FI97549B - Förfarande för framställning av främmande proteiner i Streptomycesceller - Google Patents
Förfarande för framställning av främmande proteiner i Streptomycesceller Download PDFInfo
- Publication number
- FI97549B FI97549B FI882059A FI882059A FI97549B FI 97549 B FI97549 B FI 97549B FI 882059 A FI882059 A FI 882059A FI 882059 A FI882059 A FI 882059A FI 97549 B FI97549 B FI 97549B
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- Prior art keywords
- plasmid
- gene
- sequence
- tendamistat
- fusion protein
- Prior art date
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- 108010069020 alanyl-prolyl-glycine Proteins 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003392 amylase inhibitor Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 239000012131 assay buffer Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 108010016616 cysteinylglycine Proteins 0.000 description 1
- 108010069495 cysteinyltyrosine Proteins 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 108010049041 glutamylalanine Proteins 0.000 description 1
- XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
- 108010072405 glycyl-aspartyl-glycine Proteins 0.000 description 1
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 108010012058 leucyltyrosine Proteins 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 108010048818 seryl-histidine Proteins 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 108010061238 threonyl-glycine Proteins 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
- 108010035534 tyrosyl-leucyl-alanine Proteins 0.000 description 1
- 108010078580 tyrosylleucine Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Claims (8)
1. Förfarande för framställning av fusionsprotein, kännetecknat därav, att det önskade proteinets 5 strukturgen kopplas tili 3'-ändan av den kodande strängen av en eventuellt modifierad tendamistatgen, vilken kodar i tabell I angivet tendamistat eller en modifikation därav, som fortfarande förmär reagera reversibelt med spesifika reseptorer, denna genkonstruktion uttrycks i Streptomyces-10 värdceller, och det utsöndrade fusionsproteinet isoleras ur odlingssupernatanten.
2. Förfarande enligt patentkrav 1, kännetecknat därav, att tendamistatgenen förkortats ut-gäende frän den kodande strängens 3'-ända.
3. Genkonstruktion, kännetecknad därav, att man till 3'-ändan av den däri ingäende kodande strängen av en eventuellt modifierad tendamistatgen, vilken kodar i tabell I angivet tendamistat eller en modifikation därav, som fortfarande förmär reagera reversibelt 20 med spesifika reseptorer, kopplat en strukturgen för ett annat protein.
4. Genkonstruktion enligt patentkrav 3, kännetecknad därav, att tendamistatgenen förkortats ut-gäende frän den kodande strängens 3'-ända.
5. Vektor, kännetecknad därav, att den innehäller en genkonstruktion enligt patentkrav 3 eller 4.
6. Streptomyces-cell, kännetecknad därav, att den innehäller en vektor enligt patentkrav 5.
7. Fusionsprotein, kännetecknat därav, 30 att det innehäller en N-terminal del, som utgörs av den i tabell I angivna tendamistatsekvensen eller en modifikation därav, som fortfarande förmär reagera reversibelt med specifikä receptorer.
8. Användning av ett fusionsprotein enligt patent-35 krav 7 eller som erhällits enligt patentkrav 1 eller 2, kännetecknad därav, att det sker för att fram-ställa ett eventuellt modifierat tendamistat och/eller dess fusionspartner.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3714866 | 1987-05-05 | ||
| DE19873714866 DE3714866A1 (de) | 1987-05-05 | 1987-05-05 | Verfahren zur herstellung von fremdproteinen in streptomyceten |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI882059A0 FI882059A0 (sv) | 1988-05-03 |
| FI882059L FI882059L (sv) | 1988-11-06 |
| FI97549B true FI97549B (sv) | 1996-09-30 |
| FI97549C FI97549C (sv) | 1997-01-10 |
Family
ID=6326841
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI882059A FI97549C (sv) | 1987-05-05 | 1988-05-03 | Förfarande för framställning av främmande proteiner i Streptomycesceller |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP0289936B1 (sv) |
| JP (1) | JP2671260B2 (sv) |
| KR (1) | KR970001235B1 (sv) |
| CN (1) | CN1029989C (sv) |
| AR (1) | AR241658A1 (sv) |
| AT (1) | ATE94905T1 (sv) |
| AU (1) | AU612144B2 (sv) |
| CA (1) | CA1338338C (sv) |
| DE (2) | DE3714866A1 (sv) |
| DK (1) | DK175525B1 (sv) |
| ES (1) | ES2045004T3 (sv) |
| FI (1) | FI97549C (sv) |
| HU (1) | HU204094B (sv) |
| IE (1) | IE62522B1 (sv) |
| IL (1) | IL86277A0 (sv) |
| NO (1) | NO178036C (sv) |
| NZ (1) | NZ224464A (sv) |
| PT (1) | PT87400B (sv) |
| ZA (1) | ZA883168B (sv) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5426036A (en) * | 1987-05-05 | 1995-06-20 | Hoechst Aktiengesellschaft | Processes for the preparation of foreign proteins in streptomycetes |
| DE4012818A1 (de) * | 1990-04-21 | 1991-10-24 | Hoechst Ag | Verfahren zur herstellung von fremdproteinen in streptomyceten |
| EP0367163B1 (de) * | 1988-11-03 | 1996-01-03 | Hoechst Aktiengesellschaft | Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten |
| DE3844211A1 (de) * | 1988-12-29 | 1990-07-05 | Hoechst Ag | Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung |
| DE3936876A1 (de) * | 1989-11-06 | 1991-05-23 | Hoechst Ag | Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3331860A1 (de) * | 1983-09-03 | 1985-03-21 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von tendamistat |
| DE3418274A1 (de) * | 1984-05-17 | 1985-11-21 | Hoechst Ag, 6230 Frankfurt | Signalpeptid fuer die exkretion von peptiden in streptomyceten |
| ATE63757T1 (de) * | 1985-03-28 | 1991-06-15 | Chiron Corp | Expression durch verwendung von fusionsgenen fuer proteinproduktion. |
| DE3707150A1 (de) * | 1987-03-06 | 1988-09-15 | Hoechst Ag | Tendamistat-derivate |
| EP0367163B1 (de) * | 1988-11-03 | 1996-01-03 | Hoechst Aktiengesellschaft | Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten |
-
1987
- 1987-05-05 DE DE19873714866 patent/DE3714866A1/de not_active Withdrawn
-
1988
- 1988-04-28 AT AT88106786T patent/ATE94905T1/de not_active IP Right Cessation
- 1988-04-28 ES ES88106786T patent/ES2045004T3/es not_active Expired - Lifetime
- 1988-04-28 DE DE88106786T patent/DE3884261D1/de not_active Expired - Lifetime
- 1988-04-28 EP EP88106786A patent/EP0289936B1/de not_active Expired - Lifetime
- 1988-05-03 NZ NZ224464A patent/NZ224464A/xx unknown
- 1988-05-03 KR KR1019880005131A patent/KR970001235B1/ko not_active Expired - Lifetime
- 1988-05-03 AR AR88310742A patent/AR241658A1/es active
- 1988-05-03 FI FI882059A patent/FI97549C/sv not_active IP Right Cessation
- 1988-05-04 HU HU882276A patent/HU204094B/hu unknown
- 1988-05-04 NO NO881942A patent/NO178036C/no unknown
- 1988-05-04 AU AU15559/88A patent/AU612144B2/en not_active Expired
- 1988-05-04 CA CA000565869A patent/CA1338338C/en not_active Expired - Lifetime
- 1988-05-04 IL IL86277A patent/IL86277A0/xx not_active IP Right Cessation
- 1988-05-04 DK DK198802416A patent/DK175525B1/da not_active IP Right Cessation
- 1988-05-04 IE IE133888A patent/IE62522B1/en not_active IP Right Cessation
- 1988-05-04 PT PT87400A patent/PT87400B/pt not_active IP Right Cessation
- 1988-05-04 ZA ZA883168A patent/ZA883168B/xx unknown
- 1988-05-04 CN CN88102568A patent/CN1029989C/zh not_active Expired - Lifetime
- 1988-05-06 JP JP63110295A patent/JP2671260B2/ja not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB | Publication of examined application | ||
| MM | Patent lapsed | ||
| MM | Patent lapsed |
Owner name: HOECHST AKTIENGESELLSCHAFT |