably confocal microscopes, which routinely are 0.2 mm.
  • Figure 2 represents a fraction of the surface of the plate obtained, as one can visualize it using a microscope with mechanical scanning.
  • the generally hemispherical shape of the well l ⁇ r 7 1+ ⁇ , etc. appears in this figure, which also shows that the openings of the wells are connected to each other by surfaces with a progressive curvature, without discontinuities.
  • This geometry is advantageous from at least two points of - r iew. It prevents air bubbles from being trapped on the surrace when cultures in a liquid medium are distributed in the wells. It also promotes the collection of live cells at the bottom of the wells as a result of gravity, as will be seen below.
  • Two methods can be used to distribute cultures in a liquid medium or, more generally, any liquid, in the different wells.
  • the first consists in using standard micropipettes, separated or in sets.
  • the second method consists in depositing on the plate a drop which simultaneously covers several adjacent wells. By gravity, this liquid spreads over the plate possibly filling other adjacent wells. If the liquids contain plant or animal cells, one can note that they distribute themselves as a result of gravity between the wells and they advantageously regroup at their bottoms.
  • a hydrophobic product such as a silane, for example.
  • a treatment of this surface contributes to the filling of the wells by preventing any liquid from remaining between them. It also avoids any communication of liquid between two adjacent wells after the filling of these wells.
  • This treatment permits the handling, without particular precaution, of a plate of 250 or 1536 wells having a diameter of 2 mm, for example, and such a plate can even be turned upside down for hanging-drop cultures, for example.
  • the plate 6 of wells according to the invention may be supported by means of an emptied frame 8 presenting an edge 8' against which the periphery of the plate 6 is rested, as shown in Figure 3.
  • the plate 6 can possibly be glued or welded to the frame 8, at the level of the edge 8'.
  • the frame 8 has a height which is greater than the thickness of the plate 6 so as to protect the bottom of the wells from any contact with a surface on which the frame is placed. The latter also facilitates the manual or automatic handling operations of the plate according to the invention.
  • FIG. 4 is a representation of another embodiment of the device for the implementation of the method according to the invention, which is adapted to continuous production, and is also advantageous from an economical point of view.
  • the device comprises a reservoir (not shown) of a molten glass which discharges a sheet of glass 19 having an adjusted viscosity through a slit 9.
  • This sheet is taken up by a cylindrical drum 10 which is mounted so it can rotate around the fixed shaft 11 and so it is driven by means (not shown) at a predetermined speed.
  • the drum 10 is integrally connected to a cylindrical core 12 which is internal to the drum.
  • the surface of this core is slightly separated from the internal surface of the drum 10, resting against axial ribs such as 13 ⁇ , 13 2 , etc., formed, for example, on the core 12.
  • the fixed shaft 11 is hollowed out in the form of peripheral chamber 14 which communicates, through the intermediary of a radial channel 15 and an axial channel 16 with a low-pressure generator (not shown) .
  • the chamber 14 communicates at each instant with one of several radial channels, such as 17 ⁇ , 17 2 , 17 3/ etc., by one of the annular chambers such as 18 ⁇ , 18 2 , etc., present between the drum 10 and the core 12, at the level of the ribs 13 x .
  • the drum 10 plays the role of a mold for the sheet of glass 19 which envelopes this drum.
  • the drum is perforated by holes 5',., just like the mold 4 of Figure 1.
  • the low-pressure generator being connected to the pipe 16, the sheet 19 of glass which continually descends from the slit 9 is grabbed by aspiration by the surface of the drum, this sheet then enve. ⁇ ping this drum over approximately one-fourth of its surface.
  • the sheet 19 is subjected to a low pressure at the level of its side which is in contact with this drum, thanks to the pipe 17 x which transmits, at a given instant, a low pressure to the corresponding rotatory annular chamber 18 ⁇ , 18 2 .
  • the holes 5 perforated into the drum 10 then play the role of the holes 5 X of the mold of Figure 1, in the formation of wells in the sheet of glass 19, by separating the surface of these wells from any contact during their formation, as seen above in connection with Figure 1.
  • the flow rates of glass exiting the hopper 9, the speed of rotation of the drum, the temperature and the thickness of the sheet 19 as well as the low pressure applied to this sheet are adjusted so that essentially hemispherical wells are obtained, similar to those represented in Figure 1.
  • the sheet so deformed leaves the drum 10 to be then cut at a high temperature and then cooled by means which are not shown, to form section 6 1 6 Xi ;, etc., which are each capable of constituting a plate of wells according to the invention.
  • the device of Figure 4 ensures a continuous production of plates, which production is better adapted than the device of Figure 1 to large production volumes.
  • the temperature of the glass can be in the range of 740-760°C, and the contact time between the glass sheet and the drum on the order of only 5-20 sec.
  • the temperature of the drum is maintained, by means which are not shown, at approximately 550-600°C.
  • the invention in ed allows achieving the set purpose, that is to ensure the manufacture at reduced cost of plates of wells which allow the observation with a microscope of the content of the wells through their bottoms.
  • the plate also presents the advantage of potentially containing a variable number of wells, which can be very high, the manufacturing method described being suitable for small-volume wells, for example, 0.2-10 itiL. With a very large number of wells, it is possible to simultaneously treat a great number of samples, for example, 100,000 samples/day, as is done routinely today.
  • the wells of the plate accor ing to the invention because of their geometry and their discontinuity-free connections lend themselves to the formation of a convex meniscus of the contained liquid in each well.
  • the liquid contains live cells, they thus benefit from a maximum surface for exchanging oxygen with the air, which is favorable to their development.
  • the contamination of the samples contained in the wells by toxins borne by the internal surface of these wells, and also the dissipation of any solvents contained in the wells through the walls, are particularly important if the well has a small volume.
  • the use according to the invention of glass with high chemical inertness to make plates of such wells with small volume is then particularly advantageous to minimize this problem.
  • the present invention allows the making of plates of wells with essentially hemispherical bottoms.
  • this form advantageously allows a reduction of the volumes of reagent coating the beads.
  • the plate according to the invention can be used to carry out tests on samples of chemical products and not only biological products or materials.
  • inorganic glass is preferred to make this plate because of its above-mentioned chemical inertia, other mineral or organic thermoplastic materials, for example, could be used.
  • other mater als can be used if no observation through the bottom of the well is to be carried out.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Blow-Moulding Or Thermoforming Of Plastics Or The Like (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

Dans le procédé de l'invention, une feuille constituée d'un matériau à l'état plastique est traitée par thermoformage. On fabrique un moule (4) qui présente plusieurs perforations (5i) réparties selon un motifs de répartition de cupules à former dans ladite feuille. On place ladite feuille sur ledit moule (4) et on la porte à une température à laquelle elle est à l'état plastique, et on crée une différence de pression de gaz entre les deux côtés de la feuille de sorte que le matériau de cette dernière, qui est perpendiculaire aux orifices du moule, soit aspiré dans ces derniers et prenne la forme des cupules (7i) dont le fond est isolé de tout contact avec d'autres surfaces.
PCT/US1997/019280 1996-11-06 1997-10-23 Procede et dispositif pour la fabrication d'une plaque a cupules, notamment pour des echantillons de produits chimiques ou biologiques Ceased WO1998019794A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP97911923A EP0936944A1 (fr) 1996-11-06 1997-10-23 Procede et dispositif pour la fabrication d'une plaque a cupules, notamment pour des echantillons de produits chimiques ou biologiques
JP52148298A JP2001511078A (ja) 1996-11-06 1997-10-23 特に化学的または生物学的生成物の試料用のウェルを備えたプレートを製造する方法および装置

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR9613530A FR2755380B1 (fr) 1996-11-06 1996-11-06 Procede et dispositif de fabrication d'une plaque de puits, notamment pour echantillons de produits chimiques ou biologiques
FR96/13530 1996-11-06
US4491397P 1997-04-25 1997-04-25
US60/044,913 1997-04-25

Publications (1)

Publication Number Publication Date
WO1998019794A1 true WO1998019794A1 (fr) 1998-05-14

Family

ID=26233088

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/019280 Ceased WO1998019794A1 (fr) 1996-11-06 1997-10-23 Procede et dispositif pour la fabrication d'une plaque a cupules, notamment pour des echantillons de produits chimiques ou biologiques

Country Status (4)

Country Link
EP (1) EP0936944A1 (fr)
JP (1) JP2001511078A (fr)
CN (1) CN1232412A (fr)
WO (1) WO1998019794A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999061152A1 (fr) * 1998-05-26 1999-12-02 Mj Research, Inc. Cartouche de prelevement en forme de microplaquette pouvant etre automatisee
WO2008064430A1 (fr) * 2006-12-01 2008-06-05 Swinburne University Of Technology Moulage d'empreinte de microstructures pour soutenir un matériau biologique microscopique
CN103030262A (zh) * 2011-09-30 2013-04-10 富泰华工业（深圳）有限公司 曲面玻璃板的成型方法与其采用的成型设备
US10308542B2 (en) 2015-06-26 2019-06-04 Corning Incorporated Apparatus and method for reforming sheet material
US20220304298A1 (en) * 2019-06-19 2022-09-29 Advanced Institute Of Reproductive Technologies Instrument for use in operating cell
WO2023154736A1 (fr) * 2022-02-11 2023-08-17 Joon Bu Park Adhésifs tissulaires composites à coefficient de poisson positif et négatif

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7718133B2 (en) * 2003-10-09 2010-05-18 3M Innovative Properties Company Multilayer processing devices and methods
CN104619826A (zh) * 2012-09-14 2015-05-13 住友电木株式会社 微孔板
WO2014047520A1 (fr) * 2012-09-20 2014-03-27 Danisco Us Inc. Plaques de microtitration pour une libération contrôlée d'éléments de culture vers des cultures cellulaires
EP2719670B1 (fr) * 2012-10-12 2019-08-14 Corning Incorporated Procédés pour former du verre elliptique et des ébauches de miroir à coque sphérique
EP2786856B1 (fr) * 2013-04-04 2016-05-18 Magna Steyr Fuel Systems GesmbH Dispositif et procédé de fabrication de récipients en plastique
JP6185816B2 (ja) * 2013-10-11 2017-08-23 Ａｇｃテクノグラス株式会社 細胞培養容器

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3518725A (en) * 1967-09-13 1970-07-07 Craft Master Corp Machine for forming multiple cavity containers from thermoplastic sheet material
DE1704211A1 (de) * 1967-12-21 1971-04-22 Geng & Co Gmbh Kg Maschf Verfahren und Vorrichtung zum Herstellen von Kunststoffolien mit ueber die ganze Breite in Abstaenden verteilten Eindrueckungen bzw. Erhebungen
US5041266A (en) * 1989-12-21 1991-08-20 Hoffmann-La Roche Inc. Tray for immunometric determinations
WO1992001513A1 (fr) * 1990-07-18 1992-02-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Plaque comportant au moins un puits destine a recevoir des substances chimiques et/ou biochimiques et/ou microbiologiques et procede de fabrication de la plaque
DE4217868A1 (de) * 1992-05-29 1993-12-02 Univ Schiller Jena Temperierbare Multiküvette
DE4424112A1 (de) * 1994-07-08 1996-01-11 Raytest Isotopenmesgeraete Gmb Verfahren zur Herstellung eines Probenträgers

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3518725A (en) * 1967-09-13 1970-07-07 Craft Master Corp Machine for forming multiple cavity containers from thermoplastic sheet material
DE1704211A1 (de) * 1967-12-21 1971-04-22 Geng & Co Gmbh Kg Maschf Verfahren und Vorrichtung zum Herstellen von Kunststoffolien mit ueber die ganze Breite in Abstaenden verteilten Eindrueckungen bzw. Erhebungen
US5041266A (en) * 1989-12-21 1991-08-20 Hoffmann-La Roche Inc. Tray for immunometric determinations
WO1992001513A1 (fr) * 1990-07-18 1992-02-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Plaque comportant au moins un puits destine a recevoir des substances chimiques et/ou biochimiques et/ou microbiologiques et procede de fabrication de la plaque
DE4217868A1 (de) * 1992-05-29 1993-12-02 Univ Schiller Jena Temperierbare Multiküvette
DE4424112A1 (de) * 1994-07-08 1996-01-11 Raytest Isotopenmesgeraete Gmb Verfahren zur Herstellung eines Probenträgers

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999061152A1 (fr) * 1998-05-26 1999-12-02 Mj Research, Inc. Cartouche de prelevement en forme de microplaquette pouvant etre automatisee
WO2008064430A1 (fr) * 2006-12-01 2008-06-05 Swinburne University Of Technology Moulage d'empreinte de microstructures pour soutenir un matériau biologique microscopique
CN103030262A (zh) * 2011-09-30 2013-04-10 富泰华工业（深圳）有限公司 曲面玻璃板的成型方法与其采用的成型设备
US10308542B2 (en) 2015-06-26 2019-06-04 Corning Incorporated Apparatus and method for reforming sheet material
US20220304298A1 (en) * 2019-06-19 2022-09-29 Advanced Institute Of Reproductive Technologies Instrument for use in operating cell
US12342811B2 (en) * 2019-06-19 2025-07-01 Advanced Institute Of Reproductive Technologies Instrument for use in operating cell
WO2023154736A1 (fr) * 2022-02-11 2023-08-17 Joon Bu Park Adhésifs tissulaires composites à coefficient de poisson positif et négatif
US12350396B2 (en) 2022-02-11 2025-07-08 Joon Bu Park Composite positive and negative Poisson's ratio tissue adhesives

Also Published As

Publication number Publication date
JP2001511078A (ja) 2001-08-07
EP0936944A1 (fr) 1999-08-25
CN1232412A (zh) 1999-10-20

Similar Documents

Publication Publication Date Title
WO1998019794A1 (fr) Procede et dispositif pour la fabrication d'une plaque a cupules, notamment pour des echantillons de produits chimiques ou biologiques
US6764653B2 (en) Method of forming well(s) containing filtration devices
US5096676A (en) Crystal growing apparatus
US7092154B2 (en) Apparatus for microscopic observation of long-term culture of single cell
EP2342317B1 (fr) Plaque à gouttes suspendues
CN105682802B (zh) 一种微流控装置及控制其流体流动的方法
JP5231271B2 (ja) 側面通気用の蓋を含む培養プレート
EP2408900B1 (fr) Appareil pour la culture de micro-organismes et procédé d'utilisation de cet appareil
WO1986007606A1 (fr) Plaque d'essais a cavites multiples
CN102481571A (zh) 生物微流体芯片及相关方法
WO1996021851A2 (fr) Dispositif d'analyse biologique a caracteristique de prevention de contamination amelioree
EP1851304A1 (fr) Dispositif de culture
CN116547376A (zh) 用于细胞培养的微流体细胞培养装置和方法
US20030219360A1 (en) One piece filtration plate
AU2005272546B2 (en) Micro channel array
JP2009542208A (ja) ハイスループット・スクリーニングのための高密度透過性支持体
US20050051471A1 (en) Underdrain useful in the construction of a filtration device
CN114390948B (zh) 微流控芯片、生产方法及用途
US8470110B2 (en) Sample chamber and method for the production thereof
EP1815910A2 (fr) Récipients ou pointes jetables incluant des régions ultraminces, et leurs procédés de fabrication
WO2001074991A1 (fr) Plaque d'expansion de culture cellulaire
CN120115206B (zh) 一种无源离散型浓度梯度芯片及无源离散型浓度梯度的建立方法
US7404873B2 (en) Membrane attachment process
WO2025172168A1 (fr) Dispositif fluidique d'examen d'échantillons biologiques et ensemble dispositif fluidique
JP2007526451A (ja) プラットフォームに沿う交差汚染を防止するのに使用される装置、およびその装置を製造する方法

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 97198551.0

Country of ref document: CN

AK Designated states

Kind code of ref document: A1

Designated state(s): CN JP US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase

Ref document number: 1998 521482

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 09297785

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 1997911923

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1997911923

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1997911923

Country of ref document: EP