DE60117754D1 - Verfahren zur herstellung von rekombinantem trypsin - Google Patents
Verfahren zur herstellung von rekombinantem trypsinInfo
- Publication number
- DE60117754D1 DE60117754D1 DE60117754T DE60117754T DE60117754D1 DE 60117754 D1 DE60117754 D1 DE 60117754D1 DE 60117754 T DE60117754 T DE 60117754T DE 60117754 T DE60117754 T DE 60117754T DE 60117754 D1 DE60117754 D1 DE 60117754D1
- Authority
- DE
- Germany
- Prior art keywords
- recombinant
- trypsine
- preparation
- trypsinogen
- relates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 102000018690 Trypsinogen Human genes 0.000 abstract 2
- 108010027252 Trypsinogen Proteins 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 102000004142 Trypsin Human genes 0.000 abstract 1
- 108090000631 Trypsin Proteins 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 210000003000 inclusion body Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000030788 protein refolding Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 239000012588 trypsin Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17734800P | 2000-01-24 | 2000-01-24 | |
| US177348P | 2000-01-24 | ||
| PCT/EP2001/000770 WO2001055429A2 (en) | 2000-01-24 | 2001-01-24 | Method for the manufacture of recombinant trypsin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60117754D1 true DE60117754D1 (de) | 2006-05-04 |
| DE60117754T2 DE60117754T2 (de) | 2006-11-16 |
Family
ID=22648255
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60117754T Expired - Lifetime DE60117754T2 (de) | 2000-01-24 | 2001-01-24 | Verfahren zur herstellung von rekombinantem trypsin |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1250442B1 (de) |
| AT (1) | ATE319837T1 (de) |
| AU (1) | AU2001240540A1 (de) |
| DE (1) | DE60117754T2 (de) |
| WO (1) | WO2001055429A2 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4159878B2 (ja) | 2001-02-01 | 2008-10-01 | エフ．ホフマン−ラ ロシュ アーゲー | 組換えトリプシンの製造方法 |
| AU2002239151A1 (en) * | 2002-03-08 | 2003-09-22 | Crucell Holland B.V. | Use of recombinant trypsin for vaccine production |
| US20040043455A1 (en) | 2002-08-30 | 2004-03-04 | Novozymes Biotech, Inc. | Methods for producing mammalian trypsins |
| CN101967469B (zh) * | 2009-07-28 | 2012-11-14 | 上海雅心生物技术有限公司 | 一种高稳定性的重组胰蛋白酶的生产及应用 |
| WO2012104099A1 (en) * | 2011-02-04 | 2012-08-09 | Glucometrix Ag | Process for the production of recombinant trypsin |
| CN110511916B (zh) * | 2019-08-06 | 2021-06-01 | 杭州浦泰生物科技有限公司 | 一种重组胰蛋白酶的生产工艺 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0833898A1 (de) * | 1995-06-16 | 1998-04-08 | Novo Nordisk A/S | Verfahren zur herstellung von trypsin |
-
2001
- 2001-01-24 AT AT01911524T patent/ATE319837T1/de active
- 2001-01-24 DE DE60117754T patent/DE60117754T2/de not_active Expired - Lifetime
- 2001-01-24 AU AU2001240540A patent/AU2001240540A1/en not_active Abandoned
- 2001-01-24 EP EP01911524A patent/EP1250442B1/de not_active Expired - Lifetime
- 2001-01-24 WO PCT/EP2001/000770 patent/WO2001055429A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1250442A2 (de) | 2002-10-23 |
| AU2001240540A1 (en) | 2001-08-07 |
| WO2001055429A3 (en) | 2002-05-16 |
| DE60117754T2 (de) | 2006-11-16 |
| WO2001055429A2 (en) | 2001-08-02 |
| EP1250442B1 (de) | 2006-03-08 |
| ATE319837T1 (de) | 2006-03-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |