RU2017125728A - Культивирование плюрипотентных стволовых клеток в суспензии - Google Patents
Культивирование плюрипотентных стволовых клеток в суспензии Download PDFInfo
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- RU2017125728A RU2017125728A RU2017125728A RU2017125728A RU2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A RU 2017125728 A RU2017125728 A RU 2017125728A
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- cells
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- endoderm
- ptf1a
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- 210000001778 pluripotent stem cell Anatomy 0.000 title 1
- 239000000725 suspension Substances 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims 13
- 210000004039 endoderm cell Anatomy 0.000 claims 9
- 238000000034 method Methods 0.000 claims 7
- 101000738523 Homo sapiens Pancreas transcription factor 1 subunit alpha Proteins 0.000 claims 5
- 102100037878 Pancreas transcription factor 1 subunit alpha Human genes 0.000 claims 5
- 230000014509 gene expression Effects 0.000 claims 5
- 230000000968 intestinal effect Effects 0.000 claims 5
- 238000012258 culturing Methods 0.000 claims 4
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims 3
- 102100038553 Neurogenin-3 Human genes 0.000 claims 3
- 210000001900 endoderm Anatomy 0.000 claims 3
- 230000019491 signal transduction Effects 0.000 claims 3
- 238000004114 suspension culture Methods 0.000 claims 2
- 102000003693 Hedgehog Proteins Human genes 0.000 claims 1
- 108090000031 Hedgehog Proteins Proteins 0.000 claims 1
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims 1
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims 1
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims 1
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 230000004186 co-expression Effects 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 210000003890 endocrine cell Anatomy 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 108091006086 inhibitor proteins Proteins 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 210000000936 intestine Anatomy 0.000 claims 1
- 230000009996 pancreatic endocrine effect Effects 0.000 claims 1
- 150000004492 retinoid derivatives Chemical class 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims 1
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Claims (7)
1. Способ дифференцировки человеческих плюрипотентных клеток, включающий стадии: дифференцировки клеток энтодермы передней кишки в клетки панкреатической энтодермы посредством культивирования клеток энтодермы передней кишки в динамической суспензионной культуре при рН от около 7,2 до около 7,0 в течение, по меньшей мере, около 24 часов.
2. Способ по п. 1, дополнительно включающий культивирование клеток энтодермы передней кишки в культуре, в которой концентрация клеток равна или превышает около 1,5 миллиона клеток/мл.
3. Способ по п. 1, дополнительно включающий культивирование клеток энтодермы передней кишки в культуре, в которой концентрация клеток превышает или равна около 2,0 миллиона клеток/мл.
4. Способ по п. 1, в котором клетки панкреатической энтодермы являются по существу отрицательными в отношении экспрессии PTF1A и NGN3.
5. Способ по п. 4, дополнительно включающий обогащение клеток панкреатической энтодермы, которые являются по существу отрицательными в отношении экспрессии PTF1A и NGN3, до популяции клеток панкреатической энтодермы, имеющей около 96% или более клеток, которые являются положительными в отношении совместной экспрессии PDX1 и NKX6.1 и положительными в отношении экспрессии PTF1A.
6. Способ по п. 4, дополнительно включающий дифференцировку клеток панкреатической энтодермы, которые являются по существу отрицательными в отношении экспрессии PTF1A и NGN3, до панкреатических эндокринных клеток при отсутствии стадии дифференцировки, на которой формируются клетки, положительные в отношении экспрессии PTF1A.
7. Способ дифференцировки человеческих плюрипотентных клеток, включающий стадии: дифференцировки клеток энтодермы передней кишки в клетки панкреатической энтодермы посредством культивирования клеток энтодермы передней кишки в динамической суспензионной культуре при рН от около 7,2 до около 7,0 в течение, по меньшей мере, около 24 часов, при концентрации клеток, равной или превышающей около 1,5 миллиона клеток/мл и концентрации ретиноида от около 0,5 до около 1,0 мкМ, причем культивирование выполняют при отсутствии компонентов для одного или более из ингибирования, блокирования, активации или стимулирования сигнального пути TGF-бета и сигнального пути BMP и ингибитора сигнального пути белка sonic hedgehog.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462094509P | 2014-12-19 | 2014-12-19 | |
| US62/094,509 | 2014-12-19 | ||
| PCT/US2015/064713 WO2016100035A1 (en) | 2014-12-19 | 2015-12-09 | Suspension culturing of pluripotent stem cells |
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| Publication Number | Publication Date |
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| RU2017125728A true RU2017125728A (ru) | 2019-01-22 |
| RU2017125728A3 RU2017125728A3 (ru) | 2019-06-03 |
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| RU2017125728A RU2017125728A (ru) | 2014-12-19 | 2015-12-09 | Культивирование плюрипотентных стволовых клеток в суспензии |
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| US (1) | US20160215268A1 (ru) |
| EP (1) | EP3234109A4 (ru) |
| JP (1) | JP6800854B2 (ru) |
| KR (3) | KR102281752B1 (ru) |
| CN (1) | CN107250349A (ru) |
| AR (1) | AR103080A1 (ru) |
| AU (1) | AU2015363008B2 (ru) |
| BR (1) | BR112017012974A2 (ru) |
| CA (1) | CA2970935A1 (ru) |
| MX (1) | MX2017008176A (ru) |
| PH (1) | PH12017501153A1 (ru) |
| RU (1) | RU2017125728A (ru) |
| SG (1) | SG11201704961VA (ru) |
| TW (1) | TW201636421A (ru) |
| WO (1) | WO2016100035A1 (ru) |
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| JP7510247B2 (ja) | 2016-01-26 | 2024-07-03 | センター フォー コマーシャリゼーション オブ リジェネレイティブ メディスン | 細胞凝集塊を解離させる方法 |
| US10391156B2 (en) | 2017-07-12 | 2019-08-27 | Viacyte, Inc. | University donor cells and related methods |
| CN111246864A (zh) * | 2017-07-21 | 2020-06-05 | 森玛治疗公司 | 干细胞衍生的胰腺β细胞的重聚 |
| CA3074910A1 (en) * | 2017-09-11 | 2019-03-14 | Novo Nordisk A/S | Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells |
| CA3081762A1 (en) * | 2017-11-15 | 2019-05-23 | Semma Therapeutics, Inc. | Islet cell manufacturing compositions and methods of use |
| WO2020033879A1 (en) | 2018-08-10 | 2020-02-13 | Semma Therapeutics, Inc. | Stem cell derived islet differentiation |
| US12378572B2 (en) | 2018-09-07 | 2025-08-05 | Crispr Therapeutics Ag | Universal donor cells |
| TWI862513B (zh) * | 2018-09-19 | 2024-11-21 | 日商千紙鶴治療公司 | 包含胰島素產生細胞之細胞集團、包含該細胞集團之醫藥、其用途及製造方法 |
| CN113728090B (zh) * | 2019-04-08 | 2024-04-05 | 诺和诺德股份有限公司 | 从干细胞衍生的定形内胚层生成胰腺内胚层 |
| CN114173837A (zh) | 2019-05-31 | 2022-03-11 | W.L.戈尔及同仁股份有限公司 | 生物相容性膜复合材料 |
| US20220233298A1 (en) | 2019-05-31 | 2022-07-28 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| WO2020243663A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | A biocompatible membrane composite |
| CA3139292C (en) | 2019-05-31 | 2025-06-10 | Viacyte, Inc. | CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES |
| EP4025224A1 (en) | 2019-09-05 | 2022-07-13 | CRISPR Therapeutics AG | Universal donor cells |
| EP4025690A1 (en) | 2019-09-05 | 2022-07-13 | CRISPR Therapeutics AG | Universal donor cells |
| KR20230146008A (ko) | 2020-12-31 | 2023-10-18 | 크리스퍼 테라퓨틱스 아게 | 범용 공여자 세포 |
| CN119432595A (zh) * | 2023-08-07 | 2025-02-14 | 浙江霍德生物工程有限公司 | 利用生物反应器进行诱导多能干细胞的悬浮培养 |
| WO2025117331A1 (en) * | 2023-12-01 | 2025-06-05 | Eli Lilly And Company | Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same |
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| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
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-
2015
- 2015-12-09 KR KR1020177019548A patent/KR102281752B1/ko active Active
- 2015-12-09 RU RU2017125728A patent/RU2017125728A/ru not_active Application Discontinuation
- 2015-12-09 MX MX2017008176A patent/MX2017008176A/es unknown
- 2015-12-09 CN CN201580076646.0A patent/CN107250349A/zh active Pending
- 2015-12-09 SG SG11201704961VA patent/SG11201704961VA/en unknown
- 2015-12-09 BR BR112017012974A patent/BR112017012974A2/pt not_active Application Discontinuation
- 2015-12-09 KR KR1020237011455A patent/KR102700499B1/ko active Active
- 2015-12-09 AU AU2015363008A patent/AU2015363008B2/en active Active
- 2015-12-09 JP JP2017532641A patent/JP6800854B2/ja active Active
- 2015-12-09 US US14/963,730 patent/US20160215268A1/en not_active Abandoned
- 2015-12-09 CA CA2970935A patent/CA2970935A1/en active Pending
- 2015-12-09 EP EP15870718.2A patent/EP3234109A4/en active Pending
- 2015-12-09 KR KR1020217022958A patent/KR102519502B1/ko active Active
- 2015-12-09 WO PCT/US2015/064713 patent/WO2016100035A1/en not_active Ceased
- 2015-12-17 AR ARP150104154A patent/AR103080A1/es unknown
- 2015-12-17 TW TW104142402A patent/TW201636421A/zh unknown
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2017
- 2017-06-19 PH PH12017501153A patent/PH12017501153A1/en unknown
- 2017-07-18 ZA ZA2017/04868A patent/ZA201704868B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3234109A1 (en) | 2017-10-25 |
| ZA201704868B (en) | 2019-02-27 |
| KR102281752B1 (ko) | 2021-07-23 |
| BR112017012974A2 (pt) | 2018-01-09 |
| JP6800854B2 (ja) | 2020-12-16 |
| KR102700499B1 (ko) | 2024-08-28 |
| KR20170087520A (ko) | 2017-07-28 |
| AR103080A1 (es) | 2017-04-12 |
| AU2015363008A1 (en) | 2017-06-29 |
| MX2017008176A (es) | 2018-02-09 |
| TW201636421A (zh) | 2016-10-16 |
| CA2970935A1 (en) | 2016-06-23 |
| KR20230050478A (ko) | 2023-04-14 |
| JP2017537649A (ja) | 2017-12-21 |
| US20160215268A1 (en) | 2016-07-28 |
| KR20210094141A (ko) | 2021-07-28 |
| EP3234109A4 (en) | 2018-06-27 |
| KR102519502B1 (ko) | 2023-04-06 |
| CN107250349A (zh) | 2017-10-13 |
| AU2015363008B2 (en) | 2021-12-09 |
| WO2016100035A1 (en) | 2016-06-23 |
| RU2017125728A3 (ru) | 2019-06-03 |
| PH12017501153A1 (en) | 2017-11-27 |
| SG11201704961VA (en) | 2017-07-28 |
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| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
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