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RU2003121601A - MUTANT SERINACETHYL TRANSFERASE - Google Patents

MUTANT SERINACETHYL TRANSFERASE Download PDF

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Publication number
RU2003121601A
RU2003121601A RU2003121601/13A RU2003121601A RU2003121601A RU 2003121601 A RU2003121601 A RU 2003121601A RU 2003121601/13 A RU2003121601/13 A RU 2003121601/13A RU 2003121601 A RU2003121601 A RU 2003121601A RU 2003121601 A RU2003121601 A RU 2003121601A
Authority
RU
Russia
Prior art keywords
serine acetyltransferase
cysteine
mutant
positions
weakened
Prior art date
Application number
RU2003121601/13A
Other languages
Russian (ru)
Inventor
Татсуки КАСИВАГИ (JP)
Татсуки КАСИВАГИ
Юко КАИ (JP)
Юко КАИ
Коки ИСИКАВА (JP)
Коки ИСИКАВА
Эи-ичиро Сузуки (JP)
Эи-ичиро Сузуки
Хироси ТАКАГИ (JP)
Хироси ТАКАГИ
тдинов Михаил Харисович Зи (RU)
Михаил Харисович Зиятдинов
Екатерина Игоревна Редькина (RU)
Екатерина Игоревна Редькина
тинер Михаил Маркович Гус (RU)
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ") (RU)
Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ")
Аджиномото Ко., Инк. (JP)
Аджиномото Ко., Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ") (RU), Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ"), Аджиномото Ко., Инк. (JP), Аджиномото Ко., Инк. filed Critical Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО "АГРИ") (RU)
Priority to RU2003121601/13A priority Critical patent/RU2003121601A/en
Priority to AT04747681T priority patent/ATE554162T1/en
Priority to CNB2004800202648A priority patent/CN100471949C/en
Priority to PCT/JP2004/010216 priority patent/WO2005007841A1/en
Priority to JP2005511865A priority patent/JP4923573B2/en
Priority to EP04747681A priority patent/EP1650296B1/en
Priority to BRPI0412535-5A priority patent/BRPI0412535A/en
Priority to US10/892,112 priority patent/US7312058B2/en
Publication of RU2003121601A publication Critical patent/RU2003121601A/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (7)

1. Мутантная серинацетилтрансфераза, отличающаяся тем, что последовательность аминокислот, соответствующая положениям с 89 по 96 в последовательности природной серинацетилтрансферазы, содержит одну или несколько мутаций, и в которой ингибирование L-цистеином по принципу обратной связи ослаблено.1. Mutant serine acetyltransferase, characterized in that the amino acid sequence corresponding to positions 89 to 96 in the sequence of natural serine acetyltransferase contains one or more mutations, and in which the inhibition of L-cysteine by the feedback principle is weakened. 2. Мутантная серинацетилтрансфераза по п.1, отличающаяся тем, что последовательность аминокислот, соответствующая положениям с 89 по 96 в последовательности природной серинацетилтрансферазы, заменена любой из последовательностей аминокислот SEQ ID NO:3 - SEQ ID NO:8, и ингибирование L-цистеином по принципу обратной связи ослаблено.2. The mutant serine acetyltransferase according to claim 1, characterized in that the amino acid sequence corresponding to positions 89 to 96 in the sequence of the natural serine acetyltransferase is replaced by any of the amino acid sequences of SEQ ID NO: 3 to SEQ ID NO: 8, and the inhibition of L-cysteine by feedback principle is weakened. 3. Мутантная серинацетилтрансфераза по п.1, или 2, отличающаяся тем, что природной сериновой ацетилтрансферазой является серинацетилтрансфераза из Escherichia coli.3. The mutant serine acetyltransferase according to claim 1, or 2, characterized in that the natural serine acetyltransferase is serine acetyltransferase from Escherichia coli. 4. Мутантная серинацетилтрансфераза по любому из пп.1-3, отличающаяся тем, что такая серинацетилтрансфераза включает делеции, замены, вставки или добавления одной или нескольких аминокислот в одном или нескольких положениях, кроме положений с 89 по 96, и исключая тройной мутант с заменами Asn на Lys в позиции 51, Arg на His в позиции 91 и His на Tyr в позиции 233, и ингибирование L-цистеином по принципу обратной связи ослаблено.4. The mutant serine acetyltransferase according to any one of claims 1 to 3, characterized in that such serine acetyltransferase includes deletions, substitutions, insertions or additions of one or more amino acids in one or more positions, except positions 89 to 96, and excluding the triple mutant with substitutions Asn at Lys at position 51, Arg at His at position 91, and His at Tyr at position 233, and feedback inhibition of L-cysteine is weakened. 5. ДНК, кодирующая мутантную сериновую ацетилтрансферазу по любому из пп.1-4.5. DNA encoding the mutant serine acetyltransferase according to any one of claims 1 to 4. 6. Бактерия, принадлежащая к роду Escherichia, отличающаяся тем, что такая бактерия трансформирована с помощью ДНК по п.5, и обладает способностью к продукции L-цистеина.6. A bacterium belonging to the genus Escherichia, characterized in that such a bacterium is transformed using DNA according to claim 5, and has the ability to produce L-cysteine. 7. Способ продукции L-цистеина, включающий стадии выращивания бактерии по п.6 в питательной среде с целью продукции и накопления L-цистеина в питательной среде, и выделения L-цистеина из культуральной жидкости.7. A method for producing L-cysteine, comprising the steps of growing a bacterium according to claim 6 in a nutrient medium for the purpose of producing and accumulating L-cysteine in a nutrient medium, and isolating L-cysteine from the culture fluid.
RU2003121601/13A 2003-07-16 2003-07-16 MUTANT SERINACETHYL TRANSFERASE RU2003121601A (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
RU2003121601/13A RU2003121601A (en) 2003-07-16 2003-07-16 MUTANT SERINACETHYL TRANSFERASE
AT04747681T ATE554162T1 (en) 2003-07-16 2004-07-16 MUTANT SERINE ACETYL TRANSFERASE AND METHOD FOR PRODUCING L-CYSTEIN
CNB2004800202648A CN100471949C (en) 2003-07-16 2004-07-16 Serine acetyltransferase mutant and method for producing L-cysteine
PCT/JP2004/010216 WO2005007841A1 (en) 2003-07-16 2004-07-16 Variant serine acetyltransferase and process for producing l-cysteine
JP2005511865A JP4923573B2 (en) 2003-07-16 2004-07-16 Method for producing mutant serine acetyltransferase and L-cysteine
EP04747681A EP1650296B1 (en) 2003-07-16 2004-07-16 Mutant serine acetyltransferase and process for producing l-cysteine
BRPI0412535-5A BRPI0412535A (en) 2003-07-16 2004-07-16 mutant serine acetyltransferase, DNA, bacterium, and method for producing l-cysteine
US10/892,112 US7312058B2 (en) 2003-07-16 2004-07-16 Mutant serine acetyltransferase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2003121601/13A RU2003121601A (en) 2003-07-16 2003-07-16 MUTANT SERINACETHYL TRANSFERASE

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2003135291/13A Substitution RU2279477C2 (en) 2003-07-16 2003-12-05 Mutant serineacetyl transferase, dna fragment encoding mutant serineacetyl transferase (variants), bacteria belonging to genus escherichia as l-cysteine producer and method for production of l-cysteine

Publications (1)

Publication Number Publication Date
RU2003121601A true RU2003121601A (en) 2005-02-27

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ID=35285774

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2003121601/13A RU2003121601A (en) 2003-07-16 2003-07-16 MUTANT SERINACETHYL TRANSFERASE

Country Status (2)

Country Link
CN (1) CN100471949C (en)
RU (1) RU2003121601A (en)

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007100009A1 (en) 2006-03-03 2007-09-07 Ajinomoto Co., Inc. Method for production of l-amino acid
WO2007125954A1 (en) 2006-04-28 2007-11-08 Ajinomoto Co., Inc. Microorganism capable of producing l-amino acid, and process for production of l-amino acid
WO2008090770A1 (en) 2007-01-22 2008-07-31 Ajinomoto Co., Inc. Microorganism capable of producing l-amino acid, and method for production of l-amino acid
WO2008102572A1 (en) 2007-02-20 2008-08-28 Ajinomoto Co., Inc. Method for production of l-amino acid or nucleic acid
WO2008102861A1 (en) 2007-02-22 2008-08-28 Ajinomoto Co., Inc. Method of producing l-amino acid
EP2055771A2 (en) 2006-03-23 2009-05-06 Ajinomoto Co., Inc. A method for producing an L-amino acid using bacterium of the Enterobacteriaceae family with attenuated expression of a gene coding for small RNA
WO2009088049A1 (en) 2008-01-10 2009-07-16 Ajinomoto Co., Inc. Method for production of desired substance by fermentation process
WO2009093703A1 (en) 2008-01-23 2009-07-30 Ajinomoto Co., Inc. Method of producing l-amino acid
EP2093291A1 (en) 2008-02-19 2009-08-26 Ajinomoto Co., Inc. A method for constructing an operon containing translationally coupled genes
WO2010027045A1 (en) 2008-09-08 2010-03-11 味の素株式会社 Microorganism capable of producing l-amino acid, and method for producing l-amino acid
WO2010084995A2 (en) 2009-01-23 2010-07-29 Ajinomoto Co.,Inc. A method for producing an l-amino acid
WO2011013707A1 (en) 2009-07-29 2011-02-03 味の素株式会社 Method for producing l-amino acid
WO2011043485A1 (en) 2009-10-05 2011-04-14 Ajinomoto Co.,Inc. A METHOD FOR PRODUCING AN L-CYSTEINE, L-CYSTINE, A DERIVATIVE OR PRECURSOR THEREOF OR A MIXTURE THEREOF USING A BACTERIUM OF Enterobacteriaceae FAMILY
EP2345667A2 (en) 2010-01-15 2011-07-20 Ajinomoto Co., Inc. A method for producing an L-amino acid using a bacterium of the enterobacteriaceae family
WO2011096554A1 (en) 2010-02-08 2011-08-11 味の素株式会社 MANUFACTURING METHOD FOR MUTANT rpsA GENE AND L-AMINO ACID
WO2011102305A2 (en) 2010-02-18 2011-08-25 Ajinomoto Co.,Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE Enterobacteriaceae FAMILY HAVING A MUTANT ADENYLATE CYCLASE
WO2012011596A1 (en) 2010-07-21 2012-01-26 Ajinomoto Co.,Inc. A method for producing an l- amino acid using a bacterium of the enterobacteriaceae family with enhanced expression of the bssr gene
WO2012011595A1 (en) 2010-07-21 2012-01-26 Ajinomoto Co.,Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY HAVING ATTENUATED EXPRESSION OF THE astCADBE OPERON
EP2460873A1 (en) 2006-12-12 2012-06-06 Ajinomoto Co., Inc. A method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family with attenuated expression of any of the cynT, cynS, cynX or cynR genes or a combination thereof
WO2012077739A1 (en) 2010-12-10 2012-06-14 味の素株式会社 Method for producing l-amino acid
EP2559754A2 (en) 2011-08-18 2013-02-20 Ajinomoto Co., Inc. Method for producing an L-amino acid using a bacterium of the family enterobacteriaceae having enhanced expression of the flagella formation and motility cascade genes
WO2015005406A1 (en) 2013-07-09 2015-01-15 味の素株式会社 Method for manufacturing useful substance
WO2015050234A1 (en) 2013-10-02 2015-04-09 味の素株式会社 Ammonia control apparatus and ammonia control method
WO2015060314A1 (en) 2013-10-21 2015-04-30 味の素株式会社 Method for producing l-amino acid
EP3385389A1 (en) 2017-04-03 2018-10-10 Ajinomoto Co., Inc. Method for producing l-amino acid from fructose
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation

Cited By (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007100009A1 (en) 2006-03-03 2007-09-07 Ajinomoto Co., Inc. Method for production of l-amino acid
EP2055771A2 (en) 2006-03-23 2009-05-06 Ajinomoto Co., Inc. A method for producing an L-amino acid using bacterium of the Enterobacteriaceae family with attenuated expression of a gene coding for small RNA
WO2007125954A1 (en) 2006-04-28 2007-11-08 Ajinomoto Co., Inc. Microorganism capable of producing l-amino acid, and process for production of l-amino acid
EP2460873A1 (en) 2006-12-12 2012-06-06 Ajinomoto Co., Inc. A method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family with attenuated expression of any of the cynT, cynS, cynX or cynR genes or a combination thereof
WO2008090770A1 (en) 2007-01-22 2008-07-31 Ajinomoto Co., Inc. Microorganism capable of producing l-amino acid, and method for production of l-amino acid
WO2008102572A1 (en) 2007-02-20 2008-08-28 Ajinomoto Co., Inc. Method for production of l-amino acid or nucleic acid
WO2008102861A1 (en) 2007-02-22 2008-08-28 Ajinomoto Co., Inc. Method of producing l-amino acid
WO2009088049A1 (en) 2008-01-10 2009-07-16 Ajinomoto Co., Inc. Method for production of desired substance by fermentation process
EP2749652A2 (en) 2008-01-10 2014-07-02 Ajinomoto Co., Inc. A method for producing a target substance by fermentation
WO2009093703A1 (en) 2008-01-23 2009-07-30 Ajinomoto Co., Inc. Method of producing l-amino acid
EP2093291A1 (en) 2008-02-19 2009-08-26 Ajinomoto Co., Inc. A method for constructing an operon containing translationally coupled genes
WO2010027045A1 (en) 2008-09-08 2010-03-11 味の素株式会社 Microorganism capable of producing l-amino acid, and method for producing l-amino acid
WO2010084995A2 (en) 2009-01-23 2010-07-29 Ajinomoto Co.,Inc. A method for producing an l-amino acid
WO2011013707A1 (en) 2009-07-29 2011-02-03 味の素株式会社 Method for producing l-amino acid
WO2011043485A1 (en) 2009-10-05 2011-04-14 Ajinomoto Co.,Inc. A METHOD FOR PRODUCING AN L-CYSTEINE, L-CYSTINE, A DERIVATIVE OR PRECURSOR THEREOF OR A MIXTURE THEREOF USING A BACTERIUM OF Enterobacteriaceae FAMILY
EP2345667A2 (en) 2010-01-15 2011-07-20 Ajinomoto Co., Inc. A method for producing an L-amino acid using a bacterium of the enterobacteriaceae family
WO2011096554A1 (en) 2010-02-08 2011-08-11 味の素株式会社 MANUFACTURING METHOD FOR MUTANT rpsA GENE AND L-AMINO ACID
WO2011102305A2 (en) 2010-02-18 2011-08-25 Ajinomoto Co.,Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE Enterobacteriaceae FAMILY HAVING A MUTANT ADENYLATE CYCLASE
WO2012011595A1 (en) 2010-07-21 2012-01-26 Ajinomoto Co.,Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY HAVING ATTENUATED EXPRESSION OF THE astCADBE OPERON
WO2012011596A1 (en) 2010-07-21 2012-01-26 Ajinomoto Co.,Inc. A method for producing an l- amino acid using a bacterium of the enterobacteriaceae family with enhanced expression of the bssr gene
WO2012077739A1 (en) 2010-12-10 2012-06-14 味の素株式会社 Method for producing l-amino acid
EP2559754A2 (en) 2011-08-18 2013-02-20 Ajinomoto Co., Inc. Method for producing an L-amino acid using a bacterium of the family enterobacteriaceae having enhanced expression of the flagella formation and motility cascade genes
WO2013024904A1 (en) 2011-08-18 2013-02-21 Ajinomoto Co.,Inc. A method for producing an l-amino acid using a bacterium of the family enterobacteriaceae having enhanced expression of the flagella formation and motility cascade genes
WO2015005406A1 (en) 2013-07-09 2015-01-15 味の素株式会社 Method for manufacturing useful substance
EP3521433A1 (en) 2013-07-09 2019-08-07 Ajinomoto Co., Inc. Process for producing l-glutamic acid
WO2015050234A1 (en) 2013-10-02 2015-04-09 味の素株式会社 Ammonia control apparatus and ammonia control method
WO2015060314A1 (en) 2013-10-21 2015-04-30 味の素株式会社 Method for producing l-amino acid
EP3385389A1 (en) 2017-04-03 2018-10-10 Ajinomoto Co., Inc. Method for producing l-amino acid from fructose
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation

Also Published As

Publication number Publication date
CN1823162A (en) 2006-08-23
CN100471949C (en) 2009-03-25

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Effective date: 20051004