CO5241325A1 - Procedimiento de cultivo masivo de celulas de mamifero para la obtencion de eritropoyetina humana recombinante y la eritropoyetina humana recombinante obtenida con tal procedimiento - Google Patents
Procedimiento de cultivo masivo de celulas de mamifero para la obtencion de eritropoyetina humana recombinante y la eritropoyetina humana recombinante obtenida con tal procedimientoInfo
- Publication number
- CO5241325A1 CO5241325A1 CO99069963A CO99069963A CO5241325A1 CO 5241325 A1 CO5241325 A1 CO 5241325A1 CO 99069963 A CO99069963 A CO 99069963A CO 99069963 A CO99069963 A CO 99069963A CO 5241325 A1 CO5241325 A1 CO 5241325A1
- Authority
- CO
- Colombia
- Prior art keywords
- erythropoyetin
- recombinant human
- procedure
- massifull
- mammer
- Prior art date
Links
- 102000003951 Erythropoietin Human genes 0.000 abstract 3
- 108090000394 Erythropoietin Proteins 0.000 abstract 3
- 229940105423 erythropoietin Drugs 0.000 abstract 3
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 abstract 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 101000987586 Homo sapiens Eosinophil peroxidase Proteins 0.000 abstract 1
- 101000920686 Homo sapiens Erythropoietin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 102000044890 human EPO Human genes 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Habiendo descrito y ejemplificado la naturaleza y objeto principal de la presente invención, como así también la manera en que la misma se puede llevar a la práctica, se declara reivindicar como de propiedad y de derechos exclusivos: UN PROCEDIMIENTO DE CULTIVO MASIVO DE CÉLULAS RECOMBINANTES DE MAMÍFERO PARA LA OBTENCIÓN DE ERITROPOYETINA HUMANA RECOMBINANTE, caracterizado porque el medio de cultivo productivo contiene insulina como único agregado proteico.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP980105611 AR008360A1 (es) | 1998-11-06 | 1998-11-06 | Procedimiento de cultivo masivo de celulas de mamifero para la obtencion de eritropoyetina humana recombinante y la eritropoyetina humana recombinante obtenida con tal procedimiento. |
| ARP990100681 AR008365A1 (es) | 1999-02-23 | 1999-02-23 | Procedimiento de cultivo masivo de celulas de mamifero para la obtencion de eritropoyetina humana recombinante y la eritropoyetina humana recombinanteobtenida con tal procedimiento |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO5241325A1 true CO5241325A1 (es) | 2003-01-31 |
Family
ID=25590828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO99069963A CO5241325A1 (es) | 1998-11-06 | 1999-11-05 | Procedimiento de cultivo masivo de celulas de mamifero para la obtencion de eritropoyetina humana recombinante y la eritropoyetina humana recombinante obtenida con tal procedimiento |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US7638296B1 (es) |
| EP (1) | EP1127104B1 (es) |
| JP (1) | JP2002529072A (es) |
| AT (1) | ATE413451T1 (es) |
| AU (1) | AU1609900A (es) |
| BR (1) | BR9905868A (es) |
| CA (1) | CA2350122A1 (es) |
| CO (1) | CO5241325A1 (es) |
| DE (1) | DE69939876D1 (es) |
| ES (1) | ES2315023T3 (es) |
| WO (1) | WO2000027997A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9917606A (pt) | 1998-11-06 | 2002-12-31 | Bio Sidus S A | Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento |
| US6777205B1 (en) | 1998-11-06 | 2004-08-17 | Sterrenbeld Biotechnologie North America, Inc. | Host cells expressing recombinant human erythropoietin |
| BR9905868A (pt) | 1998-11-06 | 2001-01-23 | Bio Sidus S A | Procedimento de cultivo massivo de células de mamìfero para a obtenção de eritropoetina humana, recombinante e a eritropoetina humana recombinante obtida com tal procedimento |
| US8084424B2 (en) | 2003-04-09 | 2011-12-27 | University Of Utah Research Foundation | Compositions and methods related to erythropoietin |
| JP5065391B2 (ja) * | 2006-12-06 | 2012-10-31 | 日本ケミカルリサーチ株式会社 | ヒトエリスロポエチンの製造方法 |
| DE102008054716A1 (de) * | 2008-12-16 | 2010-06-17 | Evonik Degussa Gmbh | Inprozesskontrolle in einem Verfahren zur Herstellung von EPO |
| WO2014138921A1 (en) | 2013-03-15 | 2014-09-18 | Apotex Inc. | Enhanced liquid formulation stability of erythropoietin alpha through purification processing |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3865801A (en) * | 1973-06-15 | 1975-02-11 | Atomic Energy Commission | Stabilization of urinary erythropoietin using sodium p-aminosalicylate and extracting into phenol |
| US5010002A (en) | 1983-01-19 | 1991-04-23 | Genentech, Inc. | Human t-PA production using vectors coding DHFR protein |
| KR850004274A (ko) | 1983-12-13 | 1985-07-11 | 원본미기재 | 에리트로포이에틴의 제조방법 |
| NZ210501A (en) | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| US4703008A (en) | 1983-12-13 | 1987-10-27 | Kiren-Amgen, Inc. | DNA sequences encoding erythropoietin |
| JPS6197229A (ja) | 1984-10-18 | 1986-05-15 | Chugai Pharmaceut Co Ltd | 安定なエリトロポエチン製剤 |
| IL77081A (en) | 1984-12-04 | 1999-10-28 | Genetics Inst | Dna sequence encoding human erythropoietin process for the preparation thereof and a pharmaceutical composition of human erythropoietin |
| US4677195A (en) * | 1985-01-11 | 1987-06-30 | Genetics Institute, Inc. | Method for the purification of erythropoietin and erythropoietin compositions |
| US4667016A (en) | 1985-06-20 | 1987-05-19 | Kirin-Amgen, Inc. | Erythropoietin purification |
| DK173067B1 (da) | 1986-06-27 | 1999-12-13 | Univ Washington | Humant erythropoietin-gen, fremgangsmåde til ekspression deraf i transficerede cellelinier, de transficerede cellelinier sa |
| KR100263845B1 (ko) * | 1989-10-13 | 2000-08-16 | 스튜어트 엘.왓트 | 에리트로포이에틴 동형체와 그의 제조방법 및 그를 포함하는제약학적 조성물 |
| US5256294A (en) * | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
| DE4115722A1 (de) * | 1991-05-14 | 1992-11-19 | Boehringer Mannheim Gmbh | Serumfreies medium zur kultivierung von saeugerzellen |
| SE9300105D0 (sv) | 1993-01-15 | 1993-01-15 | Kabi Pharmacia Ab | Stable protein solution |
| JPH0723780A (ja) * | 1993-07-12 | 1995-01-27 | Mitsui Toatsu Chem Inc | 有用物質生産用合成培地 |
| IL118201A (en) * | 1995-05-11 | 2004-12-15 | Roche Diagnostics Gmbh | Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof |
| WO1998000521A1 (fr) * | 1996-06-28 | 1998-01-08 | The Green Cross Corporation | Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives |
| US5876992A (en) | 1996-07-03 | 1999-03-02 | Molecular Biology Resources, Inc. | Method and formulation for stabilization of enzymes |
| CN1142281C (zh) * | 1998-01-19 | 2004-03-17 | 沈阳三生制药股份有限公司 | 重组人红细胞生成素制剂的制备生产方法 |
| BR9905868A (pt) | 1998-11-06 | 2001-01-23 | Bio Sidus S A | Procedimento de cultivo massivo de células de mamìfero para a obtenção de eritropoetina humana, recombinante e a eritropoetina humana recombinante obtida com tal procedimento |
| UY25790A1 (es) | 1998-11-06 | 2000-08-21 | Bio Sidus S A | Formas farmaceuticas de eritropoyetina humana recombinante estables a temperatura ambiente y apropiadas para su uso en humanos, formulaciones y procedimientos de liofilizacion para su obtencion. |
| BR9905867A (pt) | 1998-11-06 | 2001-01-23 | Bio Sidus S A | Linhagem celular produtora de eritropoietina humana recombinante e a eritropoietina humana recombinante produzida por esta célula |
| BR9917606A (pt) | 1998-11-06 | 2002-12-31 | Bio Sidus S A | Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento |
-
1999
- 1999-07-07 BR BR9905868-5A patent/BR9905868A/pt not_active Application Discontinuation
- 1999-11-05 CO CO99069963A patent/CO5241325A1/es not_active Application Discontinuation
- 1999-11-08 EP EP99958810A patent/EP1127104B1/en not_active Expired - Lifetime
- 1999-11-08 CA CA002350122A patent/CA2350122A1/en not_active Abandoned
- 1999-11-08 US US09/830,968 patent/US7638296B1/en not_active Expired - Fee Related
- 1999-11-08 WO PCT/US1999/026240 patent/WO2000027997A1/en not_active Ceased
- 1999-11-08 AU AU16099/00A patent/AU1609900A/en not_active Abandoned
- 1999-11-08 JP JP2000581164A patent/JP2002529072A/ja not_active Ceased
- 1999-11-08 AT AT99958810T patent/ATE413451T1/de not_active IP Right Cessation
- 1999-11-08 DE DE69939876T patent/DE69939876D1/de not_active Expired - Lifetime
- 1999-11-08 ES ES99958810T patent/ES2315023T3/es not_active Expired - Lifetime
-
2009
- 2009-12-28 US US12/647,849 patent/US20100210508A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000027997A1 (en) | 2000-05-18 |
| DE69939876D1 (de) | 2008-12-18 |
| BR9905868A (pt) | 2001-01-23 |
| AU1609900A (en) | 2000-05-29 |
| ES2315023T3 (es) | 2009-03-16 |
| US7638296B1 (en) | 2009-12-29 |
| EP1127104A1 (en) | 2001-08-29 |
| US20100210508A1 (en) | 2010-08-19 |
| EP1127104B1 (en) | 2008-11-05 |
| ATE413451T1 (de) | 2008-11-15 |
| EP1127104A4 (en) | 2005-04-27 |
| CA2350122A1 (en) | 2000-05-18 |
| JP2002529072A (ja) | 2002-09-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Application refused |