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BRPI1006435A8 - Molécula de ácido nucleico recombinante e célula hospedeira microbiana - Google Patents

Molécula de ácido nucleico recombinante e célula hospedeira microbiana

Info

Publication number
BRPI1006435A8
BRPI1006435A8 BRPI1006435A BRPI1006435A BRPI1006435A8 BR PI1006435 A8 BRPI1006435 A8 BR PI1006435A8 BR PI1006435 A BRPI1006435 A BR PI1006435A BR PI1006435 A BRPI1006435 A BR PI1006435A BR PI1006435 A8 BRPI1006435 A8 BR PI1006435A8
Authority
BR
Brazil
Prior art keywords
nucleic acid
host cell
acid molecule
recombinant nucleic
microbial host
Prior art date
Application number
BRPI1006435A
Other languages
English (en)
Inventor
E Apt Kirk
Simpson David
Richter Leslie
Zirkle Ross
Original Assignee
Martek Biosciences Corp
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martek Biosciences Corp, Dsm Ip Assets Bv filed Critical Martek Biosciences Corp
Publication of BRPI1006435A2 publication Critical patent/BRPI1006435A2/pt
Publication of BRPI1006435A8 publication Critical patent/BRPI1006435A8/pt
Publication of BRPI1006435B1 publication Critical patent/BRPI1006435B1/pt

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/0004—Oxidoreductases (1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
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    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
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    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01039—[Acyl-carrier-protein] S-malonyltransferase (2.3.1.39)
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    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
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    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01059—3-Hydroxyacyl-[acyl-carrier-protein] dehydratase (4.2.1.59)
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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  • Peptides Or Proteins (AREA)

Abstract

MOLÉCULA DE ÁCIDO NUCLEICO RECOMBINANTE E CÉLULA HOSPEDEIRA MICROBIANA. A presente invenção se refere a moléculas de ácido nucleico isoladas e polipeptídeos das sintases de ácidos graxos poli-insaturados (AGPl) de traustocitridios envolvidas na produção de AGPls, incluindo AGPls enriquecidos com ácido docosaexaenoico (DHA), ácido eicosapentaenoico (EPA), ou uma combinação destes. A presente invenção está direcionada para vetores e células hospedeiras compreendendo as moléculas de ácido nucleico, polipeptídeos codificados pelas moléculas de ácido nucleico, composições compreendendo as moléculas de ácido nucleico ou polipeptídeos, e métodos de produção e uso dos mesmos.
BRPI1006435-4A 2009-03-19 2010-03-19 molécula de ácido nucleico recombinante e célula hospedeira microbiana BRPI1006435B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16174209P 2009-03-19 2009-03-19
US61/161,742 2009-03-19
US29646010P 2010-01-19 2010-01-19
US61/296,460 2010-01-19
PCT/US2010/028009 WO2010108114A2 (en) 2009-03-19 2010-03-19 Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof

Publications (3)

Publication Number Publication Date
BRPI1006435A2 BRPI1006435A2 (pt) 2016-10-04
BRPI1006435A8 true BRPI1006435A8 (pt) 2017-09-26
BRPI1006435B1 BRPI1006435B1 (pt) 2021-01-19

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ID=42740260

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BRPI1006435-4A BRPI1006435B1 (pt) 2009-03-19 2010-03-19 molécula de ácido nucleico recombinante e célula hospedeira microbiana

Country Status (11)

Country Link
US (4) US8940884B2 (pt)
EP (2) EP2408797B1 (pt)
JP (3) JP2012521195A (pt)
CN (1) CN102741267B (pt)
AR (1) AR075897A1 (pt)
AU (1) AU2010226440B2 (pt)
BR (1) BRPI1006435B1 (pt)
CA (2) CA3012998C (pt)
ES (2) ES2717102T3 (pt)
TW (1) TWI600762B (pt)
WO (1) WO2010108114A2 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
CN105112463A (zh) 2000-01-28 2015-12-02 Dsmip资产公司 通过在发酵罐中高密度培养真核微生物来增加含有多烯脂肪酸的脂质的产生
WO2005120174A2 (en) * 2004-05-24 2005-12-22 Interactive Research Institute For Health Affairs Omega-3 fatty acid compositions with honey
ES2717102T3 (es) 2009-03-19 2019-06-19 Dsm Ip Assets Bv Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
DK2526197T3 (en) 2010-01-19 2018-10-01 Dsm Ip Assets Bv MICRO; PRODUCING EICOSAPENTAIC ACID AND DOCOSAHEXAENIC ACID, FATIC ACID COMPOSITIONS, AND METHODS FOR PREPARING AND USING THEREOF
TW201144442A (en) * 2010-05-17 2011-12-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
AU2015252107B2 (en) * 2011-03-07 2018-01-18 Dsm Nutritional Products Ag Engineering thraustochytrid microorganisms
DK2683824T3 (en) * 2011-03-07 2018-06-18 Dsm Nutritional Products Ag GENMANIPULATION OF THRAUSTOCHYTRIDE MICROORGANISMS
US20140323569A1 (en) * 2011-07-21 2014-10-30 Krishna Raman Microbial oils enriched in polyunsaturated fatty acids
BR122015020126B1 (pt) 2011-07-21 2022-03-03 Dsm Ip Assets B.V Composição contendo óleo microbiano diluído
TW201525136A (zh) * 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌ＰＵＦＡ合成酶於油籽作物中生成ω－３長鏈多不飽和脂肪酸
US10087430B2 (en) 2014-01-28 2018-10-02 Dsm Ip Assets B.V. Factors for the production and accumulation of polyunsaturated fatty acids (PUFAS) derived from PUFA synthases
EP3268457A4 (en) * 2015-03-12 2018-10-24 Synthetic Genomics, Inc. Microorganisms for fatty acid production using elongase and desaturase enzymes
EP3302032B1 (en) 2015-06-06 2021-12-15 DSM IP Assets B.V. Production of polyunsaturated fatty acids (pufas) using a novel modular docosahexaenoic acid (dha) synthase
KR102442450B1 (ko) * 2016-05-12 2022-09-14 디에스엠 아이피 어셋츠 비.브이. 미세조류에서 오메가-3 다중불포화 지방산 생산을 증가시키는 방법
KR102319845B1 (ko) 2016-07-13 2021-11-01 디에스엠 아이피 어셋츠 비.브이. 조류 숙주 세포에 대한 crispr-cas 시스템
US11248244B2 (en) 2017-05-10 2022-02-15 Nanjing University Of Technology Schizochytrium limacinum strain, building method therefor and application thereof
JP7039625B2 (ja) * 2017-05-31 2022-03-22 厦▲門▼▲匯▼盛生物有限公司 Ｄｈａおよびｅｐａを生産する細菌、細菌のゲノムの６つの遺伝子断片およびそれらの使用
CN107384806A (zh) * 2017-08-28 2017-11-24 厦门大学 一种基于同源重组的裂殖壶菌基因表达系统的构建及应用
EP3835410A4 (en) 2018-08-10 2022-05-18 Kyowa Hakko Bio Co., Ltd. MICROORGANISM PRODUCING EICOSAPENTAENOIC ACID AND METHOD FOR PRODUCTION OF EICOSAPENTAENOIC ACID
CA3249246A1 (en) 2022-01-25 2023-08-03 Dsm Ip Assets B.V. REFINEMENT OF MEDIA AND NUTRITION SUPPLY APPROACHES TO INCREASE POLYUNSATURATED FATTY ACID PRODUCTION
WO2025238190A1 (en) 2024-05-15 2025-11-20 Dsm Ip Assets B.V. Spray-dried powders having high pufa content

Family Cites Families (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
EP0116718B2 (en) 1983-01-13 1996-05-08 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Process for the introduction of expressible genes into plant cell genomes and agrobacterium strains carrying hybrid Ti plasmid vectors useful for this process
US6051757A (en) 1983-01-14 2000-04-18 Washington University Regeneration of plants containing genetically engineered T-DNA
EP0131624B1 (en) 1983-01-17 1992-09-16 Monsanto Company Plasmids for transforming plant cells
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
GB8317248D0 (en) 1983-06-24 1983-07-27 Wyeth John & Brother Ltd Fat compositions
NL8401048A (nl) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten.
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
AU7360087A (en) 1986-04-30 1987-11-24 Boyce Thompson Institute For Plant Research Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5246841A (en) 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
ES2121803T3 (es) 1987-05-20 1998-12-16 Novartis Ag Plantas de zea mays y plantas transgenicas de zea mays generadas a partir de protoplastos o celulas derivadas de protoplastos.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
ES2147551T3 (es) 1990-11-23 2000-09-16 Aventis Cropscience Nv Procedimiento para transformar plantas monocotiledoneas.
US5601860A (en) 1990-11-30 1997-02-11 American Home Products Corporation Corandomized fat compositions for infant formulas
US5141141A (en) 1990-12-21 1992-08-25 Nanette Leone Bag for use on the body
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
EP0616644B1 (en) 1991-12-04 2003-07-02 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
JPH07505531A (ja) 1992-04-15 1995-06-22 プラント・ジェネティック・システムズ・エヌ・ブイ 単子葉植物細胞の形質転換法
US5798259A (en) 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
US5683898A (en) 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
CA2113557A1 (en) 1992-05-15 1993-11-25 Kazunaga Yazawa Gene which codes for eicosapentaenoic acid synthetase group and process for producing eicosapentaenoic acid
DK0604662T3 (da) 1992-07-07 2008-10-20 Japan Tobacco Inc Fremgangsmåde til transformering af monocotyledon
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
DE69404385T2 (de) 1993-06-04 1998-02-19 Cavis Srl Trägheitsschalter zur Ausschaltung der Batterie eines Kraftfahrzeuges
DE4323727A1 (de) 1993-07-15 1995-03-09 Boehringer Mannheim Gmbh Verfahren zur Identifizierung von menschlichen und tierischen Zellen mit der Fähigkeit zu unbegrenzter Proliferation oder zur Tumorbildung
US5672491A (en) 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
EP0831149A4 (en) 1995-01-13 2000-12-06 Sagami Chem Res GENES CODING FOR THE GROUP OF ENZYMES FOR BIOSYNTHESIS OF EICOSAPENTIC ACID AND METHOD FOR PRODUCING EICOSAPENTIC ACID
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
DE69637953D1 (de) 1995-04-17 2009-07-30 Nat Inst Of Advanced Ind Scien Hoch ungesättigte fettsäurenproduzierende mikroorganismen und verfahren zur herstellung von hoch ungesättigten fettsäuren durch verwendung dieser mikroorganismen
RU2224795C2 (ru) 1995-10-13 2004-02-27 Дау Агросайенсез Ллс Модифицированный ген bacillus thuringiensis для борьбы с чешуекрылыми насекомыми в растениях
AR006830A1 (es) 1996-04-26 1999-09-29 Du Pont Aceite de soja con alta estabilidad oxidativa
EP0913473A4 (en) 1996-07-10 2000-12-06 Sagami Chem Res METHOD FOR PRODUCING EICOSAPENTIC ACID BY GENETIC RECOMBINATION
US6033883A (en) 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
CN1660065A (zh) 1997-03-27 2005-08-31 布里斯托尔-迈尔斯斯奎布公司 二十二碳六烯酸和花生四烯酸促进不足月婴儿生长的用途
SA98190002B1 (ar) 1997-03-28 2006-06-20 ذي يونيفيرسيتي اوف تننيسي ريسيرش كوربوريشن استخدام أحماض دهنية تحتوي على عدة روابط غير مشبعة polyunsaturated fatty acids لتقليل حدوث الالتهاب المعوي القولوني الناخر incidence of necrotizing enterocolitis
BR9808506A (pt) 1997-04-11 2000-05-23 Calgene Llc Métodos e composições para sìntese de ácidos graxos poliinsaturados de cadeia longa em plantas
US6051754A (en) 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
CA2283422A1 (en) 1997-06-04 1998-12-10 Calgene, Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US6013665A (en) 1997-12-16 2000-01-11 Abbott Laboratories Method for enhancing the absorption and transport of lipid soluble compounds using structured glycerides
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US7247461B2 (en) 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7211418B2 (en) 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
WO2001094565A2 (en) 2000-06-08 2001-12-13 Miami University FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES
US20040010817A1 (en) 2000-07-21 2004-01-15 Washington State University Research Foundation Plant acyl-CoA synthetases
DE60141760D1 (de) 2000-09-28 2010-05-20 Bioriginal Food & Science Corp Fad4, fad5, fad5-2, and fad6, mitglieder der fettsäuredesaturasefamilie und ihre verwendungen
TWI350854B (en) 2001-04-16 2011-10-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
TWI426127B (zh) * 2001-04-16 2014-02-11 Dsm Ip Assets Bv 多不飽和脂肪酸（ｐｕｆａ）聚乙醯合成酶系統及其用途（三）
WO2002094861A2 (en) 2001-05-21 2002-11-28 Ecopia Biosciences Inc. Genes and proteins involved in the biosynthesis of enediyne ring structures
US20040005672A1 (en) 2002-02-22 2004-01-08 Santi Daniel V. Heterologous production of polyketides
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
CA2519169C (en) 2002-03-16 2013-04-30 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
KR101234199B1 (ko) 2003-03-26 2013-02-20 마텍 바이오싸이언스스 코포레이션 Ｐｕｆａ 폴리케타이드 신타제 시스템 및 이의 용도
AU2004225838B2 (en) 2003-03-31 2009-11-05 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7208590B2 (en) 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
WO2005079367A2 (en) 2004-02-13 2005-09-01 Martek Biosciences Corporation Schizochytrium fatty acid synthase (fas) and products and methods related thereto
DE102004017370A1 (de) 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Gene aus Ulkenia
DE102004017369A1 (de) 2004-04-08 2005-11-03 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Screeningverfahren zur Identifizierung von PUFA-PKS in Proben
US20050255440A1 (en) * 2004-05-12 2005-11-17 Downing Linda P System and method of integrating levels of educational programs
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
EP2345730A1 (en) 2004-09-20 2011-07-20 BASF Plant Science GmbH Arabidopsis genes encoding proteins involved in sugar and lipid metabolism and methods of use
US7456340B2 (en) 2005-04-04 2008-11-25 Dow Agrosciences Llc Canola cultivar NQC02CNX21
US7355100B2 (en) 2004-09-30 2008-04-08 Dow Agrosciences Llc Canola cultivar NQC02CNX12
US7348473B2 (en) 2004-09-30 2008-03-25 Dow Agrosciences Llc Canola cultivar NQC02CNX25
GB0421937D0 (en) 2004-10-02 2004-11-03 Univ York Acyl CoA synthetases
WO2006125231A2 (en) 2005-05-17 2006-11-23 Photon Therapy Systems (Proprietary) Limited A photon therapy device
WO2006135866A2 (en) 2005-06-10 2006-12-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
DE112005003650T5 (de) 2005-08-11 2008-07-10 Fujitsu Ltd., Kawasaki Optisches Element und optisches Informationsaufzeichnungs-/-Wiedergabegerät
AU2013251201A1 (en) 2006-03-15 2013-11-21 Dsm Ip Assets B.V. Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
JP2009530622A (ja) 2006-03-15 2009-08-27 クゥアルコム・インコーポレイテッド 全地球的航法衛星システム
JP2009529891A (ja) 2006-03-15 2009-08-27 マーテック バイオサイエンシーズ コーポレーション 多価不飽和脂肪酸を含む植物種子油
ES2717102T3 (es) 2009-03-19 2019-06-19 Dsm Ip Assets Bv Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
DK2526197T3 (en) * 2010-01-19 2018-10-01 Dsm Ip Assets Bv MICRO; PRODUCING EICOSAPENTAIC ACID AND DOCOSAHEXAENIC ACID, FATIC ACID COMPOSITIONS, AND METHODS FOR PREPARING AND USING THEREOF
TW201525136A (zh) * 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌ＰＵＦＡ合成酶於油籽作物中生成ω－３長鏈多不飽和脂肪酸

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