[go: up one dir, main page]

AU2001240540A1 - Method for the manufacture of recombinant trypsin - Google Patents

Method for the manufacture of recombinant trypsin

Info

Publication number
AU2001240540A1
AU2001240540A1 AU2001240540A AU4054001A AU2001240540A1 AU 2001240540 A1 AU2001240540 A1 AU 2001240540A1 AU 2001240540 A AU2001240540 A AU 2001240540A AU 4054001 A AU4054001 A AU 4054001A AU 2001240540 A1 AU2001240540 A1 AU 2001240540A1
Authority
AU
Australia
Prior art keywords
manufacture
trypsinogen
recombinant trypsin
trypsin
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001240540A
Inventor
Hubertus Hohenblum
Hermann Katinger
Diethard Mattanovich
Stefan Naschberger
Robert Weik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Polymun Scientific Immunbiologische Forschung GmbH
Original Assignee
Polymun Scientific Immunbiologische Forschung GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Polymun Scientific Immunbiologische Forschung GmbH filed Critical Polymun Scientific Immunbiologische Forschung GmbH
Publication of AU2001240540A1 publication Critical patent/AU2001240540A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a method for the manufacture andpurification of recombinant trypsinogen and trypsin in E. coli and yeast, using high yield expression vectors with and without secretion leader sequences. The invention further relates to an improved method and apparatus for carrying out protein refolding specifically useful for processing trypsinogen that has accumulated intracellularly in the form of inclusion bodies.
AU2001240540A 2000-01-24 2001-01-24 Method for the manufacture of recombinant trypsin Abandoned AU2001240540A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US17734800P 2000-01-24 2000-01-24
US60177348 2000-01-24
PCT/EP2001/000770 WO2001055429A2 (en) 2000-01-24 2001-01-24 Method for the manufacture of recombinant trypsin

Publications (1)

Publication Number Publication Date
AU2001240540A1 true AU2001240540A1 (en) 2001-08-07

Family

ID=22648255

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001240540A Abandoned AU2001240540A1 (en) 2000-01-24 2001-01-24 Method for the manufacture of recombinant trypsin

Country Status (5)

Country Link
EP (1) EP1250442B1 (en)
AT (1) ATE319837T1 (en)
AU (1) AU2001240540A1 (en)
DE (1) DE60117754T2 (en)
WO (1) WO2001055429A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1399568B1 (en) 2001-02-01 2005-11-16 Roche Diagnostics GmbH Method for producing recombinant trypsin
AU2002239151A1 (en) * 2002-03-08 2003-09-22 Crucell Holland B.V. Use of recombinant trypsin for vaccine production
CN1694953B (en) 2002-08-30 2010-05-12 诺维信股份有限公司 Method for preparing mammal trypsin
CN101967469B (en) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 Production and application of high stability recombinant trypsin
WO2012104099A1 (en) * 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN110511916B (en) * 2019-08-06 2021-06-01 杭州浦泰生物科技有限公司 Production process of recombinant trypsin

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997000316A1 (en) * 1995-06-16 1997-01-03 Novo Nordisk A/S A process for producing trypsin (trypsinogen)

Also Published As

Publication number Publication date
WO2001055429A2 (en) 2001-08-02
WO2001055429A3 (en) 2002-05-16
EP1250442A2 (en) 2002-10-23
DE60117754D1 (en) 2006-05-04
EP1250442B1 (en) 2006-03-08
ATE319837T1 (en) 2006-03-15
DE60117754T2 (en) 2006-11-16

Similar Documents

Publication Publication Date Title
IL157414A0 (en) Fusion protein for the secretion of a protein of interest into the supernatant of the bacterial culture
IL139478A0 (en) Hybrid protein
DK1204738T3 (en) Process for reducing foam formation during cultivation of a microorganism
WO2003010288A3 (en) Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
AU3944002A (en) Metabolic rate shifts in fermentations expressing recombinant proteins
DE60331224D1 (en) SUBTILISIN CARLSBERG PROTEINS WITH REDUCED IMMUNOGENICITY
AU2003222717A1 (en) Streptavidin-binding peptide
IL175021A0 (en) Chromatin insulator, its preparation and use in protein expression
AU2001240540A1 (en) Method for the manufacture of recombinant trypsin
IT1250692B (en) PROCEDURE FOR THE PREPARATION OF DEMETYLEPIPODOPHYLOTOXY-BETA-D-GLUCOSIDES.
WO2004056850A3 (en) Mutant proteins showing increased secretion
ITMI930456A1 (en) RECOMBINANT VECTOR AND ITS USE FOR THE EXOCELLULAR PREPARATION OF ANTIBODIES IN THE FORM OF A SINGLE MOLECULE FROM BACILLUS SUBTILIS
EP1250439A4 (en) EXPRESSION AND SECRETION VECTOR FOR THE HUMAN INTERPERON ALPHA AND PROCESS FOR OBTAINING THE HUMAN INTERFERON ALPHA USING THE SAME
AU2002345213A1 (en) Expression vectors encoding bacteriophage signal peptides
NO20053033D0 (en) Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions.
SG185137A1 (en) Biologically active peptides
AU2003202817A1 (en) Manufacturing method of recombinant protein in yeast by the use of secretory type vector
WO2003004599A3 (en) Methods of making and using expression constructs for the expression of recombinant proteins
WO2002036154A3 (en) Procollagen (iii) propeptides and related substances for treating fibrotic diseases
WO2004089973A3 (en) Peptides, polypeptides, and proteins of reduced immunogenicity and methods for their production
ZA969692B (en) Pulmonary surfactant peptide solubilization process.
AU2002348573A1 (en) Method of increasing expression of heterologous proteins in plants
AU2001278512A1 (en) Method for the production of imidazo-(1,2-c)(2,3)-benzodiazepines and intermediates in the production thereof
DK1290194T3 (en) Preparation of secreted polypeptides
AU2001292273A1 (en) Novel proteins, genes encoding them and method of using the same