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MX2021011358A - Proteinas de fusion, bacterias recombinantes, y fragmentos de exosporio para el control de plagas y salud vegetal. - Google Patents

Proteinas de fusion, bacterias recombinantes, y fragmentos de exosporio para el control de plagas y salud vegetal.

Info

Publication number
MX2021011358A
MX2021011358A MX2021011358A MX2021011358A MX2021011358A MX 2021011358 A MX2021011358 A MX 2021011358A MX 2021011358 A MX2021011358 A MX 2021011358A MX 2021011358 A MX2021011358 A MX 2021011358A MX 2021011358 A MX2021011358 A MX 2021011358A
Authority
MX
Mexico
Prior art keywords
exosporium
recombinant
fusion proteins
pest control
exosporium fragments
Prior art date
Application number
MX2021011358A
Other languages
English (en)
Inventor
Damian Curtis
Kevin Bugg
Original Assignee
Bayer Cropscience Lp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Cropscience Lp filed Critical Bayer Cropscience Lp
Publication of MX2021011358A publication Critical patent/MX2021011358A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a una proteína de fusión que tiene una secuencia direccionada, proteína de exosporio, o fragmento de proteína de exosporio que dirige la proteína de fusión al exosporio de un miembro de la familia Bacillus cereus recombinante y una enzima que tiene actividad de serina proteasa, en donde la enzima que tiene actividad de serina proteasa es a partir de Bacillus firmus o es una variante de tal enzima. La presente invención también proporciona un miembro de la familia Bacillus cereus recombinante que expresa tal proteína de fusión y fragmentos de exosporio derivados de tal miembro de la familia Bacillus cereus recombinante. También se proporcionan métodos para usar tales miembros de la familia Bacillus cereus recombinantes o fragmentos de exosporio derivados de los mismos para el control de nematodos.
MX2021011358A 2019-03-19 2020-03-18 Proteinas de fusion, bacterias recombinantes, y fragmentos de exosporio para el control de plagas y salud vegetal. MX2021011358A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962820773P 2019-03-19 2019-03-19
PCT/US2020/023255 WO2020190993A1 (en) 2019-03-19 2020-03-18 Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health

Publications (1)

Publication Number Publication Date
MX2021011358A true MX2021011358A (es) 2021-10-13

Family

ID=70293065

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021011358A MX2021011358A (es) 2019-03-19 2020-03-18 Proteinas de fusion, bacterias recombinantes, y fragmentos de exosporio para el control de plagas y salud vegetal.

Country Status (12)

Country Link
US (2) US11793196B2 (es)
EP (1) EP3941204A1 (es)
JP (1) JP2022525639A (es)
KR (1) KR20210142673A (es)
CN (1) CN113825401A (es)
AR (1) AR118443A1 (es)
AU (1) AU2020241392B2 (es)
BR (1) BR112021017827A2 (es)
CA (1) CA3133986A1 (es)
CR (1) CR20210478A (es)
MX (1) MX2021011358A (es)
WO (1) WO2020190993A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3684168A4 (en) 2017-09-20 2021-10-27 Spogen Biotech Inc. FUSION PROTEINS, RECOMBINANT BACTERIA AND EXOSPORIUM FRAGMENTS FOR PLANT HEALTH
AU2020241392B2 (en) * 2019-03-19 2025-05-01 Bayer Cropscience Lp Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health
AR124633A1 (es) * 2020-09-21 2023-04-19 Bayer Cropscience Lp Nuevas serina proteasas
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
WO2023092050A1 (en) * 2021-11-20 2023-05-25 Bayer Cropscience Lp Beneficial combinations with recombinant bacillus cells expressing a serine protease

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GB9518323D0 (en) * 1995-09-07 1995-11-08 Steidler Lothar Materials and methods relating to the attachment and display of substances on cell surfaces
US9133251B2 (en) * 2008-02-22 2015-09-15 The Curators Of The University Of Missouri Bacillus based delivery system and methods of use
US20100112670A1 (en) * 2008-06-25 2010-05-06 Vaxiion Therapeutics, Inc. Regulated genetic suicide mechanism compositions and methods
US20110269804A1 (en) * 2008-08-22 2011-11-03 Syngenta Crop Protection Llc Insecticidal compounds
US20130267415A1 (en) * 2010-12-15 2013-10-10 Syngenta Participations Ag Pesticidal mixtures
DE102012201297A1 (de) 2012-01-31 2013-08-01 Basf Se Expressionsverfahren
EP2809145A4 (en) 2012-02-01 2015-09-16 Dow Agrosciences Llc CHLOROPLAST TRANSIT PEPTIDES
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IL315468A (en) 2014-09-17 2024-11-01 Spogen Biotech Inc Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
AR101957A1 (es) * 2014-09-17 2017-01-25 Bayer Cropscience Lp Composiciones que comprenden células recombinantes de bacillus y un insecticida
US20170295798A1 (en) 2014-09-17 2017-10-19 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and a fungicide
AR101960A1 (es) 2014-09-17 2017-01-25 Bayer Cropscience Lp Composiciones que comprenden células recombinantes de bacillus y un fungicida
CA2961382C (en) 2014-09-17 2024-02-13 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and another biological control agent
EP3193618A1 (en) 2014-09-17 2017-07-26 Bayer Cropscience LP Compositions comprising recombinant bacillus cells and an insecticide
US11154064B2 (en) 2014-09-17 2021-10-26 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and another biological control agent
JP6581346B2 (ja) * 2014-11-19 2019-09-25 花王株式会社 精製アルカリプロテアーゼの製造方法
WO2017161181A1 (en) 2016-03-16 2017-09-21 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and exosporium fragments for animal health and aquaculture
EP3684168A4 (en) 2017-09-20 2021-10-27 Spogen Biotech Inc. FUSION PROTEINS, RECOMBINANT BACTERIA AND EXOSPORIUM FRAGMENTS FOR PLANT HEALTH
AU2020241392B2 (en) * 2019-03-19 2025-05-01 Bayer Cropscience Lp Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health

Also Published As

Publication number Publication date
WO2020190993A1 (en) 2020-09-24
EP3941204A1 (en) 2022-01-26
US20200296960A1 (en) 2020-09-24
BR112021017827A2 (pt) 2021-11-30
JP2022525639A (ja) 2022-05-18
AU2020241392B2 (en) 2025-05-01
US11793196B2 (en) 2023-10-24
AU2020241392A1 (en) 2021-09-30
US20240008485A1 (en) 2024-01-11
CN113825401A (zh) 2021-12-21
CA3133986A1 (en) 2020-09-24
CR20210478A (es) 2021-11-05
US12527323B2 (en) 2026-01-20
KR20210142673A (ko) 2021-11-25
AR118443A1 (es) 2021-10-06

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