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WO1996033214A3 - Altered protein expression in hypoxic trophoblasts - Google Patents

Altered protein expression in hypoxic trophoblasts Download PDF

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Publication number
WO1996033214A3
WO1996033214A3 PCT/US1996/005441 US9605441W WO9633214A3 WO 1996033214 A3 WO1996033214 A3 WO 1996033214A3 US 9605441 W US9605441 W US 9605441W WO 9633214 A3 WO9633214 A3 WO 9633214A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
hypoxic
trophoblasts
cell types
protein expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/005441
Other languages
French (fr)
Other versions
WO1996033214A2 (en
Inventor
Susan J Fisher
Olga Genbacev
Russell Foulk
Karl R Clauser
Alama L Burlingame
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Priority to EP96912916A priority Critical patent/EP0822940A2/en
Priority to AU55575/96A priority patent/AU718493B2/en
Priority to JP8531343A priority patent/JPH11504318A/en
Publication of WO1996033214A2 publication Critical patent/WO1996033214A2/en
Publication of WO1996033214A3 publication Critical patent/WO1996033214A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4715—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This invention is the discovery that hypoxic mammalian fetal trophoblast cells are a valid model of abnormal maternal plancental function and of other invasive cell types. The trophoblast cells differentially express a number of proteins when grown under hypoxic conditions. These proteins are useful for determining the health of the trophoblasts, the condition of a maternal placental interface and the invasive properties of metastatic and other invasive cell types. The hypoxic cytotrophoblast cells provide a convenient means to isolate these proteins and develope assay kits based upon the presence or absence of the proteins in a human test sample.
PCT/US1996/005441 1995-04-18 1996-04-18 Altered protein expression in hypoxic trophoblasts Ceased WO1996033214A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP96912916A EP0822940A2 (en) 1995-04-18 1996-04-18 Altered protein expression in hypoxic trophoblasts
AU55575/96A AU718493B2 (en) 1995-04-18 1996-04-18 Altered protein expression in hypoxic trophoblasts
JP8531343A JPH11504318A (en) 1995-04-18 1996-04-18 Changes in protein expression in hypoxic trophoblasts

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42340995A 1995-04-18 1995-04-18
US08/423,409 1995-04-18

Publications (2)

Publication Number Publication Date
WO1996033214A2 WO1996033214A2 (en) 1996-10-24
WO1996033214A3 true WO1996033214A3 (en) 1996-12-27

Family

ID=23678805

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/005441 Ceased WO1996033214A2 (en) 1995-04-18 1996-04-18 Altered protein expression in hypoxic trophoblasts

Country Status (5)

Country Link
EP (1) EP0822940A2 (en)
JP (1) JPH11504318A (en)
AU (1) AU718493B2 (en)
CA (1) CA2218535A1 (en)
WO (1) WO1996033214A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1208381A2 (en) * 1999-08-13 2002-05-29 Oxford GlycoSciences (UK) Limited Proteins, genes and their use for diagnosis and treatment of breast cancer

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU827030A1 (en) * 1978-12-27 1981-05-07 Московский Ордена Трудового Красногознамени Медицинский Стоматологическийинститут Им. H.A.Семашко Method of diagnosis of fetus condition
US4943527A (en) * 1985-10-04 1990-07-24 California Biotechnology Inc. Mature apoai protein production under serum free culturing conditions
US5141849A (en) * 1990-06-08 1992-08-25 The United States Of America As Represented By The Department Of Health And Human Services Marker for early detection of human hydatidiform moles and choriocarcinomas
US5196523A (en) * 1985-01-01 1993-03-23 The University Of Southern California Control of gene expression by glucose, calcium and temperature
WO1993024838A1 (en) * 1992-05-29 1993-12-09 Adeza Biomedical Corporation Screening method for identifying women at increased risk for preterm delivery

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU827030A1 (en) * 1978-12-27 1981-05-07 Московский Ордена Трудового Красногознамени Медицинский Стоматологическийинститут Им. H.A.Семашко Method of diagnosis of fetus condition
US5196523A (en) * 1985-01-01 1993-03-23 The University Of Southern California Control of gene expression by glucose, calcium and temperature
US4943527A (en) * 1985-10-04 1990-07-24 California Biotechnology Inc. Mature apoai protein production under serum free culturing conditions
US5141849A (en) * 1990-06-08 1992-08-25 The United States Of America As Represented By The Department Of Health And Human Services Marker for early detection of human hydatidiform moles and choriocarcinomas
WO1993024838A1 (en) * 1992-05-29 1993-12-09 Adeza Biomedical Corporation Screening method for identifying women at increased risk for preterm delivery

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 8207, Derwent World Patents Index; Class B04, AN 82-13463E, XP002011721 *
GENBACEV O ET AL.: "Hypoxia alters early gestation human cytotrophoblast differentiation/invasion in vitro and models the placental defects that occur in preeclampsia", J. CLIN. INVEST, vol. 97, no. 2, 15 January 1996 (1996-01-15), NEW YORK, NY; US, pages 540 - 550, XP000579303 *

Also Published As

Publication number Publication date
JPH11504318A (en) 1999-04-20
CA2218535A1 (en) 1996-10-24
AU5557596A (en) 1996-11-07
EP0822940A2 (en) 1998-02-11
AU718493B2 (en) 2000-04-13
WO1996033214A2 (en) 1996-10-24

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