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ES2000278A6 - Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica. - Google Patents

Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica.

Info

Publication number
ES2000278A6
ES2000278A6 ES8600544A ES8600544A ES2000278A6 ES 2000278 A6 ES2000278 A6 ES 2000278A6 ES 8600544 A ES8600544 A ES 8600544A ES 8600544 A ES8600544 A ES 8600544A ES 2000278 A6 ES2000278 A6 ES 2000278A6
Authority
ES
Spain
Prior art keywords
sequence
amino acids
preparing
fusion protein
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8600544A
Other languages
English (en)
Inventor
Paul Habermann
Siegfried Stengelin
Friedrich Wengenmayer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES2000278A6 publication Critical patent/ES2000278A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

UN SEGMENTO DE APROXIMADAMENTE 70 AMINOACIDOS A PARTIR DE LA PROTEINA D DEL OPERON TRP DE E. COLI ES APROPIADO PARA LA CONSTRUCCION POR TECNOLOGIA GENETICA DE PROTEINAS DE FUSION, QUE PUEDEN CONTENER DELANTE DEL N-TERMINAL DE LA PROTEINA DESEADA UNA CORTA SECUENCIA DE AMINOACIDOS A BASE DE AMINOACIDOS CODIFICABLES GENETICAMENTE, LA CUAL ES PREFERIBLEMENTE LYS-ALA O CONTIENE N-TERMINALMENTE LYS-ALA.
ES8600544A 1985-07-27 1986-07-24 Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica. Expired ES2000278A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853526995 DE3526995A1 (de) 1985-07-27 1985-07-27 Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung

Publications (1)

Publication Number Publication Date
ES2000278A6 true ES2000278A6 (es) 1988-02-01

Family

ID=6276985

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8600544A Expired ES2000278A6 (es) 1985-07-27 1986-07-24 Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica.

Country Status (18)

Country Link
EP (1) EP0211299B1 (es)
JP (1) JPH07113040B2 (es)
KR (1) KR950000299B1 (es)
AT (1) ATE50596T1 (es)
AU (1) AU595221B2 (es)
CA (1) CA1336329C (es)
DE (2) DE3526995A1 (es)
DK (1) DK172618B1 (es)
ES (1) ES2000278A6 (es)
FI (1) FI86887C (es)
GR (1) GR861957B (es)
HU (1) HU197356B (es)
IE (1) IE59127B1 (es)
IL (1) IL79522A (es)
NO (1) NO175640C (es)
NZ (1) NZ216967A (es)
PT (1) PT83065B (es)
ZA (1) ZA865556B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
DE3541856A1 (de) * 1985-11-27 1987-06-04 Hoechst Ag Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
DE3781819T2 (de) * 1986-03-12 1993-01-07 Imcera Group Inc Hybride expressionsvektoren, deren herstellung und verwendungen.
WO1988010299A1 (en) * 1987-06-24 1988-12-29 Novo-Nordisk A/S A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
IL95495A (en) * 1989-08-29 1996-10-16 Hoechst Ag Fusion proteins their preparation and use
US5358857A (en) * 1989-08-29 1994-10-25 The General Hospital Corp. Method of preparing fusion proteins
US5227293A (en) * 1989-08-29 1993-07-13 The General Hospital Corporation Fusion proteins, their preparation and use
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
DE3942580A1 (de) * 1989-12-22 1991-06-27 Basf Ag Verfahren zur herstellung von hirudin
ES2218622T3 (es) 1996-07-26 2004-11-16 Aventis Pharma Deutschland Gmbh Derivados de insulina con actividad de union al zinc incrementada.
DE19726167B4 (de) 1997-06-20 2008-01-24 Sanofi-Aventis Deutschland Gmbh Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung
DE19825447A1 (de) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
DE10114178A1 (de) 2001-03-23 2002-10-10 Aventis Pharma Gmbh Zinkfreie und zinkarme Insulinzubereitungen mit verbesserter Stabilität
DE10227232A1 (de) 2002-06-18 2004-01-15 Aventis Pharma Deutschland Gmbh Saure Insulinzubereitungen mit verbesserter Stabilität
US8507221B2 (en) 2007-09-21 2013-08-13 Cadila Healthcare Limited Process for the expression of peptides of interest using GCSF as a fusion partner
DK2349324T3 (en) 2008-10-17 2017-12-11 Sanofi Aventis Deutschland COMBINATION OF AN INSULIN AND A GLP-1 AGONIST
KR101836070B1 (ko) 2009-11-13 2018-03-09 사노피-아벤티스 도이칠란트 게엠베하 Ｇｌｐ－１ 작용제, 인슐린 및 메티오닌을 포함하는 약제학적 조성물
ES2965209T3 (es) 2009-11-13 2024-04-11 Sanofi Aventis Deutschland Composición farmacéutica que comprende desPro36exendina-4(1-39)-Lys6-NH2 y metionina
CN103179978A (zh) 2010-08-30 2013-06-26 赛诺菲-安万特德国有限公司 Ave0010用于制造供治疗2型糖尿病用的药物的用途
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
ES2550357T3 (es) 2011-08-29 2015-11-06 Sanofi-Aventis Deutschland Gmbh Combinación farmacéutica para su uso en el control glucémico en pacientes de diabetes de tipo 2
TWI559929B (en) 2011-09-01 2016-12-01 Sanofi Aventis Deutschland Pharmaceutical composition for use in the treatment of a neurodegenerative disease
HRP20230470T1 (hr) 2014-12-12 2023-07-21 Sanofi-Aventis Deutschland Gmbh Formulacija fiksnog omjera inzulin glargin/liksisenatid
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
KR20200080748A (ko) 2018-12-27 2020-07-07 주식회사 폴루스 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
KR20200080747A (ko) 2018-12-27 2020-07-07 주식회사 폴루스 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
JPS62502586A (ja) * 1985-03-18 1987-10-08 ジ−ン　ラブズ，インコ−ポレイテツド ハイブリツド遺伝子カセツトベクタ−
GB8507666D0 (en) * 1985-03-25 1985-05-01 Wellcome Found Epidermal growth factor production
EP0220234B1 (en) * 1985-04-08 1994-08-03 Genetic Systems Corporation EXPRESSION AND DIAGNOSTIC USE OF gag ENCODED PEPTIDES WHICH ARE IMMUNOLOGICALLY REACTIVE WITH ANTIBODIES TO LAV

Also Published As

Publication number Publication date
EP0211299A3 (en) 1988-06-01
DK355486D0 (da) 1986-07-25
DK172618B1 (da) 1999-03-01
EP0211299B1 (de) 1990-02-28
HUT43629A (en) 1987-11-30
KR950000299B1 (ko) 1995-01-13
FI863041A0 (fi) 1986-07-24
JPH07113040B2 (ja) 1995-12-06
FI86887C (fi) 1992-10-26
NZ216967A (en) 1988-08-30
JPS6229600A (ja) 1987-02-07
PT83065B (pt) 1989-02-28
FI86887B (fi) 1992-07-15
CA1336329C (en) 1995-07-18
DE3526995A1 (de) 1987-02-05
IL79522A0 (en) 1986-10-31
IE59127B1 (en) 1994-01-12
DK355486A (da) 1987-01-28
IE861977L (en) 1987-01-27
ATE50596T1 (de) 1990-03-15
AU595221B2 (en) 1990-03-29
PT83065A (de) 1986-08-01
IL79522A (en) 1991-08-16
AU6056586A (en) 1987-01-29
EP0211299A2 (de) 1987-02-25
FI863041L (fi) 1987-01-28
NO863000D0 (no) 1986-07-25
ZA865556B (en) 1987-03-25
DE3669175D1 (de) 1990-04-05
NO175640C (no) 1994-11-09
KR870001312A (ko) 1987-03-13
NO863000L (no) 1987-01-28
GR861957B (en) 1986-11-25
NO175640B (es) 1994-08-01
HU197356B (en) 1989-03-28

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20060725