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BRPI0918570A2 - Exposição da superfície celular de isoformas de polipeptídeo mediante a interrupção da leitura completa do códon - Google Patents

Exposição da superfície celular de isoformas de polipeptídeo mediante a interrupção da leitura completa do códon

Info

Publication number
BRPI0918570A2
BRPI0918570A2 BRPI0918570-4A BRPI0918570A BRPI0918570A2 BR PI0918570 A2 BRPI0918570 A2 BR PI0918570A2 BR PI0918570 A BRPI0918570 A BR PI0918570A BR PI0918570 A2 BRPI0918570 A2 BR PI0918570A2
Authority
BR
Brazil
Prior art keywords
interrupting
cell surface
surface exposure
polypeptide isoforms
codon reading
Prior art date
Application number
BRPI0918570-4A
Other languages
English (en)
Inventor
Thomas Jostock
Burkhard Wilms
Audrey Josiane Nommay
Hans-Peter Knopf
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of BRPI0918570A2 publication Critical patent/BRPI0918570A2/pt
Publication of BRPI0918570B1 publication Critical patent/BRPI0918570B1/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BRPI0918570-4A 2008-08-28 2009-08-28 Ácido nucleico vetorial, seu método de produção, bem como métodos para selecionar pelo menos uma célula hospedeira eucariótica que expressa um polipeptídeo de interesse, e para produzir um polipeptídeo de interesse BRPI0918570B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08163161.6 2008-08-28
EP08163161 2008-08-28
PCT/EP2009/006246 WO2010022961A1 (en) 2008-08-28 2009-08-28 Cell surface display of polypeptide isoforms by stop codon readthrough

Publications (2)

Publication Number Publication Date
BRPI0918570A2 true BRPI0918570A2 (pt) 2015-08-11
BRPI0918570B1 BRPI0918570B1 (pt) 2024-04-02

Family

ID=

Also Published As

Publication number Publication date
IL211105A0 (en) 2011-04-28
RU2011111580A (ru) 2012-10-10
JP2012500637A (ja) 2012-01-12
CA2734340C (en) 2019-03-05
KR20110058850A (ko) 2011-06-01
PL2329020T3 (pl) 2013-08-30
CN102197136B (zh) 2016-09-28
US20110281751A1 (en) 2011-11-17
MX2011002273A (es) 2011-03-30
IL211105A (en) 2014-09-30
US10724033B2 (en) 2020-07-28
ES2413379T3 (es) 2013-07-16
JP5788796B2 (ja) 2015-10-07
DK2329020T3 (da) 2013-06-10
EP2329020B1 (en) 2013-03-13
KR101734585B1 (ko) 2017-05-11
WO2010022961A1 (en) 2010-03-04
PT2329020E (pt) 2013-06-20
CA2734340A1 (en) 2010-03-04
AU2009286956A1 (en) 2010-03-04
RU2528858C2 (ru) 2014-09-20
US9758779B2 (en) 2017-09-12
CN102197136A (zh) 2011-09-21
US20170342403A1 (en) 2017-11-30
AU2009286956B2 (en) 2012-12-06
EP2329020A1 (en) 2011-06-08

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 28/08/2009, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO.