RU2017113005A - Композиции, содержащие рекомбинантные клетки bacillus и инсектицид - Google Patents
Композиции, содержащие рекомбинантные клетки bacillus и инсектицид Download PDFInfo
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- RU2017113005A RU2017113005A RU2017113005A RU2017113005A RU2017113005A RU 2017113005 A RU2017113005 A RU 2017113005A RU 2017113005 A RU2017113005 A RU 2017113005A RU 2017113005 A RU2017113005 A RU 2017113005A RU 2017113005 A RU2017113005 A RU 2017113005A
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- 239000000203 mixture Substances 0.000 title claims 25
- 241000193830 Bacillus <bacterium> Species 0.000 title claims 7
- 150000001413 amino acids Chemical group 0.000 claims 18
- 230000008685 targeting Effects 0.000 claims 17
- 102000004169 proteins and genes Human genes 0.000 claims 16
- 108090000623 proteins and genes Proteins 0.000 claims 16
- 108020001507 fusion proteins Proteins 0.000 claims 9
- 102000037865 fusion proteins Human genes 0.000 claims 9
- 241000196324 Embryophyta Species 0.000 claims 8
- 102000004190 Enzymes Human genes 0.000 claims 8
- 108090000790 Enzymes Proteins 0.000 claims 8
- 229940088598 enzyme Drugs 0.000 claims 8
- 230000008635 plant growth Effects 0.000 claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 6
- 230000001737 promoting effect Effects 0.000 claims 6
- 230000015572 biosynthetic process Effects 0.000 claims 5
- 239000002917 insecticide Substances 0.000 claims 5
- 150000004767 nitrides Chemical class 0.000 claims 5
- -1 CYP735A Proteins 0.000 claims 4
- 239000005888 Clothianidin Substances 0.000 claims 4
- PGOOBECODWQEAB-UHFFFAOYSA-N (E)-clothianidin Chemical compound [O-][N+](=O)\N=C(/NC)NCC1=CN=C(Cl)S1 PGOOBECODWQEAB-UHFFFAOYSA-N 0.000 claims 3
- 241000193755 Bacillus cereus Species 0.000 claims 3
- 239000005946 Cypermethrin Substances 0.000 claims 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 3
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- 230000004913 activation Effects 0.000 claims 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 3
- 150000001875 compounds Chemical class 0.000 claims 3
- 229960005424 cypermethrin Drugs 0.000 claims 3
- KAATUXNTWXVJKI-UHFFFAOYSA-N cypermethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OC(C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 KAATUXNTWXVJKI-UHFFFAOYSA-N 0.000 claims 3
- 239000012634 fragment Substances 0.000 claims 3
- 239000008103 glucose Substances 0.000 claims 3
- YFBPRJGDJKVWAH-UHFFFAOYSA-N methiocarb Chemical compound CNC(=O)OC1=CC(C)=C(SC)C(C)=C1 YFBPRJGDJKVWAH-UHFFFAOYSA-N 0.000 claims 3
- 125000004206 2,2,2-trifluoroethyl group Chemical group [H]C([H])(*)C(F)(F)F 0.000 claims 2
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
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- QQODLKZGRKWIFG-RUTXASTPSA-N [(R)-cyano-(4-fluoro-3-phenoxyphenyl)methyl] (1S)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-1-carboxylate Chemical compound CC1(C)C(C=C(Cl)Cl)[C@@H]1C(=O)O[C@@H](C#N)C1=CC=C(F)C(OC=2C=CC=CC=2)=C1 QQODLKZGRKWIFG-RUTXASTPSA-N 0.000 claims 2
- WCXDHFDTOYPNIE-UHFFFAOYSA-N acetamiprid Chemical compound N#CN=C(C)N(C)CC1=CC=C(Cl)N=C1 WCXDHFDTOYPNIE-UHFFFAOYSA-N 0.000 claims 2
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- QGLZXHRNAYXIBU-WEVVVXLNSA-N aldicarb Chemical compound CNC(=O)O\N=C\C(C)(C)SC QGLZXHRNAYXIBU-WEVVVXLNSA-N 0.000 claims 2
- 229960002587 amitraz Drugs 0.000 claims 2
- QXAITBQSYVNQDR-ZIOPAAQOSA-N amitraz Chemical compound C=1C=C(C)C=C(C)C=1/N=C/N(C)\C=N\C1=CC=C(C)C=C1C QXAITBQSYVNQDR-ZIOPAAQOSA-N 0.000 claims 2
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- 229960005286 carbaryl Drugs 0.000 claims 2
- CVXBEEMKQHEXEN-UHFFFAOYSA-N carbaryl Chemical compound C1=CC=C2C(OC(=O)NC)=CC=CC2=C1 CVXBEEMKQHEXEN-UHFFFAOYSA-N 0.000 claims 2
- 229940106157 cellulase Drugs 0.000 claims 2
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- 229960002483 decamethrin Drugs 0.000 claims 2
- OWZREIFADZCYQD-NSHGMRRFSA-N deltamethrin Chemical compound CC1(C)[C@@H](C=C(Br)Br)[C@H]1C(=O)O[C@H](C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 OWZREIFADZCYQD-NSHGMRRFSA-N 0.000 claims 2
- RDYMFSUJUZBWLH-SVWSLYAFSA-N endosulfan Chemical compound C([C@@H]12)OS(=O)OC[C@@H]1[C@]1(Cl)C(Cl)=C(Cl)[C@@]2(Cl)C1(Cl)Cl RDYMFSUJUZBWLH-SVWSLYAFSA-N 0.000 claims 2
- RIZMRRKBZQXFOY-UHFFFAOYSA-N ethion Chemical compound CCOP(=S)(OCC)SCSP(=S)(OCC)OCC RIZMRRKBZQXFOY-UHFFFAOYSA-N 0.000 claims 2
- KVDJTXBXMWJJEF-UHFFFAOYSA-N fluopyram Chemical compound ClC1=CC(C(F)(F)F)=CN=C1CCNC(=O)C1=CC=CC=C1C(F)(F)F KVDJTXBXMWJJEF-UHFFFAOYSA-N 0.000 claims 2
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- WHOOUMGHGSPMGR-UHFFFAOYSA-N indol-3-ylacetaldehyde Chemical compound C1=CC=C2C(CC=O)=CNC2=C1 WHOOUMGHGSPMGR-UHFFFAOYSA-N 0.000 claims 2
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- 238000000034 method Methods 0.000 claims 2
- UHXUZOCRWCRNSJ-QPJJXVBHSA-N methomyl Chemical compound CNC(=O)O\N=C(/C)SC UHXUZOCRWCRNSJ-QPJJXVBHSA-N 0.000 claims 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 2
- RJMUSRYZPJIFPJ-UHFFFAOYSA-N niclosamide Chemical compound OC1=CC=C(Cl)C=C1C(=O)NC1=CC=C([N+]([O-])=O)C=C1Cl RJMUSRYZPJIFPJ-UHFFFAOYSA-N 0.000 claims 2
- 229960001920 niclosamide Drugs 0.000 claims 2
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- 125000003729 nucleotide group Chemical group 0.000 claims 2
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- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 2
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- PAJPWUMXBYXFCZ-UHFFFAOYSA-N 1-aminocyclopropanecarboxylic acid Chemical compound OC(=O)C1(N)CC1 PAJPWUMXBYXFCZ-UHFFFAOYSA-N 0.000 claims 1
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- OOXNYFKPOPJIOT-UHFFFAOYSA-N 5-(3-bromophenyl)-7-(6-morpholin-4-ylpyridin-3-yl)pyrido[2,3-d]pyrimidin-4-amine;dihydrochloride Chemical compound Cl.Cl.C=12C(N)=NC=NC2=NC(C=2C=NC(=CC=2)N2CCOCC2)=CC=1C1=CC=CC(Br)=C1 OOXNYFKPOPJIOT-UHFFFAOYSA-N 0.000 claims 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/52—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing groups, e.g. carboxylic acid amidines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
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Claims (51)
1. Композиция, содержащая:
а) рекомбинантные продуцирующие экзоспорий клетки Bacillus, которые экспрессируют слитый белок, содержащий:
(I) по меньшей мере один белок или пептид, выбранный из группы, включающей стимулирующий рост растений белок или пептид и белок или пептид с инсектицидной активностью; и
(II) нацеливающую последовательность, белок экзоспория, или фрагмент белка экзоспория; и
б) по меньшей мере один инсектицид, выбранный из группы, включающей ацетамиприд, алдикарб, амитраз, бета-цифлутрин, карбарил, клотианидин, цифлутрин, циперметрин, дельтаметрин, эндосульфан, этион, этипрол, этопрофос, фенамифос, фенобукарб, фентион, фипронил, флубендиамид, флуопирам, флупирадифурон, форметанат, гептанофос, имидаклоприд, метамидофос, метиокарб, метомил, никлозамид, оксидеметон-метил, фосалон, силафлуофен, спиродиклофен, спиромезифен, спиротетрамат, тиаклоприд, тиодикарб, тралометрин, триазофос, трифлумурон, вамидотион, 1-{2-фтор-4-метил-5-[(R)-(2,2,2-трифторэтил)сульфинил]фенил}-3-(трифторметил)-1Н-1,2,4-триазол-5-амин, и 1-(3-хлорпиридин-2-ил)-N-[4-циано-2-метил-6-(метилкарбамоил)фенил]-3-{[5-(трифторметил)-2Н-тетразол-2-ил]метил}-1Н-пиразол-5-карбоксамид в синергетически эффективном количестве.
2. Композиция по п. 1, где по меньшей мере один белок или пептид представляет собой стимулирующий рост растений белок или пептид, выбранный из группы, включающей фермент, вовлеченный в продукцию или активацию соединения, стимулирующего рост растений, и фермент, который разлагает или модифицирует бактериальный, грибковый или растительный источник питания.
3. Композиция по п. 1, где продуцирующие экзоспорий клетки Bacillus представляют собой клетки представителя семейства Bacillus cereus.
4. Композиция в соответствии с пунктом 3, где представитель семейства Bacillus cereus выбирают из группы, включающей Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides, Bacillus samanii, Bacillus gaemokensis, Bacillus weihenstephensis, Bacillus toyoiensis и их комбинации.
5. Композиция в соответствии с любым из пп. 1-4, где нацеливающая последовательность или белок экзоспория включает:
аминокислотную последовательность, имеющую по меньшей мере приблизительно 43% идентичность с аминокислотами 20-35 из SEQ ID NO: 1, где идентичность с аминокислотами 25-35 составляет по меньшей мере приблизительно 54%;
нацеливающую последовательность, содержащую аминокислоты 1-35 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 20-35 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 22-31 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 22-33 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 20-31 из SEQ ID NO: 1;
нацеливающая последовательность, содержащая SEQ ID NO: 1; или
белок экзоспория, содержащий аминокислотную последовательность, имеющую по меньшей мере 85% идентичность с SEQ ID NO: 2.
6. Композиция в соответствии с любым из пп. 2-5, где фермент, вовлеченный в продукцию или активацию соединения, стимулирующего рост растений, выбирают из группы, включающей ацетоин-редуктазу, индол-3-ацетамид-гидролазу, триптофан-монооксигеназу, ацетолактат-синтетазу, α-ацетолактат-декарбоксилазу, пируват-декарбоксилазу, диацетил-редуктазу, бутандиол-дегидрогеназу, аминотрансферазу, триптофан-декарбоксилазу, аминоксидазу, индол-3-пируват-декарбоксилазу, индол-3-ацетальдегид дегидрогеназу, оксидазу боковой цепи триптофана, нитрил-гидролазу, нитрилазу, пептидазу, протеазу, аденозинфосфат-изопентенил-трансферазу, фосфатазу, аденозин-киназу, аденин-фосфорибозилтрансферазу, CYP735A, 5'-рибонуклеотид-фосфогидролазу, аденозин-нуклеозидазу, зеатин цис-транс-изомеразу, зеатин О-гликозилтрансферазу, β-глюкозидазу, цис-гидроксилазу, CK цис-гидроксилазу, CK N-гликозилтрансферазу, 2,5-рибонуклеотид фосфогидролазу, аденозин-нуклеозидазу, пуриннуклеозид фосфорилазу, зеатин редуктазу, гидроксиламин редуктазу, 2-оксоглутарат диоксигеназу, гиббереллиновую 2В/3В гидролазу, гиббереллин 3-оксидазу, гиббереллин 20-оксидазу, хитозаназу, хитиназу, β-1,3- глюканазу, β-1,4-глюканазу, β-1,6-глюканазу, дезаминазу аминоциклопропан-1-карбоновой кислоты, и фермент, вовлеченный в продукцию nod-фактора.
7. Композиция по п. 6, где фермент, вовлеченный в продукцию или активацию соединения, стимулирующего рост растений, представляет собой хитозаназу.
8. Композиция по п. 7, где слитый белок включает SEQ ID NO: 109.
9. Композиция в соответствии с любым из пп. 2-5, где фермент, который разлагает или модифицирует бактериальный, грибковый или растительный источник питания, выбирают из группы, включающей целлюлазу, липазу, лигнин-оксидазу, протеазу, гликозид гидролазу, фосфатазу, нитрогеназу, нуклеазу, амидазу, нитрат-редуктазу, нитрит-редуктазу, амилазу, аммоний-оксидазу, лигниназу, глюкозидазу, фосфолипазу, фитазу, пектиназу, глюканазу, сульфатазу, уреазу, ксиланазу, и сидерофор.
10. Композиция по п. 9, где фермент представляет собой целлюлазу, выбранную из группы, включающей эндоцеллюлазу, экзоцеллюлазу и β-глюкозидазу.
11. Композиция по п. 10, где слитый белок включает эндоглюканазу Bacillus subtilis.
12. Композиция по п. 11, где слитый белок включает SEQ ID NO: 107.
13. Композиция по п. 12, где рекомбинантные клетки Bacillus имеют происхождение из Bacillus thuringiensis ВТ013А.
14. Композиция по п. 9, где фермент представляет собой фосфолипазу.
15. Композиция по п. 14, где слитый белок включает SEQ ID NO: 108.
16. Композиция в соответствии с любым из пп. 1-15, где слитый белок экспрессируется под контролем промотора спорообразования, нативного для нацеливающей последовательности, белка экзоспория, или фрагмента белка экзоспория слитого белка.
17. Композиция в соответствии с любым из пп. 1-16, где слитый белок экспрессируется под контролем высокоэкспрессируемого промотора спорообразования.
18. Композиция по п. 17, где высокоэкспрессируемый промотор спорообразования включает промоторную последовательность сигма-K полимеразы, специфической для спорообразования.
19. Композиция в соответствии с любым из пп. 16-18, где промотор спорообразования включает нуклеотидную последовательность, имеющую по меньшей мере 80% идентичность с нуклеотидной последовательностью с любой из SEQ ID NOS: 85-103.
20. Композиция в соответствии с любым из пп. 1-19, где по меньшей мере один инсектицид выбирают из группы, включающей клотианидин, циперметрин, этипрол, фипронил, флупирадифурон, и метиокарб.
21. Композиция по п. 20, где инсектицид представляет собой флупирадифурон.
22. Композиция в соответствии с любым из пп. 1-19, где инсектицид представляет собой клотианидин.
23. Семя, обработанное композицией в соответствии с любым из пп. 1-22.
24. Применение композиции в соответствии с любым из пп. 1-22 для усиления роста растения и/или способствования жизнеспособности растения.
25. Применение по п. 24 для обработки обычных или трансгенных растений или их семян.
26. Способ обработки растения, части растений, или локуса, окружающего растение, для усиления роста растения и/или способствования жизнеспособности растения, включающий стадию одновременного или последовательного использования:
а) рекомбинантных продуцирующих экзоспорий клеток Bacillus, которые экспрессируют слитый белок, содержащий:
(I) по меньшей мере один стимулирующий рост растений белок или пептид; и
(II) нацеливающую последовательность, белок экзоспория, или фрагмент белка экзоспория; и
б) по меньшей мере одного инсектицида, выбранного из группы, включающей ацетамиприд, алдикарб, амитраз, бета-цифлутрин, карбарил, клотианидин, цифлутрин, циперметрин, дельтаметрин, эндосульфан, этион, этипрол, этопрофос, фенамифос, фенобукарб, фентион, фипронил, флубендиамид, флуопирам, флупирадифурон, форметанат, гептанофос, имидаклоприд, метамидофос, метиокарб, метомил, никлозамид, оксидеметон-метил, фосалон, силафлуофен, спиродиклофен, спиромезифен, спиротетрамат, тиаклоприд, тиодикарб, тралометрин, триазофос, трифлумурон, вамидотион, 1-{2-фтор-4-метил-5-[(R)-(2,2,2-трифторэтил)сульфинил]фенил}-3-(трифторметил)-1Н-1,2,4-триазол-5-амин, и 1-(3-хлорпиридин-2-ил)-N-[4-циано-2-метил-6-(метилкарбамоил)фенил]-3-{[5-(трифторметил)-2Н-тетразол-2-ил]метил}-1Н-пиразол-5-карбоксамид в синергетически эффективном количестве.
27. Способ по п. 26, где нацеливающая последовательность или белок экзоспория включает:
аминокислотную последовательность, имеющую по меньшей мере приблизительно 43% идентичность с аминокислотами 20-35 из SEQ ID NO: 1, где идентичность с аминокислотами 25-35 составляет по меньшей мере приблизительно 54%;
нацеливающую последовательность, содержащую аминокислоты 1-35 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 20-35 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 22-31 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 22-33 из SEQ ID NO: 1;
нацеливающую последовательность, содержащую аминокислоты 20-31 из SEQ ID NO: 1;
нацеливающая последовательность, содержащая SEQ ID NO: 1; или
белок экзоспория, содержащий аминокислотную последовательность, имеющую по меньшей мере 85% идентичность с SEQ ID NO: 2.
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