[go: up one dir, main page]

DE60020340D1 - Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür - Google Patents

Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür

Info

Publication number
DE60020340D1
DE60020340D1 DE60020340T DE60020340T DE60020340D1 DE 60020340 D1 DE60020340 D1 DE 60020340D1 DE 60020340 T DE60020340 T DE 60020340T DE 60020340 T DE60020340 T DE 60020340T DE 60020340 D1 DE60020340 D1 DE 60020340D1
Authority
DE
Germany
Prior art keywords
dna sequences
long dna
synthesis procedures
compositions therefor
economic structure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60020340T
Other languages
English (en)
Other versions
DE60020340T2 (de
Inventor
M Brennan
L Heyneker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CODA GENOMICS,INC., LAGUNA HILLS, CALIF., US
Original Assignee
Metrigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metrigen Inc filed Critical Metrigen Inc
Application granted granted Critical
Publication of DE60020340D1 publication Critical patent/DE60020340D1/de
Publication of DE60020340T2 publication Critical patent/DE60020340T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60020340T 1999-03-08 2000-03-07 Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür Expired - Fee Related DE60020340T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US264388 1994-06-23
US26438899A 1999-03-08 1999-03-08
PCT/US2000/006335 WO2000053617A1 (en) 1999-03-08 2000-03-07 Methods and compositions for economically synthesizing and assembling long dna sequences

Publications (2)

Publication Number Publication Date
DE60020340D1 true DE60020340D1 (de) 2005-06-30
DE60020340T2 DE60020340T2 (de) 2006-03-16

Family

ID=23005849

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60020340T Expired - Fee Related DE60020340T2 (de) 1999-03-08 2000-03-07 Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür

Country Status (7)

Country Link
US (2) US20030186226A1 (de)
EP (1) EP1159285B1 (de)
JP (1) JP2002538790A (de)
AT (1) ATE296310T1 (de)
AU (1) AU3623800A (de)
DE (1) DE60020340T2 (de)
WO (1) WO2000053617A1 (de)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589726B1 (en) * 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
JP4503828B2 (ja) * 1998-02-11 2010-07-14 ユニバーシティー オブ ヒューストン 光生成試薬を用いる化学反応および生化学反応のための方法および装置
JP2002525590A (ja) 1998-08-28 2002-08-13 フェビット フェラリウス バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング 分析物測定法のための支持体および該支持体を製造する方法
ATE456652T1 (de) 1999-02-19 2010-02-15 Febit Holding Gmbh Verfahren zur herstellung von polymeren
JP2003519495A (ja) * 2000-01-11 2003-06-24 マキシジェン， インコーポレイテッド 多様性生成およびスクリーニングのための一体化されたシステムおよび方法
US7211654B2 (en) * 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
IL158487A0 (en) 2001-05-18 2004-05-12 Wisconsin Alumni Res Found Method for the synthesis of dna sequences
US10539561B1 (en) 2001-08-30 2020-01-21 Customarray, Inc. Enzyme-amplified redox microarray detection process
WO2003033718A1 (en) * 2001-10-17 2003-04-24 Global Genomics Ab Synthesis of oligonucleotides on solid support and assembly into doublestranded polynucleotides
EP1483409B1 (de) * 2002-02-28 2009-04-15 Wisconsin Alumni Research Foundation Verfahren zur fehlerreduzierung in nukleinsäurepopulationen
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7871799B2 (en) * 2002-11-22 2011-01-18 Lawrence Livermore National Security, Llc Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
CA2522637C (en) 2003-04-17 2014-01-21 Alnylam Pharmaceuticals, Inc. Modified irna agents
US7723509B2 (en) * 2003-04-17 2010-05-25 Alnylam Pharmaceuticals IRNA agents with biocleavable tethers
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
EP1544296B8 (de) * 2003-12-18 2009-08-05 Biomethodes Verfahren zur ortspezifischen Massenmutagenese
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
EP1733055A4 (de) * 2004-02-27 2009-03-11 Harvard College Polynukleotidsynthese
WO2005088310A2 (en) * 2004-03-05 2005-09-22 The Scripps Research Institute High throughput glycan microarrays
JP2008504531A (ja) * 2004-06-24 2008-02-14 ザ スクリップス リサーチ インスティテュート 切断可能なリンカーを有するアレイ
US20060008833A1 (en) * 2004-07-12 2006-01-12 Jacobson Joseph M Method for long, error-reduced DNA synthesis
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
CA2584984A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20060102471A1 (en) 2004-11-18 2006-05-18 Karl Maurer Electrode array device having an adsorbed porous reaction layer
WO2006068758A2 (en) * 2004-11-19 2006-06-29 The Scripps Research Institute Detection, prevention and treatment of breast cancer
US20060234264A1 (en) * 2005-03-14 2006-10-19 Affymetrix, Inc. Multiplex polynucleotide synthesis
US20070034513A1 (en) 2005-03-25 2007-02-15 Combimatrix Corporation Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
US9394167B2 (en) 2005-04-15 2016-07-19 Customarray, Inc. Neutralization and containment of redox species produced by circumferential electrodes
WO2007005053A1 (en) * 2005-06-30 2007-01-11 Codon Devices, Inc. Hierarchical assembly methods for genome engineering
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US20070065877A1 (en) 2005-09-19 2007-03-22 Combimatrix Corporation Microarray having a base cleavable succinate linker
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20070265170A1 (en) * 2006-05-15 2007-11-15 Ola Blixt Detection, prevention and treatment of ovarian cancer
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US20090036660A1 (en) * 2007-07-31 2009-02-05 Joel Myerson Methods and compositions for generating mixtures of nucleic acid molecules
US20090075840A1 (en) * 2007-09-18 2009-03-19 Joel Myerson Methods And Compositions For Generating Mixtures Of Nucleic Acid Molecules
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
EP2394165A4 (de) * 2009-02-03 2013-12-11 Complete Genomics Inc Zuordnung von oligomersequenzen
EP2394164A4 (de) * 2009-02-03 2014-01-08 Complete Genomics Inc Zuordnung von oligomersequenzen
US8738296B2 (en) * 2009-02-03 2014-05-27 Complete Genomics, Inc. Indexing a reference sequence for oligomer sequence mapping
WO2010127045A2 (en) * 2009-04-29 2010-11-04 Complete Genomics, Inc. Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP3597771A1 (de) 2009-11-25 2020-01-22 Gen9, Inc. Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2011090793A2 (en) 2010-01-20 2011-07-28 Customarray, Inc. Multiplex microarray of serially deposited biomolecules on a microarray
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
US8865404B2 (en) 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
EP4039363A1 (de) 2010-11-12 2022-08-10 Gen9, Inc. Proteinanordnungen und verfahren zu ihrer herstellung und verwendung
US9295965B2 (en) 2010-11-12 2016-03-29 Gen9, Inc. Methods and devices for nucleic acid synthesis
PT105960A (pt) * 2010-12-07 2012-08-16 Ass For The Advancement Of Tissue Engineering Cell Based Technologies And Therapies A4Tec Associacao Processo para deposição de biomateriais em substratos repelentes a água e resultantes biomateriais
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
IL302248A (en) 2011-08-26 2023-06-01 Gen9 Inc Compositions and methods for high fidelity assembly of nucleic acids
EP2766498B1 (de) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
LT2841601T (lt) 2012-04-24 2019-07-10 Gen9, Inc. Nukleorūgščių rūšiavimo būdai ir multipleksinis preparatyvinis in vitro klonavimas
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
IL236303B (en) * 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
EP3030682B1 (de) 2013-08-05 2020-06-03 Twist Bioscience Corporation De-novo-synthetisierte genbanken
US10726942B2 (en) 2013-08-23 2020-07-28 Complete Genomics, Inc. Long fragment de novo assembly using short reads
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EA201890763A1 (ru) 2015-09-18 2018-08-31 Твист Байосайенс Корпорейшн Библиотеки вариантных олигонуклеиновых кислот и их синтез
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CA3006867A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
WO2018026920A1 (en) * 2016-08-03 2018-02-08 Twist Bioscience Corporation Textured surfaces for polynucleotide synthesis
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
KR102217487B1 (ko) 2016-09-21 2021-02-23 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
CA3047128A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3066744A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2019051501A1 (en) 2017-09-11 2019-03-14 Twist Bioscience Corporation PROTEINS BINDING TO GPCR AND METHODS OF SYNTHESIS
KR102889470B1 (ko) 2017-10-20 2025-11-21 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
US10936953B2 (en) 2018-01-04 2021-03-02 Twist Bioscience Corporation DNA-based digital information storage with sidewall electrodes
KR20210013128A (ko) 2018-05-18 2021-02-03 트위스트 바이오사이언스 코포레이션 핵산 하이브리드화를 위한 폴리뉴클레오타이드, 시약 및 방법
CA3106410A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
EP3902816A4 (de) 2018-12-26 2022-09-14 Twist Bioscience Corporation Hochgenaue de-novo-polynukleotidsynthese
SG11202106445RA (en) 2019-01-03 2021-07-29 Dna Script One pot synthesis of sets of oligonucleotides
US11492727B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
AU2020227802A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US20220403434A1 (en) 2019-07-30 2022-12-22 Dna Script Sas Template-Free Enzymatic Synthesis of Polynucleotides Using Poly(A) and Poly(U) Polymerases
EP4007816A1 (de) 2019-08-01 2022-06-08 DNA Script Erhöhung der ausbeute von langen sequenzen bei der schablonenfreien enzymatischen synthese von polynukleotiden
CN115003697A (zh) 2019-09-23 2022-09-02 特韦斯特生物科学公司 Crth2的变异核酸文库
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
CA3151411A1 (en) 2019-09-23 2021-04-01 Adrian Horgan Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
US20230089448A1 (en) 2020-02-25 2023-03-23 Dna Script Method And Apparatus for Enzymatic Synthesis of Polynucleotides
WO2021178210A1 (en) 2020-03-03 2021-09-10 Codex Dna, Inc. Methods for assembling nucleic acids
EP4139446A1 (de) 2020-04-20 2023-03-01 DNA Script Terminale deoxynucleotidyltransferasevarianten und verwendungen davon
EP4142739A2 (de) 2020-04-27 2023-03-08 Twist Bioscience Corporation Varianten von nukleinsäurebibliotheken für coronavirus
EP4164784B1 (de) 2020-06-16 2025-06-25 DNA Script Verfahren zur enzymatischen polynukleotidsynthese
EP4182472A1 (de) 2020-07-15 2023-05-24 DNA Script Massiv parallele enzymatische synthese von polynukleotiden
CA3190667A1 (en) 2020-08-26 2022-03-03 Aaron Sato Methods and compositions relating to glp1r variants
WO2022047076A1 (en) 2020-08-28 2022-03-03 Twist Bioscience Corporation Devices and methods for synthesis
AU2021347452A1 (en) 2020-09-22 2023-04-27 Dna Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
WO2022086866A1 (en) 2020-10-19 2022-04-28 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
CA3203805A1 (en) 2021-01-08 2022-07-14 Cellanome, Inc. Devices and methods for analyzing biological samples
EP4281112A4 (de) 2021-01-21 2025-01-15 Twist Bioscience Corporation Verfahren und zusammensetzungen in verbindung mit adenosinrezeptoren
US12258406B2 (en) 2021-03-24 2025-03-25 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon
CN117222744A (zh) 2021-04-02 2023-12-12 Dna斯克瑞普特公司 用于酶法合成g4-易发的多核苷酸的方法和试剂盒
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
EP4395933A1 (de) 2021-09-03 2024-07-10 Elegen Corporation Vorrichtungen, systeme und verfahren zur mehrweg-wulstsortierung unter verwendung von druckquellen
US12325739B2 (en) 2022-01-03 2025-06-10 Twist Bioscience Corporation Bispecific SARS-CoV-2 antibodies and methods of use
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
EP4489910A1 (de) 2022-03-11 2025-01-15 DNA Script Automatisierungsstation für die enzymatische polynukleotidsynthese
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
EP4642920A1 (de) 2022-12-31 2025-11-05 DNA Script Tinten mit variabler viskosität zur tintenstrahlabgabe von enzymreagenzien
EP4652292A1 (de) 2023-01-16 2025-11-26 DNA Script Tintenstrahlunterstützte enzymatische nukleinsäuresynthese
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
WO2025019586A1 (en) 2023-07-18 2025-01-23 Twist Bioscience Corporation Spatially controlled data transfer
WO2025072571A1 (en) 2023-09-27 2025-04-03 Cellanome, Inc. Cell culture within microfluidic structures

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4744796A (en) * 1986-02-04 1988-05-17 Arco Chemical Company Microemulsion fuel system
US5641648A (en) * 1986-11-04 1997-06-24 Protein Polymer Technologies, Inc. Methods for preparing synthetic repetitive DNA
US5132215A (en) * 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6589726B1 (en) * 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US6921636B1 (en) * 1991-09-04 2005-07-26 Metrigen, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
EP1086742B2 (de) * 1991-11-22 2007-03-14 Affymetrix, Inc. (a Delaware Corporation) Kombinatorische Strategien für Polymersynthese
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
CA2193228A1 (en) * 1994-06-23 1996-01-04 Christopher Holmes Photolabile compounds and methods for their use
US6558633B1 (en) * 1994-09-21 2003-05-06 Isis Pharmaceuticals, Inc. Chemical reaction apparatus and methods
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5843655A (en) * 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US6083762A (en) * 1996-05-31 2000-07-04 Packard Instruments Company Microvolume liquid handling system
DE19736591A1 (de) * 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Also Published As

Publication number Publication date
US20030186226A1 (en) 2003-10-02
US20030068643A1 (en) 2003-04-10
WO2000053617A1 (en) 2000-09-14
AU3623800A (en) 2000-09-28
EP1159285A1 (de) 2001-12-05
DE60020340T2 (de) 2006-03-16
ATE296310T1 (de) 2005-06-15
EP1159285B1 (de) 2005-05-25
JP2002538790A (ja) 2002-11-19

Similar Documents

Publication Publication Date Title
DE60020340D1 (de) Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
BR0307432B1 (pt) difluormetiltiazolilcarboxanilidas
DE69835357D1 (de) Verfahren zur Herstellung von Synthesegas
YU27304A (sh) Novi dihidropteridinoni, postupak za njihovo dobijanje i njihova primena kao lekova
ID29538A (id) SENYAWA LOGAM TRANSISI, KATALIS UNTUK PRODUKSI α-OLEFIN-OLEFIN DAN PROSES UNTUK MEMPRODUKSI α-OLEFIN-OLEFIN
NO20021593D0 (no) Fremgangsmåte for fremstilling av höy aktivitets karbonmonoksidhydrogeneringskatalysator;katalysatorsammensetningene, anvendelse av katalysatorene for åutföre slike reaksjoner og produktene av slike reaksjoner
ATE302786T1 (de) Verfahren zur herstllung von clarithromycin polymorphen und polymorph iv
DE60009910D1 (de) Verfahren zur herstellung von zsm-5 und zsm-11
DE69813792T2 (de) Zwischenprodukte und verfahren zu ihrer herstellung
NO986116D0 (no) Forbedret metode for fremstilling av katalysator bestÕende av metaller av h
FI20011889A7 (fi) Menetelmä ksylitolin valmistamiseksi
AU2003248647A8 (en) Stereoselective synthesis of 1,2-disubstituted cycloalkyls
ATE457972T1 (de) Verfahren zur herstellung von 7-amino-syn-3,5- dihydroxyheptansäurederivaten und zwischenverbindungen diesesverfahrens und deren herestellung
EA200100040A1 (ru) Химический синтез морфолиновых производных
EA200100213A1 (ru) Способ синтеза производных (1h)-бензо[c]хинолизин-3-онов
ATE278702T1 (de) Verfahren zur synthese von phosphorothioat- oligonukelotiden
ATE281434T1 (de) Verfahren zur herstellung von benzoperhydroisoindolen
HUP0100943A2 (hu) Eljárás poliamidok előállítására
DE60106057D1 (de) Verfahren zur herstellung von baccatin iii-derivativen
ATE223414T1 (de) Prozess zur herstellung von zwischebprodukten für imidazodeazepinen
DE69621463D1 (de) Verfahren zum ausgewähltem schutz von baccatinderivaten und ihre verwendung für die synthese von taxanen
BG104073A (en) Method for preparing alkyloxy furanone derivatives, compounds obtained by said method and use of said compounds
ATE244706T1 (de) Enantiomere von mercaptolactonen und verfahren zu ihrer herstellung
ATE300534T1 (de) Verfahren und zwischenverbindungen zur herstellung von imidazopyridinen
EP1270582A4 (de) Triphenylphosphin-derivate, verfahren zu deren herstellung, diese enthaltender palladium-komplex und verfahren zur herstellung von biaryl-derivaten

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: CODA GENOMICS,INC., LAGUNA HILLS, CALIF., US

8339 Ceased/non-payment of the annual fee