TWI329743B - - Google Patents

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Publication number: TWI329743B
Authority: TW; Taiwan
Prior art keywords: cervical; methylation; target gene; seq; dna
Prior art date: 2007-01-23

Application number

TW96102422A

Other languages

English (en)

Chinese (zh)

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TW200831900A (en

Original Assignee

Nat Defense Medical Ct

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2007-01-23

Filing date

2007-01-23

Publication date

2010-09-01

2007-01-23 Application filed by Nat Defense Medical Ct filed Critical Nat Defense Medical Ct

2007-01-23 Priority to TW96102422A priority Critical patent/TW200831900A/zh

2008-08-01 Publication of TW200831900A publication Critical patent/TW200831900A/zh

2010-09-01 Application granted granted Critical

2010-09-01 Publication of TWI329743B publication Critical patent/TWI329743B/zh

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Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

TW96102422A 2007-01-23 2007-01-23 Cancer screen method TW200831900A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
TW96102422A TW200831900A (en)	2007-01-23	2007-01-23	Cancer screen method

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
TW96102422A TW200831900A (en)	2007-01-23	2007-01-23	Cancer screen method

Publications (2)

Publication Number	Publication Date
TW200831900A TW200831900A (en)	2008-08-01
TWI329743B true TWI329743B (fr)	2010-09-01

Family

ID=44818727

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
TW96102422A TW200831900A (en)	2007-01-23	2007-01-23	Cancer screen method

Country Status (1)

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TW (1)	TW200831900A (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
TWI385252B (zh) *	2009-04-16	2013-02-11	Nat Defense Medical Ct	一種癌症篩檢的方法
DK2420578T3 (en)	2009-04-17	2017-10-02	Nat Defense Medical Center	A method of screening for cancer
US8048634B2 (en)	2009-08-18	2011-11-01	National Defense Medical Center	Cancer screening method
HK1210227A1 (en)	2012-08-28	2016-04-15	日祥生命科学股份有限公司	Test composition for screening cancers
TWI513822B (zh) *	2012-08-28	2015-12-21		一種癌症篩檢的檢驗套組
EP2891720B1 (fr)	2012-08-31	2020-04-08	National Defense Medical Center	Méthode de dépistage du cancer

2007
- 2007-01-23 TW TW96102422A patent/TW200831900A/zh unknown

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EP2235218B1 (fr)	2014-11-19	Methode, procede, et kit de diagnostic, pronostic du cancer colorectal
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