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WO1988005819A1 - ADN DE PLASMIDES RECOMBINANT pPR-IFNbeta1-13, CODANT POUR LA SYNTHESE D'INTERFERON-beta1 DE FIBROBLASTE HUMAIN, PROCEDE POUR SA CONSTRUCTION ET SOUCHE DE BACTERIES ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) A TITRE DE PRODUCTEUR D'INTERFERON-beta1 HUMAIN, LE CONTENANT - Google Patents

ADN DE PLASMIDES RECOMBINANT pPR-IFNbeta1-13, CODANT POUR LA SYNTHESE D'INTERFERON-beta1 DE FIBROBLASTE HUMAIN, PROCEDE POUR SA CONSTRUCTION ET SOUCHE DE BACTERIES ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) A TITRE DE PRODUCTEUR D'INTERFERON-beta1 HUMAIN, LE CONTENANT Download PDF

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Publication number
WO1988005819A1
WO1988005819A1 PCT/SU1988/000033 SU8800033W WO8805819A1 WO 1988005819 A1 WO1988005819 A1 WO 1988005819A1 SU 8800033 W SU8800033 W SU 8800033W WO 8805819 A1 WO8805819 A1 WO 8805819A1
Authority
WO
WIPO (PCT)
Prior art keywords
gene
dηκ
ppr
interferon
ρρκ
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/SU1988/000033
Other languages
English (en)
Russian (ru)
Inventor
Vladimir Georgievich Debabov
Jury Ivanovich Kozlov
Sergei Vladimirovich Mashko
Alexandr Yakovlevich Strongin
Viktor Emilievich Sterkin
Vitaly Lvovich Jurin
Marina Ivanovna Lebedeva
Maxim Eduardovich Trukhan
Sergei Mikhailovich Podkovyrov
Alla Lvovna Lapidus
Andrei Vladimirovich Mochulsky
Lara Semenovna Izotova
Anna Stanislavovna Ryzhavskaya
Andrei Petrovich Alexenko
Sergei Viktorovich Kostrov
Marina Alexeevna Skvortsova
Valdemaras Vitautovich Gervinskas
Elena Alexeevna Nosovskaya
Ljudmila Vladimirovna Evdonina
Vitaly Arkadievich Livshits
Vladas-Algirdas Vladovich Bumyalis
Sergei Ionovich Borukhov
Tatyana Grigorievna Plotnikova
Alexandr Petrovich Bolotin
Eugenijus-Arvidas Andreevich Yanulaitis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VSESOJUZNY NAUCHNO-ISSLEDOVATELSKY INSTITUT GENETI
Original Assignee
VSESOJUZNY NAUCHNO-ISSLEDOVATELSKY INSTITUT GENETI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VSESOJUZNY NAUCHNO-ISSLEDOVATELSKY INSTITUT GENETI filed Critical VSESOJUZNY NAUCHNO-ISSLEDOVATELSKY INSTITUT GENETI
Priority to NL8820103A priority Critical patent/NL8820103A/nl
Priority to HU881692A priority patent/HUT50871A/hu
Priority to DE19883890050 priority patent/DE3890050T1/de
Publication of WO1988005819A1 publication Critical patent/WO1988005819A1/fr
Priority to FI884556A priority patent/FI884556A7/fi
Priority to GB8823728A priority patent/GB2208866A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta

Definitions

  • the area of technology is not limited to the field of genetic engineering and biotechnology, and more specifically.
  • the synthesizer used in this case provides an intricate mixture of its various products and functions, which is a result of a complicated operation. This essentially complicates the following properties of individual interactions. ⁇ connection with it more and more
  • the appliance receives the input of individual components from the microprocessor-friendly synthesis.
  • the invention is also a method of combining a p-combination plasmid for D- ⁇ ⁇ 13 ⁇ -13, which is also true for ⁇ ⁇ 24 north,
  • the indicated, the wiring is also the headquarters of the Bacterium ⁇ Igenetica ⁇ 903 ( ⁇ - ⁇
  • the strain is claimed to ensure that the person is safe to eat and / or to eat and / or drink;
  • the method of eliminating the declared industrial requirement for the maintenance of ⁇ > 1-13 is not limited to several stages.
  • the ⁇ - ⁇ zh 1> 1-123 means that the proximity of the ST-sequential gene is closed for the second / second gene
  • the transformer will receive cells ⁇ . ⁇ ⁇ ⁇ 600, which eliminates the stress on the medium with an amperage with a direct voltage and a voltage of 28 ° ⁇ .
  • the battery is suitable for a temperature range of 5 to 40 ° C (optimum 35 ° C) for 6.7-7.5.
  • ⁇ ⁇ aches ⁇ ve is ⁇ chnzh ⁇ a ugle ⁇ da and ⁇ lzuyu ⁇ amin ⁇ zhsl ⁇ y w uglev ⁇ dy (na ⁇ ime ⁇ , sa ⁇ a ⁇ zu) 20 Is ⁇ chni ⁇ m az ⁇ a m ⁇ gu ⁇ sluzhi ⁇ mzhne ⁇ alnye s ⁇ li in nzhyn ⁇ y ⁇ me atm and ⁇ a ⁇ zhe ⁇ ganzhches ⁇ zhe s ⁇ edzhenzhya in vzhde ⁇ e ⁇ na, ⁇ zh ⁇ na, d ⁇ zhzhev ⁇ g ⁇ e ⁇ s ⁇ a ⁇ a w amin ⁇ isl ⁇ Us ⁇ ychzhv ⁇ s ⁇ ⁇ an ⁇ zhbzh ⁇ zh ⁇ am.
  • the strain is stable to concentrate up to 100 mg / l and shruzhzhvanzhi 25 burned on agazirovannyh nutritional
  • Plasma stability P ⁇ zh ⁇ anenzhzh ⁇ le ⁇ on ar ⁇ iz ⁇ vann ⁇ y s ⁇ ede (s ⁇ m I d ⁇ months) ⁇ i se ⁇ zhzh ⁇ sl d ⁇ va ⁇ elny ⁇ ⁇ e ⁇ esev ⁇ v (in ⁇ echenie at least 6 months) in ⁇ tsesse glubzhnn ⁇ g ⁇ ⁇ ul ⁇ ivzh ⁇ vanzhya in zhzhd ⁇ y s ⁇ ede 30 an ⁇ zhbi ⁇ zh ⁇ m not ⁇ zhs ⁇ di ⁇ ⁇ e ⁇ i Well ⁇ e ⁇ es ⁇ y ⁇ zh ⁇ lazm dy.
  • Phase I In fact, even less than the last plasmid is 12 V.
  • 2 mcg of the plasmid ⁇ 24 24 enlarges the local distribution of excrement in 40 flop of the isolation buffer ⁇ .
  • the accessory 3--parts of the participants of the Département I ⁇ . s ⁇ in ⁇ be ⁇ bem ⁇ m m ⁇ l 20, 10 s ⁇ de boiling m ⁇ ⁇ is- ⁇ S ⁇ , ⁇ 8,0, 10 m ⁇ m ⁇ 2, 2 m ⁇ g ⁇ e ⁇ a D ⁇ , ⁇ 30 m ⁇ dez ⁇ si ⁇ zhb ⁇ nu ⁇ le ⁇ zzhd ⁇ zh ⁇ s ⁇ a ⁇ v 5 edzh ⁇ e ⁇ men ⁇ a.
  • Two of the reactive mixtures transfer ethane and dissolve in 100 ⁇ l ⁇ ⁇ ⁇ .
  • the resulting mixture is the translucent bore gasket of the C600 C600.
  • the accessory of the transformer was composed up to 5 » 10 at the same level as the first one at a time ⁇ 24. It obstructs clones that are stable for amplification (100 mcg / ml), but they also emit a separate module for modifying
  • the received message also contains a fragment ( ⁇ ⁇ mulegi ⁇ ) that burns into the receiver ⁇ 24 ⁇ .
  • the obtained product is in the range of 150 ⁇ l, containing 50 mt of trap-n ⁇ terrorism, ⁇ 8.0, 10 mk ⁇ of ethylenejaminetetus, as a result
  • the remote end participants have been remotely operated by an end user of 100 ⁇ l, which is 2 at a time.
  • the nucleotide installed by the method of Sengar, the investigation of the hybrid participation of the communal food in the structure of the device, is the following:
  • Shamazmidu ⁇ ⁇ - ⁇ / 31-13 which supports the synthesis of a human being, has an analogous connection to the bathroom, which is included in the manual
  • 35 ⁇ 903 is around 10 slots on the 1st week of August - 16 - ⁇ ⁇ whatsoever ⁇ Operations ⁇ - ⁇ - ⁇ . Particles are removed from a medium containing amperage (100 ⁇ g / ml) after a fresh bulb is removed at a temperature of 28 ° C. From all the oblique, they isolate plasmid DB
  • the facility is equipped with a fully equipped facility.
  • the medium is available at 100 ⁇ g / ml and 10 g / l glucose.
  • the seed crop is introduced in the range of 5-10 ma.
  • the 25th is the level of supply of ammonia water. Part Pe ⁇ vuyu ⁇ e ⁇ men ⁇ atszhzh ⁇ v ⁇ dya ⁇ ⁇ zh ⁇ em ⁇ e ⁇ a ⁇ u ⁇ e 28 ° C d ⁇ ⁇ zhches ⁇ ⁇ l ⁇ n ⁇ s ⁇ zh, ⁇ avn ⁇ y 3.5 ⁇ i 550 nm and za ⁇ em ⁇ susches ⁇ vlyayu ⁇ ⁇ e ⁇ d ⁇ zhndu ⁇ zhyu, ⁇ vyshaya ⁇ em ⁇ e ⁇ a ⁇ u ⁇ u d ⁇ 42-45 ° C in a 5 ⁇ echenzh mzhn, ⁇ sle cheg ⁇ ⁇ d ⁇ lzhayu ⁇ ⁇ e ⁇ len ⁇ atszhyu still chasa 2 ⁇
  • Zayavlyaemyzh sh ⁇ amm ba ⁇ e ⁇ y ⁇ zs ⁇ eg ⁇ s ⁇ and s ⁇ ⁇ IIgene ⁇ i ⁇ a ⁇ ⁇ 903 ( ⁇ - ⁇ Y ⁇ - ⁇ z> - ⁇ dutsen ⁇ /> 1-iya ⁇ e ⁇ e ⁇ on chel ⁇ ve ⁇ a na ⁇ di ⁇ ⁇ zhmenenie in mi ⁇ bi ⁇ l ⁇ giches ⁇ y and meditsins ⁇ y ⁇ myshleyan ⁇ s ⁇ i ⁇ lucheniya for 1 h 15 in ⁇ e ⁇ e ⁇ na l ⁇ ve ⁇ a, ⁇ y m ⁇ zhe ⁇ ⁇ imenya ⁇ sya in meditsins ⁇ y ⁇ a ⁇ i ⁇ e .

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un ADN de plasmide recombinant pPR-IFNbeta1-13, codant pour la synthèse d'interféron-beta1 humain, présente une dimension de 3,9 k.b.p. et est constitué par les éléments suivants: BamHI-BglII, un fragment d'une taille de 3,4 k.b.p. de vecteur de plasmide obtenu sur la base de pPR40 et pML24, EcoRI-Sau3A, un fragment de plasmide pINbeta-trp 7 d'une taille de 510 b.p. deposé le 12.01.87 sous le No. 1825 à la collection de cultures de l'Institut de Recherches Soviétique pour les antibiotiques. Un procédé de construction de l'ADN de plasmides précité comprend la construction préliminaire du vecteur de plasmide pPR124B, puis la construction du plasmide intermédiaire pPR-IFNbeta1-123 suivie de la construction du plasmide pPR-IFNbeta1-13. Une souche de bactéries Escherichia coli VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) à titre de producteur d'interféron-beta1 humain a été déposée le 12.01.87 sous le No. 1825 à la collection de cultures de l'Institut de Recherches Soviétique pour les antibiotiques.
PCT/SU1988/000033 1987-02-09 1988-02-08 ADN DE PLASMIDES RECOMBINANT pPR-IFNbeta1-13, CODANT POUR LA SYNTHESE D'INTERFERON-beta1 DE FIBROBLASTE HUMAIN, PROCEDE POUR SA CONSTRUCTION ET SOUCHE DE BACTERIES ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) A TITRE DE PRODUCTEUR D'INTERFERON-beta1 HUMAIN, LE CONTENANT Ceased WO1988005819A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
NL8820103A NL8820103A (nl) 1987-02-09 1988-02-08 Recombinant plasmide dna - phr-ifnbeta 1-13 coderende synthese van fibroblastisch humaan beta1-interferon, werkwijze ter bereiding ervan en een stam van de bacterie escherichia coli vniigenetika vl 903 (phr-ifnbeta1-13), die humaan beta1-interferon vormt, die dit bevat.
HU881692A HUT50871A (en) 1987-02-09 1988-02-08 Process for producing recombinant plasmid dna encoding human fibroblast-beta 1-interferon and escherichia coli comprising same
DE19883890050 DE3890050T1 (de) 1987-02-09 1988-02-08 Rekombination-plasmid-dns ppr-ifn(beta)1-13 die die synthese des menschlichen fibroblast-(beta)1-interferons kodiert, verfahren zum konstruieren derselben und stamm der bakterien
FI884556A FI884556A7 (fi) 1987-02-09 1988-10-04 Rekombinantplasmid-dna ppr-ifn 1-13, som kodas foer syntes av maenniskans fibroblast- 1-interferon, foerfarande foer framstaellning daerav och e.coli innehaollande detta.
GB8823728A GB2208866A (en) 1987-02-09 1988-10-10 Recombinant plasmid dna ppr-ifn¼-13, coding for synthesis of human fidroblast ¼-1 interferon, and strain of bacteria escherichia coli vniigenetika vl903

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SU4191345/28 1987-02-09
SU874191345A SU1703692A1 (ru) 1987-02-09 1987-02-09 Рекомбинантна плазмидна ДНК @ -1 @ 1-13, кодирующа синтез фибробластного интерферона ( @ I) человека, способ ее конструировани , штамм бактерий ЕSснеRIснIа coLI - продуцент @ I-интерферона человека

Publications (1)

Publication Number Publication Date
WO1988005819A1 true WO1988005819A1 (fr) 1988-08-11

Family

ID=21284355

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SU1988/000033 Ceased WO1988005819A1 (fr) 1987-02-09 1988-02-08 ADN DE PLASMIDES RECOMBINANT pPR-IFNbeta1-13, CODANT POUR LA SYNTHESE D'INTERFERON-beta1 DE FIBROBLASTE HUMAIN, PROCEDE POUR SA CONSTRUCTION ET SOUCHE DE BACTERIES ESCHERICHIA COLI VNIIGENETIKA VL 903 (pPR-IFNbeta1-13) A TITRE DE PRODUCTEUR D'INTERFERON-beta1 HUMAIN, LE CONTENANT

Country Status (9)

Country Link
JP (1) JPH01502478A (fr)
CH (1) CH676996A5 (fr)
FI (1) FI884556A7 (fr)
GB (1) GB2208866A (fr)
HU (1) HUT50871A (fr)
NL (1) NL8820103A (fr)
SE (1) SE8803567L (fr)
SU (1) SU1703692A1 (fr)
WO (1) WO1988005819A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0593792B2 (fr) * 1992-10-14 2004-01-07 Ajinomoto Co., Inc. Nouvelle micro-bactérie capable de produire de la L-thréonine et procédé de production de la L-thréonine
NZ555979A (en) * 2004-12-20 2009-04-30 Cadila Healthcare Ltd Process for preparing high levels of interferon beta
RU2386694C2 (ru) * 2006-05-05 2010-04-20 ООО "Биотех" Штамм дрожжей pichia pastoris - продуцент гамма-интерферона цыпленка, рекомбинантная плазмидная днк, обеспечивающая биосинтез гамма-интерферона цыпленка, и способ ее конструирования

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"Biotekhnologia", pod, red. A.A. BAEVE, 1984, Nauka, (Moscow), pages 204-205. *
ZHURNAL VSESOJUZNOGO KHIMICHESKOGO OBSCHESTVA IM. D. I. MENDELCEVA, tom XXIX Nr. 2, 1984, (Khimia, Moscow), E.D. SVERDLOV, "Interferony i Geneticheskaya Inzheneria", pages 86-89, (206-209). *

Also Published As

Publication number Publication date
CH676996A5 (fr) 1991-03-28
FI884556A0 (fi) 1988-10-04
GB2208866A (en) 1989-04-19
SE8803567D0 (sv) 1988-10-07
GB8823728D0 (en) 1988-12-07
SU1703692A1 (ru) 1992-01-07
HUT50871A (en) 1990-03-28
JPH01502478A (ja) 1989-08-31
NL8820103A (nl) 1989-01-02
FI884556A7 (fi) 1988-10-04
SE8803567L (sv) 1988-10-07

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