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RU2010105710A - Mutant adenylate cyclase, the DNA encoding it, the bacterium of the family Enterobacteriaceae containing the indicated DNA, and the method of obtaining L-amino acids - Google Patents

Mutant adenylate cyclase, the DNA encoding it, the bacterium of the family Enterobacteriaceae containing the indicated DNA, and the method of obtaining L-amino acids Download PDF

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RU2010105710A
RU2010105710A RU2010105710/10A RU2010105710A RU2010105710A RU 2010105710 A RU2010105710 A RU 2010105710A RU 2010105710/10 A RU2010105710/10 A RU 2010105710/10A RU 2010105710 A RU2010105710 A RU 2010105710A RU 2010105710 A RU2010105710 A RU 2010105710A
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bacterium
amino acid
adenylate cyclase
aromatic
group
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RU2010105710/10A
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RU2471868C2 (en
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Наталья Сергеевна Ерёмина (RU)
Наталья Сергеевна Ерёмина
Наталия Викторовна Стойнова (RU)
Наталия Викторовна Стойнова
Татьяна Абрамовна Ямпольская (RU)
Татьяна Абрамовна Ямпольская
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Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU)
Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ)
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Priority to RU2010105710/10A priority Critical patent/RU2471868C2/en
Priority to PCT/JP2011/052967 priority patent/WO2011102305A2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y406/00—Phosphorus-oxygen lyases (4.6)
    • C12Y406/01—Phosphorus-oxygen lyases (4.6.1)
    • C12Y406/01001—Aodenylate cyclase (4.6.1.1)

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Abstract

1. Мутантная аденилатциклаза, выбранная из группы, состоящей из: ! (A) белка, в котором L-лизин в положении, соответствующем положению 432 в SEQ ID NO: 2, замещен другим аминокислотным остатком; и ! (B) варианта белка (А), обладающего аденилатциклазной активностью. ! 2. Мутантная аденилатциклаза по п.1, отличающаяся тем, что L-лизин в положении, соответствующем положению 432 в SEQ ID NO: 2, замещен L-глутамином. ! 3. ДНК, кодирующая аденилатциклазу по любому из пп.1 и 2. ! 4. Бактерия семейства Enterobacteriaceae, содержащая ДНК по п.3. ! 5. Бактерия по п.4, отличающаяся тем, что указанная бактерия способна к продукции L-аминокислот. ! 6. Бактерия по п.4, отличающаяся тем, что указанная бактерия принадлежит к роду Escherichia. ! 7. Бактерия по п.6, отличающаяся тем, что указанной бактерией является бактерия Escherichia coli. ! 8. Способ получения L-аминокислоты, включающий выращивание бактерии по любому из пп.5-7 в питательной среде, содержащей в качестве единственного источника углерода этанол или глицерин, и выделение указанной L-аминокислоты из культуральной жидкости. ! 9. Способ по п.8, отличающийся тем, что указанная L-аминокислота выбрана из группы, состоящей из ароматической L-аминокислоты и неароматической L-аминокислоты. ! 10. Способ по п.9, отличающийся тем, что указанная ароматическая L-аминокислота выбрана из группы, состоящей из L-фенилаланина, L-тирозина и L-триптофана. ! 11. Способ по п.9, отличающийся тем, что указанная неароматическая L-аминокислота выбрана из группы, состоящей из L-треонина, L-лизина, L-цистеина, L-метионина, L-лейцина, L-изолейцина, L-валина, L-гистидина, L-глицина, L-серина, L-аланина, L-аспарагина, L-аспартата, L-глутамина, L-глутаминовой кислоты, L-пролина и L-аргини 1. Mutant adenylate cyclase selected from the group consisting of:! (A) a protein in which L-lysine at the position corresponding to position 432 in SEQ ID NO: 2 is substituted with another amino acid residue; and! (B) a variant of the protein (A) having adenylate cyclase activity. ! 2. The mutant adenylate cyclase according to claim 1, characterized in that L-lysine at the position corresponding to position 432 in SEQ ID NO: 2 is substituted with L-glutamine. ! 3. DNA encoding adenylate cyclase according to any one of claims 1 and 2.! 4. A bacterium of the Enterobacteriaceae family containing DNA according to claim 3. ! 5. The bacterium according to claim 4, characterized in that said bacterium is capable of producing L-amino acids. ! 6. The bacterium according to claim 4, characterized in that said bacterium belongs to the genus Escherichia. ! 7. The bacterium according to claim 6, characterized in that said bacterium is Escherichia coli. ! 8. A method for producing an L-amino acid, comprising growing the bacterium according to any one of claims 5 to 7 in a nutrient medium containing ethanol or glycerol as the sole carbon source, and isolating said L-amino acid from the culture fluid. ! 9. The method of claim 8, wherein said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. ! 10. The method according to claim 9, characterized in that said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tyrosine and L-tryptophan. ! 11. The method according to claim 9, characterized in that said non-aromatic L-amino acid is selected from the group consisting of L-threonine, L-lysine, L-cysteine, L-methionine, L-leucine, L-isoleucine, L-valine , L-histidine, L-glycine, L-serine, L-alanine, L-asparagine, L-aspartate, L-glutamine, L-glutamic acid, L-proline and L-arginine

Claims (11)

1. Мутантная аденилатциклаза, выбранная из группы, состоящей из:1. Mutant adenylate cyclase selected from the group consisting of: (A) белка, в котором L-лизин в положении, соответствующем положению 432 в SEQ ID NO: 2, замещен другим аминокислотным остатком; и(A) a protein in which L-lysine at the position corresponding to position 432 in SEQ ID NO: 2 is substituted with another amino acid residue; and (B) варианта белка (А), обладающего аденилатциклазной активностью.(B) a variant of the protein (A) having adenylate cyclase activity. 2. Мутантная аденилатциклаза по п.1, отличающаяся тем, что L-лизин в положении, соответствующем положению 432 в SEQ ID NO: 2, замещен L-глутамином.2. The mutant adenylate cyclase according to claim 1, characterized in that L-lysine at the position corresponding to position 432 in SEQ ID NO: 2 is substituted with L-glutamine. 3. ДНК, кодирующая аденилатциклазу по любому из пп.1 и 2.3. DNA encoding adenylate cyclase according to any one of claims 1 and 2. 4. Бактерия семейства Enterobacteriaceae, содержащая ДНК по п.3.4. A bacterium of the Enterobacteriaceae family containing DNA according to claim 3. 5. Бактерия по п.4, отличающаяся тем, что указанная бактерия способна к продукции L-аминокислот.5. The bacterium according to claim 4, characterized in that said bacterium is capable of producing L-amino acids. 6. Бактерия по п.4, отличающаяся тем, что указанная бактерия принадлежит к роду Escherichia.6. The bacterium according to claim 4, characterized in that said bacterium belongs to the genus Escherichia. 7. Бактерия по п.6, отличающаяся тем, что указанной бактерией является бактерия Escherichia coli.7. The bacterium according to claim 6, characterized in that said bacterium is Escherichia coli. 8. Способ получения L-аминокислоты, включающий выращивание бактерии по любому из пп.5-7 в питательной среде, содержащей в качестве единственного источника углерода этанол или глицерин, и выделение указанной L-аминокислоты из культуральной жидкости.8. A method for producing an L-amino acid, comprising growing a bacterium according to any one of claims 5-7 in a nutrient medium containing ethanol or glycerol as the sole carbon source, and isolating said L-amino acid from the culture fluid. 9. Способ по п.8, отличающийся тем, что указанная L-аминокислота выбрана из группы, состоящей из ароматической L-аминокислоты и неароматической L-аминокислоты.9. The method of claim 8, wherein said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. 10. Способ по п.9, отличающийся тем, что указанная ароматическая L-аминокислота выбрана из группы, состоящей из L-фенилаланина, L-тирозина и L-триптофана.10. The method according to claim 9, characterized in that said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tyrosine and L-tryptophan. 11. Способ по п.9, отличающийся тем, что указанная неароматическая L-аминокислота выбрана из группы, состоящей из L-треонина, L-лизина, L-цистеина, L-метионина, L-лейцина, L-изолейцина, L-валина, L-гистидина, L-глицина, L-серина, L-аланина, L-аспарагина, L-аспартата, L-глутамина, L-глутаминовой кислоты, L-пролина и L-аргинина. 11. The method according to claim 9, characterized in that said non-aromatic L-amino acid is selected from the group consisting of L-threonine, L-lysine, L-cysteine, L-methionine, L-leucine, L-isoleucine, L-valine , L-histidine, L-glycine, L-serine, L-alanine, L-asparagine, L-aspartate, L-glutamine, L-glutamic acid, L-proline and L-arginine.
RU2010105710/10A 2010-02-18 2010-02-18 Mutant adenylate cyclase, dna coding it, bacteria of enterobacteriaceae family containing said dan and method for preparing l-amino acids RU2471868C2 (en)

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RU2010105710/10A RU2471868C2 (en) 2010-02-18 2010-02-18 Mutant adenylate cyclase, dna coding it, bacteria of enterobacteriaceae family containing said dan and method for preparing l-amino acids
PCT/JP2011/052967 WO2011102305A2 (en) 2010-02-18 2011-02-04 A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE Enterobacteriaceae FAMILY HAVING A MUTANT ADENYLATE CYCLASE

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101996767B1 (en) * 2018-11-29 2019-07-04 씨제이제일제당 (주) cAMP receptor protein variant and method for producing L-amino acid using the same
KR101991206B1 (en) * 2018-11-29 2019-06-19 씨제이제일제당 (주) cAMP receptor protein variant and method for producing L-amino acid using the same
JP7793519B2 (en) 2019-12-19 2026-01-05 ビーエーエスエフ ソシエタス・ヨーロピア Increased space-time yield, carbon conversion efficiency, and carbon substrate adaptability in producing fine chemicals
EP4370653A2 (en) 2021-07-16 2024-05-22 Biosyntia ApS Microbial cell factories producing vitamin b compounds

Family Cites Families (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR356739A (en) 1904-09-20 1905-12-07 Charles Glauser Perrin Winding and time-setting mechanism
SU875663A1 (en) 1978-06-30 1982-09-15 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Strains e. coli vniigenetika voltage 334 ru no.6 and vniigenetika voltage 334 no.7 producersof l-treonite and method for preparing them
JPS561890A (en) 1979-06-15 1981-01-10 Ajinomoto Co Inc Preparation of l-phenylalanine by fermentation
JPS565099A (en) 1979-06-25 1981-01-20 Ajinomoto Co Inc Production of l-histidine through fermentation process and microorganism used therefor
JPS5618596A (en) 1979-07-23 1981-02-21 Ajinomoto Co Inc Production of l-lysine through fermentation process
JPS5672695A (en) 1979-11-15 1981-06-16 Ajinomoto Co Inc Preparation of l-leucine
JPS5685289A (en) 1979-12-13 1981-07-11 Ajinomoto Co Inc Preparation of l-valine by fermentation
JPS56144093A (en) 1980-04-14 1981-11-10 Ajinomoto Co Inc Preparation of l-proline by fermentation
US4371614A (en) 1980-08-22 1983-02-01 Ajinomoto Co., Inc. E.Coli bacteria carrying recombinant plasmids and their use in the fermentative production of L-tryptophan
DE3127361A1 (en) 1981-07-08 1983-02-03 Schering Ag, 1000 Berlin Und 4619 Bergkamen PRODUCTION AND APPLICATION OF PLASMIDES WITH GENES FOR THE BIOSYNTHESIS OF L-PROLIN
DE3565475D1 (en) 1984-04-07 1988-11-17 Bayer Ag Process and apparatus for the production of granules
JPH06102024B2 (en) 1986-04-16 1994-12-14 味の素株式会社 Novel promoter and gene expression method using the promoter
FR2603581B1 (en) 1986-04-28 1993-08-13 Ajinomoto Kk PROCESS FOR ISOLATING AND PURIFYING AMINO ACIDS BY CHROMATOGRAPHY
US4777051A (en) 1986-06-20 1988-10-11 Ajinomoto Co., Inc. Process for the production of a composition for animal feed
KR890003681B1 (en) 1987-03-26 1989-09-30 주식회사 미원 Process for preparing l-phenyl-alanine by microorganism
JP2536570B2 (en) 1987-10-12 1996-09-18 味の素株式会社 Fermentation method for producing L-isoleucine
JP2537260B2 (en) 1988-02-23 1996-09-25 東陶機器株式会社 Japanese-style toilet construction method
WO1990004636A1 (en) 1988-10-25 1990-05-03 Vsesojuzny Nauchno-Issledovatelsky Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov (Vniigenetika) Strain of bacteria escherichia coli, producer of l-threonine
US5705371A (en) 1990-06-12 1998-01-06 Ajinomoto Co., Inc. Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine
JPH07108228B2 (en) 1990-10-15 1995-11-22 味の素株式会社 Temperature sensitive plasmid
EP1270721B1 (en) 1990-11-30 2007-11-21 Ajinomoto Co., Inc. Recombinant DNA sequences encoding feedback inhibition released enzymes, plasmids comprising the recombinant DNA sequences, transformed microorganisms useful in the production of aromatic amino acids, and a process for preparing aromatic amino acids by fermentation
US5534421A (en) 1991-05-30 1996-07-09 Ajinomoto Co., Inc. Production of isoleucine by escherichia coli having isoleucine auxotrophy and no negative feedback inhibition of isoleucine production
BR9203053A (en) 1991-08-07 1993-03-30 Ajinomoto Kk PROCESS TO PRODUCE L-GLUTAMIC ACID PRO FERMENTATION
JP3006926B2 (en) 1991-09-04 2000-02-07 協和醗酵工業株式会社 Method for producing L-threonine by fermentation method
DE4130868C2 (en) 1991-09-17 1994-10-13 Degussa Animal feed supplement based on an amino acid and process for its preparation
JP3036930B2 (en) 1991-11-11 2000-04-24 協和醗酵工業株式会社 Production method of L-isoleucine by fermentation method
JP3151073B2 (en) 1992-02-25 2001-04-03 協和醗酵工業株式会社 Production of amino acids by fermentation
RU2003677C1 (en) 1992-03-30 1993-11-30 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Strain of bacterium escherichia coli - a producer of l-histidine
DE4232468A1 (en) 1992-09-28 1994-03-31 Consortium Elektrochem Ind Microorganisms for the production of tryptophan and process for their production
EP0593792B2 (en) 1992-10-14 2004-01-07 Ajinomoto Co., Inc. Novel L-threonine-producing microbacteria and a method for the production of L-threonine
SK279915B6 (en) 1992-11-10 1999-05-07 Ajinomoto Co. Dna coding aspartokinase iii, transformed microorganism, and process for producing l-threonine
US5354672A (en) 1992-11-24 1994-10-11 Ian Fotheringham Materials and methods for hypersecretion of amino acids
JP2817400B2 (en) 1993-10-28 1998-10-30 味の素株式会社 Method of manufacturing substances
JPH07155184A (en) 1993-12-08 1995-06-20 Ajinomoto Co Inc Production of l-lysine by fermentation method
JP3880636B2 (en) 1994-01-10 2007-02-14 味の素株式会社 Method for producing L-glutamic acid by fermentation
US5998178A (en) 1994-05-30 1999-12-07 Ajinomoto Co., Ltd. L-isoleucine-producing bacterium and method for preparing L-isoleucine through fermentation
JP3698758B2 (en) 1994-06-30 2005-09-21 協和醗酵工業株式会社 Method for producing L-leucine by fermentation
CN1124340C (en) 1994-08-30 2003-10-15 味之素株式会社 Process for producing L-valine and L-leucine
MX9704236A (en) 1994-12-09 1998-01-31 Ajinomoto Kk Novel lysine decarboxylase gene and process for producing l-lysine.
CN1193343A (en) 1995-08-23 1998-09-16 味之素株式会社 Process for producing L-glutamic acid by fermentation method
JP4032441B2 (en) 1995-08-30 2008-01-16 味の素株式会社 Method for producing L-amino acid
GB2304718B (en) 1995-09-05 2000-01-19 Degussa The production of tryptophan by the bacterium escherichia coli
DE19539952A1 (en) 1995-10-26 1997-04-30 Consortium Elektrochem Ind Process for the preparation of O-acetylserine, L-cysteine and L-cysteine-related products
JPH09285294A (en) 1996-04-23 1997-11-04 Ajinomoto Co Inc Production of l-glutamic acid by fermentation
DE19621930C1 (en) 1996-05-31 1997-12-11 Degussa Process for the preparation of an animal feed additive based on fermentation broth
US5939307A (en) 1996-07-30 1999-08-17 The Archer-Daniels-Midland Company Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production
RU2119536C1 (en) 1997-01-21 1998-09-27 Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Strain escherichia coli - a producer of l-histidine
DE19726083A1 (en) 1997-06-19 1998-12-24 Consortium Elektrochem Ind Microorganisms and processes for the fermentative production of L-cysteine, L-cystine, N-acetyl-serine or thiazolidine derivatives
RU2140450C1 (en) 1997-10-29 1999-10-27 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ") Strains of bacterium escherichia coli - producers of l-leucine (variants)
JP4151094B2 (en) 1997-11-25 2008-09-17 味の素株式会社 Method for producing L-cysteine
AU756507B2 (en) 1998-03-18 2003-01-16 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
AU746542B2 (en) 1998-03-18 2002-05-02 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
JP4294123B2 (en) 1998-07-03 2009-07-08 協和発酵バイオ株式会社 Method for producing metabolites biosynthesized via phosphoribosyl pyrophosphate
US6403358B1 (en) * 1999-10-05 2002-06-11 Millennium Pharmaceuticals, Inc. 21529, a novel adenylate cyclase
JP4110641B2 (en) 1998-11-17 2008-07-02 味の素株式会社 Method for producing L-methionine by fermentation
RU2148642C1 (en) 1998-12-23 2000-05-10 ЗАО "Научно-исследовательский институт АДЖИНОМОТО-Генетика" (ЗАО "АГРИ") Dna rhtc fragment encoding synthesis of rhtc protein that determines enhanced resistance of bacterium escherichia coli to l-threonine and method of l-amino acid producing
RU2175351C2 (en) 1998-12-30 2001-10-27 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ") Escherichia coli dna fragment determining enhanced production of l-amino acids (variants) and method of l-amino acid producing
JP2000262288A (en) 1999-03-16 2000-09-26 Ajinomoto Co Inc Temperature-sensitive plasmid of coryneform bacterium
US6238714B1 (en) 1999-05-05 2001-05-29 Degussa-Huls Ag Feedstuff additive which contains D-pantothenic acid and/or its salts and a process for the preparation thereof
RU2201454C2 (en) 1999-07-09 2003-03-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Mutant alpha-isopropylmalate synthase (ipms), dna encoding mutant ipms, method of preparing escherichia coli strain, method of l-leucine preparing
JP4427878B2 (en) 1999-08-20 2010-03-10 味の素株式会社 Method for producing L-glutamic acid by fermentation method with precipitation
CA2319283A1 (en) 1999-09-20 2001-03-20 Kuniki Kino Method for producing l-amino acids by fermentation
JP4245746B2 (en) 1999-09-20 2009-04-02 協和発酵バイオ株式会社 Amino acid production by fermentation
DE19949579C1 (en) 1999-10-14 2000-11-16 Consortium Elektrochem Ind Microorganism with deregulated cysteine metabolism, useful for high-level production of cysteine and its derivatives, has increased activity of the CysB transcription regulator
BRPI0016995B1 (en) 2000-01-21 2016-03-08 Ajinomoto Kk genetically modified escherichia coli bacteria, and method for producing l-lysine
RU2212447C2 (en) 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Strain escherichia coli as producer of amino acid (variants) and method for preparing amino acid (variants)
RU2215783C2 (en) 2001-05-15 2003-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото - Генетика" Mutant n-acetylglutamate synthase (variants), dna fragment, strain of microorganism escherichia coli as producer of arginine and method for preparing l-arginine
JP4682454B2 (en) 2000-06-28 2011-05-11 味の素株式会社 Process for producing novel mutant N-acetylglutamate synthase and L-arginine
JP4380029B2 (en) 2000-07-05 2009-12-09 味の素株式会社 Manufacturing method of substances using microorganisms
RU2207371C2 (en) 2000-09-26 2003-06-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Method for preparing l-amino acids of l-glutamic acid family, strain of bacterium escherichia coli as producer of l-amino acid (variants)
EP1172433B1 (en) 2000-07-06 2006-06-14 Ajinomoto Co., Inc. Bacterium having ability to produce L-glutamic acid, L-proline or L-arginine and method for producing L-glutamic acid, L-proline or L-arginine
RU2208640C2 (en) 2000-07-06 2003-07-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Methof for preparing l-arginine, strain escherichia coli as producer of l-arginine
RU2215785C2 (en) * 2001-06-28 2003-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acid (variants)
BR0200350B1 (en) 2001-02-13 2014-10-14 Ajinomoto Kk Transgenic bacterium producing L-amino acid belonging to the genus Escherichia and method for producing L-amino acid
RU2229513C2 (en) 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acids (variants)
MXPA04004874A (en) 2001-11-23 2004-09-03 Ajinomoto Kk Process for producing l-amino acid using escherichia.
KR100459758B1 (en) 2002-05-15 2004-12-03 씨제이 주식회사 A nucleotide sequence of threonine operon deregulated from isoleucine and a method for producing L-threonine using a transformed cell containing the same
EP1489092A1 (en) * 2003-06-18 2004-12-22 Institut Pasteur Modified Bordetella adenylate cyclase comprising or lacking CD11b/CD18 interaction domain and uses thereof
DE10331366A1 (en) 2003-07-11 2005-01-27 Degussa Ag Granulating feed additives containing amino acids and/or vitamins, especially L-threonine, optionally in fermentation broth form, using circulating fluidized bed
RU2003121601A (en) 2003-07-16 2005-02-27 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ") (RU) MUTANT SERINACETHYL TRANSFERASE
FR2857671B1 (en) 2003-07-18 2007-10-05 Centre Nat Rech Scient NOVEL METHOD FOR CULTURING KERATINOCYTES AND ITS APPLICATIONS
WO2005010794A2 (en) 2003-07-29 2005-02-03 Ajinomoto Co., Inc. Method for determining metabolic flux affecting substance production
JP4380305B2 (en) 2003-11-21 2009-12-09 味の素株式会社 Method for producing L-amino acid by fermentation
RU2316588C1 (en) 2004-01-30 2008-02-10 Адзиномото Ко., Инк. Microorganism as producer of l-amino acid and method for preparing l-amino acid (variants)
WO2005103275A1 (en) 2004-04-26 2005-11-03 Ajinomoto Co., Ltd. Process for producing l-tryptophan according to fermentation process
US7422880B2 (en) * 2005-01-19 2008-09-09 Ajinomoto Co., Inc. Method for producing an l-amino acid using a bacterium of the enterobacteriaceae family having a pathway of glycogen biosynthesis disrupted
DE102005018835A1 (en) 2005-04-22 2006-11-02 Degussa Ag Process for the preparation of L-amino acids using improved strains of the family Enterobacteriaceae
DE102005019040A1 (en) 2005-04-23 2006-10-26 Degussa Ag Process for the preparation of L-amino acids using improved strains of the family Enterobacteriaceae
CN101490251B (en) 2006-07-19 2016-06-29 味之素株式会社 Method for producing L-amino acid using Enterobacteriaceae bacteria
RU2006145712A (en) 2006-12-22 2008-06-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) METHOD FOR PRODUCING L-AMINO ACIDS BY THE FERMENTATION METHOD USING BACTERIA HAVING AN INCREASED ABILITY FOR GYLICERINE DISPOSAL
JP2010110217A (en) 2007-02-22 2010-05-20 Ajinomoto Co Inc L-amino acid-producing microorganism and method for producing l-amino acid
DE102007051024A1 (en) 2007-03-05 2008-09-11 Evonik Degussa Gmbh Process for the preparation of L-amino acids using strains of the family Enterobacteriaceae
US8624402B2 (en) 2008-03-26 2014-01-07 Stats Chippac Ltd Mock bump system for flip chip integrated circuits

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