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PE20010697A1 - Gen de streptomyces avermitilis que dirige la relacion de avermectinas b2:b1 - Google Patents

Gen de streptomyces avermitilis que dirige la relacion de avermectinas b2:b1

Info

Publication number
PE20010697A1
PE20010697A1 PE2000000813A PE0008132000A PE20010697A1 PE 20010697 A1 PE20010697 A1 PE 20010697A1 PE 2000000813 A PE2000000813 A PE 2000000813A PE 0008132000 A PE0008132000 A PE 0008132000A PE 20010697 A1 PE20010697 A1 PE 20010697A1
Authority
PE
Peru
Prior art keywords
avermitilis
avec
sequence
class
avermectins
Prior art date
Application number
PE2000000813A
Other languages
English (en)
Inventor
Kim Jonelle Stutzman-Engwall
Original Assignee
Pfizer Prod Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Prod Inc filed Critical Pfizer Prod Inc
Publication of PE20010697A1 publication Critical patent/PE20010697A1/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/60Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/60Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C12P19/623Avermectin; Milbemycin; Ivermectin; C-076

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

SE REFIERE A POLINUCLEOTIDOS QUE COMPRENDE UNA SECUENCIA DE NUCLEOTIDOS QUE POR LO MENOS ES IGUAL A UNA VARIANTE DEGENERADA DEL ALELO DE AVEC DE STREPTOMYCES AVERMITILIS, LA SECUENCIA CODIFICANTE DEL PRODUCTO GENICO AVEC DEL PLASMIDO pSE186 O LA SECUENCIA DE NUCLEOTIDOS DE LA ORF DE AVEC DE S.AVERMITILIS QUE COMPRENDE ADEMAS LA SECUENCIA DE NUCLEOTIDOS UNA O MAS MUTACIONES TALES QUE LAS CELULAS DE S. AVERMITILIS EN LAS QUE EL ALELO DE AVEC DE TIPO SILVESTRE SE HA INACTIVADO Y EXPRESAN POLINUCLEOTIDO QUE COMPRENDE LA SECUENCIA DE NUCLEOTIDO MUTADA, PRODUCEN UNA RELACION DE AVERMECTINAS DE CLASES 2:1 QUE ES REDUCIDA EN COMPARACION CON LA RELACION DE AVERMECTINAS DE CLASES 2:1 PRODUCIDAS POR CELULAS DE S.AVERMITILIS QUE EN SU LUGAR EXPRESAN SOLO EL ALELO DE AVEC DE TIPO SILVESTRE, SIENDO LAS AVERMECTINAS DE CLASES 2:1 SON CICLOHEXIL B2, CICLOHEXIL B1 AVERMECTINAS. TAMBIEN SE REFIERE A VECTORES, CELULAS HUESPED, CEPAS MUTANTES DE S. AVERMITILIS EN LAS QUE EL GEN AVEC SE HA INACTIVADO O MUTADO PARA CAMBIAR LA RELACION O CANTIDAD DE AVERMECTINAS DE CLASES 2:1 PRODUCIDAS
PE2000000813A 1999-08-12 2000-08-10 Gen de streptomyces avermitilis que dirige la relacion de avermectinas b2:b1 PE20010697A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/372,934 US6248579B1 (en) 1998-02-13 1999-08-12 Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins

Publications (1)

Publication Number Publication Date
PE20010697A1 true PE20010697A1 (es) 2001-07-05

Family

ID=23470234

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2000000813A PE20010697A1 (es) 1999-08-12 2000-08-10 Gen de streptomyces avermitilis que dirige la relacion de avermectinas b2:b1

Country Status (37)

Country Link
US (8) US6248579B1 (es)
EP (1) EP1200605B1 (es)
JP (2) JP3688640B2 (es)
KR (1) KR100486006B1 (es)
CN (1) CN1186447C (es)
AP (2) AP2002002419A0 (es)
AR (1) AR021450A1 (es)
AT (1) ATE283363T1 (es)
AU (1) AU779756B2 (es)
BG (1) BG106478A (es)
BR (1) BR0013093A (es)
CA (1) CA2381427A1 (es)
CO (1) CO5290296A1 (es)
CZ (1) CZ2002297A3 (es)
DE (1) DE60016231T2 (es)
EA (1) EA004569B1 (es)
ES (1) ES2231214T3 (es)
GT (2) GT200000138A (es)
HK (1) HK1046536B (es)
HR (1) HRP20020131A2 (es)
HU (1) HUP0202517A2 (es)
IL (1) IL147562A0 (es)
IS (1) IS6249A (es)
MA (1) MA26811A1 (es)
MX (1) MXPA02001514A (es)
NO (1) NO20020665D0 (es)
OA (1) OA11997A (es)
PA (1) PA8499201A1 (es)
PE (1) PE20010697A1 (es)
PL (1) PL353770A1 (es)
PT (1) PT1200605E (es)
SK (1) SK1382002A3 (es)
TN (1) TNSN00170A1 (es)
UY (1) UY26287A1 (es)
WO (1) WO2001012822A1 (es)
YU (1) YU8402A (es)
ZA (1) ZA200201071B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU230600B1 (hu) * 1999-08-12 2017-02-28 Zoetis Services Llc Az avermektinek B2:B1 arányát befolyásoló Streptomyces avermitilis gén
AU2003210850A1 (en) 2002-02-05 2003-09-02 Bristol-Myers Squibb Company N-substituted3-hydroxy-4-pyridinones and pharmaceuticals containing thereof
DE60326824D1 (de) * 2002-02-12 2009-05-07 Pfizer Prod Inc Streptomyces avermitilis gen zur regulierung des verhältnisses der b2:b1 avermectine
US7943160B2 (en) * 2002-05-09 2011-05-17 Scimetrics Limited Corp. Pest control methods
EP1597271B1 (de) * 2003-01-31 2013-03-06 Max-Delbrück-Centrum Für Molekulare Medizin Peptide gegen kälteunverträglichkeit hervorrufende autoantikörper und ihre verwendung
KR100759130B1 (ko) 2005-02-12 2007-09-19 휴메드 주식회사 항 인테그린 항체 코팅스텐트 및 그의 제조방법
CN106148216B (zh) 2015-03-27 2019-06-04 浙江海正药业股份有限公司 一种链霉菌及其生产米尔贝霉素a3的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5238848A (en) 1987-01-23 1993-08-24 Pfizer Inc Cultures for production of avermectins
US5234831A (en) 1987-01-23 1993-08-10 Pfizer Inc Cultures for production of B avermectins
US5525506A (en) 1987-01-23 1996-06-11 Pfizer Inc. Process for production of avermectins and cultures therefor
ES2054822T3 (es) 1987-10-23 1994-08-16 Pfizer Procedimiento para la produccion de agliconas de avermectinas y sus cultivos.
US5240850A (en) 1987-10-23 1993-08-31 Pfizer Inc. Cultures for production of avermectin aglycones
US5252474A (en) 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
ES2079436T3 (es) 1989-03-31 1996-01-16 Merck & Co Inc Clonacion de genes de streptomyces avermitilis para biosintesis de avermectinas y procedimientos para su uso.
DK0711349T3 (da) 1993-07-30 2003-11-24 Pfizer Gener, der koder for forgrenet alfaketosyredehydrogenasekompleks fra Streptomyces avermitilis
DK0722453T3 (da) * 1993-10-05 1999-12-27 Pfizer Fremgangsmåde og antiparasitiske mellemprodukter til doramectin
FI942725A7 (fi) 1993-12-16 1995-06-17 Pfizer Haaraketjuista alfaketohappodenydrogenaasikompleksia koodaavia geenejä Streptomyces avermitiliksesta
HRP20000539A2 (en) * 1998-02-13 2001-04-30 Pfizer Prod Inc Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

Also Published As

Publication number Publication date
IL147562A0 (en) 2002-08-14
AP2002002419A0 (en) 2002-03-31
US20030129636A1 (en) 2003-07-10
OA11997A (en) 2006-04-18
PT1200605E (pt) 2005-04-29
NO20020665L (no) 2002-02-11
AU5702100A (en) 2001-03-13
IS6249A (is) 2002-01-25
ZA200201071B (en) 2003-06-25
CO5290296A1 (es) 2003-06-27
ATE283363T1 (de) 2004-12-15
GT200000138CA (es) 2002-02-02
US20030134313A1 (en) 2003-07-17
HRP20020131A2 (en) 2003-12-31
HK1046536B (zh) 2005-05-13
CA2381427A1 (en) 2001-02-22
CZ2002297A3 (cs) 2002-08-14
TNSN00170A1 (fr) 2005-11-10
PA8499201A1 (es) 2001-12-14
US20010016350A1 (en) 2001-08-23
NO20020665D0 (no) 2002-02-11
US7029887B2 (en) 2006-04-18
DE60016231D1 (de) 2004-12-30
US6511841B2 (en) 2003-01-28
CN1370239A (zh) 2002-09-18
EP1200605A1 (en) 2002-05-02
GT200000138A (es) 2002-02-02
MXPA02001514A (es) 2002-07-02
AU779756B2 (en) 2005-02-10
SK1382002A3 (en) 2002-11-06
PL353770A1 (en) 2003-12-01
US6833263B2 (en) 2004-12-21
JP2003507018A (ja) 2003-02-25
YU8402A (sh) 2006-03-03
ES2231214T3 (es) 2005-05-16
EA004569B1 (ru) 2004-06-24
US20050196777A1 (en) 2005-09-08
JP3688640B2 (ja) 2005-08-31
EA200200057A1 (ru) 2002-06-27
US20030124607A1 (en) 2003-07-03
WO2001012822A1 (en) 2001-02-22
JP2005198658A (ja) 2005-07-28
BG106478A (bg) 2004-02-27
AR021450A1 (es) 2002-07-17
KR100486006B1 (ko) 2005-05-03
HK1046536A1 (en) 2003-01-17
HUP0202517A2 (hu) 2002-11-28
US7259241B2 (en) 2007-08-21
EP1200605B1 (en) 2004-11-24
US6248579B1 (en) 2001-06-19
US6509159B2 (en) 2003-01-21
US20030129635A1 (en) 2003-07-10
DE60016231T2 (de) 2005-12-08
MA26811A1 (fr) 2004-12-20
AP2002002417A0 (en) 2002-03-31
US20010024818A1 (en) 2001-09-27
UY26287A1 (es) 2001-03-16
KR20020022809A (ko) 2002-03-27
CN1186447C (zh) 2005-01-26
BR0013093A (pt) 2002-08-13

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