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PE20040537A1 - Fitasas modificadas - Google Patents

Fitasas modificadas

Info

Publication number
PE20040537A1
PE20040537A1 PE2003000534A PE2003000534A PE20040537A1 PE 20040537 A1 PE20040537 A1 PE 20040537A1 PE 2003000534 A PE2003000534 A PE 2003000534A PE 2003000534 A PE2003000534 A PE 2003000534A PE 20040537 A1 PE20040537 A1 PE 20040537A1
Authority
PE
Peru
Prior art keywords
model
fitasa
polypeptide
seq
amino acid
Prior art date
Application number
PE2003000534A
Other languages
English (en)
Inventor
Jan Metske Van Der Laan
Stefanus Cornelis Hendrikus Josef Turk
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Publication of PE20040537A1 publication Critical patent/PE20040537A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE REFIERE A UN POLIPEPTIDO TAL QUE CUANDO ES ALINEADO CON LA FITASA MODELO ASPERGILLUS NIGER, CONTIENE UNA MODIFICACION SELECCIONADA DEL GRUPO FORMADO DE: A) SUSTITUICION DE UN AMINOACIDO PRESENTE EN LA FITASA MODELO; B) SUPRESION DE UN AMINOACIDO PRESENTE EN LA FITASA MODELO; C) INSERCION DE UN AMINOACIDO. LA ALINEACION DEL POLIPEPTIDO PRESENTA AL MENOS DE UN 91-99% DE HOMOLOGIA Y AL MENOS ESTA MODIFICADO EN UNA DE LAS SIGUIENTES POSICIONES: 3, 78, 163, 180, 182,194, 211, 215, 242, 254, 269, 414, 428, 440. DICHO POLIPEPTIDO ES DE LA SEQ ID N°: 3, SEQ ID N°: 5 O SEQ ID N°: 7 Y POSEE LAS MISMAS PROPIEDADES FABORABLES QUE LA FITASA MODELO COMBINADAS CON UNA GRAN ESTABILIDAD Y ACTIVIDAD A TEMPERATURAS Y HUMEDAD ALTAS
PE2003000534A 2002-05-30 2003-05-30 Fitasas modificadas PE20040537A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02100608 2002-05-30

Publications (1)

Publication Number Publication Date
PE20040537A1 true PE20040537A1 (es) 2004-11-04

Family

ID=29595046

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2003000534A PE20040537A1 (es) 2002-05-30 2003-05-30 Fitasas modificadas

Country Status (16)

Country Link
EP (1) EP1513931A2 (es)
JP (1) JP2005533492A (es)
KR (1) KR20050038593A (es)
CN (1) CN1656217A (es)
AR (2) AR039938A1 (es)
AU (1) AU2003238448A1 (es)
BR (1) BR0311287A (es)
CA (1) CA2485513A1 (es)
MX (1) MXPA04011224A (es)
NO (1) NO20044802L (es)
PE (1) PE20040537A1 (es)
PL (1) PL372824A1 (es)
RU (1) RU2329301C2 (es)
TW (2) TW200401033A (es)
WO (1) WO2003102174A2 (es)
ZA (1) ZA200410369B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
WO2007112739A1 (en) 2006-04-04 2007-10-11 Novozymes A/S Phytase variants
DE102006053059A1 (de) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
CN102854158B (zh) * 2011-07-01 2016-01-20 北京昕大洋科技发展有限公司 一种快速测定植酸酶耐温性能的方法
CN105441406A (zh) * 2014-08-05 2016-03-30 北京大学 植酸酶变体
CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
CN117683745A (zh) * 2020-04-29 2024-03-12 南京百斯杰生物工程有限公司 一种亲本植酸酶变体

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2316082A (en) * 1996-08-13 1998-02-18 Finnfeeds Int Ltd Phytase
EP1151088B1 (en) * 1999-01-22 2006-08-30 Novozymes A/S Improved phytases

Also Published As

Publication number Publication date
EP1513931A2 (en) 2005-03-16
BR0311287A (pt) 2005-03-29
WO2003102174A2 (en) 2003-12-11
MXPA04011224A (es) 2005-01-25
KR20050038593A (ko) 2005-04-27
JP2005533492A (ja) 2005-11-10
RU2329301C2 (ru) 2008-07-20
ZA200410369B (en) 2006-06-28
CN1656217A (zh) 2005-08-17
PL372824A1 (en) 2005-08-08
AR039939A1 (es) 2005-03-09
TW200401033A (en) 2004-01-16
CA2485513A1 (en) 2003-12-11
NO20044802L (no) 2004-12-08
RU2004139054A (ru) 2005-06-27
AU2003238448A1 (en) 2003-12-19
TW200403341A (en) 2004-03-01
WO2003102174A3 (en) 2004-03-25
AR039938A1 (es) 2005-03-09

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