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AR039938A1 - Fitasas modificadas - Google Patents

Fitasas modificadas

Info

Publication number
AR039938A1
AR039938A1 ARP030101936A ARP030101936A AR039938A1 AR 039938 A1 AR039938 A1 AR 039938A1 AR P030101936 A ARP030101936 A AR P030101936A AR P030101936 A ARP030101936 A AR P030101936A AR 039938 A1 AR039938 A1 AR 039938A1
Authority
AR
Argentina
Prior art keywords
phytase
modified
phytases
model
compared
Prior art date
Application number
ARP030101936A
Other languages
English (en)
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of AR039938A1 publication Critical patent/AR039938A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente describe fitasas modificadas. Estas fitasas están modificadas en comparación con una fitasa modelo en diversas posiciones, en particular para incrementar la termoestabilidad de la fitasa modificada en comparación con la de la fitasa modelo. Las fitasas modificadas han retenido otras propiedades favorables de la fitasa de Aspergillus niger, en particular porque en la fitasa modificada se han retenido residuos de aminoácidos específicos de la fitasa de Aspergillus niger. Polinucleótidos que codifican para dichas fitasas, vector, célula huésped, procedimientos para la producción de los polipéptidos y composición que los comprende. Reivindicación 1: Un polipéptido, caracterizado porque cuando se alinea con una fitasa modelo está ,modificado en comparación con dicha fitasa modelo en al menos una de las siguientes posiciones: 5, 6, 13, 19, 21, 29, 31, 36, 39, 43, 53, 69, 78, 81, 85, 87, 99, 112, 113, 122, 125, 126, 128,137,147, 148, 157,160, 163, 165, 169, 172, 176, 178, 180, 181, 182, 183, 189, 194, 197, 201, 203, 211, 213, 215, 218, 222, 223, 225, 232, 233, 242, 246, 247, 248, 249, 250, 251, 252, 254, 269, 291, 296, 310, 312, 315, 322, 330, 342, 346, 362, 365, 367, 368, 372, 374, 375, 382, 384, 395, 414, 417, 425, 428, 438, 440.
ARP030101936A 2002-05-30 2003-05-30 Fitasas modificadas AR039938A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02100608 2002-05-30

Publications (1)

Publication Number Publication Date
AR039938A1 true AR039938A1 (es) 2005-03-09

Family

ID=29595046

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP030101936A AR039938A1 (es) 2002-05-30 2003-05-30 Fitasas modificadas
ARP030101937A AR039939A1 (es) 2002-05-30 2003-05-30 Fitasas modificadas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP030101937A AR039939A1 (es) 2002-05-30 2003-05-30 Fitasas modificadas

Country Status (16)

Country Link
EP (1) EP1513931A2 (es)
JP (1) JP2005533492A (es)
KR (1) KR20050038593A (es)
CN (1) CN1656217A (es)
AR (2) AR039938A1 (es)
AU (1) AU2003238448A1 (es)
BR (1) BR0311287A (es)
CA (1) CA2485513A1 (es)
MX (1) MXPA04011224A (es)
NO (1) NO20044802L (es)
PE (1) PE20040537A1 (es)
PL (1) PL372824A1 (es)
RU (1) RU2329301C2 (es)
TW (2) TW200401033A (es)
WO (1) WO2003102174A2 (es)
ZA (1) ZA200410369B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
WO2007112739A1 (en) 2006-04-04 2007-10-11 Novozymes A/S Phytase variants
DE102006053059A1 (de) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
CN102854158B (zh) * 2011-07-01 2016-01-20 北京昕大洋科技发展有限公司 一种快速测定植酸酶耐温性能的方法
CN105441406A (zh) * 2014-08-05 2016-03-30 北京大学 植酸酶变体
CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
CN117683745A (zh) * 2020-04-29 2024-03-12 南京百斯杰生物工程有限公司 一种亲本植酸酶变体

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2316082A (en) * 1996-08-13 1998-02-18 Finnfeeds Int Ltd Phytase
EP1151088B1 (en) * 1999-01-22 2006-08-30 Novozymes A/S Improved phytases

Also Published As

Publication number Publication date
EP1513931A2 (en) 2005-03-16
BR0311287A (pt) 2005-03-29
WO2003102174A2 (en) 2003-12-11
MXPA04011224A (es) 2005-01-25
KR20050038593A (ko) 2005-04-27
JP2005533492A (ja) 2005-11-10
RU2329301C2 (ru) 2008-07-20
ZA200410369B (en) 2006-06-28
CN1656217A (zh) 2005-08-17
PL372824A1 (en) 2005-08-08
AR039939A1 (es) 2005-03-09
TW200401033A (en) 2004-01-16
CA2485513A1 (en) 2003-12-11
NO20044802L (no) 2004-12-08
RU2004139054A (ru) 2005-06-27
AU2003238448A1 (en) 2003-12-19
TW200403341A (en) 2004-03-01
WO2003102174A3 (en) 2004-03-25
PE20040537A1 (es) 2004-11-04

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