[go: up one dir, main page]

RU2007106185A - BACTERIA OF THE FAMILY ENTEPOBACTERIACEAE, WHICH EXPRESSION OF THE OPERON YcbPONME (SSUEADCB) WAS WEARED, IS A PRODUCER OF L-AMINO ACID AND A METHOD FOR PRODUCING L-AMINO ACID - Google Patents

BACTERIA OF THE FAMILY ENTEPOBACTERIACEAE, WHICH EXPRESSION OF THE OPERON YcbPONME (SSUEADCB) WAS WEARED, IS A PRODUCER OF L-AMINO ACID AND A METHOD FOR PRODUCING L-AMINO ACID Download PDF

Info

Publication number
RU2007106185A
RU2007106185A RU2007106185/13A RU2007106185A RU2007106185A RU 2007106185 A RU2007106185 A RU 2007106185A RU 2007106185/13 A RU2007106185/13 A RU 2007106185/13A RU 2007106185 A RU2007106185 A RU 2007106185A RU 2007106185 A RU2007106185 A RU 2007106185A
Authority
RU
Russia
Prior art keywords
amino acid
aromatic
group
ycbponme
operon
Prior art date
Application number
RU2007106185/13A
Other languages
Russian (ru)
Other versions
RU2392326C2 (en
Inventor
Дмитрий Владимирович Филипов (RU)
Дмитрий Владимирович Филипов
Эльвира Борисовна Ворошилова (RU)
Эльвира Борисовна Ворошилова
тинер Михаил Маркович Гус (RU)
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU)
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU), Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) filed Critical Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU)
Priority to RU2007106185/13A priority Critical patent/RU2392326C2/en
Priority to PCT/JP2008/052789 priority patent/WO2008105276A1/en
Publication of RU2007106185A publication Critical patent/RU2007106185A/en
Application granted granted Critical
Publication of RU2392326C2 publication Critical patent/RU2392326C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids

Landscapes

  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (11)

1. Бактерия-продуцент L-аминокислоты, принадлежащая к семейству Enterobacteriaceae, модифицированная таким образом, что экспрессия оперона ycbPONME в указанной бактерии ослаблена.1. The bacterium producing L-amino acids belonging to the Enterobacteriaceae family, modified so that the expression of the ycbPONME operon in the specified bacteria is weakened. 2. Бактерия по п.1, отличающаяся тем, что экспрессия указанного оперона ycbPONME ослаблена за счет инактивации указанного оперона ycbPONME.2. The bacterium according to claim 1, characterized in that the expression of the specified ycbPONME operon is weakened due to the inactivation of the specified ycbPONME operon. 3. Бактерия по п.1, отличающаяся тем, что указанная бактерия принадлежит к роду Escherichia.3. The bacterium according to claim 1, characterized in that said bacterium belongs to the genus Escherichia. 4. Бактерия по п.1, отличающаяся тем, что указанная бактерия принадлежит к роду Pantoea.4. The bacterium according to claim 1, characterized in that said bacterium belongs to the genus Pantoea. 5. Бактерия-продуцент L-аминокислоты по п.1, отличающаяся тем, что указанная L-аминокислота выбрана из группы, состоящей из ароматической L-аминокислоты и неароматической L-аминокислоты.5. The L-amino acid producing bacterium according to claim 1, characterized in that said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. 6. Бактерия-продуцент L-аминокислоты по п.5, отличающаяся тем, что указанная ароматическая L-аминокислота выбрана из группы, состоящей из L-фенилаланина, L-тирозина и L-триптофана.6. The L-amino acid producing bacterium according to claim 5, characterized in that said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tyrosine and L-tryptophan. 7. Бактерия-продуцент L-аминокислоты по п.5, отличающаяся тем, что указанная неароматическая L-аминокислота выбрана из группы, состоящей из L-треонина, L-лизина, L-цистеина, L-метионина, L-лейцина, L-изолейцина, L-валина, L-гистидина, L-глицина, L-серина, L-аланина, L-аспарагина, L-аспартата, L-глутамина, L-глутаминовой кислоты, L-пролина и L-аргинина.7. The L-amino acid producing bacterium according to claim 5, characterized in that said non-aromatic L-amino acid is selected from the group consisting of L-threonine, L-lysine, L-cysteine, L-methionine, L-leucine, L- isoleucine, L-valine, L-histidine, L-glycine, L-serine, L-alanine, L-asparagine, L-aspartate, L-glutamine, L-glutamic acid, L-proline and L-arginine. 8. Способ получения L-аминокислоты, включающий8. The method of obtaining L-amino acids, including выращивание бактерии по любому из пп.1-7 в питательной среде, вызывающее продукцию и накопление указанной L-аминокислоты в культуральной жидкости; иgrowing bacteria according to any one of claims 1 to 7 in a nutrient medium, causing production and accumulation of the specified L-amino acids in the culture fluid; and выделение указанной L-аминокислоты из культуральной жидкости.the allocation of the specified L-amino acids from the culture fluid. 9. Способ по п.8, отличающийся тем, что указанная L-аминокислота выбрана из группы, состоящей из ароматической L-аминокислоты и неароматической L-аминокислоты.9. The method of claim 8, wherein said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. 10. Способ по п.9, отличающийся тем, что указанная ароматическая L-аминокислота выбрана из группы, состоящей из L-фенилаланина, L-тирозина и L-триптофана.10. The method according to claim 9, characterized in that said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tyrosine and L-tryptophan. 11. Способ по п.9, отличающийся тем, что указанная неароматическая L-аминокислота выбрана из группы, состоящей из L-треонина, L-лизина, L-цистеина, L-метионина, L-лейцина, L-изолейцина, L-валина, L-гистидина, L-глицина, L-серина, L-аланина, L-аспарагина, L-аспартата, L-глутамина, L-глутаминовой кислоты, L-пролина и L-аргинина.11. The method according to claim 9, characterized in that said non-aromatic L-amino acid is selected from the group consisting of L-threonine, L-lysine, L-cysteine, L-methionine, L-leucine, L-isoleucine, L-valine , L-histidine, L-glycine, L-serine, L-alanine, L-asparagine, L-aspartate, L-glutamine, L-glutamic acid, L-proline and L-arginine.
RU2007106185/13A 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB) RU2392326C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)
PCT/JP2008/052789 WO2008105276A1 (en) 2007-02-20 2008-02-13 A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE ycbPONME OPERON (ssuEADCB OPERON)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)

Publications (2)

Publication Number Publication Date
RU2007106185A true RU2007106185A (en) 2008-08-27
RU2392326C2 RU2392326C2 (en) 2010-06-20

Family

ID=39402934

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)

Country Status (2)

Country Link
RU (1) RU2392326C2 (en)
WO (1) WO2008105276A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113136395A (en) * 2020-01-16 2021-07-20 南京理工大学 Expression vector of membrane protein YcbM and expression and purification method thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1694643A1 (en) * 1987-11-26 1991-11-30 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Strain of bacteria escherichia coli - a producer of l-threonine
JPH07155184A (en) * 1993-12-08 1995-06-20 Ajinomoto Co Inc Production of l-lysine by fermentation method
RU2229513C2 (en) * 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acids (variants)

Also Published As

Publication number Publication date
RU2392326C2 (en) 2010-06-20
WO2008105276A1 (en) 2008-09-04

Similar Documents

Publication Publication Date Title
RU2004137198A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE Enterobacteriaceae Family In Which The yafA Gene Is Inactivated
RU2006143864A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE EXPRESSION OF GENES cynT, cynS, cynX, OR cynR, OR THEIR COMBINATION IS DECREASED
RU2005101110A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE Glycogen biosynthesis pathway has been destroyed
RU2006123752A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE FAMILY Enterobacteriaceae, IN WHICH EXPRESSION OF THE OPERON rspAB IS DECREASED
RU2007104648A (en) METHOD FOR PRODUCING L-AMINO ACID USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE EXPRESSION OF THE tolC GENE IS DECREASED
RU2007106185A (en) BACTERIA OF THE FAMILY ENTEPOBACTERIACEAE, WHICH EXPRESSION OF THE OPERON YcbPONME (SSUEADCB) WAS WEARED, IS A PRODUCER OF L-AMINO ACID AND A METHOD FOR PRODUCING L-AMINO ACID
RU2007104650A (en) BACTERIA OF THE ENTERODACTERIACEAE FAMILY IN WHICH EXPRESSION OF THE OPERON YhcA-E, PRODUCER OF L-AMINO ACID AND METHOD FOR PRODUCING L-AMINO ACID, WAS WEARED
RU2007128314A (en) METHOD FOR PRODUCING L-AMINO ACID USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH EXPRESSION OF THE yncD GENE IS DECREASED
RU2006100896A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE yfeH GENE IS INACTIVATED
RU2006100895A (en) METHOD FOR PRODUCING L-AMINO ACID USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE lrhA GENE IS INACTIVATED
RU2006118967A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE ENTEROBACTERIACEAE FAMILY IN WHICH EXPRESSION OF THE RCSA GENE IS WEAKEN
RU2007132476A (en) The Enterobacteriaceae family bacterium, in which the expression of the hfq gene is weakened, is a producer of L-amino acids and a method for producing L-FVBYJRBCKJNS
RU2006112261A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE Enterobacteriaceae Family In Which Expression Of The ybdA Gene Is Weakened
RU2007105212A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH EXPRESSION OF THE ecpD GENE OR OPERON ecpD-htrE is weakened
RU2006116167A (en) METHOD FOR PRODUCING L-AMINO ACID USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE EXPRESSION OF THE KEFB GENE IS DECREASED
RU2006123753A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE Enterobacteriaceae Family In Which Expression Of The yraH-R Gene Cluster Is Weakened
RU2006112259A (en) METHOD FOR PRODUCING L-AMINO ACID USING THE ENTEROBACTERIACEAE FAMILY BACTERIA MODIFIED IN SUCH A PERFORMANCE THAT THE KERLI TYPE FORMATION IS DISCONNECTED IN IT
RU2005123423A (en) Bacteria of the Enterobacteriaceae family in which the pnp gene is inactivated, the producer of L-amino acids and the method for producing L-amino acids
RU2007112273A (en) Bacteria of the Enterobacteriaceae family in which msbA gene expression is weakened is a producer of L-amino acid and a method for producing L-amino acid
RU2006101788A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE fdrA GENE IS INACTIVATED
RU2007112271A (en) METHOD FOR PRODUCING L-Amino Acids USING THE Enterobacteriaceae Bacteria in Which Expression of the ygiD Gene Is Weakened
RU2007131006A (en) METHOD FOR PRODUCING L-AMINO ACID USING THE Enterobacteriaceae FAMILY, IN WHICH EXPRESSION OF THE chaC GENE OR OPERA chaBC is Weakened
RU2006104648A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE EXPRESSION OF THE PAS GENE IS DECREASED
RU2005106347A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE NAC GENE IS INACTIVATED
RU2005138194A (en) METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE YAFA GENE IS INACTIVATED