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US20150038445A1 - Methods and compositions for treating pain - Google Patents

Methods and compositions for treating pain Download PDF

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US20150038445A1
US20150038445A1 US14/378,013 US201314378013A US2015038445A1 US 20150038445 A1 US20150038445 A1 US 20150038445A1 US 201314378013 A US201314378013 A US 201314378013A US 2015038445 A1 US2015038445 A1 US 2015038445A1
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pain
substituted
unsubstituted
acid
compound
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Chih-Cheng Chen
Yun-Lian Lin
Jim-Min Fang
Yijuang Chern
Chia-Ching John Lin
Wei-Nan Chen
Chun-Jung Lin
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Academia Sinica
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Assigned to ACADEMIA SINICA reassignment ACADEMIA SINICA ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: LIN, CHUN-JUNG
Assigned to ACADEMIA SINICA reassignment ACADEMIA SINICA ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: LIANG, CHI-MING
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Assigned to ACADEMIA SINICA reassignment ACADEMIA SINICA ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHEN, Wei-nan, CHERN, YIJUANG, FANG, JIM-MIN, LIN, YUN-LIAN, CHEN, CHIH-CHENG, LIN, CHIA-CHING JOHN
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A61K36/8988—Gastrodia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A61P29/02—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] without antiinflammatory effect

Definitions

  • the present invention is directed to medical methods and compositions for treatment of pain, in particularly acid-induced pain.
  • Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage, for example, which may result from damage to body tissue, for example in general medical illnesses, cancer, neuropathies, and perioperative conditions. Pain may also be associated with medical disorders without a known cause, such as migraine and psychosomatic illness.
  • Substance P is an undecapeptide belonging to the tachykinin small peptide family. SP is a pain neurotransmitter that helps excite and transmit pain signals from neural cells in many organs. Substance P is an important element in pain perception. The sensory function of substance P is thought to be related to the transmission of pain information into the central nervous system. Substance P coexists with the excitatory neurotransmitter glutamate in primary afferents that respond to painful stimulation. (De Felipe et al., Altered nociception, analgesia and aggression in mice lacking the receptor for substance P.
  • the SP signaling could alter action potential thresholds and modulate the expression of TTX-resistant sodium currents in medium-sized muscle mociceptor. (Lin et al., An antinociceptive role for substabce P in acid-induced chronic muscle pain. PNAS 109 (2): E76-E83, January 2012.)
  • the present invention is based on the unexpected discovery that a number of adenosine analogs are effective for the treatment of pain through activation of neurokinin 1 (NK1) receptor signaling pathway in muscle nociceptors, thereby inducing the activation of activation of M-type potassium channel to induce outward currents.
  • NK1 neurokinin 1
  • the present invention provides a use in the manufacture of a medicament for treating pain of an adenonsine analog that activates NK1 receptor signaling, thereby inducing outward current.
  • the pain is an acid-induced pain.
  • the pain is an acid-induced muscle pain, particularly an acid-induced chronic muscle pain.
  • the pain may also be selected from the group consisting of inflammatory pain, cancer pain, chest pain, back pain, facial pain, joint pain, muscular pain syndromes, neuropathic pain, peripheral pain, cancer and tumor pain, sympathetic pain, postoperative pain, and post-traumatic pain.
  • the pain may also be fibromyalgia, myofascial pain, bladder pain syndrome or pain casued by irritable bowel syndrome.
  • the adenosine analog is isolated from a Gastrodia extract.
  • the adenosine analog is a compound of Formula (I):
  • X is 0, S or CH 2 ;
  • R 1 is selected from the group consisting of NHR 4 , NH(CH 2 ) n R 4 , NH—NHR 4 , NHCONHR 4 , NH—OR 4 , O—NHR 4 , and SR 4 ;
  • R 2 is selected from the group consisting of hydrogen (H), halogen, cyano, OR 4 , NHR 4 , substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl (Ar), substituted or unsubstituted aralkyl, and a substituted or unsubstituted heterocyclyl;
  • R 3 is selected from the group consisting of halomethyl, hydroxymethyl (HOCH 2 ), alkoxymethyl (R 4 OCH 2 ), azidomethyl (N 3 CH 2 ), aminomethyl (H 2 NCH 2 ), substituted or unsubstituted aminomethyl, amidomethyl (H 2 NCOCH 2 ), sulfanylmethyl (R 4 S), sulfonylmethyl (R 4 SO 2 ), triazolylmethyl, cyanomethyl (N ⁇ CCH 2 ), cyano, substituted or unsubstituted carbonyl (R 4 CO), COOH, substituted or unsubstituted aminocarbonyl (R 4 HNCO), substituted or unsubstituted alkynyl, and substituted or unsubstituted tetrazole;
  • n 1, 2 or 3;
  • each instance of R 4 is independently selected from the group consisting of H, alkyl, cycloalkyl, substituted or unsubstituted Ar, substituted or unsubstituted aralkyl, and a substituted or unsubstituted heterocyclyl;
  • Ar is selected from the group consisting of substituted or unsubstituted phenyl, substituted or unsubstituted polyarene, and a substituted or unsubstituted heterocycle.
  • the adenosine analog is a compound of Formula (II):
  • the adenosine analog is N 6 -(4-hydroxybenzyl)adenosine having the formula T1-11:
  • the adenosine analog is a compound having the formula JMF1998:
  • the adenosine analog is a compound having the formula JMF2665:
  • the adenosine analog is a compound of formula (III):
  • Het is a substituted or unsubstituted heterocycle comprising 5- or 6-membered ring or fused ring containing at least one nitrogen, oxygen or sulfur heteroatoms.
  • Het is selected from the group consisting of pyrrole, furan, thiophene, pyridine, piperidine, piperazine, indole, benzofuran, benzothiophene, and quinoline.
  • the adenosine analog is a compound having the formula JMF1907:
  • the adenosine analog is a compound of formula (IV):
  • the adenosine analog is a compound having the formula CGS21680:
  • the adenosine analog according to the present disclosure can be selected from the following:
  • the present invention provides a pharmaceutical composition of use for treating pain (e.g., those described herein) comprising an effective amount of an andenosine analog as defined above, and a pharmaceutically acceptable carrier.
  • the present invention provides a method of treating pain, comprising administering to a subject in need thereof an effective amount of the andenosine analog as described herein, or a pharmaceutical composition comprising the andenosine analog as defined above.
  • the term “subject” refers to a human or a mammal, such as a patient, a companion animal (e.g., dog, cat, and the like), a farm animal (e.g., cow, sheep, pig, horse, and the like) or a laboratory animal (e.g., rat, mouse, guinea pig, and the like).
  • a companion animal e.g., dog, cat, and the like
  • a farm animal e.g., cow, sheep, pig, horse, and the like
  • a laboratory animal e.g., rat, mouse, guinea pig, and the like.
  • treatment refers to obtaining a desired pharmacologic and/or physiologic effect.
  • the effect may be prophylactic in terms of completely or partially preventing a disease or condition, or symptom thereof, and/or may be therapeutic in terms of a partial or complete cure for a condition and/or adverse affect attributable to the condition.
  • treatment covers any treatment of a disease or condition in a mammal, particularly in a human, and includes: (a) preventing the condition from occurring in a subject which may be predisposed to the condition but has not yet been diagnosed as having it; (b) inhibiting the development of the condition; and/or (c) relieving the condition, i.e., causing its regression.
  • an effective amount refers to an amount sufficient to effect beneficial or desired results.
  • An effective amount can be administered in one or more administrations.
  • An effective amount corresponds with the quantity required to provide a desired average local concentration of a particular biologic agent, in accordance with its known efficacy, for the intended period of therapy.
  • a dose may be determined by those skilled in the art by conducting preliminary animal studies and generating a dose response curve, as is known in the art. Maximum concentration in the dose response curve would be determined by the solubility of the compound in the solution and by toxicity to the animal model, as known in the art.
  • the effective amount further corresponds with the quantity required to provide a desired average local concentration of the particular biologic agent, in accordance with its efficacy for the intended period of time. Due allowance can be made for losses due to circulatory fluctuation due to physical activity, for example, from ten to ninety percent loss allowance could be made depending upon the individual patient and their routines.
  • pain refers to an unpleasant feeling often caused by intense or damaging stimuli, including different types and symptoms of pain, either acute or chronic pain, especially inflammatory pain, cancer pain, chest pain, back pain, facial pain, joint pain, muscular pain syndromes, neuropathic pain, peripheral pain, cancer and tumor pain, sympathetic pain, postoperative pain, and post-traumatic pain.
  • alkyl is given its ordinary meaning in the art and refers to the radical of saturated aliphatic groups, including straight-chain alkyl groups, branched-chain alkyl groups, cycloalkyl (alicyclic) groups, alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups.
  • the alkyl group may be a lower alkyl group, i.e., an alkyl group having 1 to 10 carbon atoms (e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, or decyl).
  • a straight chain or branched chain alkyl may have 30 or fewer carbon atoms in its backbone, and, in some cases, 20 or fewer. In some embodiments, a straight chain or branched chain alkyl may have 12 or fewer carbon atoms in its backbone (e.g., C 1 -C 12 for straight chain, C 3 -C 12 for branched chain), 6 or fewer, or 4 or fewer. Likewise, cycloalkyls may have from 3-10 carbon atoms in their ring structure, or 5, 6 or 7 carbons in the ring structure.
  • alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, cyclopropyl, butyl, isobutyl, t-butyl, cyclobutyl, hexyl, and cyclochexyl.
  • alkenyl and alkynyl are given their ordinary meaning in the art and refer to unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but that contain at least one double or triple bond respectively.
  • cycloalkyl refers specifically to groups having three to ten, preferably three to seven carbon atoms. Suitable cycloalkyls include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and the like, which, as in the case of other aliphatic, heteroaliphatic, or hetercyclic moieties, may optionally be substituted with substituents including, but not limited to aliphatic; heteroaliphatic; aryl; heteroaryl; arylalkyl; heteroarylalkyl; alkoxy; aryloxy; heteroalkoxy; heteroaryloxy; alkylthio; arylthio; heteroalkylthio; heteroarylthio; —F; —Cl; —Br; —I; —OH; —NO 2 ; —CN; —CF 3 ; —CH 2 CF
  • aryl or “Ar” as used herein is given its ordinary meaning in the art and refers to aromatic carbocyclic groups, substituted or unsubstituted, having a single ring (e.g., phenyl), or multiple fused rings, including phenyl, polyarene, and heterocycle comprising 5- or 6-membered ring or fused ring containing at least one nitrogen, oxygen or sulfur heteroatoms.
  • Substituents include, but are not limited to the substituents recited for aliphatic moieties, or for other moieties as disclosed herein, resulting in the formation of a stable compound.
  • the heterocycle may be 3- to 10-membered ring structures or 3- to 7-membered rings, whose ring structures include one to four heteroatoms.
  • Heterocycles include, for example, thiophene, benzothiophene, thianthrene, furan, tetrahydrofuran, pyran, isobenzofuran, chromene, xanthene, phenoxathiin, pyrrole, dihydropyrrole, pyrrolidine, imidazole, pyrazole, pyrazine, isothiazole, isoxazole, pyridine, pyrazine, pyrimidine, pyridazine, indolizine, isoindole, indole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, triazole, tetrazole, oxazole, isoxazole, thiazole, isothiazole
  • the heterocyclic ring can be optionally substituted at one or more positions with such substituents as described herein.
  • the heterocycle may be bonded to a compound via a heteroatom ring atom (e.g., nitrogen).
  • the heterocycle may be bonded to a compound via a carbon ring atom.
  • the heterocycle is pyridine, imidazole, pyrazine, pyrimidine, pyridazine, acridine, acridin-9-amine, bipyridine, naphthyridine, quinoline, benzoquinoline, benzoisoquinoline, phenanthridine-1,9-diamine, or the like.
  • pharmaceutically acceptable salt refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
  • Pharmaceutically acceptable salts are well known in the art. For example, Berge et al., describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated by reference herein for the purposes or subject matter referenced herein.
  • Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases.
  • Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
  • organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate,
  • Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N + (C 1-4 alkyl) 4 salts.
  • Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like.
  • Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counter ions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
  • the compound is in the form of a hydrate or solvate.
  • hydrate refers to a compound non-covalently associated with one or more molecules of water.
  • solvate refers to a compound non-covalently associated with one or more molecules of an organic solvent.
  • prodrugs of the compounds described herein for treating pain is also within the scop of the present disclosure.
  • prodrugs include esters and other pharmaceutically acceptable derivatives, which, upon administration to a subject, are capable of providing the indole compounds described above (see Goodman and Gilman's, The Pharmacological basis of Therapeutics, 8th ed., McGraw-Hill, Int. Ed. 1992, “Biotransformation of Drugs”).
  • FIG. 1 shows the effects of the compound T1-11 in inhibition of acid(ASIC3)-induced chronic muscle pain in mice; wherein the ASIC3-dependent chronic muscle pain was induced by repeated injections of acid saline (pH 4.0, 20 ⁇ l) to one side of gastrocnemius muscle.
  • the first acid-injection induced a transient hyperalgesia in mouse hind paws that attenuated at 24 h, whereas the second acid injection (in 5 days) caused a bilateral long-lasting mechanical hyperalgesia for more than 2 weeks (left panel).
  • the co-injection of acid and T1-11 (middle panel) or APETx2 (a selective ASIC3 antagonist, right panel) abolished the transient hyperalgesia and prevented the development of chronic hyperalgesia induced by repeated acid injection.
  • FIG. 2 shows that the compound T1-11 induced an outward current in muscle nociceptors.
  • Panel A a diagram showing that the compound T1-11 elicited a slow inactivating outward current (I T1-11 ) in a dose-dependent manner in muscle nociceptor expressing SP-induced outward (I SP-O ).
  • FIG. 4 shows the effects of the compound CGS21680 on acid-induced muscle pain; wherein a co-injection of the compound CGS21680 (1 nmole, i.m.) and acidic saline partially inhibited acid-induced hyperalgesia, and a co-injection of an A3 agonist, IB-MECA (1 nmole, i.m.) as a control has no effect on acid-induced hyperalgesia.
  • FIG. 5 provides a schematic model of antinociception in ASIC3-expressing muscle nociceptors, showing that the adenosine analogs according to the invention, such as the compound T1-11, target on a novel analgesic pathway.
  • FIG. 6 shows that whole-cell patch clamp recordings revealed the inhibition of ASIC3-mediated current by the compound T1-11.
  • Pane A a diagram showing that no acid-induced current was found in CHO cells without ASIC3 transfection.
  • Panel B a diagram showing that the acid-induced current was blocked by salicylic acid (SA, a selective antagonist for ASIC3), and T1-11 in CHO cells transfected with ASIC3.
  • FIG. 7 shows that the compound T1-11 mediated currents in muscle DRG neurons; wherein the upper panel shows the T1-11-induced electrophysiological responses resulting from superfusion of 100 nM T1-11 for 4 s in GM-afferent DRG neurons and non-GM-afferent DRG neurons (including the number of neurons and percentage for each type of responses); and the lower panel shows the mean peak outward current of T1-11 (100 nM), that is similar to that of 3 ⁇ M substance P.
  • FIG. 8 shows that the compound T1-11 had the analgesic effect on a modified acid-induced chronic muscle pain model.
  • Panel A a diagram showing that mice were first received an intramuscular injection of genistein (a tyrosine kinase inhibitor) and an acid saline to one side of gastrocnemius muscle, and then pain behaviors were assayed by using von-Frey filament test at 4 hr (DO) and until 29 days (D29) after acid injection.
  • FIG. 9 shows the analgesic effect of the compound T1-11 on a mouse model of fibromyalgia; wherein T1-11 (30 ug/kg) had analgesic effect on mice treated with ICS.
  • Mechanical hyperalgesia was assessed by applying a 0.2 mN von Frey filament to the plantar surface of both hind paws. For each paw, the filament was applied for 5 times at 30-s intervals.
  • adenosine analogs which are effective for the treatment of pain through activation of neurokinin 1 (NK1) receptor signaling pathway in muscle nociceptors, thereby inducing the activation of activation of M-type potassium channel to induce outward currents. It is also found that the outward current is induced by M-type potassium channel in muscle afferent DRG neurons, and the activation of M-type potassium channel is mediated by NK1 receptor signaling in muscle afferent DRG neurons.
  • NK1 receptor 1 neurokinin 1
  • the invention provides a use in the manufacture of a medicament for treating pain of an adenonsine analog that activates NK1 receptor signaling, thereby inducing outward current.
  • mice lacking SP signaling were used to determine the contribution of SP to muscle pain sensitivity, and it is found that in contrast to the neurotransmitter's usual excitatory role, mice without SP signaling showed increased pain sensitivity after intramuscular acid injections as compared with mice that had normal SP signaling. Increased sensitivity to muscle pain was noted in mice lacking the gene for SP signaling as well as mice administered compounds designed to bind SP receptors.
  • FIG. 5 illustrates a schematic model of the T1-11 antinociception in ASIC3-expressing muscle nociceptors.
  • T1-11 acts on the NK1 associated with receptor in the local nerve terminals.
  • the NK1 receptors on muscle afferents are coupled with an unconventional signal pathway by activating M channels via a G-protein-independent, tyrosine kinase-dependent manner.
  • the invention provides a use in the manufacture of a medicament for treating pain of an adenonsine analog that activates NK1 receptor signaling, thereby inducing outward current.
  • the adenosine analog according to the invention is effective for treating acid-induced pain in term of the effects of T1-11 in inhibition of acid-sensing ion channel 3 (ASIC3) in muscle nociceptors. That is, the pain may include the pain associated with tissue acidosis, and the pain is associated with muscular origin, such as in muscle afferent DRG neurons. Accordingly, it is expected that the pain to be treated includes acid-induced pain, sich as acid-induced muscle pain, particularly an acid-induced chronic muscle pain.
  • ASIC3 acid-sensing ion channel 3
  • the pain may include inflammatory pain, cancer pain, chest pain, back pain, facial pain, joint pain, muscular pain syndromes, neuropathic pain, peripheral pain, cancer and tumor pain, sympathetic pain, postoperative pain, and post-traumatic pain.
  • the pain is fibromyalgia, myofascial pain, bladder pain syndrome or pain caused by irritable bowel syndrome.
  • adenosine analogs such as an adenosine analog having the formula T1-11 (“the compound T1-11” or “T1-11”), was proven to be effective in treating pain, particularly acid-induced pain.
  • the compound T1-11 is known as 2-(6-(4-hydroxybenzylamino)-9H-purin-9-yl)-5-(hydroxymethyl)-tetrahydrofuran-3,4-diol, which may be isolated from Gastrodia extract, such as from the roots. (See also U.S. Pat. No. 7,351,434 at col. 20, lines 4-22.)
  • the compound T1-11 was proven to activate neurokinin 1 (NK1) receptor signaling, and to be antinociceptive in ASIC3-mediated pain model.
  • a low dose of T1-11 (4 pmole) still had analgesic effects on acid-induced chronic muscle pain models in mice (see FIG. 1 ).
  • T1-11 mediated an outward current in SP-sensitive ASIC3-expressing muscle nociceptors and thus inhibited acid-induced ASIC3 activation (see FIG. 2(A) ).
  • the EC 50 of T1-11 for outward current is 1.3 nM as compared with SP of 2.6 ⁇ M (see FIGS. 2(B) & 2(C) ).
  • other adenosine analogs were also shown to mediate an outward current as potent as SP in muscle nociceptors (see Figure. 3).
  • the adenosine analog is a compound of Formula (I):
  • X is O, S or CH 2 ;
  • R 1 is selected from the group consisting of NHR 4 , NH(CH 2 ) n R 4 , NH—NHR 4 , NHCONHR 4 , NH—OR 4 , O—NHR 4 , and SR 4 ;
  • R 2 is selected from the group consisting of hydrogen (H), halogen, cyano, OR 4 , NHR 4 , substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl (Ar), substituted or unsubstituted aralkyl, and a substituted or unsubstituted heterocyclyl;
  • R 3 is selected from the group consisting of halomethyl, hydroxymethyl (HOCH 2 ), alkoxymethyl (R 4 OCH 2 ), azidomethyl (N 3 CH 2 ), aminomethyl (H 2 NCH 2 ), substituted or unsubstituted aminomethyl, amidomethyl (H 2 NCOCH 2 ), sulfanylmethyl (R 4 S), sulfonylmethyl (R 4 SO 2 ), triazolylmethyl, cyanomethyl (N ⁇ CCH 2 ), cyano, substituted or unsubstituted carbonyl (R 4 CO), COOH, substituted or unsubstituted aminocarbonyl (R 4 HNCO), substituted or unsubstituted alkynyl, and substituted or unsubstituted tetrazole;
  • n 1, 2 or 3;
  • each instance of R 4 is independently selected from the group consisting of H, alkyl, cycloalkyl, substituted or unsubstituted Ar, substituted or unsubstituted aralkyl, and a substituted or unsubstituted heterocyclyl;
  • Ar is selected from the group consisting of substituted or unsubstituted phenyl, substituted or unsubstituted polyarene, and a substituted or unsubstituted heterocycle.
  • R 2 is hydrogen. In some embodiments, R 2 is halogen. In some embodiments, R 2 is cyano. In some embodiments, R 2 is OR 4 . In some embodiments, R 2 is NHR 4 . In some embodiments, R 2 is substituted or unsubstituted alkyl. In some embodiments, R 2 is substituted or unsubstituted alkenyl. In some embodiments, R 2 is substituted or unsubstituted alkynyl. In some embodiments, R 2 is substituted or unsubstituted cycloalkyl. In some embodiments, R 2 is substituted or unsubstituted aryl.
  • R 2 is substituted or unsubstituted aralkyl.
  • R 2 is a hydrocarbon chain wherein the carbon unit is substituted by one or more aryl groups.
  • R 2 is C 1-10 hydrocarbon chain with the carbon unit substituted by one or more aryl groups.
  • R 2 is substituted or unsubstituted heterocyclyl.
  • R 2 is 5- or 6-membered ring or fused ring containing at least one nitrogen, oxygen or sulfur heteroatoms.
  • R 4 is substituted or unsubstituted aralkyl. In some embodiments, R 4 is a hydrocarbon chain with the carbon unit substituted by one or more aryl groups. In some embodiments, R 4 is a C 1-10 hydrocarbon chain with the carbon unit substituted by one or more aryl groups. In some embodiments, R 4 is substituted or unsubstituted heterocyclyl. In some embodiments, R 4 is 5- or 6-membered ring or fused ring containing at least one nitrogen, oxygen or sulfur heteroatoms.
  • Ar is substituted or unsubstituted phenyl. In some embodiments, Ar is substituted or unsubstituted polyarene. In some embodiments, Ar is substituted or unsubstituted heterocycle. In some embodiments, Ar is substituted or unsubstituted heterocycle of 5- or 6-membered ring or fused ring containing at least one nitrogen, oxygen or sulfur heteroatoms.
  • the adenosine analogs are respectively the compounds of Formula (II), Formula (III), and Formula (IV) as listed in Table 1 below, including but not limited to some examples, JMF1998, JMF2665, JMF1907 and CGS21680.
  • the compounds described herein include salts, solvates, hydrates forms thereof.
  • the compounds described herein can be prepared by conventional chemical transformations (including protecting group methodologies), e.g., those described in R. Larock, Comprehensive Organic Transformations, VCH Publishers (1989); T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 3rd Ed., John Wiley and Sons (1999); L. Fieser and M. Fieser, Fieser and Fieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995) and subsequent editions thereof.
  • a compound thus synthesized can be further purified by flash column chromatography, high performance liquid chromatography, crystallization, or any other suitable methods.
  • the adenosine analog described herein can be isolated from its natural source (e.g., extracted from a Gastrodia).
  • compound T1-11 can be isolated from a Gastradia extract.
  • the compounds mentioned herein may contain a non-aromatic double bond and one or more asymmetric centers. Thus, they can occur as racemates and racemic mixtures, single enantiomers, individual diastereomers, diastereomeric mixtures, and cis- or trans-isomeric forms. All such isomeric forms are contemplated.
  • compositions of the present invention and for use in accordance with the present invention may include a pharmaceutically acceptable excipient or carrier.
  • pharmaceutically acceptable carrier or “pharmaceutically acceptable excipient” means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type.
  • Remington's Pharmaceutical Sciences Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various excipients used in formulating pharmaceutical compositions and known techniques for the preparation thereof.
  • materials which can serve as pharmaceutically acceptable carriers are sugars such as lactose, glucose, and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose, and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; corn oil and soybean oil; glycols such as propylene glycol; esters such as ethyl oleate and ethyl laurate; agar; detergents such as Tween 80; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol; and phosphate buffer solutions, as well as other non-toxic compatible lubricants
  • compositions of this invention can be administered to humans and/or to animals, orally, rectally, parenterally, intracisternally, intravaginally, intranasally, intraperitoneally, topically (as by powders, creams, ointments, or drops), bucally, or as an oral or nasal spray.
  • Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups, and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan
  • sterile injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution, suspension, or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
  • acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid are used in the preparation of injectables.
  • the particles are suspended in a carrier fluid comprising 1% (w/v) sodium carboxymethyl cellulose and 0.1% (v/v) Tween 80.
  • the injectable formulations can be sterilized, for example, by filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
  • the rate of drug release can be controlled.
  • biodegradable polymers include poly(orthoesters) and poly(anhydrides).
  • Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions which are compatible with body tissues.
  • compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the particles with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol, or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the particles.
  • suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol, or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the particles.
  • Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
  • the particles are mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol,
  • compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols, and the like.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.
  • compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
  • Dosage forms for topical or transdermal administration of an inventive pharmaceutical composition include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants, or patches.
  • the particles are admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
  • Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention.
  • the pharmaceutically acceptable topical formulations of the invention comprise at least a compound of the invention and a penetration enhancing agent.
  • the choice of topical formulation will depend or several factors, including the condition to be treated, the physicochemical characteristics of the inventive compound and other excipients present, their stability in the formulation, available manufacturing equipment, and costs constraints.
  • penetration enhancing agent means an agent capable of transporting a pharmacologically active compound through the stratum coreum and into the epidermis or dermis, preferably, with little or no systemic absorption.
  • a wide variety of compounds have been evaluated as to their effectiveness in enhancing the rate of penetration of drugs through the skin. See, for example, Percutaneous Penetration Enhancers , Maibach H. I.
  • penetration agents for use with the invention include, but are not limited to, triglycerides (e.g., soybean oil), aloe compositions (e.g., aloe-vera gel), ethyl alcohol, isopropyl alcohol, octolyphenylpolyethylene glycol, oleic acid, polyethylene glycol 400, propylene glycol, N-decylmethylsulfoxide, fatty acid esters (e.g., isopropyl myristate, methyl laurate, glycerol monooleate, and propylene glycol monooleate), and N-methyl pyrrolidone.
  • triglycerides e.g., soybean oil
  • aloe compositions e.g., aloe-vera gel
  • ethyl alcohol isopropyl alcohol
  • octolyphenylpolyethylene glycol oleic acid
  • polyethylene glycol 400 propylene glycol
  • Transdermal patches have the added advantage of providing controlled delivery of a compound to the body.
  • dosage forms can be made by dissolving or dispensing the microparticles or nanoparticles in a proper medium.
  • Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the particles in a polymer matrix or gel.
  • the pharmaceutical compositions may be in the form of ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants. or patches.
  • formulations of the compositions according to the invention are creams, which may further contain saturated or unsaturated fatty acids such as stearic acid, palmitic acid, oleic acid, palmito-oleic acid, cetyl or oleyl alcohols, stearic acid being particularly preferred.
  • Creams of the invention may also contain a non-ionic surfactant, for example, polyoxy-40-stearate.
  • the active component is admixed under sterile conditions with a pharmaceutically acceptable excipient and any needed preservatives or buffers as may be required.
  • Ophthalmic formulations, eardrops, and eye drops are also contemplated as being within the scope of this invention.
  • the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms are made by dissolving or dispensing the compound in the proper medium.
  • penetration enhancing agents can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix (e.g., PLGA) or gel.
  • the ointments, pastes, creams, and gels may contain excipients such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc, and zinc oxide, or mixtures thereof.
  • Powders and sprays can contain excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates, and polyamide powder, or mixtures of these substances.
  • Sprays can additionally contain customary propellants such as chlorofluorohydrocarbons.
  • the compounds and pharmaceutical compositions of the invention can be formulated and employed in combination therapies, that is, the compounds and pharmaceutical compositions can be formulated with or administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures.
  • the particular combination of therapies (therapeutics or procedures) to employ in a combination regimen will take into account compatibility of the desired therapeutics and/or procedures and the desired therapeutic effect to be achieved. It will also be appreciated that the therapies employed may achieve a desired effect for treating pain.
  • a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable salts, esters, salts of such esters, or a prodrug or other adduct or derivative of a compound of this invention which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
  • the invention also provides a pharmaceutical composition for treating pain, comprising:
  • an adenosine analog as defined herein e.g., formula I, II, III, or IV such as compound T1-11, JMF1907, JMF1998, JMF2665, and CGS21680
  • formula I, II, III, or IV such as compound T1-11, JMF1907, JMF1998, JMF2665, and CGS21680
  • the present invention provides a method for treating pain comprising administering to a subject in need thereof a therapeutically effective amount of an adenosine analog, or a pharmaceutical composition as defined herein.
  • the compounds according to the invention can enhance outward current induced by M-type potassium channel in muscle neurons.
  • the activation of NK1 receptor signaling produces beneficial therapeutic effects on pain management.
  • the pain to be treated may be associated with cardiovascular disease, stroke-induced neural damage, arthritis, cancer, inflammation, infection, oropharengeal diseases or damage, traumatic injuries, acute and chronic cough, gastrointestinal disorders, central nervous system disorders, psychiatric diseases or manifestations.
  • the compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
  • dosage unit form refers to a physically discrete unit of therapeutic agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment.
  • the specific therapeutically effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex, and diet of the subject; the time of administration, mute of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and like factors well known in the medical arts (see, for example, Goodman and Gilman's The Pharmacological Basis of Therapeutics , Tenth Edition, A. Gilman, J. Hardman and L. Limbird, eds., McGraw-Bill Press, 155-173, 2001, which is incorporated herein by reference in its entirety).
  • mice were treated with intermittent cold stress, which produces long-lasting (more than 2 weeks) thermal hyperalgesia and mechanical allodynia, predominantly in females.
  • Method Mice were placed on stainless mesh plate in a cold room at 4° C. overnight (from 4:30 pm to 10:00 am), followed by intermittent cold stress with environment temperatures alternating between 24 and 4° C. every 30 min, from 10:00 am to 4:30 pm. These procedures were repeated twice. On day 3, the mice were adapted to 24 C for 1 h before behavioral testing.
  • mice received an intramuscular acid injection in the gastrocnemius muscle (GM) containing 20 pi acid saline (pH 4.0) with or without (Sar 9 , Met(O 2 ) 11 )-substance P (SP; 4, 10, or 40 ⁇ M) or 100 ⁇ M RP67580 or 200 ⁇ M XE991.
  • Mechanical hyperalgesia was assessed by applying a 0.2-mN von Frey filament to the plantar surface of both hind paws of mice. Mice were acclimatized in an acrylic cubicle for 60 min before testing. A positive response was defined as the foot lifting when the von Frey filament was applied. For each paw, the filament was applied 5 times at 30-sec intervals.
  • NK1-selective agonist ((Sar 9 , Met(O 2 ) 11 )-SP) was from Sigma Chemical (St. Louis, Mo.).
  • NK1-selective antagonist (RP67580) and XE991 were from Tocris (Avonmouth, UK). The experimenters were blinded to the experimental manipulations. The person who conducted von Frey test had no information of the mouse genotypes or drug injections.
  • mice were intraperitoneally injected with 1% Evans Blue dye (EB, E2129-10 G Sigma) (W/V in phosphate buffered saline), which was sterilized by passage through Millex-GS 0.22- ⁇ m filter (Millipore). Then 24 hr later, mice were injected with 20 ⁇ l solution (pH 7.4 saline, pH 4.0 saline, 40 ⁇ M SM-SP, or 20% mustard oil) into the left GM. At 30 min after intramuscular injection, the right and left GM were individually dissected, weighed, diced, and placed in 4 ml of 99% formamide at room temperature (21-25° C.) for 72 hr.
  • EB Evans Blue dye
  • pH 4.0 saline pH 4.0 saline
  • 40 ⁇ M SM-SP 20% mustard oil
  • the samples were centrifuged at 2,000 rpm for 20 min. The absorbance of the supernatant was determined spectrophotometrically at 620-nm wavelength. The dye content of samples was calculated from a standard curve of Evans Blue concentrations. Comparison between groups involved Mann-Whitney test. Drugs were purchased from Sigma Chemical.
  • DRG Dorsal Root Ganglion Primary Culture.
  • mice were injected with 4% Fluorogold (Fluorochrome, Denver, Colo.) into the GM of both legs for 5 to 7 days, then lumbar DRG neurons were dissected and removed from both sides and placed in a tube for digestion with 1 ml DMEM containing 0.125% type 1 collagenase for 90 min and 0.25% trypsin for 20 min at 37° C.
  • the digested DRG neurons were washed with fetal calf serum (FCS)-free DMEM or DMEM containing 10% FCS during each treatment. Fully digested DRGs were triturated and plated on poly-L-lysine-coated cover slides. Cell cultures were maintained in a 5% CO2 incubator at 37° C.
  • FCS fetal calf serum
  • Whole-Cell Patch-Clamp Recording Whole-cell patch-clamp recordings of muscle-afferent neurons involved use of Axopatch MultiClamp 700B (Axon Instruments). Neurons with membrane potential > ⁇ 40 mV were discarded. The bridge was balanced in current-clamp recording, and the series resistance was compensated 70% in voltage-clamp recording with Axopatch 700B compensation circuitry. All experiments were performed at room temperature (21-25° C.) and completed within 30 hr post-seeding.
  • the patch pipettes were prepared in 1-5 M ⁇ and filled with internal solution containing (in mM) 100 KCl, 2 Na z -ATP, 0.3 Na 3 -GTP, 10 EGTA, 5 MgCl 2 , and 40 HEPES, adjusted to pH 7.4 with KOH.
  • Recording cells were superfused in artificial cerebrospinal fluid (ACSF) by control with gravitational force.
  • the ACSF contained (in mM) 130 NaCl, 5 KCl, 1 MgCl 2 , 2 CaCl 2 , 10 glucose, and 20 HEPES, adjusted to pH 7.4 with NaOH. Osmolarity was adjusted to 300 mOsm.
  • the acidic ACSF was titrated to pH 6.8 by 1 M NaOH.
  • SP was prepared from a 300- ⁇ M stock solution to a final concentration of 3 ⁇ M in ACSF.
  • NaCl was replaced with Tetraethyl ammonium chloride and pH was adjusted with CsOH.
  • drugs listed above were from Sigma Chemical (St. Louis, Mo.).
  • Reagents used in the study of SP-induced outward current were from Sigma (GDP- ⁇ -S) or Tocris Bioscience (RP67580, GR159897, SB218795, genistein, PP1, sodium orthovanadate, daidzein, XE991 and Linopirdine dihydrochloride).
  • Voltage-clamp mode was used to examine the effect of SP on acid-induced currents in ASIC3-expressing DRG neurons.
  • a total of 40 GM and 6 non-GM DRG neurons were recorded in voltage-clamp mode with Vm held at ⁇ 70 mV.
  • SA salicylic acid
  • SAS salicylic acid-sensitive
  • acid-induced current was first recorded in a bath containing 10 ⁇ M RP67580 for 2 min as a control response. Next, the recording was performed in the bath containing both RP67580 and SP for 2 min, then one containing RP67580 alone. Phosphotyrosine kinase was tested for its role in SP-directed modulation on acid-induced current. Acid-induced current was recorded in a bath containing both SP and genistein (30 ⁇ M; Tocris), or both SP and daidzein (30 ⁇ M; Tocris) for 3 min. In addition, recording was performed in SP- and genistein-only bath as positive and negative controls, respectively.
  • SP-Induced Currents Voltage-clamp mode was used to detect SP-induced currents, and neurons were held at ⁇ 70 mV.
  • SP in ACSF solutions (3 ⁇ M) was puffed through a valve controller-controlled glass pipette towards the recording neurons for 4 sec with a 30-sec interval. The procedure was repeated at least 2 times to ensure consistent data.
  • the SP-induced current tested under different pharmacological compounds was performed in triplicate. ACSF-containing compounds were superfused for 1 min to ensure complete replacement of previous perfusate between treatments. Then, the test compounds were washed away with ACSF, and SP-induced currents after the washout were examined 3 times.
  • a neuron was defined as SP sensitive when the induced current was >10 pA (or ⁇ 10 pA with an outward current).
  • Baclofen-induced GABA-B current served as a positive control.
  • a recording pipette containing 1 mM GDP- ⁇ -S was used in GTP dialysis.
  • the GABA-B current was induced by 100 ⁇ M Baclofen at the beginning of dialysis (0, 1 and 2 min after whole-cell patch clamp). Then, Baclofen was used to re-stimulate the dialyzed neurons after 10-min dialysis (10, 11 and 12 min after whole-cell patch clamp). Then, SP was used to verify the presence of I SP-O in the absence of GTP.
  • mice were first injected with retrograde tracer 2 days before the injection of acidic saline (pH 4.0, 20 ⁇ l) or in combination with 100 ⁇ M RP67580 or 200 ⁇ M XE991. The treated mice were then killed and used for DRG culture 2 days later. For mice that received 2 injections of pH 4.0 saline, the second injection was given 2 days after the first injection and the mice were also killed 2 days after the second injection. DRG culture was as stated above and used for studying the voltage-gated sodium currents.
  • the internal solution contained (in mM) 10 NaCl, 110 CsCl, 20 tetraethylammonium-Cl, 2.5 MgCl 2 , 5 EGTA, 3 Mg 2 + -ATP, and 5 HEPES, adjusted to pH 7.0 with CsOH.
  • the external solution for voltage-gated sodium current contained (in mM) 100 NaCl, 5 CsCl, 30 tetraethylammonium-Cl, 1.8 CaCl 2 , 1 MgCl 2 , 0.1 CdCl 2 , 25 glucose, 5 4-aminopyridine, and 5 HEPES, adjusted to pH 7.4 with HCl. Osmolarity was adjusted to approximately 300 mOsm with glucose.
  • DRG neurons with cell diameter between 30 ⁇ 40 ⁇ m were selected for recording.
  • the voltage-gated sodium currents were evoked by a 30-msec test pulse at ⁇ 40 mV from a holding potential of ⁇ 80 mV. Recordings were performed in external solution with or without 200 nM tetrodotoxin (TTX; Tocris Bioscience, Avonmouth, UK).
  • Action Potential Threshold The action potentials of GM DRG neurons of different experimental groups were evoked by various voltages, and the threshold was defined as the beginning of the steep upward rise of the action potential.
  • T1-11 had analgesic effect on acid-induced chronic muscle pain, in which the muscle pain was induced by repeated injection of acid saline (pH 4.0, 20 ⁇ l) to one side of gastrocnemius muscle (GM).
  • the first acid injection induced rapid, transient referred hyperalgesia, which declined after 24 h; a second acid injection administered 5 day after the first induced long-lasting (>2 weeks) referred hyperalgesia.
  • the acid-induced muscle pain is ASIC3-dependent.
  • Co-injection of acid with ASIC3 antagonist APETx2 (2 pmole) at the first injection abolished the development of chronic hyperalgesia induced by the second acid injection.
  • T1-11 (4 pmole) has the similar potency with APETx2 in inhibition of the acid-induced chronic hyperalgesia ( FIG. 1 ).
  • T1-11 induces an outward current (I T1-11 ) in ASIC3-positive muscle afferent DRG neurons that also express substance P (SP)-induced outward current (I SP-O ).
  • SP substance P
  • I SP-O substance P-induced outward current
  • T1-11 analogues such as JMF1998, 1907, 2665, CGS21680, can also induce outward currents in the ASIC3/SP-expressing muscle DRG neurons (see FIGS. 3 and 4 ).
  • Co-injection of A2AR agonist CGS21680 (I nmole, i.m.) and acidic saline partially inhibited acid-induced hyperalgesia, whereas co-injection of A3 agonist IB-MECA (1 nmole, i.m.) has no effect on acid-induced hyperalgesia.
  • T1-11 antinociception in muscle nociceptors was concluded and illustrated in FIG. 5 .
  • tissue acidosis occurs in muscle, protons depolarize the muscle afferents.
  • T1-11 acts on the NK1-associated receptor in the local nerve terminals.
  • the NK1-associated receptors on muscle afferents are coupled with an unconventional signal pathway by activating M channels via a G-protein-independent, tyrosine kinase-dependent manner.
  • the upper panel shows T1-11-mediated currents found in muscle DRG neurons (see the upper panel), and T1-11-induced electrophysiological responses resulting from superfusion of 100 nM T1-11 for 4 s in GM-afferent DRG neurons and non-GM-afferent DRG neurons, including the number of neurons and percentage for each type of responses.
  • the lower panel shows the mean peak outward current of T1-11 (100 nM) is similar to that of 3 ⁇ M substance P.
  • the I T1-11 is predominantly expressed in muscle afferent DRG neurons but not other sensory neurons.
  • I T1-11 is reversely inhibited by A3 adenosine receptor antagonists (MRS1220, MRE3008F20) but not by A2A adenosine receptor antagonist (ZM241385), indicating the involvement of A3 adenosine receptors.
  • MRS1220, MRE3008F20 A3 adenosine receptor antagonist
  • ZM241385 A2A adenosine receptor antagonist
  • T1-11 was known bound to A2A adenosine receptors in neurons of central nervous system, we showed that I T1-11 is not changed in A2A ⁇ / ⁇ muscle afferent DRG neurons.
  • A3 adenosine receptor antagonist (MRS1220, 200 pmole i.m.) abolished the analgesic effect of T1-11 on acid-induced muscle pain model, which further demonstrates the involvement of A3 adenosine receptors in I T1-11 .
  • A3 adenosine receptor agonist (IB-MECA, 1 nmole i.m.) did not have analgesic effect on acid-induced muscle pain.
  • A2A adenosine receptor agonist (CGS21680, 1 nmole i.m.) showed partial analgesic effect on the acid-induced muscle pain ( FIG. 4 ). Accordingly, CGS21680 in high dose (10 ⁇ M) induces an outward current in A2A ⁇ / ⁇ muscle afferent DRG neurons, indicating CGS21680 is a partial agonist for the I T1-11 .
  • I T1-11 is resistant to GTP dialysis and mediated via M-type potassium channel.
  • T1-11 enhanced M current in voltage shift from ⁇ 50 mV to ⁇ 20 mV in muscle afferent neurons expressing I SP-O .
  • T1-11 enhanced M-type potassium currents through the NK1-associated receptors in a G-protein-independent manner.
  • A3 adenosine receptors were proven to be involved in the NK1-associated receptor complex.
  • T1-11 was an excellent therapeutic compound in two mouse models of fibromyalgia.
  • the first model was the acid-induced chronic muscle pain model, in which mice developed chronic muscle after intramuscular acid injection and a genistein treatment (see FIG. 8 ).
  • T1-11 showed a dose-dependent analgesic effect on mice that have developed the chronic muscle pain wherein the doses of T1-11 were in the range of 75-150 ⁇ g/kg (i.p.).
  • the second fibromyalgia model is developed by Ueda's group, in which mice were treated with intermittent cold stress for 2 days (Nishiyori & Ueda, 2008). Mice treated with intermittent cold stress will develop long-lasting (>2 weeks) mechanical and thermal hyperalgesia.
  • Analgesic effect of T1-11 was tested in these mice 5 day after intermittent cold stress (ICS). We found that T1-11 in 30 ⁇ g/kg (i.p.) was the therapeutic dose to treat the pain (see FIG. 9 ). Moreover, we found T1-11 and substance P have synergistic effect in treating the ICS-induced pain.
  • T1-11 in 3 ⁇ g/kg or SM-substance P in 0.6 mg/kg (a selective agonist for NK1 receptor) alone did not show analgesic effect on the ICS-treated mice.
  • SM-substance P in 0.6 mg/kg a selective agonist for NK1 receptor

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