DE60233734D1 - Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs - Google Patents

Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs

Info

Publication number: DE60233734D1
Authority: DE; Germany
Prior art keywords: glp; colibracillus; genes; promoting; glucagon
Prior art date: 2001-07-19
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE60233734T

Other languages

English (en)

Inventor

Yukun Sun

Dengxi Wu

Aizhen Wu

Zhiyong Zhu

Gang Yu

Jiaxiang Zhou

Shaoling Zhao

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

SHANGHAI HUA-YI BIO-TECH LAB

SHANGHAI HUA YI BIO TECH LAB

Original Assignee

SHANGHAI HUA-YI BIO-TECH LAB

SHANGHAI HUA YI BIO TECH LAB

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-07-19

Filing date

2002-07-17

Publication date

2009-10-29

2002-07-17 Application filed by SHANGHAI HUA-YI BIO-TECH LAB, SHANGHAI HUA YI BIO TECH LAB filed Critical SHANGHAI HUA-YI BIO-TECH LAB

2009-10-29 Application granted granted Critical

2009-10-29 Publication of DE60233734D1 publication Critical patent/DE60233734D1/de

2022-07-18 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

DTHNMHAUYICORS-KTKZVXAJSA-N Glucagon-like peptide 1 Chemical class C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1N=CNC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 DTHNMHAUYICORS-KTKZVXAJSA-N 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
OBMZMSLWNNWEJA-XNCRXQDQSA-N C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 Chemical compound C1=CC=2C(C[C@@H]3NC(=O)[C@@H](NC(=O)[C@H](NC(=O)N(CC#CCN(CCCC[C@H](NC(=O)[C@@H](CC4=CC=CC=C4)NC3=O)C(=O)N)CC=C)NC(=O)[C@@H](N)C)CC3=CNC4=C3C=CC=C4)C)=CNC=2C=C1 OBMZMSLWNNWEJA-XNCRXQDQSA-N 0.000 title 1
101800000224 Glucagon-like peptide 1 Proteins 0.000 title 1
101710176384 Peptide 1 Proteins 0.000 title 1
238000004519 manufacturing process Methods 0.000 title 1
101800004295 Glucagon-like peptide 1(7-36) Proteins 0.000 abstract 5
241000894006 Bacteria Species 0.000 abstract 3
239000013612 plasmid Substances 0.000 abstract 3
108090000623 proteins and genes Proteins 0.000 abstract 3
NGJOFQZEYQGZMB-KTKZVXAJSA-N (4S)-5-[[2-[[(2S,3R)-1-[[(2S)-1-[[(2S,3R)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[2-[[(2S)-5-amino-1-[[(2S)-1-[[(2S)-1-[[(2S)-6-amino-1-[[(2S)-1-[[(2S)-1-[[(2S,3S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-6-amino-1-[[2-[[(1S)-4-carbamimidamido-1-carboxybutyl]amino]-2-oxoethyl]amino]-1-oxohexan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-methyl-1-oxopentan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-4-carboxy-1-oxobutan-2-yl]amino]-1-oxohexan-2-yl]amino]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-2-oxoethyl]amino]-4-carboxy-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-3-carboxy-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-2-oxoethyl]amino]-4-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-4-yl)propanoyl]amino]propanoyl]amino]-5-oxopentanoic acid Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 NGJOFQZEYQGZMB-KTKZVXAJSA-N 0.000 abstract 2
210000004027 cell Anatomy 0.000 abstract 2
229940088597 hormone Drugs 0.000 abstract 2
239000005556 hormone Substances 0.000 abstract 2
230000002473 insulinotropic effect Effects 0.000 abstract 2
230000001737 promoting effect Effects 0.000 abstract 2
230000028327 secretion Effects 0.000 abstract 2
102400000325 Glucagon-like peptide 1(7-36) Human genes 0.000 abstract 1
238000010367 cloning Methods 0.000 abstract 1
238000005336 cracking Methods 0.000 abstract 1
108020001507 fusion proteins Proteins 0.000 abstract 1
102000037865 fusion proteins Human genes 0.000 abstract 1
239000001963 growth medium Substances 0.000 abstract 1
210000003000 inclusion body Anatomy 0.000 abstract 1
238000009630 liquid culture Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biomedical Technology (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

DE60233734T 2001-07-19 2002-07-17 Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs Expired - Lifetime DE60233734D1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
CN01126278A CN1363654A (zh)	2001-07-19	2001-07-19	生产促胰岛素分泌肽glp－1(7－36)的基因工程菌以及生产glp－1(7－36)的方法
PCT/CN2002/000502 WO2003016349A1 (fr)	2001-07-19	2002-07-17	Methode de production d'un peptide 1 de type glucagon (glp-1) 7-36 et d'un analogue de glp-1

Publications (1)

Publication Number	Publication Date
DE60233734D1 true DE60233734D1 (de)	2009-10-29

Family

ID=4666300

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE60233734T Expired - Lifetime DE60233734D1 (de)	2001-07-19	2002-07-17	Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs

Country Status (13)

Country	Link
US (1)	US7544512B2 (de)
EP (1)	EP1408050B1 (de)
JP (1)	JP4504014B2 (de)
KR (1)	KR100959549B1 (de)
CN (2)	CN1363654A (de)
AT (1)	ATE443081T1 (de)
AU (1)	AU2002313869B2 (de)
BR (1)	BR0211435B1 (de)
CA (1)	CA2454264C (de)
DE (1)	DE60233734D1 (de)
DK (1)	DK1408050T3 (de)
ES (1)	ES2333413T3 (de)
WO (1)	WO2003016349A1 (de)

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CN1162446C (zh)	2001-05-10	2004-08-18	上海华谊生物技术有限公司	促胰岛素分泌肽衍生物
US8129504B2 (en)	2001-08-30	2012-03-06	Biorexis Technology, Inc.	Oral delivery of modified transferrin fusion proteins
US7176278B2 (en)	2001-08-30	2007-02-13	Biorexis Technology, Inc.	Modified transferrin fusion proteins
EP1626981A4 (de) *	2003-03-04	2006-11-22	Biorexis Pharmaceutical Corp	Dipeptidylpeptidase-geschützte proteine
CN100392070C (zh) *	2004-09-08	2008-06-04	华东师范大学	表达人胰高血糖素样肽-1的基因工程菌及其构建方法和应用
US7947876B2 (en)	2004-09-14	2011-05-24	Nippon Paper Industries Co., Ltd.	Plant and plant storage organ having GLP-1 derivative accumulated therein and method of producing the same
EP1799710A2 (de) *	2004-10-07	2007-06-27	Novo Nordisk A/S	Glp-1 verbindungen mit verlängerter wirkdauer
JP5107713B2 (ja) *	2004-10-07	2012-12-26	ノヴォノルディスクアー／エス	遅延性のエキセンディン−４化合物
ES2397289T3 (es) *	2005-09-22	2013-03-06	Biocompatibles Uk Ltd.	Polipéptidos de fusión de GLP-1 (péptido 1 de tipo glucagón) con resistencia a peptidasa incrementada
HRP20160866T1 (hr) *	2005-11-04	2016-10-07	Glaxosmithkline Llc	Postupci primjene hipoglikemijskih sredstava
EP1854455B1 (de) *	2006-05-10	2009-10-07	Biocompatibles UK Limited	GLP-1 Peptide enthaltende kugelförmige Mikrokapseln, deren Produktion und deren Verwendung
PT2038423E (pt) *	2006-06-21	2013-03-27	Biocon Ltd	Método de produção de um polipéptido biologicamente activo possuindo actividade insulinotrópica
WO2008011446A2 (en)	2006-07-18	2008-01-24	Centocor, Inc.	Human glp-1 mimetibodies, compositions, methods and uses
CA2658654A1 (en)	2006-07-24	2008-01-31	Biorexis Pharmaceutical Corporation	Exendin fusion proteins
EP1972349A1 (de) *	2007-03-21	2008-09-24	Biocompatibles UK Limited	Mit Polymer(en) konjugierte GLP-1-Fusionspeptide, ihre Herstellung und Verwendung
EP1975176A1 (de) *	2007-03-27	2008-10-01	Biocompatibles UK Limited	Neue GLP-1-Fusionspeptide, ihre Herstellung und Verwendung
CN101220088B (zh) *	2007-09-24	2012-01-25	中国人民解放军第四军医大学	人胰高血糖素样肽-1类似物的融合蛋白及其应用
EP2163243A1 (de) *	2008-09-12	2010-03-17	Biocompatibles UK Limited	Behandlung akuter Herzinfarkte mithilfe eingekapselter Zellen, welche GLP-1-Peptide oder deren Analoga kodieren und absondern
CN102093475B (zh) *	2009-12-11	2014-02-26	吴晓琰	胰高血糖素样肽-1的类似物
CN102242127B (zh) *	2011-05-20	2012-12-12	严杰	串联抗菌肽Alloferon-1的制备方法及rAlloferon-1-K的应用
KR20140148420A (ko) *	2012-03-19	2014-12-31	마들렌 파마수티컬스 피티와이 리미티드	재조합 펩티드의 생산 방법
CN107586330B (zh) *	2016-07-08	2021-04-16	上海多米瑞生物技术有限公司	替度鲁肽串联多肽及替度鲁肽制备方法
KR102700388B1 (ko) *	2019-02-24	2024-08-30	온코시미스 바이오테크 프라이빗 리미티드	박테리아에 의한 재조합 glp-1 펩타이드의 연속 생성 방법
CA3224490A1 (en) *	2021-06-30	2023-01-05	Allysta Pharmaceuticals, Inc.	Monomeric fusion peptides and method of use thereof

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JPH06306100A (ja) *	1993-04-26	1994-11-01	Sanwa Kagaku Kenkyusho Co Ltd	Ｖｉｐアナログ調製用融合蛋白、ｖｉｐアナログの製法並びにそのための遺伝子組換えプラスミド及び形質転換微生物
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DE69916811T2 (de) *	1998-02-27	2005-04-14	Novo Nordisk A/S	Glp-1 derivate mit einem helix-gehalt über 25 %, die partiell strukturierte mizellenartige aggregate bilden
WO1999043705A1 (en) *	1998-02-27	1999-09-02	Novo Nordisk A/S	N-terminally truncated glp-1 derivatives
JP4716641B2 (ja)	2000-06-16	2011-07-06	イーライリリーアンドカンパニー	グルカゴン様ペプチド−１類似体

2001
- 2001-07-19 CN CN01126278A patent/CN1363654A/zh active Pending
2002
- 2002-07-17 AT AT02752955T patent/ATE443081T1/de not_active IP Right Cessation
- 2002-07-17 WO PCT/CN2002/000502 patent/WO2003016349A1/zh not_active Ceased
- 2002-07-17 DE DE60233734T patent/DE60233734D1/de not_active Expired - Lifetime
- 2002-07-17 BR BRPI0211435-6A patent/BR0211435B1/pt active IP Right Grant
- 2002-07-17 AU AU2002313869A patent/AU2002313869B2/en not_active Expired
- 2002-07-17 CN CN02814355A patent/CN100579986C/zh not_active Expired - Lifetime
- 2002-07-17 ES ES02752955T patent/ES2333413T3/es not_active Expired - Lifetime
- 2002-07-17 JP JP2003521271A patent/JP4504014B2/ja not_active Expired - Lifetime
- 2002-07-17 DK DK02752955.1T patent/DK1408050T3/da active
- 2002-07-17 CA CA2454264A patent/CA2454264C/en not_active Expired - Lifetime
- 2002-07-17 EP EP02752955A patent/EP1408050B1/de not_active Expired - Lifetime
- 2002-07-17 KR KR1020047000825A patent/KR100959549B1/ko not_active Expired - Lifetime
2004
- 2004-01-20 US US10/761,717 patent/US7544512B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
US7544512B2 (en)	2009-06-09
ES2333413T3 (es)	2010-02-22
AU2002313869B2 (en)	2008-04-03
BR0211435B1 (pt)	2014-12-23
WO2003016349A1 (fr)	2003-02-27
ATE443081T1 (de)	2009-10-15
CA2454264C (en)	2010-06-22
CA2454264A1 (en)	2003-02-27
JP2005515167A (ja)	2005-05-26
EP1408050B1 (de)	2009-09-16
US20040146985A1 (en)	2004-07-29
CN1363654A (zh)	2002-08-14
KR100959549B1 (ko)	2010-05-27
KR20040097114A (ko)	2004-11-17
DK1408050T3 (da)	2010-01-18
EP1408050A1 (de)	2004-04-14
EP1408050A4 (de)	2005-08-10
BR0211435A (pt)	2004-07-13
CN100579986C (zh)	2010-01-13
CN1531551A (zh)	2004-09-22
JP4504014B2 (ja)	2010-07-14

Publication	Publication Date	Title
DE60233734D1 (de)	2009-10-29	Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs
Marston	1986	The purification of eukaryotic polypeptides synthesized in Escherichia coli
EP1865064B1 (de)	2011-09-07	Verfahren zur Expression kleiner Peptide über nichtspeichernde Getreideproteine als endosperme Fusionsträger und ihre Verwendung
JP2021003117A (ja)	2021-01-14	ペプチド産生のための融合パートナー
Clausmeyer et al.	1993	Protein import into chloroplasts. The hydrophilic lumenal proteins exhibit unexpected import and sorting specificities in spite of structurally conserved transit peptides
JPH04502861A (ja)	1992-05-28	植物および植物細胞中での非相同タンパク類の生産
JP2010516758A5 (de)	2011-04-21
IL172469A (en)	2011-08-31	Methods for expression of insulin in plant seeds and such seeds
RU2006145020A (ru)	2008-06-27	Полипептиды и конъюгаты интерферона-альфа
Finch et al.	1986	Complete nucleotide sequence of the Escherichia coti recC gene and of the thyA-recC intergenk region
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