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BRPI0924899B8 - Síntese hmo - Google Patents

Síntese hmo

Info

Publication number
BRPI0924899B8
BRPI0924899B8 BRPI0924899A BRPI0924899A BRPI0924899B8 BR PI0924899 B8 BRPI0924899 B8 BR PI0924899B8 BR PI0924899 A BRPI0924899 A BR PI0924899A BR PI0924899 A BRPI0924899 A BR PI0924899A BR PI0924899 B8 BRPI0924899 B8 BR PI0924899B8
Authority
BR
Brazil
Prior art keywords
cell
synthesis
transformed
nucleic acid
acid sequence
Prior art date
Application number
BRPI0924899A
Other languages
English (en)
Inventor
Jennewein Stefan
Hüfner Eric
Parkot Julia
Original Assignee
Jennewein Biotechnologie Gmbh
Chr Hansen Hmo Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40941939&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0924899(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Jennewein Biotechnologie Gmbh, Chr Hansen Hmo Gmbh filed Critical Jennewein Biotechnologie Gmbh
Publication of BRPI0924899A2 publication Critical patent/BRPI0924899A2/pt
Publication of BRPI0924899B1 publication Critical patent/BRPI0924899B1/pt
Publication of BRPI0924899B8 publication Critical patent/BRPI0924899B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SÍNTESE HMO. A presente invenção se refere a uma célula estavelmente cultivada em um meio, cuja célula é ajustada para a produção de oligossacarídeos, a célula sendo transformada para compreender pelo menos uma sequência de ácido nucléico que codifica uma enzima envolvida na síntese de oligossacarídeo. Além disto, a célula é transformada para compreender pelo menos uma sequência de ácido nucléico que codifica uma proteína da família de transportador de efluxo de açúcar, um homólogo funcional ou um derivado deste. Adicionalmente, a invenção inclui um método para a produção de oligossacarídeos que envolvem a célula acima descrita.
BRPI0924899A 2009-06-08 2009-06-08 Síntese hmo BRPI0924899B8 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2009/004112 WO2010142305A1 (en) 2009-06-08 2009-06-08 Hmo synthesis

Publications (3)

Publication Number Publication Date
BRPI0924899A2 BRPI0924899A2 (pt) 2021-02-23
BRPI0924899B1 BRPI0924899B1 (pt) 2022-03-22
BRPI0924899B8 true BRPI0924899B8 (pt) 2023-01-31

Family

ID=40941939

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0924899A BRPI0924899B8 (pt) 2009-06-08 2009-06-08 Síntese hmo

Country Status (15)

Country Link
US (2) US8652808B2 (pt)
EP (1) EP2440661B1 (pt)
JP (1) JP5580408B2 (pt)
CN (2) CN102459605A (pt)
AU (1) AU2009347610B2 (pt)
BR (1) BRPI0924899B8 (pt)
DK (1) DK2440661T3 (pt)
ES (1) ES2660698T3 (pt)
MX (1) MX2011013231A (pt)
MY (1) MY182355A (pt)
NZ (1) NZ597129A (pt)
PL (1) PL2440661T3 (pt)
RU (1) RU2517602C2 (pt)
SG (1) SG176651A1 (pt)
WO (1) WO2010142305A1 (pt)

Families Citing this family (72)

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MX349121B (es) 2010-06-02 2017-07-12 Evolva Inc Produccion recombinante de esteviol glucosidos.
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JP6262653B2 (ja) 2011-08-08 2018-01-17 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． ステビオール配糖体の組換え生産
CN102839157A (zh) * 2012-07-12 2012-12-26 扬州大学 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用
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KR20150115002A (ko) 2013-02-06 2015-10-13 에볼바 에스아 레바우디오시드 ｄ 및 레바우디오시드 ｍ의 개선된 생산 방법
MX2015010098A (es) 2013-02-11 2016-04-19 Evolva Sa Producción eficiente de glicosidos de esteviol en huéspedes recombinantes.
WO2014135167A1 (en) * 2013-03-08 2014-09-12 Glycom A/S Purification of oligosaccaharides by reversible derivatization
EP3041946B2 (en) 2013-09-06 2025-07-30 Glycom A/S Fermentative production of oligosaccharides
PL2845905T3 (pl) * 2013-09-10 2021-09-27 Chr. Hansen HMO GmbH Wytwarzanie oligosacharydów
EP2857410A1 (en) * 2013-10-04 2015-04-08 Jennewein Biotechnologie GmbH Process for purification of 2´-fucosyllactose using simulated moving bed chromatography
ES2701163T3 (es) 2014-01-20 2019-02-21 Jennewein Biotechnologie Gmbh Procedimiento para la purificación eficiente de oligosacáridos de la leche humana (HMO) neutros a partir de la fermentación microbiana
EP2905341B1 (en) * 2014-02-07 2020-04-29 Jennewein Biotechnologie GmbH Methods for producing GDP-fucose using nucleotide sugar transporters and recombinant microorganism host cells used therefor
EP3461890B1 (en) 2014-03-31 2025-09-24 Chr. Hansen HMO GmbH Total fermentation of oligosaccharides
CN114410493A (zh) 2014-08-11 2022-04-29 埃沃尔瓦公司 在重组宿主中生产甜菊醇糖苷
US10612064B2 (en) 2014-09-09 2020-04-07 Evolva Sa Production of steviol glycosides in recombinant hosts
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
EP3636077B1 (en) 2015-01-26 2022-11-16 Cadena Bio, Inc. Oligosaccharide compositions for use animal feed and methods of producing thereof
JP2018504142A (ja) * 2015-01-26 2018-02-15 カデナ・バイオ・インコーポレイテッド 食物成分として使用するためのオリゴ糖組成物及びその生成方法
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN108271391A (zh) * 2015-08-07 2018-07-10 埃沃尔瓦公司 重组宿主中的甜菊醇糖苷的产生
EP3141610A1 (en) * 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
WO2017178632A1 (en) 2016-04-13 2017-10-19 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN106190937B9 (zh) * 2016-07-18 2020-12-01 南开大学 一种构建重组大肠杆菌生物合成2’-岩藻乳糖的方法
DK3315610T3 (da) 2016-10-29 2021-03-08 Jennewein Biotechnologie Gmbh Fremgangsmåde til fremstilling af fucosylerede oligosaccharider
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP3604505A4 (en) * 2017-03-31 2020-02-19 Kaneka Corporation NOVEL MONOOXYGENASE AND USE THEREOF
WO2019003135A1 (en) 2017-06-30 2019-01-03 Glycom A/S PURIFICATION OF OLIGOSACCHARIDES
EP3425052A1 (en) 2017-07-07 2019-01-09 Jennewein Biotechnologie GmbH Fucosyltransferases and their use in producing fucosylated oligosaccharides
WO2019012461A1 (en) 2017-07-12 2019-01-17 Glycom A/S AMORPHOUS MIXTURE COMPRISING A NEUTRAL MONO- OR OLIGOSACCHARIDE AND A NON-GLUCIDIC ACID COMPONENT
EP3438122A1 (en) 2017-08-01 2019-02-06 OligoScience Biotechnology GmbH Microorganism for producing human milk oligosaccharide
KR20200084864A (ko) 2017-10-04 2020-07-13 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 면역조절성 올리고사카라이드
CN107805622B (zh) * 2017-11-08 2021-04-30 光明乳业股份有限公司 一种合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用
EP3486326A1 (en) 2017-11-21 2019-05-22 Jennewein Biotechnologie GmbH Method for the purification of n-acetylneuraminic acid from a fermentation broth
EP3784772A1 (en) * 2018-04-23 2021-03-03 DuPont Nutrition Biosciences ApS Increasing activity of 2' fucosyllactose transporters endogenous to microbial cells
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
EP3569713A1 (en) * 2018-05-16 2019-11-20 Jennewein Biotechnologie GmbH Use of glycosidases in the production of oligosaccharides
CN109735479B (zh) * 2019-01-30 2022-04-01 光明乳业股份有限公司 一种合成2’-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法与应用
EP3702468A1 (en) * 2019-03-01 2020-09-02 Jennewein Biotechnologie GmbH Fermentative production of carbohydrates by microbial cells utilizing a mixed feedstock
KR102254548B1 (ko) * 2019-04-26 2021-05-24 고려대학교 산학협력단 돌연변이 대장균을 이용한 콜라닉 산 생산
EP3751003A1 (en) 2019-06-12 2020-12-16 Jennewein Biotechnologie GmbH Production of fucosylated oligosaccharides in bacillus
WO2021013708A1 (en) * 2019-07-19 2021-01-28 Inbiose N.V. Production of fucosyllactose in host cells
BR112022002584A2 (pt) * 2019-08-13 2022-08-09 Amyris Inc Célula de levedura, método para produzir um ou mais oligossacarídeos, composição de fermentação, método para recuperar um ou mais oligossacarídeos, método para modificar geneticamente uma célula de levedura
CN110396532A (zh) * 2019-08-23 2019-11-01 中国科学院合肥物质科学研究院 一种制备唾液酸乳糖的方法
JP2022551195A (ja) * 2019-10-14 2022-12-07 インバイオス エン．フェー． 宿主細胞におけるバイオプロダクトの産生
BR112022011904A2 (pt) * 2019-12-18 2022-09-06 Inbiose Nv Produção de oligossacarídeo sialilado em células hospedeiras
WO2021148614A1 (en) 2020-01-23 2021-07-29 Glycom A/S Hmo production
BR112022014423A2 (pt) 2020-01-23 2022-09-27 Glycom As Produção de hmo
EP4093749A1 (en) 2020-01-23 2022-11-30 Glycom A/S Hmo production
BR112022014420A2 (pt) * 2020-01-23 2022-09-27 Glycom As Produção de hmo
JP2023519488A (ja) 2020-03-12 2023-05-11 グリコム・アクティーゼルスカブ ２’－ｆｌの結晶化
EP3929300A1 (en) 2020-06-26 2021-12-29 Chr. Hansen HMO GmbH Improved export of oligosaccharides from bacterial cells
DK180952B1 (en) 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
EP4281564A1 (en) 2021-01-22 2023-11-29 Glycom A/S New major facilitator superfamily (mfs) protein (fred) in production of sialylated hmos
US20240110212A1 (en) 2021-02-08 2024-04-04 Kyowa Hakko Bio Co., Ltd. Protein having fucose-containing sugar transporting activity, and method for producing fucose-containing sugar
EP4341277A1 (en) 2021-05-17 2024-03-27 DSM IP Assets B.V. Microbial strain expressing an invertase/sucrose hydrolase
DK181497B1 (en) 2021-05-17 2024-03-12 Dsm Ip Assets Bv ENHANCING FORMATION OF THE HMOS LNT AND/OR LNnT BY MODIFYING LACTOSE IMPORT IN THE CELL
DK181242B1 (en) 2021-05-17 2023-05-30 Dsm Ip Assets Bv GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING AN α-1,2-FUCOSYLTRANSFERASE CAPABLE OF PRODUCING LNFP-I, NUCLEIC ACID SEQUENCES ENCODING SAME AND METHODS FOR USE OF SAME
WO2023120615A1 (ja) 2021-12-21 2023-06-29 協和発酵バイオ株式会社 コア３糖としてラクト－ｎ－トリオースｉｉを含む糖質の製造方法および該糖質の結晶の製造方法
CN115029371B (zh) * 2022-06-14 2024-01-02 大连工业大学 一种微生物生产的天然活性产物的高效分离方法
DK181765B1 (en) 2022-07-15 2024-12-04 Dsm Ip Assets Bv Cells expressing new fucosyltransferases for in vivo synthesis of lnfp-iii, and methods and uses of same
CN120018780A (zh) 2022-08-25 2025-05-16 帝斯曼知识产权资产管理有限公司 生产复杂hmo的混合方法
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WO2025190861A1 (en) 2024-03-11 2025-09-18 Dsm Ip Assets B.V. Fucosyltransferase mutants with low dfl formation

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DK2440661T3 (en) * 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis

Also Published As

Publication number Publication date
MX2011013231A (es) 2012-01-20
WO2010142305A1 (en) 2010-12-16
ES2660698T3 (es) 2018-03-23
NZ597129A (en) 2012-12-21
BRPI0924899A2 (pt) 2021-02-23
US20140120611A1 (en) 2014-05-01
AU2009347610B2 (en) 2013-02-21
CN102459605A (zh) 2012-05-16
RU2517602C2 (ru) 2014-05-27
PL2440661T3 (pl) 2018-06-29
BRPI0924899B1 (pt) 2022-03-22
US20120135467A1 (en) 2012-05-31
US8652808B2 (en) 2014-02-18
DK2440661T3 (en) 2018-03-12
EP2440661B1 (en) 2017-12-06
JP5580408B2 (ja) 2014-08-27
MY182355A (en) 2021-01-20
RU2011153792A (ru) 2013-07-20
US9512433B2 (en) 2016-12-06
JP2012529274A (ja) 2012-11-22
EP2440661A1 (en) 2012-04-18
SG176651A1 (en) 2012-01-30
AU2009347610A1 (en) 2012-01-12
CN106978382A (zh) 2017-07-25

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/06/2009, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.

B25D Requested change of name of applicant approved

Owner name: CHR. HANSEN HMO GMBH (DE)