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ES2163284T3 - Posicionamiento y caracterizacion electrofisiologica de celulas individuales y sistemas de membranas reconstituidos sobre soportes microestructurados. - Google Patents

Posicionamiento y caracterizacion electrofisiologica de celulas individuales y sistemas de membranas reconstituidos sobre soportes microestructurados.

Info

Publication number
ES2163284T3
ES2163284T3 ES98932469T ES98932469T ES2163284T3 ES 2163284 T3 ES2163284 T3 ES 2163284T3 ES 98932469 T ES98932469 T ES 98932469T ES 98932469 T ES98932469 T ES 98932469T ES 2163284 T3 ES2163284 T3 ES 2163284T3
Authority
ES
Spain
Prior art keywords
positioning
individual cells
membrane systems
electrophysiological characterization
reconstituted membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98932469T
Other languages
English (en)
Inventor
Horst Vogel
Christian Schmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Cytion SA
Original Assignee
ECOLE POLYTECH
Ecole Polytechnique Federale de Lausanne EPFL
Cytion SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=4244210&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2163284(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ECOLE POLYTECH, Ecole Polytechnique Federale de Lausanne EPFL, Cytion SA filed Critical ECOLE POLYTECH
Application granted granted Critical
Publication of ES2163284T3 publication Critical patent/ES2163284T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Disposición de medición con por lo menos dos electrodos (6, 9) y compartimentos separados aptos para la recepción de líquidos, caracterizada porque entre dos electrodos redox (6, 9) dispuestos uno enfrentado al otro y que se extienden hasta por lo menos un compartimento cada uno o que hacen contacto con un tal compartimento y presentan cualquier forma, se encuentra un soporte (1) eléctricamente aislante que separa por lo menos 2 compartimentos uno del otro y contiene por lo menos una abertura (3), en la que la abertura (3) une los compartimentos y tiene un diámetro tal que, al existir una diferencia de tensión por encima del soporte, mediada por los electrodos (6, 9), se genera un campo eléctrico no homogéneo alrededor de la abertura que aumenta en magnitud al acercarse a la abertura y es capaz de trasladar cerca de la abertura vesículas, células, fragmentos de células u organellos biológicos a la misma por electroforesis, lo cual permite un exacto posicionamiento y una unión eléctricamenteselladora de vesículas, células, fragmentos de células y otros organellos biológicos o membranas orgánicas de origen adecuado con la abertura.
ES98932469T 1997-12-17 1998-07-28 Posicionamiento y caracterizacion electrofisiologica de celulas individuales y sistemas de membranas reconstituidos sobre soportes microestructurados. Expired - Lifetime ES2163284T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH290397 1997-12-17

Publications (1)

Publication Number Publication Date
ES2163284T3 true ES2163284T3 (es) 2002-01-16

Family

ID=4244210

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98932469T Expired - Lifetime ES2163284T3 (es) 1997-12-17 1998-07-28 Posicionamiento y caracterizacion electrofisiologica de celulas individuales y sistemas de membranas reconstituidos sobre soportes microestructurados.

Country Status (10)

Country Link
US (3) US6758961B1 (es)
EP (1) EP1040349B2 (es)
JP (1) JP3486171B2 (es)
AT (1) ATE205300T1 (es)
AU (1) AU746580B2 (es)
CA (1) CA2316966C (es)
DE (1) DE59801410D1 (es)
DK (1) DK1040349T4 (es)
ES (1) ES2163284T3 (es)
WO (1) WO1999031503A1 (es)

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JP3486171B2 (ja) 2004-01-13
CA2316966A1 (en) 1999-06-24
EP1040349A1 (de) 2000-10-04
AU8237998A (en) 1999-07-05
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DK1040349T3 (da) 2002-01-28
HK1028450A1 (en) 2001-02-16
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CA2316966C (en) 2008-04-08
ATE205300T1 (de) 2001-09-15
US6758961B1 (en) 2004-07-06
EP1040349B2 (de) 2012-12-19
WO1999031503A1 (de) 1999-06-24
US20090153153A1 (en) 2009-06-18
US20020104757A1 (en) 2002-08-08
AU746580B2 (en) 2002-05-02

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