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BRPI0516556A - certas plantas com nìveis "não saturados"ou reduzidos de ácidos graxos nas sementes e óleo derivado das sementes - Google Patents

certas plantas com nìveis "não saturados"ou reduzidos de ácidos graxos nas sementes e óleo derivado das sementes

Info

Publication number
BRPI0516556A
BRPI0516556A BRPI0516556-3A BRPI0516556A BRPI0516556A BR PI0516556 A BRPI0516556 A BR PI0516556A BR PI0516556 A BRPI0516556 A BR PI0516556A BR PI0516556 A BRPI0516556 A BR PI0516556A
Authority
BR
Brazil
Prior art keywords
present
seeds
unsaturated
plants
delta
Prior art date
Application number
BRPI0516556-3A
Other languages
English (en)
Inventor
Mark Thompson
Sam Reddy
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36148921&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0516556(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BRPI0516556A publication Critical patent/BRPI0516556A/pt
Publication of BRPI0516556B1 publication Critical patent/BRPI0516556B1/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings or cooking oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115Fatty acids or derivatives thereof; Fats or oils
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083Miscellaneous (1.14.99)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Edible Oils And Fats (AREA)
  • Fats And Perfumes (AREA)

Abstract

CERTAS PLANTAS COM NìVEIS "NãO SATURADOS" OU REDUZIDOS DE áCIDOS GRAXOS NAS SEMENTES E óLEO DERIVADO DAS SEMENTES. A presente invenção fornece óleo de canola "não saturado". A presente invenção fornece ainda sementes que podem ser utilizadas para produzir tais óleos. As plantas que produzem estas sementes também estão incluídas dentro da presente invenção. Tudo isso foi surpreendentemente conseguido através da utilização de um gene da delta-9 dessaturase na canola. Esta tecnologia pode ser aplicada a outras plantas como divulgado aqui. Os óleos da presente invenção possuem características particularmente vantajosas e perfis de ácidos graxos, que não tinham sido atingidos até agora. A presente invenção fornece ainda adicionalmente um gene da delta-9 dessaturase otimizado para plantas. A presente invenção fornece ainda adicionalmente um gene da delta-9 dessaturase otimizado para plantas. Em algumas modalidades preferidas, uma planta preferida compreende pelo menos duas cópias de um gene da delta-9 dessaturase da presente invenção. As sementes produzidas por tais plantas surpreendentemente não exibem efeitos de silenciamento gênico, mas ao invés disso possui ainda reduções surpreendentes nos níveis de saturados totais.
BRPI0516556A 2004-10-08 2005-10-07 composição alimentícia frita, processo de obtenção de uma composição alimentícia frita, processo de redução da gordura saturada na fração de óleo de sementes de uma planta transgênica de canola e polinucleotídeo BRPI0516556B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61753204P 2004-10-08 2004-10-08
PCT/US2005/036052 WO2006042049A2 (en) 2004-10-08 2005-10-07 Oil and seeds with reduced saturate levels of fatty acids

Publications (2)

Publication Number Publication Date
BRPI0516556A true BRPI0516556A (pt) 2008-09-09
BRPI0516556B1 BRPI0516556B1 (pt) 2019-12-31

Family

ID=36148921

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516556A BRPI0516556B1 (pt) 2004-10-08 2005-10-07 composição alimentícia frita, processo de obtenção de uma composição alimentícia frita, processo de redução da gordura saturada na fração de óleo de sementes de uma planta transgênica de canola e polinucleotídeo

Country Status (14)

Country Link
US (1) US20180030463A1 (pt)
EP (3) EP3318121A1 (pt)
JP (2) JP5072597B2 (pt)
CN (2) CN102978153A (pt)
AR (2) AR051932A1 (pt)
AU (1) AU2005294298C1 (pt)
BR (1) BRPI0516556B1 (pt)
CA (1) CA2583380C (pt)
DK (1) DK1799027T3 (pt)
ES (2) ES2388066T3 (pt)
HU (1) HUE034217T2 (pt)
MX (2) MX2007004231A (pt)
PL (2) PL1799027T3 (pt)
WO (1) WO2006042049A2 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2167667T3 (pl) 2007-07-09 2015-10-30 Bayer Cropscience Nv Roślina Brassica zawierająca zmutowane allele tłuszczowej tioesterazy acylo-ACP
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
CA2837011C (en) 2010-05-25 2023-01-17 Cargill Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
ES2620492T3 (es) * 2010-06-24 2017-06-28 Dow Agrosciences Llc Reducción del contenido de ácidos grasos saturados en semillas de plantas
TWI570239B (zh) 2010-06-24 2017-02-11 布魯克哈芬科學聯合有限責任公司 植物種子中ω-7脂肪酸的聚積技術
CN106998666A (zh) * 2014-12-19 2017-08-01 美国陶氏益农公司 低或无饱和脂肪酸的转基因卡诺拉
RU2018142876A (ru) * 2016-06-09 2020-07-09 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Способы и системы с применением ftir для выявления признаков растений и интрогрессии признаков
CN108342371B (zh) * 2018-04-12 2022-06-24 南京农业大学 一种新型阿魏酸酯酶及其编码基因和应用
CN113151318B (zh) * 2021-03-17 2022-08-16 云南中烟工业有限责任公司 一种烟草淀粉分支酶基因NtGBE1及其应用

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4627192B1 (en) 1984-11-16 1995-10-17 Sigco Res Inc Sunflower products and methods for their production
US5638637A (en) 1987-12-31 1997-06-17 Pioneer Hi-Bred International, Inc. Production of improved rapeseed exhibiting an enhanced oleic acid content
ES2063054T3 (es) 1987-12-31 1995-01-01 Pioneer Hi Bred Int Produccion de colza con contenido en acido oleico modificado.
US4948811A (en) 1988-01-26 1990-08-14 The Procter & Gamble Company Salad/cooking oil balanced for health benefits
US5057419A (en) 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
US6680426B2 (en) 1991-01-07 2004-01-20 Auburn University Genetic engineering of plant chloroplasts
US5932479A (en) 1988-09-26 1999-08-03 Auburn University Genetic engineering of plant chloroplasts
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5674485A (en) 1988-11-01 1997-10-07 The Regents Of The University Of California Insect diagnostic and control compositions with truncated JHE
US7037692B1 (en) 1990-03-16 2006-05-02 Calgene, Inc. Plant desaturases compositions and uses
DE69125991T2 (de) 1990-04-04 1997-09-25 Pioneer Hi Bred Int Herstellung von rapssamen mit verringertem gehalt an gesättigten fettsäuren
ATE195053T1 (de) 1990-08-30 2000-08-15 Cargill Inc Brassica-öle mit geänderten fettsäuregehalten
US5861187A (en) 1990-08-30 1999-01-19 Cargill, Incorporated Oil from canola seed with altered fatty acid profiles and a method of producing oil
ATE202144T1 (de) 1991-04-09 2001-06-15 Unilever Nv Pflanzen-promoter involviert in der kontrolle der lipid-biosynthese in samen
US6270828B1 (en) 1993-11-12 2001-08-07 Cargrill Incorporated Canola variety producing a seed with reduced glucosinolates and linolenic acid yielding an oil with low sulfur, improved sensory characteristics and increased oxidative stability
DE69225070T2 (de) 1991-09-30 1998-12-10 Cargill, Inc., Wayzata, Minn. Canolaöl mit niedrigem linolensäure- und schwefelgehalt
EP0616644B1 (en) 1991-12-04 2003-07-02 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
DE69333073T2 (de) 1992-02-12 2004-04-15 Chromagen, Inc., San Diego Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
DE69333034T2 (de) 1992-03-13 2004-04-01 Agrigenetics, Inc., San Diego Modifikation pflanzlicher Öle durch Verwendung von Desaturase
EP0928556A3 (en) * 1993-04-27 1999-09-15 Cargill, Incorporated Non-hydrogenated canola oil for food applications
US5912041A (en) * 1993-06-17 1999-06-15 Cargill, Incorporated Canola shortening for food applications
AU692791B2 (en) 1993-10-12 1998-06-18 Agrigenetics, Inc. Brassica napus variety AG019
ES2222462T3 (es) 1993-12-28 2005-02-01 Kirin Beer Kabushiki Kaisha Gen que codifica acido graso-desaturasa, vector que contiene dicho gen, planta que contiene dicho gen transferido a ella y procedimiento para crear dicha planta.
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5625130A (en) * 1995-03-07 1997-04-29 Pioneer Hi-Bred International, Inc. Oilseed Brassica bearing an endogenous oil wherein the levels of oleic, alpha-linolenic, and saturated fatty acids are simultaneously provided in an atypical highly beneficial distribution via genetic control
US6004782A (en) 1995-04-14 1999-12-21 Bioelastics Research Ltd. Hyperexpression of bioelastic polypeptides
ATE319296T1 (de) * 1995-12-14 2006-03-15 Cargill Inc Pflanzen mit mutierten sequenzen, welche ein veränderten fettsäuregehalt vermitteln
US5850026A (en) 1996-07-03 1998-12-15 Cargill, Incorporated Canola oil having increased oleic acid and decreased linolenic acid content
US6100450A (en) 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
US6586658B1 (en) 1998-03-06 2003-07-01 Metabolix, Inc. Modification of fatty acid metabolism in plants
US6495738B1 (en) * 1998-03-30 2002-12-17 Dow Agrosciences Llc Modification of fatty acid composition in plants by expression of a fungal acyl-CoA desaturase
EP1107975A4 (en) 1998-08-24 2003-07-16 Univ Rutgers SYNTHETIC FATTY ACID DESATURASEGEN FOR EXPRESSION IN PLANTS
DE60028053T2 (de) 1999-08-27 2006-12-21 SemBioSys Genetics, Inc., Calgary Samenspezifischer promoter aus flachs (linum usitatissimum)
DE19950589A1 (de) 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen
US6706950B2 (en) 2000-07-25 2004-03-16 Calgene Llc Nucleic acid sequences encoding β-ketoacyl-ACP synthase and uses thereof
WO2003014347A2 (en) 2000-12-01 2003-02-20 Michigan State University Plant seed specific promoters
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
US7294759B2 (en) 2001-06-29 2007-11-13 E. I. Du Pont De Nemours And Company Alteration of oil traits in plants
BR0309869A (pt) 2002-05-03 2005-04-26 Monsanto Technology Llc Promotores temporais de sementes para a expressão de genes em plantas

Also Published As

Publication number Publication date
EP1799027A2 (en) 2007-06-27
EP1799027A4 (en) 2008-08-27
EP2338328A3 (en) 2011-07-27
EP1799027B1 (en) 2012-06-20
US20180030463A1 (en) 2018-02-01
AR051932A1 (es) 2007-02-21
AR108902A2 (es) 2018-10-10
AU2005294298A1 (en) 2006-04-20
JP5753820B2 (ja) 2015-07-22
JP5072597B2 (ja) 2012-11-14
CA2583380C (en) 2016-08-02
ES2645787T3 (es) 2017-12-07
JP2012210217A (ja) 2012-11-01
WO2006042049A2 (en) 2006-04-20
DK1799027T3 (da) 2012-07-23
EP3318121A1 (en) 2018-05-09
CA2583380A1 (en) 2006-04-20
MX348676B (es) 2017-06-23
CN101065013A (zh) 2007-10-31
PL1799027T3 (pl) 2012-10-31
BRPI0516556B1 (pt) 2019-12-31
JP2008515433A (ja) 2008-05-15
ES2388066T3 (es) 2012-10-08
AU2005294298C1 (en) 2017-03-09
CN101065013B (zh) 2012-11-07
WO2006042049A3 (en) 2007-03-15
AU2005294298B2 (en) 2012-02-16
EP2338328B1 (en) 2017-09-20
MX2007004231A (es) 2007-06-14
PL2338328T3 (pl) 2018-02-28
CN102978153A (zh) 2013-03-20
EP2338328A2 (en) 2011-06-29
HUE034217T2 (en) 2018-01-29

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06H Technical and formal requirements: requirement cancelled [chapter 6.8 patent gazette]

Free format text: ANULACAO DA EXIGENCIA 6.6 POR TER SIDO INDEVIDA REFERENTE A RPI NO 2199 DE 26/02/2013.

B15K Others concerning applications: alteration of classification

Ipc: C12N 15/82 (2006.01), A23D 9/00 (2006.01), A23L 33

B06T Formal requirements before examination [chapter 6.20 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 31/12/2019, OBSERVADAS AS CONDICOES LEGAIS.

B25D Requested change of name of applicant approved

Owner name: CORTEVA AGRISCIENCE LLC (US)

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2743 DE 01-08-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.