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EP3198019A1 - Acides aminés essentiels fournis par bacillus dans l'alimentation liquide - Google Patents

Acides aminés essentiels fournis par bacillus dans l'alimentation liquide

Info

Publication number
EP3198019A1
EP3198019A1 EP15756153.1A EP15756153A EP3198019A1 EP 3198019 A1 EP3198019 A1 EP 3198019A1 EP 15756153 A EP15756153 A EP 15756153A EP 3198019 A1 EP3198019 A1 EP 3198019A1
Authority
EP
European Patent Office
Prior art keywords
liquid feed
bacillus
feed
dsm
hours
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP15756153.1A
Other languages
German (de)
English (en)
Inventor
Beatrice Nielsen
Mette Dines Cantor
Patrick Derkx
Hanne Damgaard POULSEN
Bent Borg Jensen
Jan Vaerum NOERGAARD
Nuria CANIBE
Karoline BLAABJERG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chr Hansen AS
Original Assignee
Chr Hansen AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chr Hansen AS filed Critical Chr Hansen AS
Publication of EP3198019A1 publication Critical patent/EP3198019A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids

Definitions

  • TITLE Essential amino acids provided by Bacillus in liquid feed
  • Liquid feed on farms is prepared by mixing feed, additives and water in a tank before feeding to the animals. Some farms ferment the feed before feeding to the animals to improve the feed quality and the gastro-intestinal balance of the animals. Typically the feed is fermented by naturally occurring or added lactic acid bacteria producing lactic acid to reduce the pH which will kill pathogenic microorganisms that often have good growth conditions in liquid feed .
  • liquid feed is typically prepared in a tank and then pumped out through a pipeline (pipelines) to the different pens where the animals are housed .
  • a pipeline pipelines
  • Such a system typically includes many pipelines to ensure the supply to all animals. In most systems some liquid feed will stay in the pipelines until the next feeding .
  • the present invention relates to inoculation of liquid feed with at least one Bacillus strain capable of producing a high amount of essential amino acids during a fermentation process by synthesizing these amino acids or by degrading proteins to amino acids by protease activity.
  • Amino acid production by Bacillus can be used in both fermented and non-fermented liquid feed .
  • non-fermented feed Bacillus will grow and produce amino acids in the feed left in the pipelines in the period from one feeding to the next feeding .
  • high protein content in the feed also involves loss of nitrogen as the animals cannot utilize all the nitrogen supplied to them. Typically only 40% of the supplied protein is utilized while 60% is excreted through the urine and feces. This may result in nitrogen wash out to lakes and streams with subsequent negative effects on animals and groundwater due to oxygen depletion.
  • high protein content in the feed may cause a higher risk for diarrhea, higher consumption of antibiotics as well as a high feed price and thus poor economy for the farmer.
  • different amino acids are considered as essential.
  • amino acids in general regarded as essential are phenylalanine, valine, threonine, tryptophan, isoleucine, methionine, leucine, lysine, tyrosine and histidine.
  • amino acids arginine, cysteine (or sulphur-containing amino acids), gly- cine, glutamine, proline and serine are considered essential for some animal species.
  • Essential amino acids are "essential" not because they are more important to life than the others, but because the animal species does not de novo synthesize them and consequently they must be added with the diet to secure optimal farm animal performance.
  • Provision of available limiting essential amino acids would make it possible to formulate feed rations with reduced protein content.
  • a higher provision of available limiting amino acids would result in reduced nitrogen loss to the environment, improved production economy, healthier animals and subsequently, a reduced use of antibiot- ics.
  • further use of essential amino acids is not possible due to the very high cost of the amino acids and the fact that only a few of the essential amino acids are produced synthetically and thus are commercially available.
  • Adding synthetic amino acids to the diet may also have application issues as many amino acids have to be sprayed onto the feed pellets after the heating process. The spraying process results in higher cost of the feed manufacturing process.
  • the problem to be solved by the present invention is to provide a sufficient amount of essential amino acids in liquid feed.
  • the solution is a process for increasing the amount of at least one essential amino acid in liquid feed, the process comprising inoculating a liquid feed with a Bacillus strain, which is capable of producing at least one essential amino acid in an amount of at least 600 mg/kg dry matter (DM) of at least one of the essential amino acids lysine, methionine, threonine and valine and/or at least 200 mg/kg dry matter (DM) of at least one of the essential amino acids tryptophan, isoleucine, histidine, phenylalanine, leucine, tyrosine, arginine, glycine, glutamine, proline and serine, when inoculated into a liquid feed, which is fermented at a temperature of at least 30°C under good aerobic conditions for 24 hours.
  • the invention relates to novel Bacillus strains which are capable of supplying at least one essential amino acid when
  • the present invention is based upon the surprising finding that certain Bacillus strains can produce a high amount of at least one essential amino acid when inoculated into a liquid feed .
  • Liquid feed has advantages compared to dry feed due to several reasons : 1) the use of cheap liquid by-products from several sources makes liquid feed more economical for the farmer, 2) reduced environmental load due to the (re)use of resources such as liquid by-products is an advantage for the society, and 3) improved animal health including reduced number of pathogens in the GIT resulting in better animal produc- tion and reduced antibiotic use is an advantage for as well the farmer, the society and the animals.
  • liquid feeding increases digestibility of phytate bound phosphorus leading to reduced pollution (Lyberg K et al ., 2006 and Blaabjerg, K et al ., 2010) .
  • weaned piglets can take advantage from liquid feed as they do not need to get used to dry feed because the liquid feed is similar to sow milk in consistence and their high feed intake can thereby be ensured .
  • Liquid feed can be based on cereals such as corn, barley or wheat, protein rich raw materials such as soy bean meal, rape seed meal, fish meal or dried distiller grains solubles (DDGS) as well as different additives such as organic acids, probiotics, amino acids etc.
  • DDGS distiller grains solubles
  • Different byproducts from the grain- , animal- , vegetable - or sugar and starch industry can be included such as i .e. bakery waste, liquid whey, corn glu- ten meal, and wheat bran . These are only examples and other ingredients can be included in the liquid feed as well .
  • liquid feed By the term “liquid feed” is meant feed ingredients such as barley, corn and soybean meal, premixes or additives such as organic acids, vitamins and amino acids mixed together with water.
  • Liquid feed may refer to a feed with a high water content (e.g ., 2 : 1 watenfeed) .
  • Liquid feed can be fed at once to the animals or it can be fermented for a given period before being fed to the animals.
  • a high amount of at least one essential amino acid is meant that the amount of a given amino acid has increased with at least 600 mg/kg dry matter (DM) of at least one of the essential amino acids lysine, methionine, threonine and valine and/or at least 200 mg/kg dry matter (DM) of at least one of the essential amino acids tryptophan, isoleucine, histidine, phenylalanine, leucine, tyrosine, arginine, glycine, glutamine, proline and serine when inoculated into a liquid feed which is fer- mented at a temperature of at least 30°C under good aerobic conditions for 24 hours and measured by HPLC or GC-MS.
  • the strain is able to produce an even higher amount such as at least 900 mg/kg dry matter (DM) of at least one of the essential amino acids lysine, methio- nine, threonine and valine, preferably at least 1200 mg/kg dry matter (DM) of at least one of the essential amino acids lysine, methionine, threonine and valine.
  • DM dry matter
  • the strain is able to produce more than one, such as two, three or four of the amino acids in an amount of at least 600 mg/kg dry matter (DM) .
  • the amount of a given amino acid may increase by at least 200 mg/kg/dry matter (DM), such as at least 300 mg/kg, preferably at least 400 mg/kg, of at least one of the essential amino acids tryptophan, isoleucine, histidine, phenylalanine, leucine, tyrosine, arginine, glycine, glutamine, proline and/or serine when inoculated into a liquid feed which is fermented at a temperature of at least 30°C under good aerobic conditions for 24 hours.
  • DM dry matter
  • the strain is able to produce more than one, such as two, three, four, five, six, seven, eight, nine, ten, or eleven of these amino acids in an amount of at least 200 mg/kg dry matter (DM) .
  • Some strains according to the invention are able to produce amino acids from both lists.
  • the term "inoculation”, as used herein, refers to the act of introducing microorganisms or a suspension of microorganisms (e.g . Bacillus) into a culture medium (e.g . liquid feed) .
  • a liquid fermentation starter culture is a microbiological culture which performs fermentation .
  • a starter culture usually consists of a cultivation medium, such as grains, seeds, or nutrient liquids, which has been well colonized by microorganisms used for the fermentation .
  • Some starter cultures include various enzymes in addition to mi- croorganisms.
  • Another example of inoculation of culture medium (e.g . liquid feed) is the addition of dried microorganisms (e.g . spray dried) cells, powder, granula or the addition of frozen cells.
  • a starter culture comprising one or more Bacillus may be used for the inoculation of the liquid feed .
  • the starter culture is incubated with or without stirring at an appropriate temperature (e.g . from 15°C - 37°C) for a sufficient period of time (e.g . at least 4 hours and no more than 7 days) before being mixed with an appropriate amount of non-fermented feed and fed to the farm animals.
  • the incubation will last for a period of at least 8, 12, 18, 24, 48, or 72 hours.
  • the temperature may be as low as 10°C, it is more likely to be at least 25°C, such as at least 30 °C, e.g . 37°C.
  • a mixture of fermented (e.g . the starter culture) and non-fermented feed may be incubated with or without stirring at an appropriate temperature (e.g . from 15-25°C) for a sufficient period of time (e.g . more than 2 hours and less than 2 days) before being fed to the animals.
  • an appropriate temperature e.g . from 15-25°C
  • a sufficient period of time e.g . more than 2 hours and less than 2 days
  • the process comprises the steps of
  • Bacillus cells exist as bacillus spore cells and bacillus vegetative cells. When reference is made herein to Bacillus cells, this relates to both.
  • Bacillus spore in relation to a Bacillus spore cell relates herein to a spore that according to the art may be characterized as a dormant, tough, non- reproductive structure produced by Bacillus bacteria.
  • the primary function of spores is generally to ensure the survival of a bacterium through periods of environmental stress. They are therefore resistant to ultraviolet and gamma radiation, desiccation, lysozyme, temperature, starvation, and chemical disinfectants. Spores are commonly found in soil and water, where they may survive for long periods of time.
  • the spore coat is impermeable to many toxic molecules and may also contain enzymes that are involved in germination.
  • the core has normal cell structures, such as DNA and ribo- somes, but is metabolically inactive. When a bacterium detects that environmental conditions are becoming unfavorable it may start the process of sporulation, which takes about eight hours.
  • Bacillus vegetative cell relates to functional vegetative Bacillus cells, which can divide to produce more vegetative cells.
  • the strain of the invention is of the genus Bacillus, preferably one of the species Bacillus amyloliquefaciens, such as Bacillus amyloliquefaciens subsp. amyloliquefaciens or Bacillus amyloliquefaciens subsp.
  • Bacillus simplex Bacillus licheniform- is, Bacillus megaterium, Bacillus mojavensis, Bacillus pumilus, Bacillus safensis, Bacillus simplex, Bacillus subtilis, Bacillus atrophaeus, Bacillus lentus, Bacillus methylotrophicus, Bacillus siamensis, Bacillus vallismortis or Bacillus tequilensis.
  • a bacterial "strain” as used herein refers to a bacterium which remains genetically unchanged when grown or multiplied. The multiplicity of identical bacteria are included.
  • Wild type strain refers to the non-mutated form of a bacterium, as found in nature. Such Bacillus cells have not been under any selective pressure to provide a high amount of amino acids neither by synthesis nor degradation of proteins when inoculated into liquid feed. It is therefore believed that "wild-type" Bacillus cells do not provide a high amount of amino acids.
  • a “mutant bacterium” or a “mutant strain” refers to a natural (spontaneous, naturally occurring) mutant bacterium or an induced mutant bacterium comprising one or more mutations in its genome (DNA) which are absent in the wild type DNA.
  • an "induced mutant” is a bacterium where the mutation was induced by human treatment, such as treatment with any conventionally used mutagenization treatment including treatment with chemical mutagens, such as a chemical mutagen selected from (i) a mutagen that associates with or become incorporated into DNA such as a base analogue, e.g . 2-aminopurine or an interchelating agent such as ICR-191, (ii) a mutagen that reacts with the DNA including alkylating agents such as nitrosoguanidine or hy- droxylamine, or ethane methyl sulphonate (EMS) or N-methyl-N'-nitro-N- nitroguanidine (NTG), UV- or gamma radiation etc.
  • a "spontaneous mutant” or "naturally occurring mutant” has not been mutagenized by man.
  • a mutant may have been subjected to several mutagenization treatments (a single treatment should be understood one mutagenization step followed by a screening/selection step), but it is presently preferred that no more than 20, or no more than 10, or no more than 5, treatments (or screening/selection steps) are carried out.
  • no more than 20, or no more than 10, or no more than 5, treatments (or screening/selection steps) are carried out.
  • less than 1%, less than 0.1, less than 0.01, less than 0.001% or even less than 0.0001% of the nucleotides in the bacterial genome have been replaced with another nucleotide, or deleted, compared to the mother strain.
  • Mutant bacteria as described above are non-GMO, i .e. not modified by recombinant DNA technology.
  • the mutant When the mutant is provided as a spontaneously occurring mutant the above wild- type strain is subjected to the selection step without any preceding mutagenization treatment.
  • Bacillus subtilis deposited as DSM 25483 can produce the amino acid valine in a liquid feed resulting in a production of 250 mg/kg liquid feed with 16% dry matter corresponding to 1560 mg/kg feed dry matter. This amount corresponds to an amino acid content in the liquid feed of 1.5%. This amount is significant compared to the amount needed in the pig feed industry for growers and piglets (0.1%). Both liquid feed based on barley as well as standard feed with cereals and protein sources have been tested with good results.
  • DSM 26668 and DSM 26669 which are also developed as mutants of DSM 19467 and which have been found to provide a significant amount of valine and tryptophan, respectively, when inoculated into liquid feed, as well as with other mutants developed from DSM 19467 or the mother strain of this strain, DSM 17231.
  • DSM 26668, DSM 26669 and DSM 25483 have structural genomic changes compared to DSM 19467.
  • Genomic DNA was prepared from the four strains and DNA was sequenced using an Illumina HiSeq platform. The genome sequences were analyzed using the CLC genome workbench (CLC Bio, Arhus, Denmark) by reference assembly of reads from one strain towards de novo assembly of another strain and lists of mutations or DNA sequence variations also called Single Nucleotide Polymorphism (SNP's) were generated.
  • DSM 25483 and DSM 26668 had 5 and 7 unique SNP ' s respectively compared to the mother strain DSM 19467 and 2 in non-coding regions.
  • DSM 26669 had 3 unique SNP compared to the mother strain DSM 19467 in a non- coding region.
  • a mutant strain of the Bacillus subtilis strains with accession numbers DSM 25483, DSM 26668, or DSM 26669 can be obtained by subjecting the strain to mutageniza- tion treatment as described to obtain mutant strains and selecting for mutant strains having the desired properties. Alternatively, a selection is performed for spontaneously occurring mutants.
  • the invention relates to a Bacillus composition com- prising cells of a Bacillus strain of the invention.
  • the composition may comprise cells of at least one, at least two, at least three, at least four or even more Bacillus strains chosen from at least one of the strains of the invention.
  • the cells of the Bacillus composition are spore cells.
  • the relevant Bacillus strains of the composition may be present in a commercially relevant form known to the skilled person. Accordingly, in an embodiment the Bacillus strains of the composition are present as dried (e.g. spray dried) cells or as frozen cells.
  • the composition may be provided in any suitable form such as in the form of a liquid, a slurry, a powder or a pellet.
  • the Bacillus composition comprises from 10 5 to 10 12 CFU/g, more preferably from 10 5 to 10 12 CFU/g, and most preferably from 10 7 to 10 12 CFU/g .
  • CFU/g relates to the gram weight of the composition as such, including suitable relevant additives present in the composition .
  • a commercially relevant bacterial composition generally also comprises other relevant additives such as e.g . one carrier/ingredient of the group belonging to whey, whey permeate, calcium carbonate/limestone and anti caking agents such as aluminum silicates and kieselgur (diatomaceous earth) . It does not include the weight of a suitable container used to package the Bacillus composition.
  • One embodiment of the invention relates to a composition packaged into a suitable container.
  • Compositions of the present invention may include a Bacillus strain of the invention including mutants, and carriers that make these compositions suitable for addition to animals' feed .
  • the Bacillus strain of the invention including mutants may be formulated with ingredients for liquid feed, including feed protein and/or feed carbohydrates. Such combinations may be in the form of pellets that are extruded through standard pelleting processes.
  • the invention also provides a method for producing a liquid feed or premix comprising adding a Bacillus composition of the invention to an animal feed, and appropriate instructions for fermenting the liquid feed .
  • the invention relates to a Bacillus composition
  • a Bacillus composition comprising cells of at least one Bacillus strain.
  • the composition may comprise cells of at least one, at least two, at least three, at least four or even more Bacillus strains. If the composition comprises more than one strain, each of the strains may be present as 5%, 10%, 20%, 25%, 33%, 40%, 50%, 60%, 66%, 75%, 80%, 90% or 95% of the Bacillus cells.
  • premix refers to a Bacillus strain added to a carrier to make a premix which is then added to the feed at a desired inclusion rate and fed to the animal .
  • the invention further relates to a method of feeding animals comprising the steps of mixing a liquid feed and a Bacillus composition, premix or starter culture as described above, fermenting said mixture at 15°C to 37°C for 1 to 96 hours, and feed- ing said mixture to an animal, wherein the fermented liquid feed gains an elevated level of at least one essential amino acid .
  • the animal may be selected from the group consisting of poultry, ruminants, calves, pigs, rabbits, horses, fish and pets.
  • the animal is a farm animal, which is raised for consumption, such as pigs, broilers and turkey, or as food-producers, such as layers.
  • a sample of the novel Bacillus subtilis strain BacTRP has been deposited at DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Maschroder Weg lb, D-38124 Braunschweig) under the accession number DSM 26669 with a deposit date of November 27, 2012. The deposit has been made under the conditions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.
  • DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Maschroder Weg lb, D-38124 Braunschweig
  • TSA Trypic-soy agar
  • Colistinsulfat (Sigma, C4461)
  • Rapeseed meal 0 10.00 %
  • Soybean meal 9.80 5.00 %
  • Swinevit 448 (vitamins & minerals) 0.20 0.20 %
  • Vitamin Bl 2.10 2.10 mg
  • Vitamin B2 2.10 2.10 mg
  • Vitamin B6 (3a831) 3.15 3.15 mg
  • Vitamin D3 (E671) 420 420 IE
  • a fermentation of liguid feed was done in 4 small lab-scale bioreactors of 2 liters.
  • the bioreactor containing the pH and oxygen probes were added 1250 ml tap water, the probes were calibrated and the whole system sterilized by steam sterilization at 121 °C. After the temperature was stable at 37°C 250 gram feed and 0.085% anti- foam were added.
  • the feed was used as Is (without sterilization).
  • the dissolved oxy- gen concentration was adjusted to 100% air saturation and the fermentations were initiated for experiment 1 in the 4 bioreactors with I) negative control feed classic, II) feed classic + DSM 25483, III) negative control feed ideal and IV) feed ideal + DSM 25483.
  • DSM 25483 was added at a concentration of 0.7 E+07 CFU/g.
  • the fermentations were carried out at 37°C at a stirring rate of 500 rpm with continuous air sparking (500 ml/min) where pH and dissolved oxygen could be continuously determined by use of pH and oxygen probes.
  • Vegetative cells and spores were analyzed by spread plate technique using trypic-soy agar (TSA) added colistine to kill other bacteria. Spores were analyzed by spread plate technique using TSA and heat treatment at 80°C for 10 minutes to kill other bacteria.
  • TSA trypic-soy agar
  • the concentrations of the amino acids valine, lysine, methionine, and threonine in the fermentation broth were measured by HPLC as described by Lazanov et al., 2007.

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  • General Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

Cette invention concerne un procédé permettant d'accroître la quantité d'au moins un acide aminé essentiel dans une charge liquide, le procédé comprenant l'inoculation d'une souche de Bacillus dans une charge liquide et la charge liquide obtenue par ledit procédé ainsi que des souches de Bacillus subtilis spécifiques appropriées à ces fins.
EP15756153.1A 2014-08-29 2015-08-27 Acides aminés essentiels fournis par bacillus dans l'alimentation liquide Withdrawn EP3198019A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201400485 2014-08-29
PCT/EP2015/069592 WO2016030441A1 (fr) 2014-08-29 2015-08-27 Acides aminés essentiels fournis par bacillus dans une charge liquide

Publications (1)

Publication Number Publication Date
EP3198019A1 true EP3198019A1 (fr) 2017-08-02

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EP15756153.1A Withdrawn EP3198019A1 (fr) 2014-08-29 2015-08-27 Acides aminés essentiels fournis par bacillus dans l'alimentation liquide

Country Status (3)

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EP (1) EP3198019A1 (fr)
CA (1) CA2958602A1 (fr)
WO (1) WO2016030441A1 (fr)

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US10201574B1 (en) 2015-09-16 2019-02-12 Church & Dwight Co., Inc. Methods of microbial treatment of poultry
US11298383B2 (en) 2016-05-20 2022-04-12 Church & Dwight Co., Inc. Lactobacillus and bacillus based direct fed microbial treatment for poultry and method of use
EP3464648A1 (fr) 2016-05-25 2019-04-10 Church & Dwight Co., Inc. Compositions de bacillus et méthodes d'utilisation avec les ruminants
CN107217018B (zh) * 2017-06-19 2019-10-25 武汉轻工大学 高抗性枯草芽孢杆菌及其应用
US11622569B2 (en) 2017-07-24 2023-04-11 Church & Dwight Co., Inc. Bacillus microbial terroir for pathogen control in swine
CN109517867B (zh) * 2018-11-26 2021-10-15 中国农业科学院油料作物研究所 一种极端酸、碱水解法在芽孢杆菌发酵油料粕生产伊枯草菌素a中的应用及其方法
CN114196574B (zh) * 2021-11-30 2025-03-14 南京农业大学 产α-生育酚的特基拉芽孢杆菌QH1及其应用
CN115918909B (zh) * 2022-12-30 2023-10-17 西南科技大学 一种猪源芽孢杆菌在制备抗氧化和提升免疫的制品中的应用

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DE102006026328A1 (de) * 2006-06-02 2008-01-03 Evonik Degussa Gmbh Verfahren zur Herstellung eines L-Lysin enthaltenden Futtermitteladditivs
RU2015135354A (ru) * 2008-12-19 2018-12-26 Кр. Хансен А/С Композиция палочковидных бактерий, устойчивых к желчи, секретирующих высокие уровни незаменимых аминокислот
JP2012512639A (ja) * 2008-12-19 2012-06-07 セーホーエル．ハンセン アクティーゼルスカブ 胆汁耐性バチルス組成物

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CA2958602A1 (fr) 2016-03-03
WO2016030441A1 (fr) 2016-03-03

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