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IN2012DN00801A - - Google Patents

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Publication number
IN2012DN00801A
IN2012DN00801A IN801DEN2012A IN2012DN00801A IN 2012DN00801 A IN2012DN00801 A IN 2012DN00801A IN 801DEN2012 A IN801DEN2012 A IN 801DEN2012A IN 2012DN00801 A IN2012DN00801 A IN 2012DN00801A
Authority
IN
India
Prior art keywords
adamts
expression
methods
useful
proteins
Prior art date
Application number
Inventor
Grillberger Leopold
Spenger Alexandra
Hasslacher Meinhard
Grillberger Rana
Reiter Manfred
Original Assignee
Baxter Int
Baxter Healthcare Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Int, Baxter Healthcare Sa filed Critical Baxter Int
Publication of IN2012DN00801A publication Critical patent/IN2012DN00801A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)

Abstract

The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS 13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.
IN801DEN2012 2009-07-31 2010-07-30 IN2012DN00801A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (1)

Publication Number Publication Date
IN2012DN00801A true IN2012DN00801A (en) 2015-06-26

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
IN801DEN2012 IN2012DN00801A (en) 2009-07-31 2010-07-30

Country Status (21)

Country Link
US (7) US8313926B2 (en)
EP (1) EP2459702B1 (en)
JP (5) JP5451884B2 (en)
KR (5) KR101574056B1 (en)
CN (2) CN102549145B (en)
AU (1) AU2010278751B2 (en)
BR (2) BR112012002151B1 (en)
CA (1) CA2769361A1 (en)
CO (1) CO6501191A2 (en)
EA (1) EA023193B1 (en)
ES (1) ES2583258T3 (en)
HR (1) HRP20160783T1 (en)
HU (1) HUE029110T2 (en)
IN (1) IN2012DN00801A (en)
MX (2) MX2012001261A (en)
NZ (1) NZ597727A (en)
PL (1) PL2459702T3 (en)
PT (1) PT2459702T (en)
SG (1) SG178194A1 (en)
SI (1) SI2459702T1 (en)
WO (1) WO2011014838A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2459702B1 (en) * 2009-07-31 2016-05-04 Baxalta GmbH Cell culture medium for adamts protein expression
HRP20250948T1 (en) 2010-07-08 2025-10-10 Takeda Pharmaceutical Company Limited PROCEDURE FOR PRODUCTION OF RECOMBINANT HIGH MOLECULAR VWF IN CELL CULTURE
KR102358951B1 (en) 2011-07-01 2022-02-08 암젠 인크 Mammalian cell culture
WO2014062535A1 (en) 2012-10-15 2014-04-24 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
US9611455B2 (en) 2013-03-13 2017-04-04 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (en) * 2013-03-15 2015-09-30 Regeneron Pharma SERUM FREE CELL CULTIVATION MEDIA
WO2015055324A1 (en) * 2013-10-14 2015-04-23 Ares Trading S.A New medium for high performance mammalian fed-batch cultures
HUE042277T2 (en) 2014-06-04 2019-06-28 Amgen Inc Methods of collecting proteins from mammalian cell cultures
KR101867134B1 (en) * 2015-03-23 2018-06-12 한양대학교 산학협력단 Cell culture media for production of target material with high yield using mammalian cell, method for culturing cells and production of target material using the cell culture media
PL3298138T3 (en) * 2015-05-22 2022-12-05 Dupont Nutrition Biosciences Aps Acetolactate decarboxylase
TWI797060B (en) 2015-08-04 2023-04-01 美商再生元醫藥公司 Taurine supplemented cell culture medium and methods of use
AU2017307582C1 (en) 2016-08-04 2021-05-06 Takeda Pharmaceutical Company Limited Use of adamts13 for treating, ameliorating and/or preventing vaso-occlusive crisis in sickle cell disease, acute lung injury and/or acute respiratory distress syndrome
SG11201903890QA (en) * 2016-11-04 2019-05-30 Baxalta Inc Adeno-associated virus purification methods
AU2018298039B2 (en) * 2017-07-06 2023-02-02 Regeneron Pharmaceuticals, Inc. Cell culture process for making a glycoprotein
IL319295A (en) * 2018-03-16 2025-04-01 Genzyme Corp Methods for improving cell viability in a production bioreactor
CA3103443A1 (en) * 2018-06-18 2019-12-26 Synthetic Biologics, Inc. Methods of making alkaline phosphatase agents
EP4037704B1 (en) 2019-10-04 2025-12-03 The Children's Medical Center Corporation Adamts13 treatment to enhance graft survival
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
AU2020398125C1 (en) 2019-12-06 2023-05-11 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
CN115836126A (en) 2020-04-02 2023-03-21 武田药品工业株式会社 ADAMTS13 variants, compositions, and uses thereof
AU2021268026A1 (en) 2020-05-08 2023-01-19 Regeneron Pharmaceuticals, Inc. VEGF traps and mini-traps and methods for treating ocular disorders and cancer
WO2021234458A1 (en) 2020-05-22 2021-11-25 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
WO2021246557A1 (en) * 2020-06-05 2021-12-09 주식회사 프로앱텍 Urate oxidase-albumin conjugate having certain number of conjugated albumins, and method for producing same
CN111808822B (en) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 Feed supplement liquid for cell culture and method for improving recombinant HEK293 cell protein expression quantity

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
JPH0753104B2 (en) * 1989-02-28 1995-06-07 秀明 山田 Bacterial culture method
CZ280901B6 (en) 1988-10-06 1996-05-15 Hideaki Yamada Rhodococcus rhodochrous bacteria cultivation method
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
ATE542891T1 (en) 1994-11-10 2012-02-15 Baxter Healthcare Sa METHOD FOR PRODUCING BIOLOGICAL PRODUCTS IN PROTEIN-FREE CULTURE
DE69608668T2 (en) 1995-02-23 2001-02-01 Quest International B.V., Naarden PEPTIDES FOR TISSUE AND CELL CULTURE MEDIA
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP2221361A3 (en) 1996-08-30 2011-02-09 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
IT1285158B1 (en) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio SOLUBLE POLYPEPTIDES WITH THE SERINO-PROTEASIS ACTIVITY OF NS3 OF THE HEPATITIS C VIRUS, AND PROCEDURE FOR THEIR PREPARATION AND
CN1102921C (en) 1996-10-07 2003-03-12 索罗蒂亚公司 Process for hydroxylation of benzene
ATE399855T1 (en) 1996-10-10 2008-07-15 Invitrogen Corp ANIMAL CELL CULTURE MEDIUM WITH PLANT NUTRIENTS
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
AT407255B (en) 1997-06-20 2001-02-26 Immuno Ag RECOMBINANT CELL CLONE WITH INCREASED STABILITY IN SERUM- AND PROTEIN-FREE MEDIUM AND METHOD FOR OBTAINING THE STABLE CELL CLONE
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (en) 1999-06-02 2002-07-25 Baxter Ag MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE
US6926894B2 (en) 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
EP1576148B1 (en) 2002-12-23 2012-10-03 FUJIFILM Manufacturing Europe B.V. Process for coating cell-culture support
CA2562683A1 (en) * 2004-04-16 2005-12-08 Wyeth Adamts-8 proteins and uses thereof
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (en) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd Enzyme water production equipment
JP4880997B2 (en) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Method for stabilizing ADAMTS13
ES2790887T3 (en) * 2006-01-04 2020-10-29 Baxalta Inc Cell culture medium without oligopeptides
WO2008077217A2 (en) * 2006-12-22 2008-07-03 Springer Carrier Ltda. Fresh air command lever for an air conditioning unit
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
CA2709890A1 (en) * 2007-12-27 2009-07-09 Andre Giovagnoli Cell culture processes
EP2459702B1 (en) * 2009-07-31 2016-05-04 Baxalta GmbH Cell culture medium for adamts protein expression

Also Published As

Publication number Publication date
US20170051270A1 (en) 2017-02-23
MX344970B (en) 2017-01-12
AU2010278751A1 (en) 2012-02-09
CO6501191A2 (en) 2012-08-15
KR20120046301A (en) 2012-05-09
JP5451884B2 (en) 2014-03-26
BR122021005965B1 (en) 2022-01-25
EA023193B1 (en) 2016-05-31
HK1171790A1 (en) 2013-04-05
ES2583258T3 (en) 2016-09-20
US9441216B2 (en) 2016-09-13
JP2018093881A (en) 2018-06-21
JP2021112210A (en) 2021-08-05
CN104962539B (en) 2019-09-17
US20130071906A1 (en) 2013-03-21
MX2012001261A (en) 2012-05-22
CA2769361A1 (en) 2011-02-03
EP2459702B1 (en) 2016-05-04
JP2013500724A (en) 2013-01-10
BR112012002151B1 (en) 2021-07-06
US20110086413A1 (en) 2011-04-14
NZ597727A (en) 2013-08-30
BR112012002151A2 (en) 2020-03-03
CN104962539A (en) 2015-10-07
HRP20160783T1 (en) 2016-08-12
AU2010278751B2 (en) 2014-05-15
KR20150044981A (en) 2015-04-27
EP2459702A1 (en) 2012-06-06
PT2459702T (en) 2016-08-02
JP6567707B2 (en) 2019-08-28
KR20170081747A (en) 2017-07-12
US20200308566A1 (en) 2020-10-01
JP2019201661A (en) 2019-11-28
JP2016144471A (en) 2016-08-12
SG178194A1 (en) 2012-03-29
US20150329848A1 (en) 2015-11-19
KR20170140411A (en) 2017-12-20
US20190071660A1 (en) 2019-03-07
US8313926B2 (en) 2012-11-20
JP2014061005A (en) 2014-04-10
KR20150138425A (en) 2015-12-09
JP7083059B2 (en) 2022-06-09
JP6883071B2 (en) 2021-06-09
US10072254B2 (en) 2018-09-11
US11254921B2 (en) 2022-02-22
KR101912552B1 (en) 2018-10-26
US20140329294A1 (en) 2014-11-06
HUE029110T2 (en) 2017-02-28
PL2459702T3 (en) 2016-12-30
JP5936232B2 (en) 2016-06-22
EA201270224A1 (en) 2012-09-28
US9127265B2 (en) 2015-09-08
US8759026B2 (en) 2014-06-24
KR101756354B1 (en) 2017-07-26
KR101808496B1 (en) 2017-12-12
KR101574056B1 (en) 2015-12-02
WO2011014838A1 (en) 2011-02-03
CN102549145A (en) 2012-07-04
SI2459702T1 (en) 2016-11-30
US10724024B2 (en) 2020-07-28
JP6511007B2 (en) 2019-05-08
KR101573972B1 (en) 2015-12-02
CN102549145B (en) 2015-05-20

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