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SE8305327L - Isolering av differentierade celler - Google Patents

Isolering av differentierade celler

Info

Publication number
SE8305327L
SE8305327L SE8305327A SE8305327A SE8305327L SE 8305327 L SE8305327 L SE 8305327L SE 8305327 A SE8305327 A SE 8305327A SE 8305327 A SE8305327 A SE 8305327A SE 8305327 L SE8305327 L SE 8305327L
Authority
SE
Sweden
Prior art keywords
dna
sub
cells
sequence
microorganisms
Prior art date
Application number
SE8305327A
Other languages
English (en)
Other versions
SE8305327D0 (sv
Inventor
W J Rutter
H M Goodman
A Ullrich
J Shine
J M Chirgwin
R L Pictet
P H Seeburg
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05897710 external-priority patent/US4363877B1/en
Priority claimed from US05/898,887 external-priority patent/US4264731A/en
Application filed by Univ California filed Critical Univ California
Publication of SE8305327L publication Critical patent/SE8305327L/sv
Publication of SE8305327D0 publication Critical patent/SE8305327D0/sv

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57518—Placental lactogen; Chorionic somatomammotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Diabetes (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (1)

1. Patentkrav Forfarande for isolering av cellar fran Langerhans bar (islet cells), innehallande mRNA, son ar anrikad pa den nukleotidsekvens, som kodar for insulin, kannetecknat av att bukspottkortelvavnad innehallande Oceller och andra cellar behandlas med ett hydrolytiskt enzym och en trypsininhibitor fbr Ott darigenom frigora barna fran.de andra cellerna, den behandlade vavnaden fraktioneras for att darigenom barns shall ernallas i huvudsak trim fran andra celler och fran stinderbnm:tet cellmaterial, Iran dessa bar isolerar man mRNA, vilken ar anrikad pa den nukleotidsekvens, son kodar fbr insulin.
SE8305327A 1977-05-27 1983-09-29 Isolering av differentierade celler SE8305327D0 (sv)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US80134377A 1977-05-27 1977-05-27
US80502377A 1977-06-09 1977-06-09
US89770978A 1978-04-19 1978-04-19
US05897710 US4363877B1 (en) 1977-09-23 1978-04-19 Recombinant dna transfer vectors
US05/898,887 US4264731A (en) 1977-05-27 1978-04-21 DNA Joining method
US89900378A 1978-04-26 1978-04-26
US89900278A 1978-04-26 1978-04-26

Publications (2)

Publication Number Publication Date
SE8305327L true SE8305327L (sv) 1983-09-29
SE8305327D0 SE8305327D0 (sv) 1983-09-29

Family

ID=27569921

Family Applications (4)

Application Number Title Priority Date Filing Date
SE7806086A SE451461B (sv) 1977-05-27 1978-05-26 Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli
SE8305327A SE8305327D0 (sv) 1977-05-27 1983-09-29 Isolering av differentierade celler
SE8305329A SE455794B (sv) 1977-05-27 1983-09-29 Plasmid med nukleotidsekvens som kodar for aminosyrasekvensen i humant tillvexthormon och forfarande for framstellning derav
SE8305328A SE8305328L (sv) 1977-05-27 1983-09-29 Isolering av icke nedbruten rna

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SE7806086A SE451461B (sv) 1977-05-27 1978-05-26 Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli

Family Applications After (2)

Application Number Title Priority Date Filing Date
SE8305329A SE455794B (sv) 1977-05-27 1983-09-29 Plasmid med nukleotidsekvens som kodar for aminosyrasekvensen i humant tillvexthormon och forfarande for framstellning derav
SE8305328A SE8305328L (sv) 1977-05-27 1983-09-29 Isolering av icke nedbruten rna

Country Status (21)

Country Link
JP (1) JPH0659218B2 (sv)
AR (1) AR225404A1 (sv)
AU (1) AU521903B2 (sv)
BE (1) BE867424A (sv)
BG (1) BG40319A3 (sv)
CH (1) CH642680A5 (sv)
DD (1) DD145927A5 (sv)
DE (2) DE2822568A1 (sv)
DK (2) DK233278A (sv)
FI (1) FI64640C (sv)
FR (1) FR2392033B1 (sv)
GB (1) GB1565190A (sv)
GR (1) GR73552B (sv)
IL (1) IL54790A (sv)
IT (1) IT1094934B (sv)
LU (1) LU79714A1 (sv)
NL (1) NL7805591A (sv)
NZ (1) NZ187300A (sv)
PL (1) PL140200B1 (sv)
PT (1) PT68082B (sv)
SE (4) SE451461B (sv)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA782933B (en) * 1977-09-23 1979-05-30 Univ California Purification of nucleotide sequences suitable for expression in bacteria
BR7807290A (pt) * 1977-11-08 1979-06-12 Genentech Inc Plasmideo,processo para producao de um polipeptido especifico veiculo clonal,cultura bacteriana transformante,processo para produzir uma substancia imunogenica processo para preparar um gene estrutural,e polidesoxirribonucleotido de faixa dupla
GR71912B (sv) * 1978-08-11 1983-08-16 Univ California
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production
JPS55150883A (en) * 1979-05-11 1980-11-25 Nakano Vinegar Co Ltd Method of storing "mozuku"
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
FR2458584A1 (fr) * 1979-06-08 1981-01-02 Pasteur Institut Vecteurs pour le transfert et l'expression de materiel genetique dans une cellule eucaryote et procede pour la production d'une proteine determinee dans des cellules eucaryotes
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
GR70279B (sv) * 1979-09-12 1982-09-03 Univ California
IL58765A (en) * 1979-11-21 1986-09-30 Yeda Res & Dev Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
DE3001928A1 (de) * 1980-01-19 1981-08-06 Hoechst Ag, 6000 Frankfurt Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
CA1202581A (en) * 1980-03-27 1986-04-01 Saran A. Narang Adaptor molecules for dna and their application to synthesis of gene-derived products
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
BR8108074A (pt) * 1980-12-12 1982-09-21 Unilever Nv Sequencia de dna,plasmidio recombinante,ligadores de dna,cultura bacterial e processo para produzir taumatina
EP0054331B1 (en) * 1980-12-12 1992-06-03 Unilever N.V. Structural genes encoding the various allelic and maturation forms of preprothaumatin and mutations thereof, recombinant cloning vehicles comprising said structural genes and expression thereof in transformed microbial host cells
US4695543A (en) * 1982-03-23 1987-09-22 Bristol-Myers Company Alpha Interferon GX-1
US4748233A (en) * 1982-03-23 1988-05-31 Bristol-Myers Company Alpha-interferon Gx-1
EP0103395A3 (en) * 1982-08-17 1985-05-22 Biogen N.V. Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield
US5474980A (en) * 1984-10-04 1995-12-12 Monsanto Company Prolonged release of biologically active somatotropins
US5411951A (en) * 1984-10-04 1995-05-02 Monsanto Company Prolonged release of biologically active somatotropin
DK257988D0 (da) * 1988-05-11 1988-05-11 Novo Industri As Nye peptider
DE4034702A1 (de) * 1990-10-31 1992-05-07 Siemens Ag Verfahren und vorrichtung zur pruefung der aktiven werkzeuge von gesenkbiegemaschinen, insbesondere von biegestempeln

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment

Also Published As

Publication number Publication date
IT1094934B (it) 1985-08-10
DK791A (da) 1991-01-04
AR225404A1 (es) 1982-03-31
FI64640C (fi) 1983-12-12
PL140200B1 (en) 1987-04-30
SE8305328D0 (sv) 1983-09-29
DK791D0 (da) 1991-01-04
DD145927A5 (de) 1981-01-14
JPS5449387A (en) 1979-04-18
CH642680A5 (en) 1984-04-30
JPH0659218B2 (ja) 1994-08-10
SE8305329D0 (sv) 1983-09-29
DK233278A (da) 1978-11-28
BE867424A (fr) 1978-09-18
SE8305328L (sv) 1983-09-29
PT68082A (en) 1978-06-01
FR2392033A1 (fr) 1978-12-22
SE8305329L (sv) 1983-09-29
PT68082B (en) 1980-02-20
DE2822568C2 (sv) 1989-08-31
IL54790A (en) 1983-07-31
IT7823930A0 (it) 1978-05-29
NZ187300A (en) 1982-08-17
IL54790A0 (en) 1978-07-31
DE2822568A1 (de) 1978-12-14
GR73552B (sv) 1984-03-14
GB1565190A (en) 1980-04-16
FR2392033B1 (fr) 1985-09-13
AU3648878A (en) 1979-11-29
PL207176A1 (pl) 1979-03-26
NL7805591A (nl) 1978-11-29
SE7806086L (sv) 1978-11-28
SE451461B (sv) 1987-10-12
SE455794B (sv) 1988-08-08
LU79714A1 (fr) 1978-11-06
DE2858357C2 (sv) 1990-03-15
FI781675A7 (fi) 1978-11-28
AU521903B2 (en) 1982-05-06
SE8305327D0 (sv) 1983-09-29
FI64640B (fi) 1983-08-31
BG40319A3 (bg) 1986-11-14

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