FI102539B1 - Nitriilihydrataasiaktiivisuutta omaavaa polypeptidiä koodaava DNA-fragmentti, geenin sisältävä transformantti ja menetelmä amidien tuottamiseksi transformanttia käyttäen - Google Patents
Nitriilihydrataasiaktiivisuutta omaavaa polypeptidiä koodaava DNA-fragmentti, geenin sisältävä transformantti ja menetelmä amidien tuottamiseksi transformanttia käyttäenInfo
- Publication number
- FI102539B1 FI102539B1 FI910960A FI910960A FI102539B1 FI 102539 B1 FI102539 B1 FI 102539B1 FI 910960 A FI910960 A FI 910960A FI 910960 A FI910960 A FI 910960A FI 102539 B1 FI102539 B1 FI 102539B1
- Authority
- FI
- Finland
- Prior art keywords
- transformant
- amides
- polypeptide
- gene
- dna fragment
- Prior art date
Links
- 150000001408 amides Chemical class 0.000 title abstract 4
- 150000002825 nitriles Chemical class 0.000 title abstract 3
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 230000000694 effects Effects 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 title abstract 2
- 102000004867 Hydro-Lyases Human genes 0.000 title 1
- 108090001042 Hydro-Lyases Proteins 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 108010024026 Nitrile hydratase Proteins 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 230000000887 hydrating effect Effects 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4807890 | 1990-02-28 | ||
| JP4807890 | 1990-02-28 | ||
| US2846393 | 1993-03-09 | ||
| US08/028,463 US5731176A (en) | 1990-02-28 | 1993-03-09 | DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI910960A0 FI910960A0 (fi) | 1991-02-27 |
| FI910960L FI910960L (fi) | 1991-08-29 |
| FI102539B1 true FI102539B1 (fi) | 1998-12-31 |
| FI102539B FI102539B (fi) | 1998-12-31 |
Family
ID=26388301
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI910960A FI102539B (fi) | 1990-02-28 | 1991-02-27 | Nitriilihydrataasiaktiivisuutta omaavaa polypeptidiä koodaava DNA-frag mentti, geenin sisältävä transformantti ja menetelmä amidien tuottamis eksi transformanttia käyttäen |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US5731176A (fi) |
| EP (1) | EP0445646B1 (fi) |
| JP (1) | JP3162091B2 (fi) |
| KR (1) | KR950002004B1 (fi) |
| CN (1) | CN1054639C (fi) |
| AT (1) | ATE138101T1 (fi) |
| AU (1) | AU627648B2 (fi) |
| CA (1) | CA2037291C (fi) |
| CZ (2) | CZ284017B6 (fi) |
| DE (1) | DE69119454T2 (fi) |
| DK (1) | DK0445646T3 (fi) |
| ES (1) | ES2089044T3 (fi) |
| FI (1) | FI102539B (fi) |
| MX (1) | MX193790B (fi) |
| NO (1) | NO306684B1 (fi) |
| PL (1) | PL166201B1 (fi) |
| RU (1) | RU2081173C1 (fi) |
| SK (1) | SK280199B6 (fi) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2907479B2 (ja) * | 1990-02-28 | 1999-06-21 | 輝彦 別府 | ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法 |
| DE69231939T2 (de) * | 1991-03-04 | 2002-04-04 | Teruhiko Beppu | Hybrid-Plasmid-Vektoren, die für Nitril-abbauende Enzyme kodierende Gene enthalten und Verfahren zur Herstellung von Amiden und Säuren |
| AU3692593A (en) * | 1992-04-28 | 1993-11-04 | Mitsubishi Kasei Corporation | Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene |
| JP3828590B2 (ja) * | 1994-10-03 | 2006-10-04 | 昌 清水 | ニトリルヒドラターゼ遺伝子発現のための調節遺伝子 |
| JP3154633B2 (ja) * | 1994-12-28 | 2001-04-09 | 三菱レイヨン株式会社 | ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子 |
| WO1997012964A2 (en) * | 1995-10-06 | 1997-04-10 | E.I. Du Pont De Nemours And Company | Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides |
| EP0790310B1 (en) * | 1996-02-14 | 2008-10-08 | Mitsui Chemicals, Inc. | Nitrile hydratase, derivatives thereof and methods of producing compounds |
| US5866379A (en) * | 1997-01-28 | 1999-02-02 | Novus International | Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha. |
| FR2770214B1 (fr) * | 1997-10-24 | 1999-12-31 | Rhodia Chimie Sa | Procede de preparation d'un benzamide halogene |
| JP2000050866A (ja) * | 1998-08-06 | 2000-02-22 | Rikagaku Kenkyusho | ニトリルヒドラターゼの生産方法 |
| JP4185607B2 (ja) | 1998-12-15 | 2008-11-26 | ダイセル化学工業株式会社 | 新規な微生物及びアミド化合物の製造方法 |
| RU2310686C2 (ru) * | 1999-06-25 | 2007-11-20 | Басф Акциенгезелльшафт | Гены corynebacterium glutamicum, кодирующие белки, участвующие в метаболизме углерода и продуцировании энергии |
| RU2306339C2 (ru) * | 1999-06-25 | 2007-09-20 | Басф Акциенгезелльшафт | ГЕНЫ Corynebacterium glutamicum, КОДИРУЮЩИЕ БЕЛКИ МЕТАБОЛИЧЕСКИХ ПУТЕЙ |
| RU2304616C2 (ru) * | 1999-06-25 | 2007-08-20 | Басф Акциенгезелльшафт | Гены corynebacterium glutamicum, кодирующие белки, участвующие в гомеостазе и адаптации |
| RU2312145C2 (ru) * | 1999-06-25 | 2007-12-10 | Басф Акциенгезелльшафт | Гены corynebacterium glutamicum, кодирующие белки, участвующие в синтезе мембран и мембранном транспорте |
| RU2303635C2 (ru) * | 1999-06-25 | 2007-07-27 | Басф Акциенгезелльшафт | Гены corynebacterium glutamicum, кодирующие белки резистентности и толерантности к стрессам |
| RU2326170C2 (ru) * | 1999-07-01 | 2008-06-10 | Басф Акциенгезелльшафт | Гены corynebacterium glutamicum, кодирующие белки системы фосфоенолпируват-сахар-фосфотрансферазы |
| KR100540991B1 (ko) | 1999-10-26 | 2006-01-10 | 쇼와 덴코 가부시키가이샤 | 신규한 로도코커스속 세균, 로도코커스속 세균 유래의 니트릴라아제 유전자, 니트릴 하이드라타아제 유전자 및 아미다아제 유전자, 및 이들을 이용한 카르복실산의 제조방법 |
| DE10010149A1 (de) * | 2000-03-03 | 2001-09-06 | Basf Ag | Nitrilase aus Rhodococcus rhodochrous NCIMB 11216 |
| DE60133130T2 (de) * | 2000-12-20 | 2009-02-26 | Dia-Nitrix Co.Ltd. | Verfahren zur herstellung einer amidverbindung unter verwendung eines mikrobiellen katalysators |
| KR100903756B1 (ko) * | 2001-01-09 | 2009-06-18 | 론자 아게 | 아미드의 미생물학적 제조 방법 |
| JP2002325587A (ja) | 2001-03-02 | 2002-11-12 | Daicel Chem Ind Ltd | ニトリルヒドラターゼ、およびアミドの製造方法 |
| JP2004194588A (ja) | 2002-12-19 | 2004-07-15 | Mitsui Chemicals Inc | 新規なニトリルヒドラターゼ |
| WO2004067738A2 (en) * | 2003-01-27 | 2004-08-12 | Degussa Ag | Nitrile hydratases from rhodococcus erythropolis and their application |
| ATE532859T1 (de) | 2003-12-02 | 2011-11-15 | Ciba Spec Chem Water Treat Ltd | Stamm von rhodococcus rhodochrous ncimb 41164 sowie dessen verwendung als nitrilhydrataseproduzent |
| JP4922757B2 (ja) * | 2004-05-26 | 2012-04-25 | 三菱レイヨン株式会社 | 改良型ニトリルヒドラターゼ |
| JP2006187257A (ja) | 2005-01-07 | 2006-07-20 | Daiyanitorikkusu Kk | アミド化合物の製造方法およびアクリルアミド系ポリマー |
| EP1842907A1 (en) | 2006-04-07 | 2007-10-10 | B.R.A.I.N. Ag | A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity |
| JP5120852B2 (ja) * | 2006-07-06 | 2013-01-16 | 国立大学法人 筑波大学 | 新規タンパク質複合体、該タンパク質複合体を用いたコバルト型低分子量ニトリルヒドラターゼ成熟化方法、成熟化コバルト型低分子量ニトリルヒドラターゼ、及び該ニトリルヒドラターゼを用いた方法 |
| US8334132B2 (en) | 2006-11-09 | 2012-12-18 | Mitsubishi Rayon Co., Ltd. | Process for production of a betaine such as carnitine |
| CN103649312B (zh) | 2011-05-31 | 2017-03-22 | 三菱丽阳株式会社 | 改良型腈水合酶 |
| WO2012169203A1 (ja) | 2011-06-07 | 2012-12-13 | ダイヤニトリックス株式会社 | 改良型ニトリルヒドラターゼ |
| FR3002542B1 (fr) * | 2013-02-28 | 2016-01-22 | Servier Lab | Procede de synthese enzymatique de l'acide (7s) 3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene 7-carboxylique et application a la synthese de l'ivabradine et de ses sels |
| WO2015186298A1 (ja) | 2014-06-06 | 2015-12-10 | 三菱レイヨン株式会社 | 改良型ニトリルヒドラターゼ |
| US11504418B2 (en) | 2016-11-04 | 2022-11-22 | Crowpierce Technologies, Llc | Endotoxin free asparaginase |
| CN116888269A (zh) | 2021-02-10 | 2023-10-13 | 三菱化学株式会社 | 用醛提高腈水合酶的反应性 |
| CN114934006A (zh) * | 2022-06-02 | 2022-08-23 | 无锡新晨宇生物工程有限公司 | 一种腈水合酶催化乙腈生成乙酰胺的应用 |
| EP4682261A1 (en) | 2023-03-17 | 2026-01-21 | Mitsubishi Chemical Corporation | Method for producing amide compound |
| AU2024239889A1 (en) | 2023-03-17 | 2025-09-18 | Mitsubishi Chemical Corporation | Method for producing amide compound |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD274631A5 (de) * | 1987-09-18 | 1989-12-27 | Kk | Verfahren zur biologischen herstellung von amiden |
| JP2840253B2 (ja) * | 1988-07-06 | 1998-12-24 | 輝彦 別府 | ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法 |
-
1991
- 1991-02-22 AU AU71293/91A patent/AU627648B2/en not_active Expired
- 1991-02-27 JP JP03323691A patent/JP3162091B2/ja not_active Expired - Lifetime
- 1991-02-27 EP EP91102937A patent/EP0445646B1/en not_active Expired - Lifetime
- 1991-02-27 AT AT91102937T patent/ATE138101T1/de not_active IP Right Cessation
- 1991-02-27 DK DK91102937.9T patent/DK0445646T3/da active
- 1991-02-27 MX MX9124722A patent/MX193790B/es unknown
- 1991-02-27 NO NO910797A patent/NO306684B1/no not_active IP Right Cessation
- 1991-02-27 FI FI910960A patent/FI102539B/fi active
- 1991-02-27 RU SU914894871A patent/RU2081173C1/ru active
- 1991-02-27 ES ES91102937T patent/ES2089044T3/es not_active Expired - Lifetime
- 1991-02-27 DE DE69119454T patent/DE69119454T2/de not_active Expired - Lifetime
- 1991-02-28 CZ CZ943170A patent/CZ284017B6/cs not_active IP Right Cessation
- 1991-02-28 CZ CS91531A patent/CZ284048B6/cs not_active IP Right Cessation
- 1991-02-28 CA CA002037291A patent/CA2037291C/en not_active Expired - Lifetime
- 1991-02-28 CN CN91101323A patent/CN1054639C/zh not_active Expired - Lifetime
- 1991-02-28 SK SK531-91A patent/SK280199B6/sk not_active IP Right Cessation
- 1991-02-28 PL PL91289239A patent/PL166201B1/pl unknown
- 1991-02-28 KR KR1019910003258A patent/KR950002004B1/ko not_active Expired - Lifetime
-
1993
- 1993-03-09 US US08/028,463 patent/US5731176A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CZ284017B6 (cs) | 1998-07-15 |
| CA2037291A1 (en) | 1991-08-29 |
| CN1054639C (zh) | 2000-07-19 |
| PL166201B1 (pl) | 1995-04-28 |
| FI910960L (fi) | 1991-08-29 |
| DE69119454T2 (de) | 1996-09-26 |
| CZ317094A3 (cs) | 1998-05-13 |
| AU7129391A (en) | 1991-09-12 |
| KR910021473A (ko) | 1991-12-20 |
| FI910960A0 (fi) | 1991-02-27 |
| ES2089044T3 (es) | 1996-10-01 |
| CN1054616A (zh) | 1991-09-18 |
| NO910797L (no) | 1991-08-29 |
| CS9100531A2 (en) | 1991-10-15 |
| DK0445646T3 (da) | 1996-09-30 |
| AU627648B2 (en) | 1992-08-27 |
| EP0445646B1 (en) | 1996-05-15 |
| JPH04211379A (ja) | 1992-08-03 |
| MX193790B (en) | 1999-10-19 |
| US5731176A (en) | 1998-03-24 |
| CZ284048B6 (cs) | 1998-07-15 |
| CA2037291C (en) | 2001-07-24 |
| SK280199B6 (sk) | 1999-09-10 |
| RU2081173C1 (ru) | 1997-06-10 |
| PL289239A1 (en) | 1992-03-23 |
| NO306684B1 (no) | 1999-12-06 |
| ATE138101T1 (de) | 1996-06-15 |
| EP0445646A3 (en) | 1992-01-08 |
| DE69119454D1 (de) | 1996-06-20 |
| JP3162091B2 (ja) | 2001-04-25 |
| KR950002004B1 (ko) | 1995-03-08 |
| EP0445646A2 (en) | 1991-09-11 |
| FI102539B (fi) | 1998-12-31 |
| NO910797D0 (no) | 1991-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GB | Transfer or assigment of application |
Owner name: MITSUBISHI RAYON CO., LTD |