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BR0012660A - Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal - Google Patents

Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal

Info

Publication number
BR0012660A
BR0012660A BR0012660-8A BR0012660A BR0012660A BR 0012660 A BR0012660 A BR 0012660A BR 0012660 A BR0012660 A BR 0012660A BR 0012660 A BR0012660 A BR 0012660A
Authority
BR
Brazil
Prior art keywords
personal care
composition
variants
protease variant
amino acid
Prior art date
Application number
BR0012660-8A
Other languages
English (en)
Inventor
Elizabeth Ellen Sikorski
Donn Nelton Rubingh
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Publication of BR0012660A publication Critical patent/BR0012660A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"VARIANTE DE PROTEASE TIPO SUBTILISINA; COMPOSIçãO DE LIMPEZA; E COMPOSIçãO DE CUIDADO PESSOAL". A presente invenção refere-se a variantes de proteases tipo subtilisina que têm imunogenicidade reduzida com relação a suas proteases do tipo selvagem correspondentes. Mais particularmente, a presente invenção refere-se a variantes que têm uma seq³ência de aminoácido modificada de uma seq³ência de aminoácido do tipo selvagem, em que a seq³ência de aminoácido modificada compreende uma substituição de uma ou mais regiões de epitopo. A invenção refere-se ainda a genes mutantes que codificam tais variantes e composições de limpeza e de cuidado pessoal que compreendem tais variantes.
BR0012660-8A 1999-07-22 2000-07-11 Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal BR0012660A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14498099P 1999-07-22 1999-07-22
PCT/US2000/018868 WO2001007578A2 (en) 1999-07-22 2000-07-11 Subtilisin protease variants having amino acid substitutions in defined epitope regions

Publications (1)

Publication Number Publication Date
BR0012660A true BR0012660A (pt) 2002-04-09

Family

ID=22511053

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0012660-8A BR0012660A (pt) 1999-07-22 2000-07-11 Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal

Country Status (11)

Country Link
US (1) US6586223B1 (pt)
EP (1) EP1210415A2 (pt)
JP (1) JP2003505070A (pt)
KR (1) KR20020021398A (pt)
CN (1) CN1399677A (pt)
AU (1) AU6085800A (pt)
BR (1) BR0012660A (pt)
CA (1) CA2376045A1 (pt)
CZ (1) CZ2002212A3 (pt)
MX (1) MXPA02000840A (pt)
WO (1) WO2001007578A2 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2441595C (en) 2001-03-23 2012-07-03 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
JP2005535284A (ja) 2001-12-31 2005-11-24 ジェネンコー・インターナショナル・インク 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法
EP1523553B1 (en) * 2002-01-16 2009-12-02 Genencor International, Inc. Multiply-substituted protease variants
US20050170488A1 (en) * 2002-01-16 2005-08-04 Poulose Ayrookaran J. Multiply-substituted protease variants
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
EP2500423B1 (en) 2003-02-26 2015-06-17 Danisco US Inc. Amylases producing an altered immunogenic response and methods of making and using the same
WO2004078960A1 (en) 2003-02-26 2004-09-16 Genencor International, Inc. Amylases producing an altered immunogenic response and methods of making and using the same
AU2003901917A0 (en) * 2003-04-22 2003-05-08 Novapharm Research (Australia) Pty Ltd Endoscope cleaning pad
US7824896B2 (en) 2004-04-02 2010-11-02 Novozymes A/S Subtilase variants having altered lmmunogenicity
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
MX2010013101A (es) 2008-06-06 2010-12-21 Danisco Inc Composiciones y metodos que comprenden proteasas microbianas variantes.
EP2589651A3 (en) * 2008-11-11 2013-08-28 Danisco US Inc. Compositions and methods comprising serine protease variants
CN103361196B (zh) 2008-11-11 2016-01-27 宝洁公司 包含一种或多种可组合的突变的芽孢杆菌属枯草杆菌蛋白酶
CA2775045A1 (en) * 2009-09-25 2011-03-31 Novozymes A/S Subtilase variants for use in detergent and cleaning compositions
CA2782891C (en) 2009-12-09 2022-01-11 Danisco Us Inc. Compositions and methods comprising protease variants
KR200453956Y1 (ko) * 2009-12-17 2011-06-13 진정욱 렌즈 고정구조를 갖는 반테안경
CN107002058B (zh) * 2014-12-15 2026-01-27 诺维信公司 枯草杆菌酶变体
CN109481336B (zh) * 2018-12-19 2021-10-26 广州立白企业集团有限公司 口腔护理组合物及其应用

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE795238A (fr) 1972-02-09 1973-08-09 Schering Ag Derives d'insuline reticules de maniere intramoleculaire, leur procede de preparation et leur utilisation
US4179337A (en) 1973-07-20 1979-12-18 Davis Frank F Non-immunogenic polypeptides
DE2631656C2 (de) 1976-07-14 1983-02-10 Boehringer Mannheim Gmbh, 6800 Mannheim Verwendung eines Hydroxysuccinimidoester-Derivats zur Bindung von biologisch aktiven Proteinen aneinander und/oder an flüssige oder feste Trägerstoffe
IT1162338B (it) 1978-07-04 1987-03-25 Novo Industri As Composizione a base di proteasi con ridotta allergenicita' e procedimento di preparazione
US4556554A (en) 1981-06-01 1985-12-03 Germaine Monteil Cosmetiques Corp. Immobilized enzymes
IE81141B1 (en) 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US4732863A (en) 1984-12-31 1988-03-22 University Of New Mexico PEG-modified antibody with reduced affinity for cell surface Fc receptors
US4844897A (en) 1985-09-13 1989-07-04 Hiroshi Maeda Anti-tumor protease preparations
WO1987004461A1 (en) 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US4908773A (en) 1987-04-06 1990-03-13 Genex Corporation Computer designed stabilized proteins and method for producing same
JPS63502959A (ja) 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
US4980288A (en) 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
SG30639G (en) 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ATE84313T1 (de) 1987-04-02 1993-01-15 Genentech Inc Substratunterstuetzte katalyse.
JP3155984B2 (ja) 1987-04-06 2001-04-16 ノヴォザイムズ・アクティーゼルスカブ タンパク質の安定化のための金属イオン結合部位における静電的相互作用の操作
US4853871A (en) 1987-04-06 1989-08-01 Genex Corporation Computer-based method for designing stablized proteins
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US5543302A (en) 1988-05-27 1996-08-06 Solvay Enzymes, Inc. Proteases of altered stability to autolytic degradation
JPH02219571A (ja) 1989-02-20 1990-09-03 Kanebo Ltd 修飾プロテアーゼ及びその製造法
US5122614A (en) 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5324844A (en) 1989-04-19 1994-06-28 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
AU618675B2 (en) 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins
ES2227508T3 (es) * 1989-06-26 2005-04-01 Unilever N.V. Composiciones detergentes enzimaticas.
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5230891A (en) 1990-08-20 1993-07-27 Kanebo Limited Modified protease, method of producing the same and cosmetic products containing the modified protease
US5133968A (en) 1990-08-20 1992-07-28 Kanebo, Ltd. Modified protease, method of producing the same and cosmetic products containing the modified protease
KR100237968B1 (ko) * 1990-12-05 2000-01-15 한센 핀 베네드 변화된 에피토프를 지닌 단백질 및 그 제조방법
US5766898A (en) 1990-12-05 1998-06-16 Novo Nordisk A/S Proteins with changed epitopes and methods for the production thereof
EP0516200B1 (en) 1991-05-01 1996-07-24 Unilever N.V. Detergent compositions containing stabilized enzymes
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5414135A (en) 1991-12-30 1995-05-09 Sterling Winthrop Inc. Vinyl sulfone coupling of polyoxyalkylenes to proteins
IT1260468B (it) 1992-01-29 1996-04-09 Metodo per mantenere l'attivita' di enzimi proteolitici modificati con polietilenglicole
GB9207288D0 (en) 1992-04-02 1992-05-13 Unilever Plc Cosmetic composition
GB9207280D0 (en) 1992-04-02 1992-05-13 Unilever Plc Skin care method and composition
CA2137378C (en) 1992-06-05 2008-08-05 Stephen Brian Henry Kent D-enzyme compositions and methods of their use
AU5006993A (en) 1992-08-21 1994-03-15 Enzon, Inc. Novel attachment of polyalkylene oxides to bio-effecting substances
CA2101361A1 (en) 1992-08-27 1994-02-28 Robert A. Snow Low diol polyalkylene oxide biologically active proteinaceous substances
US5567601A (en) 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
HUT74222A (en) 1993-09-15 1996-11-28 Procter & Gamble Subtilisin bpn' variants with decreased adsorption and increased hydrolysis
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
US6187579B1 (en) 1993-10-28 2001-02-13 Carlsberg A/S Customized proteases
US5446090A (en) 1993-11-12 1995-08-29 Shearwater Polymers, Inc. Isolatable, water soluble, and hydrolytically stable active sulfones of poly(ethylene glycol) and related polymers for modification of surfaces and molecules
ZA952220B (en) 1994-05-02 1995-12-14 Procter & Gamble Bpn' variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US5629384A (en) 1994-05-17 1997-05-13 Consiglio Nazionale Delle Ricerche Polymers of N-acryloylmorpholine activated at one end and conjugates with bioactive materials and surfaces
JPH10509324A (ja) 1994-11-24 1998-09-14 ノボ ノルディスク アクティーゼルスカブ 抑制されたアレルゲン性を有するポリペプチドの製造のための方法
FI972443A0 (fi) 1994-12-07 1997-06-09 Novo Nordisk As Polypeptidi, jonka allergeenisuus on vähentynyt
ATE257844T1 (de) 1995-03-10 2004-01-15 Nakamura Toshikazu Menschlicher wachstumsfaktor (hgf), verändert durch polyethylenglykol
WO1996040792A1 (en) 1995-06-07 1996-12-19 Novo Nordisk A/S Modification of polypeptides
WO1996040791A1 (en) 1995-06-07 1996-12-19 Novo Nordisk A/S Modification of polypeptides
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
WO1997024421A2 (en) 1995-12-29 1997-07-10 The Procter & Gamble Company Detergent compositions comprising immobilized enzymes
JPH11502256A (ja) 1995-12-29 1999-02-23 ザ、プロクター、エンド、ギャンブル、カンパニー 固定化酵素を含有する洗剤組成物
CN1273589C (zh) 1996-02-15 2006-09-06 诺沃奇梅兹有限公司 多肽缀合
US6946280B1 (en) 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same
US6060546A (en) * 1996-09-05 2000-05-09 General Electric Company Non-aqueous silicone emulsions
CN1246146A (zh) 1996-11-26 2000-03-01 金克克国际有限公司 化学修饰的酶
CA2277618A1 (en) 1997-01-10 1998-07-16 Novo Nordisk A/S Enzyme coupled with polymeric molecules for skin care
CN1246891A (zh) 1997-02-06 2000-03-08 诺沃挪第克公司 添加和/或除去了连接基团的多肽－聚合物偶联物
JP2002516615A (ja) 1997-06-25 2002-06-04 ノボザイムス アクティーゼルスカブ 変性ポリペプチド
MA25044A1 (fr) * 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
US5972339A (en) 1997-11-13 1999-10-26 The General Hospital Corporation Method of eliciting anti-HIV-1 helper T cell responses
GB9727262D0 (en) 1997-12-24 1998-02-25 Smithkline Beecham Biolog Vaccine
DK1047445T3 (da) 1998-01-23 2013-11-11 Univ Toronto Modificerede enzymer og anvendelse heraf til peptidsyntese
AU2674299A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Targeting of genetic vaccine vectors
IL137809A0 (en) 1998-02-12 2001-10-31 American Cyanamid Co Pneumococcal and meningococcal vaccines formulated with interleukin-12
US6451853B1 (en) 1998-02-24 2002-09-17 Dovetail Technologies, Inc Small molecules that elicit a polarized Th1 response and for modulation of cellular immunity
US5985264A (en) 1998-03-05 1999-11-16 The Medical College Of Ohio IL-12 Stimulation of Neonatal immunity
NZ506650A (en) 1998-03-05 2003-11-28 Ohio Med College Enhancement of immunity by intranasal inoculation of IL- 12
US6328967B1 (en) 1998-03-12 2001-12-11 Allergenics, Inc. Delivery system to modulate immune response
US6569663B1 (en) * 1998-03-26 2003-05-27 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
US6835550B1 (en) * 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
EP1088080B1 (en) 1998-06-24 2006-10-11 Genencor International, Inc. Recombinant production of cellulase from actinomycetes
WO2000028007A2 (en) 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
DK1141335T3 (da) 1998-12-21 2009-11-09 Genencor Int Kemisk modificerede enzymer med multipelt ladede varianter

Also Published As

Publication number Publication date
CA2376045A1 (en) 2001-02-01
EP1210415A2 (en) 2002-06-05
CN1399677A (zh) 2003-02-26
CZ2002212A3 (cs) 2002-07-17
MXPA02000840A (es) 2002-07-30
US6586223B1 (en) 2003-07-01
AU6085800A (en) 2001-02-13
WO2001007578A2 (en) 2001-02-01
WO2001007578A3 (en) 2002-04-11
KR20020021398A (ko) 2002-03-20
JP2003505070A (ja) 2003-02-12

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